Effect of Pulsatilla saponin B4 on treatment efficiency and serum inflammatory and immune factors of dairy cows with clinical mastitis

doi:10.3969/j.issn.1004-1524.2021.07.04

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (7): 1184-1191.DOI: 10.3969/j.issn.1004-1524.2021.07.04

• Animal Science • Previous Articles Next Articles

Effect of Pulsatilla saponin B4 on treatment efficiency and serum inflammatory and immune factors of dairy cows with clinical mastitis

SHEN Liuhong¹(), QIAN Bolin¹, YOU Liuchao¹, ZHANG Yue¹, SHEN Yu¹, LYU Shangkui¹, XIAO Jinbang¹, YU Shumin¹, SU Zhetong², DONG Ke³, YANG Shilin⁴, FENG Yulin⁴^,^*(), CAO Suizhong¹^,^*()

1. Key Laboratory of Animal Disease and Human Health of Sichuan Province/The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
2. Guangxi Innovate Medical Technology Co., Ltd., Lipu 541000, China
3. Sichuan Yuqiang Materia Medica Biotechnology Co., Ltd., Chengdu 611130, China
4. National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China

Received:2020-12-10 Online:2021-07-25 Published:2021-08-06
Contact: FENG Yulin,CAO Suizhong

Abstract

Abstract:

To investigate the effects of Pulsatilla saponin B4 on therapeutic effect, serum inflammatory and immune factors in dairy cows with clinical mastitis, 12 healthy dairy cows were selected as the control group, and 36 clinical mastitis cows were selected as the test group and were randomly divided into low, middle and high dose group. Cows in test group were administered with Pulsatilla saponin B4 injection (with doses of 0.025, 0.05, 0.1 mL·kg^-1, respectively) through cervical intramuscular injection once a day for 6 days, whereas no medicine was administered in the control group. The effective and cure rates of cows in the test group were recorded, venous blood samples were collected (any day in the control group and the 1^st, 3^rd, 5^th, 7^th day in the test group) to measure serum inflammatory and immune factors, as well as milk in the infected quarters on the 1^st, 7^th, 12^th day in the test group to conduct somatic cells count (SCC) and pathogenic microorganism isolation and identification during this experiment. Cows were observed till the 12^th day and the therapeutic efficiency was recorded. It was shown that the clinical cure rate and effective rates of low, middle and high dose group were 75.00%, 75.00%, 91.67% and 100%, 100%, 100%, respectively, and the average cure time was 6.33, 6.11, 6.91 d, respectively. The Pulsatilla saponin B4 could significantly (P<0.05) attenuate SCC. Before treatment, 34 out of 36 milk samples were positive with pathogenic microorganism, indicating a detection rate of 94.44%, which mainly consisted of mycoplasma, cocci and Escherichia coli. After treatment, the total negative rate was 52.94%, among which, the negative rate of Bacilli, Staphylococcus chromogenes, Staphylococcus haemolyticus and Escherichia coli were 100%, while the negative rates of mycoplasma, Staphylococcus aureus, Staphylococcus epidermidis and coccus were 69.23%, 60.00%, 50.00% and 33.33%, respectively. Besides, Pulsatilla saponin B4 could significantly (P<0.05) promote the recovery of inflammatory factors CRP, SAA, HP, IL-1α, IL-1β, IL-2, IL-6, IL-8, TNF-α and immune factors IgA, IgD, IgE, IgG, IgM to normal levels.

Key words: Pulsatilla saponins B4, clinical mastitis, therapeutic effect, inflammatory factors, immune factors

CLC Number:

S854.5

SHEN Liuhong, QIAN Bolin, YOU Liuchao, ZHANG Yue, SHEN Yu, LYU Shangkui, XIAO Jinbang, YU Shumin, SU Zhetong, DONG Ke, YANG Shilin, FENG Yulin, CAO Suizhong. Effect of Pulsatilla saponin B4 on treatment efficiency and serum inflammatory and immune factors of dairy cows with clinical mastitis[J]. Acta Agriculturae Zhejiangensis, 2021, 33(7): 1184-1191.

Figures/Tables 5

Table 1 Influence of different dosage of Pulsatilla saponin B4 on somatic cell counts in milk of dairy cows with clinical mastitis

组别 Group	第1天 1^st day	第7天 7^th day	第12天 12^th day
B	255.8±38. $9 a a$	90.5±55. $8 ab b$	47.5±35. $6 ab b$
C	244.6±42. $0 a a$	65.8±51. $5 b b$	38.1±23. $0 b b$
D	310.6±132. $4 a a$	182.3±151. $7 a b$	48.8±58. $9 a c$

Table 1 Influence of different dosage of Pulsatilla saponin B4 on somatic cell counts in milk of dairy cows with clinical mastitis

组别 Group	第1天 1^st day	第7天 7^th day	第12天 12^th day
B	255.8±38. $9 a a$	90.5±55. $8 ab b$	47.5±35. $6 ab b$
C	244.6±42. $0 a a$	65.8±51. $5 b b$	38.1±23. $0 b b$
D	310.6±132. $4 a a$	182.3±151. $7 a b$	48.8±58. $9 a c$

Table 2 Influence of different dosage of Pulsatilla saponin B4 on cure time and cure rate of dairy cows with clinical mastitis

指标 Index	B组 Group B	C组 Group C	D组 Group D
4 d内治愈数	0	1	1
Cured number within 4 days
5 d内治愈数	2	2	2
Cured number within 5 days
6 d内治愈数	4	6	4
Cured number within 6 days
7 d内治愈数	9	8	9
Cured number within 7 days
治愈数 Cured number	9	9	11
有效数Effective number	3	3	1
无效数 Recurrence number	0	0	0
治愈率Cure rate/%	75.00	75.00	91.67
总有效率 Total effective rate/%	100	100	100
平均治愈时间	6.33	6.11	6.91
Average cure time/d

Table 3 Influence of different dosage of Pulsatilla saponins B4 on pathogenic microorganism of dairy cows with clinical mastitis

病原微生物Pathogenic microorganism	治疗前 Before treatment	治疗后 After treatment	转阴率 Negative rate%
表皮葡萄球菌 Staphylococcus epidermidis	2	1	50.00
杆菌Bacilli	1	0	100
阿尔莱特葡萄球菌 Staphylococcus arlettae	1	1	0
支原体Mycoplasma	13	4	69.23
球菌Coccus	6	4	33.33
产色葡萄球菌 Staphylococcus chromogenes	1	0	100
溶血葡萄球菌 Staphylococcus haemolyticus	1	0	100
腐生葡萄球菌Staphylococcus saprophyticus	1	1	0
大肠埃希菌 Escherichia coli	5	0	100
金黄色葡萄球菌 Staphylococcus aureus	2	2	0
葡萄球菌 Staphylococcus	3	3	0

Table 4 Influence of Pulsatilla saponin B4 on serum inflammatory factor index of dairy cows with clinical mastitis

指标 Index	对照组 Control group	试验组 Test group
指标 Index	对照组 Control group	第1天 1^st day	第3天 3^rd day	第5天 5^th day	第7天 7^th day
CRP/(mg·mL^-1)	6.55±1.68 b	8.25±2.50 a	7.02±2.08 ab	6.66±1.05 b	6.47±1.93 b
SAA/(μg·mL^-1)	6.95±1.01 c	10.86±3.20 a	9.40±2.63 ab	8.85±2.31 bc	8.71±2.60 bc
HP/(ng·mL^-1)	167.37±36.40 b	236.34±55.99 a	178.46±45.14 b	168.68±35.05 b	160.01±52.80 b
LTB4/(ng·mL^-1)	11.15±2.69 b	14.71±3.82 a	14.49±4.77 a	14.27±4.68 a	14.13±4.78 a
PGE2/(pg·mL^-1)	253.70±52.08 c	351.80±56.39 a	331.47±82.63 ab	307.83±66.35 b	299.62±51.36 b
IL-1α/(pg·mL^-1)	175.90±47.49 bc	228.47±69.62 a	210.98±63.04 ab	181.65±60.07 bc	163.23±47.55 c
IL-1β/(pg·mL^-1)	276.71±81.61 b	358.78±113.73 a	331.88±99.60 ab	325.92±98.89 ab	295.64±98.06 ab
IL-2/(pg·mL^-1)	449.65±95.09 b	606.38±130.78 a	500.38±151.23 b	470.21±145.64 b	415.76±121.10 b
IL-6/(pg·mL^-1)	97.67±22.63 c	155.00±39.96 a	134.52±38.21 ab	124.65±42.53 b	117.07±36.88 bc
IL-8/(pg·mL^-1)	108.61±38.62 b	165.45±53.22 a	153.94±50.83 a	152.60±54.40 a	139.76±47.86 ab
TNF-α/(pg·mL^-1)	122.65±33.50 c	174.65±49.07 a	151.22±41.09 ab	143.77±35.69 bc	133.20±46.61 bc

Table 5 Influence of Pulsatilla saponin B4 on serum immune factor index of dairy cows with clinical mastitis μg·mL-1

指标 Index	对照组 Control group	试验组 Test group
指标 Index	对照组 Control group	第1天 1^st day	第3天 3^rd day	第5天 5^th day	第7天 7^th day
IgA	147.58±30.60 c	239.50±38.16 a	197.57±36.29 b	196.53±40.99 b	178.71±38.18 bc
IgD	12.95±2.73 b	17.75±5.42 a	16.13±3.93 ab	15.40±4.66 ab	14.49±4.69 b
IgE	879.69±170.18 c	1 291.81±355.69 a	1 132.70±311.68 ab	1 129.13±311.64 ab	1 006.83±417.28 bc
IgG	182.37±33.29 c	275.88±74.13 a	234.38±65.60 b	229.49±54.44 b	221.81±54.44 bc
IgM	111.77±31.35 c	152.14±43.25 a	141.73±45.04 ab	137.82±34.90 ab	126.00±32.96 bc

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Effect of Pulsatilla saponin B4 on treatment efficiency and serum inflammatory and immune factors of dairy cows with clinical mastitis

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