Abstract: PCR was conducted with Prunus S-RNase-gene-specific primers using genome templates of Tongxiang Zui Li and 4 pollination varieties. Two target bands (about 300 bp and 500 bp, respectively) were amplified in Zui Li, only one band (about 300 bp) was amplified in each of the 4 varieties. These fragments were cloned and sequenced and two types of S-RNase-genes were identified. The longer band in Zui Li was corresponding to S-h, and the other one was corresponding to S-7. The bands in other varieties were all corresponding to S-7. There was no significant difference in the fruit setting percentage between Zui Li self\|pollination and hybridizations with related pollination varieties.

Key words: Zui Li, fertility, S genotype, pollination variety