Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (9): 1565-1580.DOI: 10.3969/j.issn.1004-1524.2021.09.01

• Crop Science • Previous Articles     Next Articles

Study on genetic diversity of sesame germplasm in Jiangxi Province based on molecular markers

WANG Zhiqi1,2(), SUN Jian2, LIANG Junchao2, ZHAO Yunyan1,2, YAN Tingxian2, YAN Xiaowen2, WEI Wenliang1,*(), LE Meiwang2,*()   

  1. 1. College of Agriculture, Yangtze University, Jingzhou 434025, China
    2. Jiangxi Province Key Laboratory of Oilcrops Biology/Nanchang Branch of National Center of Oilcrops Improvement/Crops Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
  • Received:2021-05-18 Online:2021-09-25 Published:2021-10-09
  • Contact: WEI Wenliang,LE Meiwang

Abstract:

To further evaluate the genetic diversity of 132 sesame germplasm in Jiangxi, which were collected under support of the project of “the Third National Campaign of Crop Germplasm Census and Collection”, and to clarify its genetic basis and population classification characteristics. In this study, 28 simple sequence repeat (SSR) primer pairs and 26 combinations of sequence-related amplified polymorphism (SRAP) primers were used to determine diversity and population classification among 132 sesame genotypes. The results showed that a total of 265 DNA bands were amplified by 28 SSR primer pairs, of which 221 were polymorphic bands, resulting polymorphic ratio of 83.40%, and total DNA bands and polymorphic bands amplified by each primer pair averaged 9.46 and 7.89, respectively. A total of 601 DNA bands and 268 polymorphic bands were amplified by 26 combinations of SRAP primers, resulting polymorphic ratio of 44.59%, and each primer pair amplified 9-36 DNA bands and 2-23 polymorphic bands, the polymorphic bands amplified by each primer pair averaged 10.31. Genetic similarity (GS) of 132 germplasm was between 0.598 2 and 0.960 7, with an average of 0.813 5, which showed that the overall genetic diversity was not abundant. The GS of six different geographic regions showed a gradual decline from north to south of Jiangxi Province. The genetic diversity of local germplasm in southern Jiangxi was more abundant than that in northern Jiangxi. Among different seed coat color groups, the other seed coat colors group (yellow, brown, yellowish-brown, reddish brown, etc) contained the most abundant genetic variation, followed by white group and black group. The results of cluster analysis showed that sesame accessions with similar origin tended to cluster together, indicating a correlationship between genetic similarity and geographical origin of sesame landraces in Jiangxi. Sesame germplasm resources from northern Jiangxi and eastern Jiangxi were grouped into one cluster, which revealed their closer relationship, while the remaining resources from each of the other regions were clustered into one group, which was basically consistent with the overall clustering results. The resources with different origins from three groups divided by seed color were interlaced with each other group, and their clustering results were not directly related to geographic origin. In the future collection of sesame germplasm work, we should pay attention to the utilization of southern Jiangxi germplasm and other seed-color germplasm to expand the genetic basis of sesame varieties in Jiangxi and even in China.

Key words: sesame, germplasm, genetic diversity, molecular marker, cluster analysis

CLC Number: