Abstract: Efficient microbial strains and appropriate reaction conditions are the keys to the biotransformation of cellulose. Based on cellulosedecomposing microorganisms isolated, we studied synergism of cellulosedecomposing microorganisms and properties of crude enzyme liquid. Results showed that synergism was significant between the degrading bacterium, enzyme activity that X2 and X5 mixed culture produced was up to 066 U·mL-1, which increased nearly 1 times than that of singledegrading bacteria. The optimum temperature and pH of crude enzyme were 25℃ and 50 respectively. The inhibitory effect of EDTA on the enzyme activity was more obvious. Ca2+ and Mn2+ were activators of the crude enzyme solution, which could significantly enhance the enzyme activity in the concentration range of the study. The results provided important germplasm resources and technical parameters for the bioconversion of cellulose.

Key words: cellulosedecomposing microorganisms, synergism, metal ions