Cloning and sequence analysis of StBSKs gene of Solanum tuberosum

doi:10.3969/j.issn.1004-1524.2019.08.02

›› 2019, Vol. 31 ›› Issue (8): 1224-1230.DOI: 10.3969/j.issn.1004-1524.2019.08.02

• Crop Science • Previous Articles Next Articles

Cloning and sequence analysis of StBSKs gene of Solanum tuberosum

MA Jie^1,2, ZHENG Hao¹, ZHOU Ping², CHEN Chunyan², WU Rui², MA Wei², SONG Lei², SUN Bo^1,*

1. College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China;
2. Bijie Institute of Agricultural Science, Bijie 551700, China

Received:2019-04-08 Online:2019-08-25 Published:2019-08-30

Abstract

Abstract: Brassinolide (BR) is a kind of plant hormone that participates in the regulation of plant growth and environmental resistance. Brassinolide signal kinase (BSKs) is an important signal transduction kinase in BR signaling pathway. Solanum tuberosum used as material and specific primers were designed by homologous sequence retrieval. Seven genes of the StBSK gene family were cloned by RT-PCR technology, and respectively named StBSK1, StBSK2, StBSK3, StBSK4, StBSK5, StBSK6 and StBSK7. Total length of coding sequences were 1 497, 1 479, 1 464, 1 461, 1 476, 1 476 and 1 476 bp that encoded 498, 492, 487, 486, 491, 491 and 491 amino acid protein, respectively. Bioinformatics analysis showed that the isoelectric points of StBSKs protein were weakly acidic between 5.14 and 6.37, and these seven proteins had no transmembrane domain and signal peptide. Amino acid sequence alignment revealed that the amino acid sequences of StBSKs protein were different in N-terminal. Phylogenetic tree analysis showed that StBSKs protein was more closely related to the same species. The results of this study enriched the knowledge of the StBSKs gene and laid a foundation for further study on StBSKs gene function in Solanum tuberosum.

Key words: Solanum tuberosum, brassinolide signal kinase (BSK), amino acid sequence, phylogenetic tree

CLC Number:

S532

MA Jie, ZHENG Hao, ZHOU Ping, CHEN Chunyan, WU Rui, MA Wei, SONG Lei, SUN Bo. Cloning and sequence analysis of StBSKs gene of Solanum tuberosum[J]. , 2019, 31(8): 1224-1230.

References

[1] 罗其友, 刘洋, 高明杰, 等. 中国马铃薯产业现状与前景[J]. 农业展望, 2015, 11(3): 35-40.
LUO Q Y, LIU Y, GAO M J, et al.Status quo and prospect of China’s potato industry[J]. Agricultural Outlook, 2015, 11(3): 35-40.(in Chinese with English abstract).
[2] 何贤用, 杨松. 马铃薯主粮化与马铃薯全粉及其生产线[J]. 食品工业科技, 2015, 36(24): 378-379.
HE X Y, YANG S.Potato as a staple food, potato flour and its production line[J]. Science and Technology of Food Industry, 2015, 36(24): 378-379.(in Chinese with English abstract)
[3] 安娜, 董美, 宛煜嵩, 等. 转基因马铃薯的研究现状[J]. 中国农业科技导报, 2016, 18(4): 38-43.
AN N, DONG M, WAN Y S, et al.Research status of genetically modified potatoes[J]. Journal of Agricultural Science and Technology, 2016, 18(4): 38-43.(in Chinese with English abstract)
[4] 朱学玮. ZmBSK1在BR诱导的抗氧化防护过程中的作用[D]. 南京: 南京农业大学, 2012.
ZHU X W.The function of ZmBSK1 in BR-induced antioxidant defense[D]. Nanjing: Nanjing Agricultural University, 2012.(in Chinese with English abstract)
[5] SZEKERES M, NÉMETH K, KONCZ-KÁLMÁN Z, et al. Brassinosteroids rescue the deficiency of CYP90, a cytochrome P450, controlling cell elongation and de-etiolation in Arabidopsis[J]. Cell, 1996, 85(2): 171-182.
[6] BAJGUZ A, TRETYN A.The chemical characteristic and distribution of brassinosteroids in plants[J]. Phytochemistry, 2003, 62(7): 1027-1046.
[7] 李元元, 曹清河. 油菜素内酯参与调控植物生长发育与抗逆性的机制及其育种应用研究[J]. 中国农业科技导报, 2015, 17(2): 25-32.
LI Y Y, CAO Q H.Mechanism of brassinosteroid involved in regulating plant development, stress resistance and its application in breeding[J]. Journal of Agricultural Science and Technology, 2015, 17(2): 25-32.(in Chinese with English abstract)
[8] 高静, 张昊, 王凤茹, 等. 油菜素内酯提高水稻抗病性的分子机制[J]. 植物保护学报, 2016, 43(2): 347-348.
GAO J, ZHANG H, WANG F R, et al.The mechanism of enhancing disease resistance of rice(Oryza sativa) by brassinosteroid[J]. Journal of Plant Protection, 2016, 43(2): 347-348.(in Chinese)
[9] 张睿佳, 李瑛, 虞秀明, 等. 高温胁迫与外源油菜素内酯对‘巨峰’葡萄叶片光合生理和果实品质的影响[J]. 果树学报, 2015, 32(4): 590-596.
ZHANG R J, LI Y, YU X M, et al.Effects of heat stress and exogenous brassinolide on photosynthesis of leaves and berry quality of‘Kyoho’ grapevine[J]. Journal of Fruit Science, 2015, 32(4): 590-596.(in Chinese with English abstract)
[10] 魏鑫, 倪虹, 张会慧, 等. 外源脱落酸和油菜素内酯对干旱胁迫下大豆幼苗抗旱性的影响[J]. 中国油料作物学报, 2016, 38(5): 605-610.
WEI X, NI H, ZHANG H H, et al.Effects of exogenous abscisic acid and brassinolide on drought resistance of soybean seedlings[J]. Chinese Journal of Oil Crop Sciences, 2016, 38(5): 605-610.(in Chinese with English abstract)
[11] ANURADHA S, SEETA RAM RAO S. Effect of brassinosteroids on salinity stress induced inhibition of seed germination and seedling growth of rice (Oryza sativa L.)[J]. Plant Growth Regulation, 2001, 33(2): 151-153.
[12] 王舒甜, 张金池, 张亮. 油菜素内酯对盐胁迫下香樟叶片光合色素以及叶绿素荧光的影响[J]. 浙江大学学报(农业与生命科学版), 2017, 43(1): 45-53.
WANG S T, ZHANG J C, ZHANG L.Effects of exogenous 24-epibrassinolide on chlorophyll content and chlorophyll fluorescence characteristics of camphor seedlings under salt stress[J]. Journal of Zhejiang University(Agriculture & Life Sciences), 2017, 43(1): 45-53.(in Chinese with English abstract)
[13] 时华. 拟南芥胞质型类受体激酶BSK1调控植物先天免疫反应[D]. 北京: 中国科学院大学, 2013.
SHI H.BSK1, a receptor-like cytoplasmic kinase, regulate innate immunity in Arabidopsis[D]. Beijing: University of Chinese Academy of Sciences, 2013. (in Chinese with English abstract)
[14] 胡雪微. ZmBSKs/OsBSKs家族激酶鉴定及OsBSK3功能研究[D]. 大庆: 黑龙江八一农垦大学, 2015.
HU X W.Analysis of ZmBSKs/OsBSKs kinase family identification and OsBSK3 function[D]. Daqing: Heilongjiang Bayi Agricultural University, 2015.(in Chinese with English abstract)
[15] CHEN Q, YU H W, WANG X R, et al.Methodology: an alternative cetyltrimethylammonium bromide-based protocol for RNA isolation from blackberry (Rubus L.)[J]. Genetics and Molecular Research, 2012, 11(2): 1773-1782.
[16] 束红梅, 郭书巧, 巩元勇, 等. 西红花CCD家族基因序列分析及表达特性[J]. 江苏农业学报, 2017, 33(2): 417-423.
SHU H M, GUO S Q, GONG Y Y, et al.Sequence analysis and expression characteristics of carotenoid cleavage dioxygenases (CCD) genes in Crocus sativus[J]. Jiangsu Journal of Agricultural Sciences, 2017, 33(2): 417-423.(in Chinese with English abstract)
[17] 李慧, 丛郁, 常有宏, 等. 杜梨CPI基因的克隆、序列分析及表达[J]. 江苏农业学报, 2011, 27(5): 1070-1077.
LI H, CONG Y, CHANG Y H, et al.Cloning, sequencing and expression of a cysteine proteinase inhibitor gene (PbCPI) from Pyrus betulaefolia Bunge[J]. Jiangsu Journal of Agricultural Sciences, 2011, 27(5): 1070-1077.(in Chinese with English abstract)
[18] TANG W, KIM T W, OSES-PRIETO J A, et al. BSKs mediate signal transduction from the receptor kinase BRI1 in Arabidopsis[J]. Science, 2008, 321(5888): 557-560.
[19] 胡雪微, 杨克军, 李佐同, 等. 玉米BSKs基因家族生物信息学分析[J]. 植物生理学报, 2015, 51(4): 528-536.
HU X W, YANG K J, LI Z T, et al.Genome-wide analysis of the BSKs gene family in maize (Zea mays)[J]. Plant Physiology Journal, 2015, 51(4): 528-536.(in Chinese with English abstract)

Cloning and sequence analysis of StBSKs gene of Solanum tuberosum

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