Interaction between chlorpyrifos and hepatic microsomal cytochrome P450 enzyme system in fish

›› 2011, Vol. 23 ›› Issue (4): 0-781.

• 环境科学 •

Interaction between chlorpyrifos and hepatic microsomal cytochrome P450 enzyme system in fish

BIAN Wen-jie;XU Yan;LI Shao-nan*;ZHU Guo-nian

Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, China

Received:1900-01-01 Revised:1900-01-01 Online:2011-07-25 Published:2011-07-25

Abstract

Abstract: Acute toxicity of chlorpyrifos to Danio rerio, Pseudorasbora parva, and Lepomis gibbosus, oxidative activation of chlorpyrifos catalyzed by hepatic microsomal cytochrome P450 system, and influence of chlorpyrifos to the P450 system were studied to evaluate the risk of chlorpyrifos on ecology system. 96 h LC50 of chlorpyrifos to the Danio rerio, the Pseudorasbora parva, and the Lepomis gibbosus was measured to be 1.94, 0.0273, 0.0681 mg·L-1, respectively. The Vmax/Km of chlorpyrifos oxidation catalyzed by P450 system was measured to be 1.67×10⁴,5.00×10⁴,2.00×10⁴,respectively, indicating that the oxidative metabolism capacity of the P450 system was the highest in Pseudorasbora parva and the lowest in Danio rerio, which matched the results of acute toxicity tests. The influence of chlorpyrifos to the P450 system was not obvious．

Key words: chlorpyrifos, hepatic microsomal, cytochrome P450s, fish

BIAN Wen-jie;XU Yan;LI Shao-nan*;ZHU Guo-nian. Interaction between chlorpyrifos and hepatic microsomal cytochrome P450 enzyme system in fish[J]. , 2011, 23(4): 0-781.

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Interaction between chlorpyrifos and hepatic microsomal cytochrome P450 enzyme system in fish

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