Abstract: At present, many new miRNAs in Schistosoma japonicum are discovered by high\|throughout sequencing technology such as Solexa. To investigate the expression level of these miRNAs in different life stages of Schistosoma japonicum, real-time PCR was used to relatively quantify the dominantly expressed miRNAs in egg, cercarium, schistosomulum, male and female adult by combination of SYBR Green PCR and Race kit. It was found that these miRNAs were highly expressed at stages other than the egg and with different expression pattern in other stages, which were further confirmed by Northern blot. In summary, the results suggested that miNRAs might play an important role in regulation of genes transcription in different developing stages of Schistosoma japonicum．

Key words: Schistosoma japonicum, miRNAs, real time PCR