Diversities of microbial communities in midgut of 3 planthoppers species by MiSeq

doi:10.3969/j.issn.1004-1524.2020.12.10

Acta Agriculturae Zhejiangensis ›› 2020, Vol. 32 ›› Issue (12): 2192-2200.DOI: 10.3969/j.issn.1004-1524.2020.12.10

• Plant Protection • Previous Articles Next Articles

Diversities of microbial communities in midgut of 3 planthoppers species by MiSeq

ZHANG Juefeng¹(), YU Yefei², LI Fang¹, ZHONG Haiying¹, CHEN Jianming¹^,^*()

1. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. Dapanshan National Nature Reserve Administration of Zhejiang, Pan’an, 322300, China;

Received:2020-03-20 Online:2020-12-25 Published:2020-12-25
Contact: CHEN Jianming

Abstract

Abstract:

In this study, we successfully demonstrated that MiSeq was a powerful approach for investigating the bacterial communities in midgut of brown planthopper (BPH) Nilaparvata lugens St?l, white-backed planthopper (WBPH) Sogatella furcifera Horvath and small brown planthopper(SBPH)Laodelphax striatellus(Fallen). For all three planthoppers, 3.44×10 ⁵ effective sequences were selected and utilized for the bacterial diversity and abundance analysis. In total, 76, 103, and 61 operational taxonomic units were obtained at 3% distance cutoff, 49, 68 and 42 at 6% distance cutoff in the BPH, SBPH and WBPH, respectively. Nearly 90% of the sequences in the SBPH were assigned into F-proteobacteria-unclassified. In the other two samples, Arsenophonus was the dominant genus in BPH, accounting for about about 70% sequences, while in WBPH, Halomonas, Candidatus_Cardinium and Arsenophonus were 3 dominant bacterias, accounting for about 35%, 20% and 15%, respectively. Though they were evolved from the common ancestor, the microbial community diversity, richness, dominant bacteria function of endosymbiont in three planthoppers were different.

Key words: brown planthopper (BPH), white-backed planthopper (WBPH), small brown planthopper (SBPH), MiSeq, bacterial communities

CLC Number:

S433.3

ZHANG Juefeng, YU Yefei, LI Fang, ZHONG Haiying, CHEN Jianming. Diversities of microbial communities in midgut of 3 planthoppers species by MiSeq[J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2192-2200.

Figures/Tables 6

References 36

[1]	BAUMANN P. Biology of bacteriocyte-associated endosymbionts of plant Sap-sucking insects[J]. Annual Review of Microbiology, 2005,59(1):155-189. DOI URL
[2]	KIKUCHI Y, HAYATSU M, HOSOKAWA T, et al. Symbiont-mediated insecticide resistance[J]. PNAS, 2012,109(22):8618-8622. DOI URL PMID
[3]	周岩岩, 董胜张, 白旭, 等. 三种稻飞虱体内类酵母共生菌18S rDNA和ITS-5.8S rDNA序列克隆及进化分析[J]. 昆虫学报, 2012,55(4):482-487.
	ZHOU Y Y, DONG S Z, BAI X, et al. Phylogenetic position of yeast-like Symbiotes from three rice planthoppers (Homoptera: Delphacidae) based on 18S rDNA and ITS-5.8S rDNA sequences[J]. Acta Entomologica Sinica, 2012,55(4):482-487.(in Chinese with English abstract)
[4]	MCCUTCHEON J P. The bacterial essence of tiny symbiont genomes[J]. Current Opinion in Microbiology, 2010,13(1):73-78. DOI URL PMID
[5]	ENGELSTÄDTER J, HURST G D D. The ecology and evolution of microbes that manipulate host reproduction[J]. Annual Review of Ecology, Evolution and Systematics, 2009,40(1):127-149.
[6]	WANG W X, ZHU T H, LAI F X, et al. Diversity and infection frequency of symbiotic bacteria in different populations of the rice brown planthopper in China[J]. Journal of Entomological Science, 2015,50(1):47-66. DOI URL
[7]	TANG M, LV L, JING S L, et al. Bacterial symbionts of the brown planthopper, Nilaparvata lugens(Homoptera: Delphacidae)[J]. Applied and Environmental Microbiology, 2010,76(6):1740-1745. URL PMID
[8]	XU H X, ZHENG X S, YANG Y J, et al. Changes in endosymbiotic bacteria of brown planthoppers during the process of adaptation to different resistant rice varieties[J]. Environmental Entomology, 2015,44(3):582-587. DOI URL PMID
[9]	李汝铎. 褐飞虱及其种群管理[M]. 上海: 复旦大学出版社, 1996.
[10]	相辉, 李木旺, 赵勇, 等. 家蚕幼虫中肠细菌群落多样性的PCR-DGGE和16S rDNA文库序列分析[J]. 昆虫学报, 2007,50(3):222-233.
	XIANG H, LI M W, ZHAO Y, et al. Bacterial community in midguts of the silkworm larvae estimated by PCR/DGGE and 16S rDNA gene library analysis[J]. Acta Entomologica Sinica, 2007,50(3):222-233.(in Chinese with English abstract)
[11]	OLIVER K M, MORAN N A, HUNTER M S. Variation in resistance to parasitism in aphids is due to symbionts not host genotype[J]. Proceedings of the National Academy of Sciences, 2005,102(36):12795-12800. DOI URL
[12]	LYTE M, GAYKEMA R P A, GOEHLER L E. Behavior modification of host by microbes[M] //SCHAECHTER M. Encyclopedia of Microbiology. Amsterdam: Elsevier, 2009: 121-127.
[13]	WATANABE K, SATO M. Gut colonization by an ice nucleation active bacterium, Erwinia(Pantoea) ananas reduces the cold hardiness of mulberry pyralid larvae[J]. Cryobiology, 1999,38(4):281-289. DOI URL PMID
[14]	徐红星, 郑许松, 杨亚军, 等. 褐飞虱体内细菌群落的PCR-DGGE分析[J]. 中国水稻科学, 2014,28(2):217-222. DOI URL
	XU H X, ZHENG X S, YANG Y J, et al. PCR-DGGE analysis of the bacterial community in different populations of rice brown planthopper, Nilaparvata lugens stl[J]. Chinese Journal of Rice Science, 2014,28(2):217-222.(in Chinese with English abstract) DOI URL
[15]	张开军. 稻飞虱与其体内寄生菌Wolbachia和Cardinium的分子进化关系研究[D]. 南京: 南京农业大学, 2013.
	ZHANG K J. The molecular evolutionary relationships between rice planthoppers and their endosymbionts Wolbachia and Cardinium[D]. Nanjing: Nanjing Agricultural University, 2013.(in Chinese with English abstract)
[16]	NODA H, MIYOSHI T, ZHANG Q, et al. Wolbachia infection shared among planthoppers (Homoptera: Delphacidae) and their endoparasite (Strepsiptera: Elenchidae): a probable case of interspecies transmission[J]. Molecular Ecology, 2001,10(8):2101-2106. DOI URL PMID
[17]	屈吕宇, 楼怡寒, 黄海剑, 等. 亚洲不同地理种群褐飞虱内共生菌Wolbachia的分子检测[J]. 应用昆虫学报, 2013,50(5):1320-1327.
	QU L Y, LOU Y H, HUANG H J, et al. Molecular detection of the endosymbiont Wolbachia in different Asian populations of the brown planthopper[J]. Chinese Journal of Applied Entomology, 2013,50(5):1320-1327.(in Chinese with English abstract)
[18]	NAKAMURA Y, YUKUHIRO F, MATSUMURA M, et al. Cytoplasmic incompatibility involving Cardinium and Wolbachia in the white-backed planthopper Sogatella furcifera(Hemiptera: Delphacidae)[J]. Applied Entomology and Zoology, 2012,47(3):273-283. DOI URL
[19]	SKINNER S W. Son-killer: a third extrachromosomal factor affecting the sex ratio in the parasitoid wasp, Nasonia(=Mormoniella) vitripennis[J]. Genetics, 1985,109(4):745-759. URL PMID
[20]	GHERNA R L, WERREN J H, WEISBURG W, et al. NOTES: Arsenophonus nasoniae gen. nov., sp. nov., the causative agent of the son-killer trait in the parasitic wasp Nasonia vitripennis[J]. International Journal of Systematic Bacteriology, 1991,41(4):563-565. DOI URL
[21]	阮永明, 刘树生. 浙江B型与非B型(China-ZHJ-1)烟粉虱种群共生细菌的检测及系统发育分析[J]. 昆虫学报, 2005,48(6):859-865.
	RUAN Y M, LIU S S. Detection and phylogenetic analysis of prokaryotic endosymbionts in Bemisia tabaci[J]. Acta Entomologica Sinica, 2005,48(6):859-865.(in Chinese with English abstract)
[22]	HADDADI A, SHAVANDI M. Biodegradation of phenol in hypersaline conditions by Halomonas sp. strain PH₂-2 isolated from saline soil[J]. International Biodeterioration & Biodegradation, 2013,85:29-34.
[23]	CORSINI A, ZACCHEO P, MUYZER G, et al. Arsenic transforming abilities of groundwater bacteria and the combined use of Aliihoeflea sp. strain 2WW and goethite in metalloid removal[J]. Journal of Hazardous Materials, 2014,269:89-97. DOI URL
[24]	DAI Y J, ZHAO Y J, ZHANG W J, et al. Biotransformation of thianicotinyl neonicotinoid insecticides: diverse molecular substituents response to metabolism by bacterium Stenotrophomonas maltophilia CGMCC 1.1788[J]. Bioresource Technology, 2010,101(11):3838-3843. DOI URL PMID
[25]	ZHAO Y J, DAI Y J, YU C G, et al. Hydroxylation of thiacloprid by bacterium Stenotrophomonas maltophilia CGMCC1.1788[J]. Biodegradation, 2009,20(6):761-768. URL PMID
[26]	刘叙杆, 赵兴华, 王彦华, 等. 褐飞虱对氟虫腈和新烟碱类药剂的抗性动态变化[J]. 中国水稻科学, 2010,24(1):73-80. DOI URL
	LIU X G, ZHAO X H, WANG Y H, et al. Dynamic changes of resistance to fipronil and neonicotinoid insecticides in brown planthopper, Nilaparvata lugens(Homoptera: Delphacidae)[J]. Chinese Journal of Rice Science, 2010,24(1):73-80.(in Chinese with English abstract) DOI URL
[27]	SALTER S, COX M J, TUREK E M, et al. Reagent contamination can critically impact sequence based microbiome analyses[J]. BMC Biology, 2014,12:87. DOI URL PMID
[28]	XU X W, HUO Y Y, WANG C S, et al. Pelagibacterium halotolerans gen. nov., sp. nov. and Pelagibacterium luteolum sp. nov., novel members of the family Hyphomicrobiaceae[J]. International Journal of Systematic and Evolutionary Microbiology, 2011,61(8):1817-1822. DOI URL
[29]	GE S M, AI W J, DONG X J. High-quality draft genome sequence of Leucobacter sp. strain G161, a distinct and effective chromium reducer[J]. Genome Announcements, 2016,4(1):e01760-15. DOI URL PMID
[30]	ALEXANDER S K, STRETE D, MADIGAN M T. Brock: biology of microorganisms (13th Ed) with microbiology: a photographic atlas for the laboratory[M]. Value Pack: Pearson Schweiz Ag, 2010.
[31]	李永. 健康与染溃疡病107杨、中林46杨树皮真菌和细菌群落研究[D]. 北京: 北京林业大学, 2013.
	LI Y. Fungal and bacterial communities in healthy and canker-infected bark of Populus × euramericana cv.‘74/76’and P. × euramericana cv.‘Zhonglin46’[D]. Beijing: Beijing Forestry University, 2013.(in Chinese with English abstract)
[32]	张婧. 次生内共生菌Rickettsia在烟粉虱MED隐种内的分布、传播以及对宿主生殖影响研究[D]. 重庆: 西南大学, 2017.
	ZHANG J. The distribution, transmission, and effects on the host reproduction of Rickettsia in Bemisia tabaci MED[D]. Chongqing: Southwest University, 2017.(in Chinese with English abstract)
[33]	TAN S Y, DUTTA A, JAKUBOVICS N S, et al. YersiniaBase: a genomic resource and analysis platform for comparative analysis of Yersinia[J]. BMC Bioinformatics, 2015,16:9. DOI URL PMID
[34]	SPIER S J, LEUTENEGGER C M, CARROLL S P, et al. Use of a real-time polymerase chain reaction-based fluorogenic 5' nuclease assay to evaluate insect vectors of Corynebacterium pseudotuberculosis infections in horses[J]. American Journal of Veterinary Research, 2004,65(6):829-834. URL PMID
[35]	杨焊. 四种鳞翅目害虫肠道细菌多样性分析[D]. 南京: 南京农业大学, 2012.
	YANG H. Diversity of gut bacteria in larval of four lepidopteran insect species[D]. Nanjing: Nanjing Agricultural University, 2012.(in Chinese with English abstract)
[36]	PARSHETTI G K, KALME S D, SARATALE G D. Biodegradation of malachite green by Kocuria rosea MTCC 1532[J]. Acta Chimica Slovenica, 2006,53(4):492-498.

样本名称 Sample	序列数量 Sequence number/10⁴	碱基数量 Base number/ (10⁷ bp)	平均长度 Average length/bp
BPH	3.53±0.18	1.59±0.83	449.63±0.08
SBPH	4.05±0.31	1.81±0.14	448.23±0.40
WBPH	3.87±0.39	1.72±0.17	445.66±0.20

样本名称 Sample	序列数量 Sequence number/10⁴	碱基数量 Base number/ (10⁷ bp)	平均长度 Average length/bp
BPH	3.53±0.18	1.59±0.83	449.63±0.08
SBPH	4.05±0.31	1.81±0.14	448.23±0.40
WBPH	3.87±0.39	1.72±0.17	445.66±0.20

样本 Sample	水平 Distance/%	读数 Reads	OTU	菌群丰富度 Richness		测序深度 Coverage/%	菌群多样性指数 Diversity indices
样本 Sample	水平 Distance/%	读数 Reads	OTU	Ace	Chao	测序深度 Coverage/%	Shannon	Simpson
褐飞虱BPH	3	102 952	76	78.53 (76.55,87.78)	76.50 (76.05,81.47)	99.99	1.19 (1.18,1.20)	0.49 (0.49,0.50)
灰飞虱SBPH	3	117 697	61	66.33 (62.39,81.35)	68 (62.45,94.75)	99.98	0.55 (0.54,0.56)	0.81 (0.81,0.81)
白背飞虱WBPH	3	112 189	103	103.35 (103.03,107.62)	103.00 (103,0)	99.98	2.27 (2.26,2.28)	0.19 (0.19,0.19)
褐飞虱BPH	6	104 882	49	52.67 (49.76,66.82)	50.00 (49.11,57.97)	99.99	1.12 (1.12,1.13)	0.49 (0.49,0.50)
灰飞虱SBPH	6	119 570	42	43.33 (42.18,51.90)	43.50 (42.15,57.08)	99.98	0.54 (0.53,0.55)	0.81 (0.80,0.81)
白背飞虱WBPH	6	114 492	68	68.71 (68.06,75.93)	68.00 (68.00,0)	99.98	2.21 (2.21,2.22)	0.19 (0.18,0.19)

样本 Sample	水平 Distance/%	读数 Reads	OTU	菌群丰富度 Richness		测序深度 Coverage/%	菌群多样性指数 Diversity indices
样本 Sample	水平 Distance/%	读数 Reads	OTU	Ace	Chao	测序深度 Coverage/%	Shannon	Simpson
褐飞虱BPH	3	102 952	76	78.53 (76.55,87.78)	76.50 (76.05,81.47)	99.99	1.19 (1.18,1.20)	0.49 (0.49,0.50)
灰飞虱SBPH	3	117 697	61	66.33 (62.39,81.35)	68 (62.45,94.75)	99.98	0.55 (0.54,0.56)	0.81 (0.81,0.81)
白背飞虱WBPH	3	112 189	103	103.35 (103.03,107.62)	103.00 (103,0)	99.98	2.27 (2.26,2.28)	0.19 (0.19,0.19)
褐飞虱BPH	6	104 882	49	52.67 (49.76,66.82)	50.00 (49.11,57.97)	99.99	1.12 (1.12,1.13)	0.49 (0.49,0.50)
灰飞虱SBPH	6	119 570	42	43.33 (42.18,51.90)	43.50 (42.15,57.08)	99.98	0.54 (0.53,0.55)	0.81 (0.80,0.81)
白背飞虱WBPH	6	114 492	68	68.71 (68.06,75.93)	68.00 (68.00,0)	99.98	2.21 (2.21,2.22)	0.19 (0.18,0.19)

Diversities of microbial communities in midgut of 3 planthoppers species by MiSeq

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 36

Related Articles 4

Recommended Articles

Metrics

Comments

[1]	QIAO Li, YOU Weichen, WANG Guojun, ZHI Yanan, ZHOU Zhou, GENG Shubao. Effect of different insecticides on sucking behavior of Empoasca onukii Matsuda [J]. Acta Agriculturae Zhejiangensis, 2024, 36(10): 2273-2282.
[2]	QIAO Li, CHEN Lei, JIANG Yueli, ZHOU Zhou, GENG Shubao, WANG Lijuan. Interference of LED green light on the feeding behavior of Empoasca onukii Matsuda [J]. Acta Agriculturae Zhejiangensis, 2024, 36(9): 2051-2059.
[3]	QIAO Mu, HUANG Cangyu, WU Junjing, WU Huayu, WAN Xuling, ZHOU Jiawei, LIU Guisheng, MEI Shuqi, PENG Xianwen1. Effect of porcine OLR1 gene on intramuscular preadipocytes differentiation [J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2147-2153.
[4]	YIN Haichen, LI Jianhong, WAN Peng. Effect of temperature on honeydew secretion amount and body weight of Saccharosydne procerus [J]. , 2018, 30(10): 1694-1698.