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    24 September 2012, Volume 24 Issue 5 Previous Issue    Next Issue

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    Effects of plastic film covering on improving illumination and fruit quality of citrus variety Bendizao
    WU Shao-hui;SHI Xue-gen;*;CHEN Jun-wei;*;WANG Peng
    2012, 24(5):  0-829. 
    Abstract ( 866 )   PDF (1174KB) ( 1222 )  
    Three different kinds of plastic films were used in orange orchard of citrus variety Bendizao. Light reflection of the lower part of tree crown and fruit quality were detected. Results showed that light reflection ratio of orange orchard was increased when cover by plastic film. Color change of orange peel was promoted, especially the fruits in the lower part under three plastic films. Soluble solids content, total sugar content, vitamin C content, reducing sugar content and sucrose content were increased under three plastic films compared to the control group. According to this paper, Japanese film and tyveck mulching has shown more effective on improving the fruit quality of Bendizao.
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    Effect of drought stress on physiological characteristics of sorghum at seedling stage
    XIE Fang;ZHAI Guo-wei;ZOU Gui-hua;TAO Yue-zhi;*
    2012, 24(5):  0-757. 
    Abstract ( 601 )   PDF (1206KB) ( 1205 )  
    A stay-green line (RIL-1) and an early-senescence line (RIL-2) were selected from a recombinant inbred line population of 654/LTR108. Five physiological indices were investigated from the leaf samples collected from the seedlings of RIL-1, RIL-2, 654 and LTR108 under drought stress, respectively. The level of proline content increased significantly with the prolonging of drought stress. RIL-2 had the highest level of proline content, followed by LTR108, 654 and RIL-1. The chlorophyll content of 654 and RIL-1 firstly increased and then decreased while RIL-2 and LTR108 exhibited a down-up-down changing pattern. The level of T-AOC activity followed an up-down pattern for all the lines investigated. RIL-2 and LTR108 had the highest level of T-AOC activities at the 3rd day, while RIL-1 and 654 reached the highest level at the 4th day. T-SOD activity had a similar pattern as T-AOC activity. The significant change was observed for RIL-1. Different patterns were observed for the level of the POD activity among 654, RIL-1, RIL-2 and LTR108, up-down pattern for 654, RIL-1 and RIL-2 but down-up-down pattern for LTR108, respectively. The significant change was occurred for the line of 654.
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    PolyA trapping by using terminatorless RFP reporter gene in rice
    LI Jun;QYU Shao-hong*
    2012, 24(5):  0-763. 
    Abstract ( 637 )   PDF (1340KB) ( 1182 )  
    Gene trapping method has been widely used in plant functional genomic research. PolyA trapping vector pLJ19 which contains GFP and RFP reporter genes was constructed, GFP reporter gene was used for detecting transformation events and RFP reporter gene was used for PolyA trapping. Calli both with GFP and RFP fluorescence were selected and T-DNA insertion sites were analysed by Genome walking PCR. The plasmid pLJ19 was introduced into calli of Oryza sativa L. ssp. Japonica cv. Nipponbare and then 17 transgenic calli both with GFP and RFP. FST of T-DNA insertion sites indicated that 6 T-DNA were inserted into rice genome and 5 of 6 them were inserted around rice gene 3’ ends. These results indicated that the plasmid pLJ19 was available in PolyA trapping.
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    Polymorphisms detection of intron 20 of Chinese Holstein STAT4 gene using PCR-RFLP method
    ZHANG Lei;SONG Xue-mei;JIANG Jun-fang;JIANG Yong-qing*
    2012, 24(5):  0-781. 
    Abstract ( 561 )   PDF (1496KB) ( 1015 )  
    This study was aimed to establish a simple detective method for polymorphisms of Chinese Holstein STAT4 gene intron 20. Three hundred and fifty-seven Chinese Holstein cows were used in this study. Firstly, the polymorphism of intron 20 of STAT4 gene was detected by PCR-SSCP method combined with DNA sequencing analysis. Then the detective method was transformed from PCR-SSCP to PCR-RFLP according to DNA sequencing results of the mutant SSCP patterns. The results showed that intron 20 of STAT4 gene had a complete linkage haplotype block (g.95879 G>A and g.96013 G>C.A). SduⅠ enzyme detective system was established according to the characteristic that the 96013 G→C mutation may be formed as a restriction enzyme SduⅠ polymorphism. By this detective system, three different genotypes, GGGG, GAGC and AACC were found, and the corresponding genotype frequencies were 0.0784, 0.4538 and 0.4678. The frequencies of alleles GG and AC were 0.3053 and 0.6947, respectively.
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    Cloning and expression of E gene of duck Tembusu virus and preliminary application
    YANG Shao-yan;YU Ke-xiang;WANG Hua;SHI Yu-ying;MA Xiu-li;LI Jian-liang;CUI Yan-shun;*
    2012, 24(5):  0-786. 
    Abstract ( 645 )   PDF (1405KB) ( 1176 )  
    The whole cDNA of E gene was amplified by PCR from duck Tembusu virus (DTMUV) strain BZ, and cloned into the pMD18-T vector. The fragment was identified by restriction enzymes digestion with EcoRⅠ and XhoⅠ, and then was cloned into the pET-28a (+) vector. The recombinant expression plasmid PET28a-E was obtained and transformed into BL21 (DE3). After the recombinant bacteria were induced by optimal concentration of IPTG, the E fusion protein was proved to get exact expression and have good immunogenicity by SDS-PAGE and Western blotting. Base on the expressed protein an indirect ELISA was established to detect DTMUV antibody in duck serum. The indirect ELISA shared 95.0% coincidence rate with the neutralization test, which showed that this method had a good prospect on the detection of DTMUV antibody.
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    Evaluation of drought resistance on the rice germplasms introducted from Myanmar at the seedling stage
    DU Jia-hui;DONG Chao;TANG Cui-feng;XYU Fu-rong;ZHANG Fei-fei;YANG Ya-yun;ZHANG En-lai;A Xing-xiang;DAI Lu-yuan;*
    2012, 24(5):  0-770. 
    Abstract ( 665 )   PDF (1127KB) ( 1063 )  
    To evaluate the drought-resistance of 160 varieties introduced from Myanmar at seedling stage, Hanyou No.3, Hanyou No.8 were used as drought-resistant varieties but Zhenshan 97B as drought-sensitive variety in this study, respectively. Five drought-resistance indexes including survival percentage under repeated drought conditions, plant height, length of the fist leaf under central leaf, relative electrolyte leakage and malondialdehyde content under repeated drought conditions at seedling stage have been measured. Comprehensive appraisal index (comprehensive D values) was the five index of fuzzy subordinate-function(FSV) average. The drought resistance of resources introduced from Myanmar was classified into 4 levels of resistance, moderate resistance, moderate sensitivity, sensitivity accroding to D values of the control. And 18 materials with drought-tolerance were screened, which indicated the 18 materials can be used as breeding resources to improve the drought resistance of cultivated rice.
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    Dry matter and main nutrient accumulation and distribution of oriental tobacco during growth in Zhejiang
    WANG De-hua;FU Yun-peng;*;HUANG Ruo-ping;MENG Fei;YU Xing-zhen;OU Ya-fei;ZHANG Guo
    2012, 24(5):  0-776. 
    Abstract ( 546 )   PDF (1139KB) ( 1003 )  
    The accumulation and distribution rules of dry matter and main nutrients of oriental tobacco were studied in Zhejiang. The results showed that the peak period of dry matter accumulation in leaves and stem of Samsun and in leaves of Canik was during 35-65 days after transplanting, while that in stem of Canik was during 30-80 days after transplanting. Also, the distribution ratio of dry matter in leaves and stem was 32.18% and 53.98% in Samsun, and 24.74% and 58.78% in Canik, respectively. In addition, the absorption level of N, K and S in Samsun and the absorption level of N, P, K, Ca and Mg in Canik reached the peak during 35-65 days after transplanting, while the absorption level of P, Ca, Mg and Cl in Samsun and the absorption level of S and Cl in Canik reached the peak during 35-80 days after transplanting. Furthermore, during the growing season, the order of accumulation of major nutrients from high to low was K>Ca>N>S>Cl>Mg>P in Samsun, and was K>N>Ca>S>Cl>Mg>P in Canik. The accumulation of K, P, Ca, Mg, S in Samsun was higher than that in Canik, while the accumulation of N, Cl in Samsun was lower than that in Canik.
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    Bioinformatics analysis on common carp Homeobox A2b gene
    GENG Bo;SUN Xiao-wen
    2012, 24(5):  0-791. 
    Abstract ( 599 )   PDF (3363KB) ( 1028 )  
    Hox gene family is a set of key genes of embryo development and one of the important models to study gene duplication and chromosome doubling during biological evolution. In this paper, by using comparative genomics and bioinformatics approach, we extracted HoxA2b gene sequence from the carp genome database and accomplished sequence analysis and comparison, and drew four kinds of homologous evolutionary tree. As a result, it is found that HoxA2b gene is very conservative and the homology of most of Cyprinidae HoxA2b genes is between 99%-100%. We found a conserved sequence whose length is 188 bp, encoding 62 amino acids. The homology in the conserved region of carp HoxA2b gene with other 20 organisms is about 85%-100% and the relative genetic distance is between 1.14-1.55. By analysis the rate of base substitution, we found that the nonsynonymous nucleotide substitution rate is much higher than synonymous nucleotide substitution rate. The carp HoxA2b gene has greater genetic variation and has far genetic distance with other organisms which may be related to carp genome replication and chromosome doubling.
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    Preparation of clenbuterol-imprinted material using surface-molecular imprinting method and its molecule recognition character
    YANG Ting;CHEN Guo;WU Yin-liang;ZHAO Jian;ZHU Yong
    2012, 24(5):  0-796. 
    Abstract ( 620 )   PDF (1237KB) ( 1218 )  
    To synthesize the molecular imprinted polymer (MIP) for clenbuterol and study the recognition property of the MIP, polymethylacrylic acid was grafted onto the surface of silica gel particles via the coupling effect of γ-Methacryloxypropyl trimethoxy silane, and the grafting particle was prepared. Then, the molecular imprinting towards the grafted particle was performed with clenbuterol as template molecule and ethylene glycol diglycidylether as crosslinking agent by using the surface-molecular imprinting technique established, and the clenbuterol molecule-imprinted material was prepared. The morphology of MIP was observed by scanning electron microscope (SEM), and the chemical structure of MIP was characterized by Fourier transform infrared spectroscopy (FT-IR). Static method was adopted to study the binding properties and molecule recognition character of MIP for clenbuterol. The experiment results showed that MIP had specific recognition selectivity, excellent binding affinity and elution property for clenbuterol.
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    Canonical correlation analysis on some of the growth and development traits of Bihu pigs
    CHEN Hai-yan;
    2012, 24(5):  0-800. 
    Abstract ( 484 )   PDF (1086KB) ( 1089 )  
    The canonical correlation analysis on 4 weight traits and 9 body size traits of Bihu pig showed that: the first and second canonical correlation coefficients between weight traits and body size traits was highly significant. The value was 0.9980 and 0.9933 respectively, which represented 98.95% for the total correlation.
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    Identification of marine fungal strain NJ0104 with the anti-microbial activities of their metabolites to bacterial pathogen of Pseudosciaena
    LIU Hui-hui;LI Shu-ping;ZHAO Qian;ZHAO Shu-jiang;*
    2012, 24(5):  0-807. 
    Abstract ( 631 )   PDF (1844KB) ( 1112 )  
    A marine fungal strain NJ0104, isolated from marine sediments near Nanji island sea, Wenzhou, Zhejiang, could produce active metabolites against bacterial pathogen of Pseudosciaena. To identify this strain, morphological and ITS-58S rDNA regions of the strain was amplified through PCR reaction and sequenced. Mycelium showed a polybranch morphology, there was no nucleus, but septum were present in the mycelium and produced conidiospores. The length of ITS sequence was 1027 bp. The results of morphological observation and genetic relationship of strain NJ0104 was close to Arthinium and the similarity was more than 98%. Based upon the combination of phenotypic and genotypic data, the strain was identified as Arthrinium sp.
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    Identification of heat tolerance in cucumber
    CHE Jiang-yu;ZHOU Sheng-mao;SHANG Xiao-hong;*;MI Jun-hong;LIU Wen-jun;LIANG Ren-fan;WEN Jun-li;HUANG Ru-kui
    2012, 24(5):  0-813. 
    Abstract ( 531 )   PDF (1441KB) ( 1140 )  
    In order to obtain highly heat\|tolerant cucumber germplasms for the breeding of heat-tolerant cucumber varieties, thirty-eight cucumber varieties from different sources were employed to determinate both germination percentages of seeds at the germination stage and the indexes of heat injuries at the seedling stage at more than 40℃. And then they were comprehensively evaluated by membership function in the aspect of heat tolerance. The results showed that heat-tolerance of cucumber at germination stage was basically consistent to that at the seedling stage. Three highly heat-tolerant cucumber germplasms and 11 heat-sensitive ones were obtained on the basis of the above evaluations. The aforesaid results suggested that heat-tolerance of cucumber germplasms could be evaluated at both seed germination stage and seedling stage, and the heat-tolerant cucumber germplasms obtained would be the substantial materials for the breeding of heat-tolerant cucumber varieties.
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    Effects of vapor-permeable reflective film mulching on fruit quality of peach
    HU Xiang-ming;ZHANG Hui-qin;XIAO Jin-ping;ZHANG Chen;MA Chang-nian;XIE Ming;*
    2012, 24(5):  0-820. 
    Abstract ( 506 )   PDF (1274KB) ( 1092 )  
    Effects of vapor-permeable reflective film mulching on sugar accumulation, organic acid content and bioactivator (total phenolics, anthocyanin contents) of two peach varieties were investigated, meanwhile, the antioxidant capacity was measured by assays of 1,1′-diphenyl-2-picrylhydraz scavenging(DPPH) and Ferric reducing antioxidant power(FRAP). The results showed that mulching of vapor-permeable reflective film significantly increased the total soluble solid content (TSS), reduced the titratable acid content (TA), and increased the total phenol content and antioxidant capacity. The correlation analysis showed that there was significantly positive correlation between the total sugar content and the sucrose content, as well as TA content and quinic acid and isocitric acid. This indicated that the increase of TSS under mulching of vapor-permeable reflective film was caused by the increase of sucrose content, and the decrease of TA content was attributed to the reduction of quinic acid and isocitric acid content. The total phenol content was significantly positive correlated with the fructose content, DPPH free radical scavenging capacity and iron ion reductive ability. It was concluded that the increased fructose content promoted the synthesis of phenolics and increased the antioxidant capacity of fruits; furthermore, the increased anthocyanin contents in the peel improved fruit coloring and increased fruit brightness, the L* and a* values were higher that the control under mulching of vapor-permeable reflective film.
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    Effects of natural grass growing on the soil fertility and fruit quality of Myrica rubra
    LIANG Sen-miao;WANG Qin-hong;NI Guo-fu;QIAN Li-ping;ZHAO Xian-sheng;QI Xing-jiang;*
    2012, 24(5):  0-825. 
    Abstract ( 565 )   PDF (1111KB) ( 1143 )  
    Natural grass growing in Myrica rubra orchard is a good way to improve the soil fertility and the fruit quality of Myrica rubra. Our studies indicated that natural grass growing can increase plant diversity of Myrica rubra orchard, improve soil fertility, increase single fruit weight, single plant yield and the ratio of good quality fruit significantly. The results also showed that the total fruit sugar content and the total soluble solid content were the highest with half natural grass growing method, being 10.82% and 12.12%, respectively. The natural grass growing enriched plant diversity, improved soil fertility and fruit quality. The results offered a reference for safety production of Myrica rubra.
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    Microclimate characteristics and its effect on yield and quality of tea green in plastic greenhouse tea garden in Nanjing Region
    YIN Jian-mei;ZHANG Pei-tong;HAN Xiao-yong;PENG Qi;GUO Wen-qi;ZHANG Heng-you
    2012, 24(5):  0-835. 
    Abstract ( 589 )   PDF (1122KB) ( 1046 )  
    In order to study the microclimate characteristics of plastic greenhouse tea garden and its effect on yield and quality of tea green, experiments were carried out with three modes of tea garden which were normal tea garden, plastic greenhouse tea garden with insect prevention net and plastic greenhouse tea garden with insect prevention net and shade cloths, to determine the ecological environment factors of tea gardens (including the illumination, relative humidity, temperature and soil moisture content), yield and biochemical indicators of tea green (including amino acid, theine, tea polyphenol, chlorophyll) in constant two years in Qiaoli Tea Garden, Gaochun, Nanjing. The results showed that the temperature were comparatively stabile in plastic greenhouse tea gardens, but the illumination was lower and the yield of tea green increased. The amino acids and chlorophyll contents of tea green in spring, summer and autumn were increased, but the theine and tea polyphenol contents were decreased in spring tea green and increased in summer and autumn tea green. The plastic greenhouse with shade cloths can improve tea quality, especially, the yield and quality of summer and autumn tea, but was unfavorable to the tea green yield of spring and before Tomb-sweeping Day. So the plastic greenhouse should be covered without shade cloths in spring and with it in summer and autumn.
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    Analysis of genetic diversity of tea landrace resources in Guizhou by EST-SSR markers
    NIU Su-zhen;LIU Yu-qian;YANG Jin-biao;FAN Wei-guo;*
    2012, 24(5):  0-841. 
    Abstract ( 484 )   PDF (1179KB) ( 1215 )  
    Twenty-five tea landrace from Guizhou province, as well as three developed cultivar tea, were analyzed by 29 EST-SSR markers. A total of 104 alleles were identified with an average of 3.58 alleles per marker. The average of effective number of alleles, observed heterozygosity, expected heterozygosity and polymorphism information content were 2.85, 0.56, 0.66 and 0.58, respectively. The Qianbei tea germplasms showed lower values of the five indexes mentioned above, compared to the Qiannan Region. The Fis, Fit, Fst and gene flow were 0.04, 0.12, 0.08, and 3.01, respectively. The population structure analysis showed the genetic similarity coefficient ranged from 0.39 to 0.89,could divide all the 28 accessions into 4 groups. Tea germplasms were grouped into groupⅠ,Ⅱ,Ⅲ,Ⅳ, including 4,5,8,11 tea germplasms, respectively. It was suggested that the materials used in the experiment possessed a broad genetic differences and a higher genetic diversity. The results objectively reflect information including population structure and regional differences. The genetic diversity of tea germplasms in the Qianbei Region was lower than that of Qiannan Region. The tea germplasms in Qianbei Region showed richer genetic diversity than that in Qiannan Region, with relatively high genetic divergence and weak gene flow.
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    Detection of Tomato yellow leaf curl virus by real-time PCR with TaqMan probe
    RUAN Mei-ying;YE Qing-jing;WANG Rong-qing;ZHOU Guo-zhi;YAO Zhu-ping;LI Zhi-miao;WAN Hong-jian;YANG Yue-jian*
    2012, 24(5):  0-845. 
    Abstract ( 593 )   PDF (1377KB) ( 1195 )  
    A quantitative real-time PCR assay was established for detection of Tomato yellow leaf curl virus. The primers and probe were designed according to the CP gene sequence of Tomato yellow leaf curl virus.
    Results indicated that the standard curve showed the linear relationship between Ct(cycle threshold) and template concentration, and the regression coefficient of the quantitative curve was 0.9941. The sensitivity of the assay was 1 000 plasmid copies and it was 100 fold sensitive than conventional PCR. The specificity of the assay was high and there was no cross reactions with ToMV and CMV. The real-time PCR system we established is rapid, sensitive and steady to detect TYLCV.
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    Heavy metal pollution assessment of farmland soil influenced by e-waste disassembling
    ZHOU Cui;YANG Xiang-tian;HE Xian-biao
    2012, 24(5):  0-890. 
    Abstract ( 567 )   PDF (1199KB) ( 1259 )  
    An assessment was carried on the heavy metal concentrations in the farmland soil around e-waste disassembling areas, and the different evaluation methods and standards were employed to evaluate the soil quality. The results showed that Cd and Cu were the main pollutants in the farmland soil by the assessment of single factor pollution indexes based on the level of National Standard (GB15612-1995). Comprehensive pollution index indicated that over 50% of the soil was above the level of moderate pollution, while about 20% soils were heavily polluted. However, based on the level natural background, the soil was mainly polluted by Cd and Pb by the assessment of single factor pollution indexes. Comprehensive pollution index indicated that over 80% of the soils were above the level of moderate pollution, and about 40% soils were heavily polluted.
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    Construction of a genetic linkage map of a bacterial blight resistance rice line derived from Oryza meyeriana L.
    YANG Yong;CHEN Li-na;YAN Cheng-qi;CHENG Ye;CHENG Xiao-yue;CHEN Jian-ping;*
    2012, 24(5):  0-852. 
    Abstract ( 529 )   PDF (1885KB) ( 1073 )  
    Y73, a progeny line from asymmetric somatic hybridization between wild rice species Oryza meyeriana L. and cultivated rice O. sativa L., combines high bacterial blight (BB) resistance of the wild rice with good agronomic characters of the cultivated rice. In this study, a genetic linkage map of Y73 was constructed based on a recombinant inbred line (RIL) population derived from a cross between Y73 and a BB susceptible cultivar IR24. The map was consisted of 155 simple sequence repeats (SSR) markers and 56 sequence-tagged site (STS) markers, covering about 1 5400 cM of the rice genome with an average distance between adjacent markers of 7.89 cM. The genetic linkage map will facilitate the mapping of quantitative trait loci (QTLs) and the isolation of important genes for BB resistance and other agronomic traits, and then will be a valuable resource for marker-assisted breeding.
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    The acute toxicity and risk assessment of 25 pesticides used in nectar plant to Apis mellifera L.
    CANG Tao;WANG Yan-hua;YU Rui-xian;WU Chang-xing;CHEN Li-ping;WU Sheng-gan;ZHAO Xue-ping*
    2012, 24(5):  0-859. 
    Abstract ( 983 )   PDF (1127KB) ( 1656 )  
    Twenty-five pesticides, which were commonly used in the period of flowering of 4 main nectar plants in Zhejiang province, were chosen to determine the acute toxicity to bees (Apis mellifera L.) combining with the field maximum dose. The results showed that: the 48 h LC50 values of 1.8% EC abamectin to Apis mellifera L. was 0.0528 (0.0462-0.0603) mg·L-1, belongs to very high toxic grade; the 48 h LC50 values range of 1% EC emamectin benzoate, 40% EC methidathion, 10% AS nitenpyram, 40% EC phoxim, 2.5% EC lambda-cyhalothrin, 4.5% EC beta\|cypermethrin, 480 g·L-1 EC chlorpyrifos, 45% EC malathion, 10% WP imidacloprid and 90% TC trichlorfon to Apis mellifera L. were 1.02 (0.869-1.14) mg·L-1 to 13.9 (12.4-15.5) mg·L-1, belongs to high toxic grade; the 48 h LC50 values range of 15% EC pyridaben, 20% SP acetamiprid and 15% WP ethylicin to Apis mellifera L. were 43.3(37.5-50.0) mg·L-1 to 163(127-210) mg·L-1, belongs to moderate toxic grade; the LC50 values of 5% SC fenpyroximate, 250 g·L-1 EC prochloraz, 25% WP diflubenzuron, 40% EC propargite, 50% WP cyromazine, 50% WG dimethomorph, 75% WG carbendazim, 25% WP buprofezin, 35% SC procymidone, 50% WP iprodione and 75% WP chlorothalonil to Apis mellifera L. were higher than 200 mg·L-1, belongs to low toxic grade. The results showed that 1.8% EC abamectin, 1% EC emamectin benzoate, 40% EC methidathion, 10% AS nitenpyram, 40% EC phoxim, 2.5% EC lambda\|cyhalothrin, 4.5% EC beta\|cypermethrin, 480 g·L-1 EC chlorpyrifos, 45% EC malathion and 90% TC trichlorfon had very high risk to Apis mellifera L. 10% WP imidacloprid, 15% EC pyridaben and 5% SC fenpyroximate had high risk to Apis mellifera L. 20% SP acetamiprid, 15% WP ethylicin, 250 g·L-1 EC prochloraz, 40% EC propargite, 50% WP cyromazine and 50% WG dimethomorph were moderately risk to Apis mellifera L., while other pesticides were low-risk to Apis mellifera L.
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    Residue analysis and degradation dynamics of fenoxanil in rice
    ZHANG Chang-peng;ZHAO Hua;WU Min;ZHANG Chun-rong;HU Xiu-qing;PING Li-feng;LI Zhen*
    2012, 24(5):  0-864. 
    Abstract ( 690 )   PDF (1135KB) ( 1100 )  
    The method for the determination of fenoxanil residues in paddy plant, soil and water were estimated for evaluating the safety of fenoxanil in paddy field. The samples were extracted with ethyl acetate, cleaned-up using a Florisil solid phase extraction cartridge, and subsequently determinate by gas chromatography-electron capture detector(GC-ECD). Average recoveries of fenoxanil in paddy plant, soil and water were found in the range of 74.98%-102.96% at the three spiking levels from 0.05 mg·kg-1 to 5.00 mg·kg-1 with relative standard deviations of 0.68%-8.07%. The limits of quantification (LOQ) in paddy plant, soil and water were 0.05 mg·kg-1. The field experiments demonstrated that the decline curves of fenoxanil in paddy field were accorded with the first-order kinetics equation. The half-lives of fenoxanil in paddy plant, soil and water were 3.62-5.51, 3.60-24.32, 2.29-26.66 d, respectively.Fenoxanil is easily degradable pesticide in paddy plant, soil and water.
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    Quantity effects analysis of Bt genes on the endophytic prokaryotes in transgenic rice
    JIANG Nan;WANG Xiao-fu;XYU Jun-feng;HUANG Zhi-hong;ZHOU Yu;*
    2012, 24(5):  0-874. 
    Abstract ( 488 )   PDF (1304KB) ( 1012 )  
    The communities (quantity effects) of endophytic prokaryotes in transgenic and wild-type rice were investigated in the present study. At first, the optimal sterilizing methods for the surface of leaves, stems and roots of rice were investigated and determined as follows: rice roots and the other tissues (stems and leaves) could be sterilized through soaking in 70% ethanol for 7 min and 2 min, respectively; both ethanol-soaking were followed by washing with distilled water twice. Thereafter, the number of endophytic prokaryotes in the roots, stems and leaves of rice was examined, and the results showed that the number of endophytic prokaryotes in all parts of transgenic rice was less than that of wild-type rice at the seedling stage; the similar results were also observed at the stooling stage, except that in the leaves of transgenic rice TT51-1. The number of endophytic prokaryotes in all parts of rice at stooling stage was higher than that at seedling stage for both transgenic and wild\|type rice. Our data showed that Bt gene insertion could significantly affect on the number of endophytic prokaryotes of rices in both of seedling and stooling stages.
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    Population dynamics of white\|backed planthopper and its predatory spiders in hybrid rice fields with different nitrogen regimes
    ZHOU Xiao-jun;XYU Hong-xing;ZHENG Xyu-song;YANG Ya-jun;CHEN Li-wei;HE Jin-hao;LYU Zhong-xian;*
    2012, 24(5):  0-869. 
    Abstract ( 575 )   PDF (1094KB) ( 1107 )  
    Effects of nitrogen regimes on the population dynamics of white-backed planthopper (WBPH), Sogatella furcifera, and its predatory spiders on hybrid rice were determined in single season rice fields in Jinhua, China. The results showed that there was a similar tendency in WBPH population dynamics in the three tested hybrid rice fields with different nitrogen regimes, and the peak of population was found at the rice elongating stage. The abundance of WPBH population was significantly increased with the elevated application of nitrogen fertilizer, while the highest abundance of WBPH population was recorded during rice elongating and booting stages in the rice field of Shenliangyou 5814. Application of nitrogen fertilizer could obviously increase the number of predatory spiders before full panicle stage in all three tested rice fields. Three-way ANOVA indicated that the population dynamics of WBPH and spiders were significantly affected by nitrogen regime, hybrid varieties and their growth stages.
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    Evaluation of land intensive use in development zones of Gansu province based on coordination degree model
    ZHANG Li-ping;LIU Xue-lu*;MA Xiao-jing
    2012, 24(5):  0-897. 
    Abstract ( 468 )   PDF (1106KB) ( 1099 )  
    Taking 36 city development zones in Gansu province as an example, through the establishment of evaluation index system, this article took a quantitative analysis on the evaluation of land intensive use in development zones on the basis of variation coefficient method with determined weight. The results showed that it was possible to evaluate the coordination degree of intensive land use zone system and the subsystems, and it can identify the specific indicators and its actual level of uncoordinated system according to the efficiency values of single indicator. The coordination degree of three types of intensified land use was quite different. The coordination degree of the land intensive use of the high-tech industrial park was the highest, and the economic development zone and special industrial zone were relatively lower. The development context and evaluation scope of parts of the development zones was inconsistent, which caused that the evaluation results could not reflect the true situation of the development zones, so it should be considered to adjust the scope of parts of the development zones.
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    Screening and identification of an antagonistic Burkholderia pyrrocinia strain A12 and its growth\|promoting effects on tobacco seedling
    HAN Chao;WU Gui-yuan;LIU Ai-xin*;WANG Yu-jun*
    2012, 24(5):  0-885. 
    Abstract ( 558 )   PDF (1395KB) ( 1141 )  
    An antagonistic bacterium strain A12 has strong antimicrobial activity to many plant pathogens. By morphological, physiological and biochemical assays, and 16S rDNA and recA gene sequence homology analysis, A12 was identified as Burkholderia pyrrocinia. Antifungal spectrum was confirmed by confrontation tests, the results showed that A12 has significant inhibitory effect against Rhizoctonia solani, Fusarium graminearum, Verticillium dahliae, etc. In greenhouse tests, B. pyrrocinia A12 showed promoting effects on tobacco chlorophyll content, photosynthesis rate, transpiration rate and root growth.
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    Studies on the damage and control index of Sclerotinia stem rot of rape under mechanical direct seeding condition
    ZHONG Lie-quan;WANG Hui-fu;*;WANG En-guo;LI Ke-cai;MA Chi-fang;SHI Tong-wu
    2012, 24(5):  0-879. 
    Abstract ( 526 )   PDF (1117KB) ( 1052 )  
    Real-time monitoring and investigation showed that Sclerotinia stem rot of mechanical direct seeding rape occurred earlier than common sort. Disease severity rose with the rape developed, and more quickly during the early stage of anthesis, flower withering period and early silique mature period. Yield loss rate increased with the ill stem rate and disease index enhanced. Yield loss rate(Y1) correlated with ill stem rate(X1), as well as disease index(X2), all reached a highly significant level with related model Y1=0.5443302X1-2.3161(n=14,r=0.9860**), Y1=0.673408 X2-1.0164(n=14,r=0.9772**). Disease control index of mechanical direct seeding rape was ill stem rate as 11.8% and disease index as 7.6 when controlled once, respectively, and ill stem rate as 16.8% and disease index as 11.7 when controlled twice.
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    Analytical analysis of paddy runoff disturbance coefficient
    JIAO Rui-feng;ZHANG Hong-rui;BIAN Xin-min;*
    2012, 24(5):  0-903. 
    Abstract ( 490 )   PDF (1127KB) ( 1077 )  
    In order to investigate the effects of leaf area index (LAI) and rainfall interception (RI) on paddy total nitrogen(TN) and total phosphorus(TP) concentration in rainfall runoff process, simulated rainfall experiments were conducted by setting leaf area index (LAI) as 2, 4, 6, with rice in early transplanting stage, late tillering stage, booting stage, and treatment without leaf covering, and setting rainfall interception (RI) as 5.0,12.5,25.0,50.0 mm · h-1. Disturbance coefficient was used to express the effects of LAI and RI on paddy runoff total TN and TP concentration. The results showed that LAI and RI had significant effect on disturbance coefficient. Based on analytic analysis and coupling, analytical equation of TN and TP was respectively given as fallows: =0.070×ln(RI)-0.035×LAI+0.618(R2=0.905) and =0.002×RI-0.048×LAI+0.867(R2=0.913).
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    Effects of different plastic\|film mulching on soil properties as well as maturity period and fruit quality of precocious plum in a plum orchard
    LIU Shu-xin;DING Feng-hua;ZHU Wei-qing;PAN Zhi-mei;*
    2012, 24(5):  0-908. 
    Abstract ( 591 )   PDF (1120KB) ( 1124 )  
    A field experiment was conducted to determine the effects of different plastic-film mulching on soil properties as well as maturity period and fruit quality of precocious plum in a 6-year-plum orchard. The results showed that ordinary light film mulching significantly increased soil surface temperature, whereas reflective film mulching markedly increased soil temperature in the low temperature season and reduced the soil surface temperature in the high temperature season compared with the bare ground. Plastic-film mulching could increase the soil water content, soil permeable capability as well as soil organic matter, N, P, K and other nutrient contents, but decreased soil bulk density. Ordinary light film mulching had set flower bud break and florescence one week ahead. Reflective film mulching had set fruit picking 7 d and 3 d ahead, respectively, compared with ordinary light film mulching and the bare ground. Improvement in fruit sugar, soluble solids, and protein and vitamin C contents in the fruits followed the order as: reflective film mulching>ordinary light film>the bare ground.
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    Effects of different pretreatment methods on the enzymatic hydrolysis of rapeseed protein
    WANG Wei-xing;CHEN Jian-bing;YANG Ying;ZHENG Mei-yu;LU Sheng-min;*
    2012, 24(5):  0-913. 
    Abstract ( 535 )   PDF (1148KB) ( 1028 )  
    In this study, rapeseed protein solution (40 mg·mL-1) was pretreated with different heat (60, 80 and 100℃), ultrasonic power(200, 300 and 500 W), sodium triphosphate and succinic anhydride respectively before being hydrolyzed by alcalase for 3 hours. Peptide content and DPPH radical scavenging activity in hydrolysates were investigated. Results indicted that the best pretreatment alone was 60℃, 500 W, sodium triphosphate with an amount of three times of protein weight, and succinic anhydride in a dosage of 0.05 times of protein weight. Under these pretreatments, peptide contents were 35.00%±0.18%,41.48%±0.66%,39.27%±0.74% and 40.39%±0.34% respectively. The DPPH radical scavenging activities of the four modified protein hydrolysis solutions were all higher than the control.
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    Prediction study of tomato dry weight based on BP neural network
    WANG Li-yan;GUO Shu-guo
    2012, 24(5):  0-925. 
    Abstract ( 528 )   PDF (1262KB) ( 1226 )  
    Orthogonal experimental was carried out using the tomato dry weight as experimental objective, and the impact of environmental parameters (temperature, relative humidity, light intensity) on tomato dry weight was analyzed through experiment. A neural network calculation model was established based on experimental data and the test were made by using MATLAB software, and to better verify effectiveness of the approach, a 10-fold cross validation method was used. Through 10-fold cross validation, model achieved the predictive accuracy of 95.32%. The results showed that the predicted values were in good agreement with the experimental values. This method has high prediction precision, which can be used theory instruction in environment control, and the predicted dry weight of tomato with the BP neural network method was feasible.
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    SYBR Green I real-time polymerase chain reaction for rapid detection of Salmonella spp. in raw milk and milk products
    ZHANG Qiao-yan;*;CHEN Ting-ting;CHEN Xiao-yun;YANG Sheng-li;*;MIU Qing-mei
    2012, 24(5):  0-921. 
    Abstract ( 635 )   PDF (1819KB) ( 1205 )  
    Raw milk was susceptible to Salmonella spp., which was reproduced rapidly. Nevertheless, there was only the potential of very small amount of Salmonella spp. in milk products since sterilization and other treatments though the bacterial population decreased. In this paper, we studied the pretreatment methods of raw milk and milk products according to their contamination levels and optimized the extraction conditions of bacterial total DNA by the boiling lysis procedure. Furthermore, we set up a systemic detection technology for milk-borne Salmonella spp., based on SYBR Green I real\|time PCR targeting the invA gene. The developed methods possessed specificity for Salmonella spp. As far as raw milk was concerned, the limit of detection of the method was 102 cfu·mL-1, the linear range of the standard curve was 102-108 cfu·mL-1, correlation coefficient(R2) was 0.999, and amplification efficiency was 103%. For milk products, 1 cfu of Salmonella spp. present in 25 g (mL) milk samples was shown detectable by this method with an enrichment step of 16 h. This technology could be used to quantitatively detect Salmonella spp. in raw milk only for 3 h, and qualitatively assay milk products only for 1 d. All in all, our work provided a high\|throughout detection technology of Salmonella spp. for dairy enterprises, which was proved rapid, simple, sensitive and accurate with a good reproducibility
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    Classification of fruit based on the BP neural network
    YAO Li-jian;BIAN Qi;LEI Liang-yu;ZHAO Da-xu
    2012, 24(5):  0-930. 
    Abstract ( 564 )   PDF (1534KB) ( 1140 )  
    This paper introduced one classification method for fruit grade based on BP neural network. Fruit images were preprocessed by using digital image processing method. The mean color value and variance of fruit surface were selected to express fruit color features. An ellipse that has the same normalized second central moments with the fruit region were adopted to approximately represent fruit shape. It simplified the complex degree of the shape description. The optimum structure parameters of the BP neural network which had 9 hidden layer neurons were determined by RP training algorithm. Results showed that average accuracy for fruit classification can reach 92.5% by using this model, and the executing time of microcomputer for grading of one apple is 10.3 ms. This method has the characteristics of high accuracy and good real-time performance.
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    Study on vertical development model of agricultural tourism in Poyang Lake basin
    JIN Shu-lan;YAO Ning-ping;PAN Hua-hua
    2012, 24(5):  0-935. 
    Abstract ( 548 )   PDF (1162KB) ( 1135 )  
    Agricultural tourism exploitation is an effective way to balance urban and rural development. According to characteristics of agricultural tourism, natural landscape, resources and environment of Poyang Lake basin and the interactional relationship between target market and spatial structure in system, it is necessary to construct the sightseeing zones of migrants, experiencing zones of fishing in waters and wetland, farming culture zones in plain, leisure agriculture tourism areas of characteristic planting and breeding from basins to hills and complex agricultural tourist areas with the function of vocation, cognition, sightseeing and leisure on mountains. The measures of vertical agricultural tourism areas can realize harmonious developments among urban and rural management, industrial convergence, economy and ecology in Poyang Lake basin.
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    Quantitative analysis and the evaluation of agricultural leading industry in Henan
    WANG Liang;CHEN Shou-yue;ZHU Li-qun;BIAN Xin-min*
    2012, 24(5):  0-942. 
    Abstract ( 551 )   PDF (1114KB) ( 1098 )  
    Correct choice of the leading industry of agriculture is the main way to fully exploit its advantages in resources and labor, optimize the industrial structure and promote the regional characteristic agricultural economy. Based on the present situation of agricultural leading industry in Henan Province, we analyzed the industrial advantages of crop farming, livestock breeding and the fruit industry using the method of aggregated advantage index and the location entropy. Furthermore, each leading industry of agriculture was selected out and the countermeasures for promoting the development of agricultural leading industry in Henan Province was proposed combined with the regional natural resource, location condition and agricultural economy present situation.
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    Review on application and advances of nuclear techniques in agriculture in Zhejiang province
    LU Yan-ting;XIONG Li-dong;WU Qing;JIN Qing-sheng*
    2012, 24(5):  0-948. 
    Abstract ( 496 )   PDF (1119KB) ( 1147 )  
    This paper reviewed the development and main achievements of nuclear agricultural sciences in Zhejiang province in the past 50 years, including radiation mutation breeding, space mutation breeding and related heredity mechanism, radiation sterile insect technique and related molecular biology mechanism, the agricultural products storage and preservation by irradiation processing, isotope tracing techniques for animal-plant physiology, biochemical and agrochemical safety. The possible development and trend of nuclear agroscience was discussed and the modern bio-technology and infotechnology was suggested to be introduced for improving its methodology.
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