Abstract: Rice kernile smut is a kind of common disease which is caused by Neovossia horrida which belongs to Neovossia Kornicke genera, Basidiomycete ascomycotina subdivision. The main factors of affecting teliospore germination were investigated and the medium suitable for sporulation was screened in this article so that rice kernile smut can be prevented better. We set up different parameters in this research, for example, temperature, humidity, illumination time and pH value, and observed the influence of these different parameters on the germination of teliospore so that we could find out the most appropriate conditions that the teliospore of N. horrida could germinate. The results indicated that the optimal conditions of teliospore germination of N. horrida including appropriate temperature, adequate water etc. The light and dark interval for 12 hours was favorable for the germination of teliospores. The pH value equaling to seven and adequate water were optimal conditions for the teliospore germination. Meanwhile, the morphological characteristics and germination process of N. horrida were observed and described in this article. In addition, the suitable medium of sporulation was screened in order to establish artificial inoculation system of rice kernel smut and study the interaction between N. horrida and rice. We selected seven kinds of liquid media such as bran medium and investigated the sporulation effect of them. The results showed that the bran medium was the optimal liquid medium for production of the secondary sporidia of N. horrida. We found out the optimal condition under which the teliospore of N. horrida could germinate and screened the bran medium as the sporulation medium of N. horrida.

Key words: Neovossia horrida, teliospore, germination rate, sporulation medium