Screening of internal reference gene of Agaricus bisporus

doi:10.3969/j.issn.1004-1524.2019.08.12

›› 2019, Vol. 31 ›› Issue (8): 1312-1320.DOI: 10.3969/j.issn.1004-1524.2019.08.12

• Horticultural Science • Previous Articles Next Articles

Screening of internal reference gene of Agaricus bisporus

ZHAO Jianxia^1,2, SHEN Yingyue¹, FENG Weilin¹, JIN Qunli¹, SONG Tingting¹, FAN Lijun¹, CAI Weiming^1,*

1. Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China

Received:2019-05-26 Online:2019-08-25 Published:2019-08-30

Abstract

Abstract: According to specific experimental materials and conditions, the selection of appropriate internal reference genes in RT-qPCR analysis was crucial for accurate correction of target genes expression. In present study, eight internal genes were used to analyze expression stability between five development stages, three different tissues or organs, four different temperature by GeNorm, NormFiner softwares and Δ Ct methed. The results showed that the optimal internal reference genes of Agaricus bisporus at different developmental stages, in different tissues and at different temperatures were 40s+actin+Eif5 combination, Eif5+ubiquitin+PRL14 combination and EF1+ubiquitin+RPL14 combination, respectively. This study had important practical value for the quantitative expression analysis of key genes related with developmet and temperatural response in Agaricus bisporus.

Key words: real time quantitative PCR, internal reference genes, expression stability, Agaricus bisporus

CLC Number:

S646

ZHAO Jianxia, SHEN Yingyue, FENG Weilin, JIN Qunli, SONG Tingting, FAN Lijun, CAI Weiming. Screening of internal reference gene of Agaricus bisporus[J]. , 2019, 31(8): 1312-1320.

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Screening of internal reference gene of Agaricus bisporus

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