Effects of rhizobacteria of Cinnamomum migao H. W. Li on medicinal active ingredients in fruit and its PICRUST function predictive analysis

doi:10.3969/j.issn.1004-1524.2022.09.03

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (9): 1837-1848.DOI: 10.3969/j.issn.1004-1524.2022.09.03

• Crop Science • Previous Articles Next Articles

Effects of rhizobacteria of Cinnamomum migao H. W. Li on medicinal active ingredients in fruit and its PICRUST function predictive analysis

TIAN Xiu¹(), TONG Bingli², XIE Yuangui¹^,³^,^*(), LIAO Xiaofeng⁴, WU Tingting¹, LIU Jiming¹

1. College of Forestry, Guizhou University, Guiyang 550025, China
2. College of Life Sciences, Guizhou Normal University, Guiyang 550025, China
3. Guizhou Academy of Sciences, Guiyang 550001, China
4. Guizhou Provincial Institute of Mountain Resources, Guiyang 550001, China

Received:2021-12-17 Online:2022-09-25 Published:2022-09-30
Contact: XIE Yuangui

Abstract

Abstract:

Research on the relationship between soil rhizobacteria of authentic medicinal material Cinnamomum migao and the fruit medicinal active ingredient plays an important role on C. migao cultivation and management and yield improvement of active components. Rhizosphere soils and fruits of C. migao at the junction of Guizhou, Yunnan and Guangxi were collected. High-throughput sequencing technology was used to analyze the gene sequences of soil rhizobacteria of C. migao, soil physicochemical properties and fruit active components were determined as well. As a result, fruit active components 1,8-cineole, α-terpineol and limonene were highest in Tian’e, and cypressene was in Wangmo. Bacterial Shannon index in Wangmo was 6.315 8, higher than other sampling sites. The dominant bacterial phylum in all samples were Proteobacteria, Acidobacteria, Actinobacteria, and Chloroflexi, but it was varied in dominant genera. At the genus level, dominant bacterial genera were mainly affected by pH value and nitrogen content, most genera were positively related with active ingredients. The PICRUST functional prediction results showed that active components were mainly affected by transport and metabolism of bacterial energy and substances, indicating that pH value and nitrogen content in soil could influence the accumulation of fruit medicinal active components by changing rhizophere bacterial diversity.

Key words: Cinnamomum migao H.W.Li, rhizosphere soil, bacterial diversity, medicinal active ingredients

CLC Number:

S567.19

TIAN Xiu, TONG Bingli, XIE Yuangui, LIAO Xiaofeng, WU Tingting, LIU Jiming. Effects of rhizobacteria of Cinnamomum migao H. W. Li on medicinal active ingredients in fruit and its PICRUST function predictive analysis[J]. Acta Agriculturae Zhejiangensis, 2022, 34(9): 1837-1848.

Figures/Tables 11

Table 1 Physical and chemical properties of rhizosphere soil of C. migao

样本 Sample	pH	有机质 Organic matter/ (g·kg^-1)	全氮 Total nitrogen/ (g·kg^-1)	全钾 Total potassium/ (g·kg^-1)	全磷 Total phosphorus/ (g·kg^-1)	碱解氮 Available nitrogen/ (mg·kg^-1)	速效磷 Available phosphorus/ (mg·kg^-1)	有效钾 Available potassium/ (mg·kg^-1)
LD	4.84± 0.02 de	8.83± 0.26 a	2.29± 0.25 a	8.07± 0.15 f	0.265± 0.01 a	226.33± 7.10 c	816.07± 0.83 a	3.34± 0.12 c
WM	5.82± 0.19 c	7.51± 0.41 b	1.82± 0.11 a	32.70± 0.31b	0.175± 0.01 cde	166.83± 15.17d	819.70± 0.30 a	6.06± 0.14 a
CH	4.94± 0.02 d	6.34± 0.18 c	2.15± 0.05 a	31.27± 0.47bc	0.178± 0.01 cd	250.83± 7.65 bc	320.37± 3.43 c	3.31± 0.93 c
ZF	4.19± 0.01 f	5.92± 0.03 c	1.73± 0.19 a	23.90± 0.46d	0.150± 0.01 ef	245.00± 6.06 bc	259.67± 8.17 d	3.41± 0.03 c
TE	4.80± 0.01de	4.93± 0.63d	2.24± 0.28a	28.80± 1.36c	0.140± 0.01f	252.00± 4.04bc	803.37± 6.56a	4.31± 0.01 bc
LY	6.82± 0.01 a	8.18± 0.15 ab	2.47± 0.08 a	37.50± 0.29a	0.153± 0.01 def	268.33± 10.17b	818.80± 1.08 a	4.97± 0.11 ab
FN	5.72± 0.03 c	6.14± 0.25 c	2.61± 0.25 a	18.83± 0.23e	0.205± 0.01 b	246.17± 9.11 bc	817.60± 0.30 a	4.48± 0.10 bc
NP	4.56± 0.15 e	3.73± 0.18 e	2.24± 0.32 a	30.17± 1.34c	0.145± 0.01 f	166.83± 8.41 d	654.33± 11.62 b	5.49± 0.06 ab
LB	6.11± 0.01 b	8.19± 0.21 ab	2.73± 0.19 a	36.00± 0.30a	0.192± 0.01 bc	325.5± 2.02 a	816.70± 0.30 a	4.70± 0.04 bc

Fig.1 Content of medicinal active ingredients in C. migao fruits LD, Luodian; WM, Wangmo; CH, Ceheng; ZF, Zhenfeng; TE, Tian’e; LY, Leye; FN, Funing; NP, Napo; LB, Libo. Data on the bars marked without the same lowercase letter indicated significant differences at P<0.05. The same as below.

Table 2 Bacterial Alpha diversity of rhizosphere soil in C. migao

样本 Sample	可操作序列数 OTUs	有效序列 Valid sequences	香农指数 Shannon index	辛普森指数 Simpson index	覆盖度 Coverage/%
LY	1 199	35 789	6.052 3	0.007 3	99.19
CH	1 430	42 882	6.080 1	0.006 2	99.50
WM	1 052	30 500	6.315 8	0.003 7	99.40
LB	1 028	43 272	5.323 5	0.017 9	99.31
LD	929	42 058	5.722 1	0.008 9	99.42
ZF	1 286	33 506	5.563 6	0.009 9	99.60
TE	1 495	33 834	5.871 5	0.008 6	99.33
NP	1 315	29 592	5.599 0	0.007 7	99.64
FN	1 382	27 740	5.873 5	0.008 6	99.25

Fig.2 Distribution of relative abundance of species on phylum level

Fig.3 Distribution of relative abundance of species on class level

Fig.4 Species distribution heatmap on genus level

Fig.5 RDA analysis and correlation heatmap between rhizosphere soil characteristics and bacteria on genus level TN means total nitrogen, AN means alkaline hydrolysis nitrogen; TK means total potassium, AK means available potassium; TP means total phosphorus, AP means available phosphorus; OM means organic matter.

Fig.6 RDA analysis and correlation heatmap between medicinal active ingredients and bacteria on genus level

Fig.7 Relative abundance distribution of functional genes based on KEGG pathway

Fig.8 Relative abundance distribution of functional genes based on GOC J, Translation, ribosome structure and biogenesis; Z, Cytoskeleton; N, cell motility; L, Replication, repair and recombination; F, Nucleotide transport and metabolism; G, Carbohydrate transport and metabolism; K, Transcription ; D, Cell cycle control, cell division, chromosome partitioning; E, Amino acid transport and metabolism; S, Function unknown; C, Energy production and conversion; B, Chromatin structure and dynamics; A, RNA processing and modification; M, Cell wall/membrane/envelope biogenesis; W, Extracellular structures; Q, Secondary metabolites biosynthesis, transport, and catabolism; U, Intracellular transport, secretion, and vesicular trafficking; V, Defense mechanisms; O, Posttranslational modification, protein turnover, chaperones; H, Coenzyme transport and metabolism; P, Inorganic ion transport and metabolism; Y, Nuclear structure; I, Lipid transport and metabolism; R, General functional prediction only; T, Signal transduction mechanisms. The same as below.

Fig.9 RDA analysis of soil bacteria function in the rhizosphere and accumulation of active ingredients in the fruit

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Effects of rhizobacteria of Cinnamomum migao H. W. Li on medicinal active ingredients in fruit and its PICRUST function predictive analysis

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