Purification of α-amylase inhibitor from mulberry pomace by macroporous resin and analysis into its active components

doi:10.3969/j.issn.1004-1524.2019.07.16

›› 2019, Vol. 31 ›› Issue (7): 1154-1160.DOI: 10.3969/j.issn.1004-1524.2019.07.16

• Food Science • Previous Articles Next Articles

Purification of α-amylase inhibitor from mulberry pomace by macroporous resin and analysis into its active components

FAN Ming¹, XIANG Lu¹, XU Wenhui¹, CAO Yan², LIU Zhe², LU Shengmin^1,2,*

1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China;
2. Key Laboratory of Fruit and Vegetables Postharvest and Processing Technology of Zhejiang Province, Key Laboratory of Post-Harvest Handling of Fruit, Ministry of Agriculture and Rural Affairs, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2019-03-04 Online:2019-07-25 Published:2019-08-07

Abstract

Abstract: In this research, purification process of α-amylase inhibitors derived from mulberry pomace was optimized, and the effective substances was also studied. The effects of different macroporous resins on the static adsorption and desorption of α-amylase inhibitors were investigated. Furthermore, high performance liquid chromatography (HPLC) was applied to analyze the components of eluents obtained from macroporous resin by deionized water or ethanol solutions with different concentrations (10%, 30%, 50%, 70%, 90%). The results showed that AB-8 macroporous resin was the most optimal resin for separating α-amylase inhibitors. The optimum process parameters for dynamic adsorption and desorption of α-amylase inhibitors from mulberry pomace were as follows: sampling rate of 12 mL·min^-1, eluent of 70% ethanol solution, and elution flow rate of 24 mL·min^-1. It was found that the content of quercetin in the eluent varied accordingly with the α-amylase inhibitory activity of purified mulberry pomace extracts, thus it was inferred that quercetin might be the main contributor of α-amylase inhibitory activity of mulberry pomace extracts.

Key words: mulberry pomace, α-amylase inhibitors, purification, active ingredients, quercetin

CLC Number:

S663
O658

FAN Ming, XIANG Lu, XU Wenhui, CAO Yan, LIU Zhe, LU Shengmin. Purification of α-amylase inhibitor from mulberry pomace by macroporous resin and analysis into its active components[J]. , 2019, 31(7): 1154-1160.

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Purification of α-amylase inhibitor from mulberry pomace by macroporous resin and analysis into its active components

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