Effects of DMSO and DMF on myocardial cytotoxicity and STAT3 signaling pathway in H9C2

doi:10.3969/j.issn.1004-1524.2019.09.07

›› 2019, Vol. 31 ›› Issue (9): 1446-1452.DOI: 10.3969/j.issn.1004-1524.2019.09.07

• Animal Science • Previous Articles Next Articles

Effects of DMSO and DMF on myocardial cytotoxicity and STAT3 signaling pathway in H9C2

YANG Min, ZHANG Kai, LI Jianxi, WANG Lei, ZHANG Kang, QIU Zhengying, WANG Xuezhi^*

Lanzhou Institute of Animal Husbandry and Veterinary Drugs, Chinese Academy of Agricultural Sciences, Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou 730050, China

Received:2019-02-14 Online:2019-09-25 Published:2019-10-11

Abstract

Abstract: In order to screen the lowest concentration of toxic effects of organic solvents DMSO and DMF on H9C2 cardiomyocytes, and to investigate the effects of DMSO and DMF on signal transduction and transcription factor 3 (signal transducer and activator of transcription 3, STAT3) signal pathway, the survival rates of cardiomyocytes exposed to DMSO and DMF at volume fraction of 0.1%, 0.2%, 0.3%, 0.5%, 0.7%, 0.9%, 1%, 2%, 3% for 24 h, 48 h and 72 h, respectively, were detected by the tetrazolium salt colorimetric assay (MTT method). And the survival rate of STAT3 and Bcl-2 protein was determined by Western blot method, and the expression of STAT3 protein was compared between DMSO and DMF at 0%, 0.2%, 0.3%, 0.5% volume fraction, respectively. At the same time, the survival rate of cardiomyocytes decreased with the increase of the volume percentage of DMSO and DMF, and the content of Bcl-2 and STAT3 protein decreased. When the volume fraction of DMSO was 0.3% and 0.5%, the cell survival rate was significantly lower than that of the control group (P<0.05). The protein levels of STAT3 and Bcl-2 were lower than those of the control group (P<0.05). The survival rate of cardiomyocytes decreased with the increase of the volume percentage of DMSO and DMF, and the content of Bcl-2 and STAT3 protein decreased at the same time. However, when the volume fraction of DMSO was 0.2%, there was no significant difference in the cell survival rate and the expression of STAT3 and Bcl-2 compared with the control group (P>0.05). When the volume fraction of DMF was 0.3% and 0.5%, the cell survival rate was statistically significant (P<0.05), and the protein levels of STAT3 and Bcl-2 were lower than those in the control group. When the volume fraction of DMF was 0.2%, the cell survival rate was not statistically significant (P>0.05). The expression of STAT3 protein was significantly higher than that in the control group (P<0.01). Compared with the control group (without DMSO and DMF), DMSO and DMF had toxic effects on H9C2 cardiomyocytes. With the increase of concentration, the cell survival rate decreased and the content of Bcl-2 protein decreased, which may be related to the down-regulation of STAT3 protein. The results of MTT assay were not completely consistent with those of Western blot test, and MTT assay was unstable.

Key words: H9C2 cardiomyocytes, DMSO, DMF, MTT, STAT3

CLC Number:

S853.7

YANG Min, ZHANG Kai, LI Jianxi, WANG Lei, ZHANG Kang, QIU Zhengying, WANG Xuezhi. Effects of DMSO and DMF on myocardial cytotoxicity and STAT3 signaling pathway in H9C2[J]. , 2019, 31(9): 1446-1452.

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Effects of DMSO and DMF on myocardial cytotoxicity and STAT3 signaling pathway in H9C2

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