Abstract: A quantitative real-time PCR assay was established for detection of Tomato yellow leaf curl virus. The primers and probe were designed according to the CP gene sequence of Tomato yellow leaf curl virus.
Results indicated that the standard curve showed the linear relationship between Ct(cycle threshold) and template concentration, and the regression coefficient of the quantitative curve was 0.9941. The sensitivity of the assay was 1 000 plasmid copies and it was 100 fold sensitive than conventional PCR. The specificity of the assay was high and there was no cross reactions with ToMV and CMV. The real-time PCR system we established is rapid, sensitive and steady to detect TYLCV．

Key words: Tomato yellow leaf curl virus(TYLCV), quantitative real time PCR, TaqMan probe, quantitative detection