Abstract: Based on conventional assays of mycotoxins, we have established a new rapid detection method for four kinds of mycotoxins using immune purification step and fluorescence(or HPLC) inspection technology. The new method was applied to agricultural products(cereals and oils) in the markets of Hangzhou for mycotoxins inspection and safety evaluation. There were 28 samples for total aflatoxin and aflatoxin B investigation, and the aflatoxin detection rate was about 25.0%; 48 samples were determined for ochratoxin A and the detection rate was 22.9%; 49 samples were determined for zearalenone and the detection rate was 42.9%; 39 samples were determined for deoxynivalenol and the detection rate was 15.4%. The average detection rate of the four kinds of mycotoxin was about 30%. Mycotoxin safety evaluation showed that the MRL(Maximum Residue Limit) exceeding ratio of aflatoxin and zearalenone were higher than the other two mycotoxins, and the safety level of deoxynivalenol was higher than the other three mycotoxins in Hangzhou agricultural product markets. Overall, there were widespread contaminations of cereal and oil agricultural products by mycotoxins in Hangzhou and some types of mycotoxins exceeded far beyond the MRL. Much effort should be placed to the regulation of mycotoxins safety of the cereal and oil products.

Key words: mycotoxin, rapid inspection, safety evaluation