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Effects of seed priming with different agents on seed germination and seedling growth in tobacco (Nicotiana tabacum L) under drought stress MA Wen-guang;CUI Hua-wei;LI Yong-ping;ZHENG Yun-ye;WANG Yang;QIN Guo-chen;HU Jin;* 2012, 24(6):  0-956.  Abstract ( 879 )   PDF (1274KB) ( 1344 )   The effects of different agents on seed germination and growth characteristics of tobacco varieties with different drought tolerance were studied in this paper Seeds of two tobacco varieties with different drought tolerance were primed in different concentration solutions of paclobutrazol， calcium chloride， proline and salicylic acid Then， the germination， seedling growth and physiological changes under drought stress (simulating with 15% polyethylene glycol 6000) were measured The results showed that the germination and seedling growth were inhibited， however， the activities of ascorbate peroxidase (APX)， catalase (CAT)， peroxidase (POD) and superoxide dismutase (SOD) in tobacco seedlings were enhanced and the malondialdehyde (MDA) concentration were decreased after treated with paclobutrazol (PP333) The germination parameters， root length， seedling length， seedling fresh and dry weight were improved， the activities of APX， CAT， POD and SOD were enhanced and the MDA concentration were decreased in two tobacco varieties after treated with calcium chloride， proline and salicylic acid The treatment of 80 mg·L-1 salicylic acid had the best effects among all treatments， suggesting that this treatment can be used to improve drought tolerance of tobacco seeds and seedlings. Related Articles | Metrics

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Primary research on corn haploid breeding LYU Gui-hua;XYU Xiu;LU Hua-bing;GUO Guo-jin* 2012, 24(6):  0-960.  Abstract ( 611 )   PDF (1143KB) ( 1491 )   Eight different genotype female parent materials were induced by three haploid inducers （Stock 6， Gaoyou 1 and MT041） to test their ability of producing haploid through grain color， chromosome number of root tip， stomatal density and field planting performance， and colchicine was used for doubling. The results showed that haploid induction rates of different inducers differed greatly. The haploid induction rate of MT041 was 4.17%， which was higher than the other two inducers. The female genotypes showed significant difference in induction rates. The haploid induction rates of the waxy and ordinary materials were significantly higher than sweet materials. The sweetwaxy corn hybrids were beneficial to increase the sweet corn haploids. The doubling effect of 0.06% colchicine solution was significantly higher than 0.02% colchicine solution by rootsoaking， while seedling percentage in high concentration colchicine treatment was not high. Chromosome doubling is still a bottleneck restricting widespread application of the DH breeding techniques． Related Articles | Metrics

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Application of Mutation Surveyor in analyzing heterozygous InDel SONG Xuemei;JIANG Junfang;JIANG Yongqing* 2012, 24(6):  0-968.  Abstract ( 647 )   PDF (3305KB) ( 1186 )   The present study aimed to show the application of Mutation Surveyor in analyzing nucleotide substitutions and InDel with an instance. The polymorphisms in ISG15 5′ flanking sequences were analyzed for 144 Hu sheep from Yuhang Hu sheep farm and slaughterhouse by polymerase chain reaction\|single strand conformation polymorphism （PCR\|SSCP） technique， DNA sequencing and Mutation Surveyor software. PCR\|SSCP analysis showed ISG15 5′ flanking sequences bands were too complex to determine genotype directly. The analysis result of Mutation Surveyor found there were three polymorphisms， 152 C/T， 158 dupC and 239 C/T， in ISG15 5 ′flanking region. The frequency of allele C in loci 152 was 083 showing low polymorphism （PIC = 0.2424）. The frequency of allele dupC in loci 158 was 05125 showing mediate polymorphism （PIC = 0.3748）. The frequency of allele C in loci 239 was 07675 showing mediate polymorphism （PIC = 0.2932）. Except 239 C/T， the gene frequencies and genotype frequencies of Hu sheep 152 C/T and 158 dupC tested have reached the Hardy\|Weinberg equilibrium （P>0.05）. Mutation Surveyor software has greater advantage in analysis of nucleotide substitutions， especially InDel sequencing data. So Mutation Surveyor software can be used for SNPs high\|throughput screening and analysis． Related Articles | Metrics

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Effect of different rooting agents on cutting propagation of Huiming baicha LI Yang;YAN Fang;HE Weizhong*;BAO Huansheng 2012, 24(6):  0-991.  Abstract ( 456 )   PDF (1191KB) ( 1234 )   In order to screen out suitable kinds and concentrations of rooting agents， six different rooting agents were used to investigate their effects on rooting and growth of cutting propagation in Huiming baicha. The results showed that all rooting agents had significant effect under suitable concentration， especially the treatment of 800 mg·L-1 ABT1 solution had the best effect on the index of wound healing rate， nodulation rate， rooting rate， survival rate， number of roots， average length and weight of root for cutting seedlings， and the survival rate was 89.5%. The new shoot grew best and the length was 25.3 cm in the treatment of mixed solution of 400 mg·L-1 IBA + NAA （2:1）， which had better effect than the single treatment of IBA or NAA. Related Articles | Metrics

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Associations between immune traits and genetic variation of Mx gene in Shandong indigenous chicken populations WANG Shibo;LEI Qiuxia;LI Fuwei;HAN Haixia;ZHOU Yan;LI Guiming;CAO Dingguo;*;SHAN Hu;* 2012, 24(6):  0-974.  Abstract ( 588 )   PDF (1354KB) ( 1079 )   The PCRSSCP was used to study the associations between immune traits(sheep red blood cell, avian influenza and newcastle disease)and polymorphic loci of the Mx gene in Shandong indigenous chicken populations(Luqin chicken, Shi qiza chicken, Langya chicken, Laiwu black chicken and Jining Bairi chicken). The fragment of Mx gene about 150 bp including intron 13 and exon 14 was amplified. The results showed that two single nucleotide polymorphisms were detected in the five indigenous chicken populations by PCRSSCP: 1 892 points of coding sequence of Mx gene G→A (Ser→Asn); 1 911 points G→A, which couldnt lead to amino acid mutation. Through the analysis on the association between the polymorphic loci of the exon 14 and the antibody response, the SNPs on the 1 892 points of coding sequence of Mx gene in Luqin chicken, Langya chicken and Laiwu black chicken was correlated to antibody response of H9 significantly(P＜0.05). Related Articles | Metrics

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Studies on BMPRIB， BMP15 and GDF9 as candidate genes of fecundity in four breeds of goats in Fujian LI Wenyang;LIU Yuan;LIN Shixin;ZHANG Xiaopei;GAO Chengfang;LIN Bifen;DONG Xiaoning 2012, 24(6):  0-979.  Abstract ( 469 )   PDF (1435KB) ( 990 )   Our current project was conducted by using BMPRIB， BMP15 and GDF9 as candidate genes to study the relationship among polymorphisms of BMPRIB， BMP15 and GDF9 and the fecundity in sheep and goat. PCRRFLP was applied to analyze the relationship between the gene and genotype polymorphisms of BMPRIB， BMP15 and GDF9 in four Fujian goat breeds and their prolific trait. The results showed that there was no same mutation in BMPRIB gene of four goat breeds as that of Booroola Merino. The FecXI， FecXH， FecXB of BMP15 and FecGH of GDF9 were not found. The possibility that the fecundity of four goat breeds were affected by the mutations of BMPRIB， BMP15 and GDF9 gene could be ruled out. As we dont know the whole gene sequence of the three genes in four goat breeds， its hard to determine whether these genes affect the fecundity of them. Related Articles | Metrics

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Effect of saccharicterpenin on the quality of boar sperm in high temperature season CHU XiaoHong;HU JinPing;XYU Ruhai;LU Fuzeng;DAI Lihe;JIANG Hongjin 2012, 24(6):  0-982.  Abstract ( 460 )   PDF (1192KB) ( 1099 )   This study was conducted to study the effect of saccharicterpenin on the quality of boar sperm in high temperature season (from July to September， 2011) in Zhejiang province， adding 400 g·t-1 saccharicterpenin in a diet of boar The result indicated that there was an increase in semen volume， density， motility and pH value， but no significant difference (P＞0.05) Meanwhile， there was significant increase (P＜0.05) in testosterone level In summary， saccharicterpenin can increase the quality of boar sperm in high temperature season. Related Articles | Metrics

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Aroma compounds extracted from Tieguanyin tea by various methods and its application performance in cigarette filters MA Tao;CAO Qiue;HE Xuefeng;WEN Guanghe;*;LIU Runchang;SHEN Yan 2012, 24(6):  0-987.  Abstract ( 480 )   PDF (1147KB) ( 1065 )   To get a better understanding of aroma compounds derived from tea， extracts from Tieguanyin tea were obtained by the methods of water extraction， ethanol extraction， supercritical carbon dioxide extraction， and subcritical extraction， respectively， of which the volatile and semi\|volatile compounds， especially aroma compounds， were determined by the combination of gas chromatography and mass spectrum method （GC\|MS）. Additionally， performances of the various extracts on cigarette quality were investigated when the extracts were applied in cigarette filters as well. As the results indicated， compared with the other three methods， supercritical carbon dioxide extraction showed a better contribution to aroma compounds extraction from Tieguanyin tea in this research. According to the assessment of cigarette quality taste， extracts from supercritical carbon dioxide extraction method also had a better significant influence on it. It is concluded that supercritical carbon dioxide extraction is a relatively appropriate method， with high efficiency and economic effectiveness， for preparing cigarette quality-oriented extract． Related Articles | Metrics

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Effect of exogenous nitric oxide and GGR6 on cormel development of several plants of Lycoris YIN Dandan;XUE Zeyun;XYU Yingchun;*;LI Yongrong;ZHAI Min;ZHOU Jie 2012, 24(6):  0-997.  Abstract ( 542 )   PDF (1170KB) ( 995 )   The Lycoris bulbs were treated with eight division method of cutting. Effects of different concentrations of nitric oxide （NO） and GGR6 powder on cormel development in five native species of Lycoris were investigated. The results were as follows: The cormel number， cormel diameter， cormel height， cormel fresh weight， root number and root length of the five genus Lycoris were different， meanwhile each index showed different response to experimental treatments. The cormel formation number of L.chinensis and L. rosea was promoted by relatively higher concentration of NO， whose optimal concentration range was 50-75 μmol·L-1. Relatively higher concentration of GGR6 powder was good for cormel development of L. sprengeri and L. albiflora， whose optimal concentration range was 50-75 mg·L-1. The cormel quality of L. radiate was effectively increased by 25 μmol·L-1 NO according to the index of cormel diameter， cormel height and cormel fresh weight. Related Articles | Metrics

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Effect of different types of bagging treatment on fruit quality of pear LI Xianming;QIN Zhongqi*;TU Junfan;YANG Fuchen;ZHU Hongyan;WU Tao 2012, 24(6):  0-1003.  Abstract ( 449 )   PDF (1161KB) ( 1267 )   Pyrus pyrifolia Nakai. cv. Eli No.2 was used as material to study the effect of different types of bagging on fruit quality. The results showed that bagging could significantly improve the appearance of fruits with smaller and less fruit dots， and decrease rusty stain index. There was significant linear relation between transparency of bags（x） and the size of fruits（y） with the regression equation of YW=159.61+0.99x. Regression equation and correlation coefficients showed as: brightness （L）， YL=74.45-0.71x; colority coordinate value （A）， YA=-16.68-0.77x; colority coordinate value （B）， YB=52.52+1.86x. The Vc content was higher than that of the control. The SSC/TA and SS/TA of the fruits in bagging treatment increased compared to the controls. According to the results， it was concluded that the most appropriate paper bag types for P.pyrifolia Nakai. cv. Eli 2 were twolayer bags from Xiaolin， South korea， Taiguo and twice bagging was commendatory in the manufacturing of P.pyrifolia Nakai cv. Eli No.2. Related Articles | Metrics

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Study on sucrose accumulation and enzyme activities involved in sucrose metabolism in developing seeds of vegetable soybean ZHANG Guwen;HU Qizan;XYU Shengchun;GONG Yaming* 2012, 24(6):  0-1020.  Abstract ( 584 )   PDF (1208KB) ( 1150 )   Three cultivars， Zhenong No.6 （high sucrose content）， Liaoxian No.1 （middle sucrose content） and Xiafeng 2008 （low sucrose content） were used to investigate the sucrose accumulation and enzyme activities involved in its metabolism in developing seeds of vegetable soybean. The results showed that the growth curves of vegetable soybean seeds showed single sigmoid pattern. The change trends of sucrose metabolism among three cultivars were similar. During the whole periods of vegetable soybean seeds development， sucrose was the major component of soluble sugar， taking about 70% of total soluble sugar contents. The net activities of sucrose\|metabolizing enzymes were the major factor affecting sucrose accumulation. During the early stage of seed development， net activities of sucrose\|metabolizing enzymes were negative value. Sucrose metabolism is in the decomposition direction， when sucrose synthase and acid invertase were key enzymes in sucrose decomposition. However， the net activities of sucrose\|metabolizing enzymes turned to positive value in the middle stage of seed development. Sucrose metabolism was in the synthesis direction， when sucrose synthase and sucrose phosphate synthase were key enzymes in sucrose synthesis. During the late stage of seed development， also the net activities of sucrose\|metabolizing enzymes were positive value， but its value was close to zero， showing that the rate of sucrose decomposition and synthesis was similar and sucrose synthase， sucrose phosphate synthase， acid invertase and neutral invertase played a leading role in sucrose metabolism in this stage． Related Articles | Metrics

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Characteristics of fruit quality component accumulation in developing fruits of three white flesh loquat germplasm resources in Lishui ZHOU Xiaoyin;CHEN Junwei;*;XYU Hongxia;QI Xingjiang;LI Guobin 2012, 24(6):  0-1008.  Abstract ( 503 )   PDF (2474KB) ( 1043 )   The dynamics of fresh weight， color parameter， sugar， organic acid， and total phenolics and flavonoids contents were examined in three white fleshy loquat (Eriobotrya japonica (Thunb.)Lindl) resources The results showed the change trends in fruit weight and color development were similar among four white fleshy loquats The biggest fruit size was Ninghaibai among the four varieties， the size of Big Taipingbai was close to Ninghaibai， Taipingbai was the smallest one The change trends of sugars， organic acids， total phenolics and flavonoids contents were also similar The fructose was the major sugar of these four white fleshy loquat varieties， which accounted for 54%-61% of total sugar content The glucose took the second content component of sugars， which accounted for 35%-37% of total sugar content The highest sugar content was in fruit of Taxiabai， Taipinbai ranked the second， and the lowest sugar content was in fruit of Big Taipinbai The titratable acids and organic acids contents were the highest in fruit of Taxiabai， the content in Taipinbai was the second， Big Taipingbai was the lowest The contents of total phenolics and flavonoids were relatively high in the early stage of fruit development， thereafter quickly declined with fruit development， kept constant in final two weeks before maturation The difference in total phenolics and flavonoids content among four varieties was little The key stage of fruit quality components accumulation began from three weeks before maturation， when the loquat fruit grew quickly， sugars accumulated fast， and organic acid declined rapidly. Related Articles | Metrics

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Morphological and molecular study of Ulva in Xiangshan， Fenghua and Gouqi Island， Zhejiang GAO Song;MA Jiahai*;CAI Yongchao;JYU Min;CHEN Binbin;ZENG Yanping;HE Peimin 2012, 24(6):  0-1014.  Abstract ( 460 )   PDF (2589KB) ( 997 )   Green algae Ulva grow widely along the coastline of China Zhejiang Province is a major province which makes use of Ulva Its products not only supply the domestic but also the international market Common Ulva samples were collected from different locations in different times in 2011， including inner bay of Xiangshan， Fenghua and open sea island Gouqi Identification was conducted by combining morphological observation and molecular biological methods The results showed that dominant species in Xiangshan and Fenghua from January to March was Ulva prolifera， cohabitating with Ulva linza and Ulva flexuosa However， in Gouqi Island Ulva pertusa and Ulva fasciata were more common. Related Articles | Metrics

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Morphological and molecular study of Ulva in Xiangshan， Fenghua and Gouqi Island， Zhejiang GAO Song;MA Jiahai*;CAI Yongchao;JYU Min;CHEN Binbin;ZENG Yanping;HE Peimin 2012, 24(6):  0-1014.  Abstract ( 427 )   PDF (2589KB) ( 1008 )   Green algae Ulva grow widely along the coastline of China Zhejiang Province is a major province which makes use of Ulva Its products not only supply the domestic but also the international market Common Ulva samples were collected from different locations in different times in 2011， including inner bay of Xiangshan， Fenghua and open sea island Gouqi Identification was conducted by combining morphological observation and molecular biological methods The results showed that dominant species in Xiangshan and Fenghua from January to March was Ulva prolifera， cohabitating with Ulva linza and Ulva flexuosa However， in Gouqi Island Ulva pertusa and Ulva fasciata were more common. Related Articles | Metrics

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AFLP analysis of genetic diversity of Angelica dahurica SHEN Xiaoxia;SHEN Yufeng;JIANG Jianming 2012, 24(6):  0-1025.  Abstract ( 521 )   PDF (1413KB) ( 1018 )   The 50 germplasmic resources of Angelica dahurica were analyzed by AFLP to determine the genetic diversity. The amplified fragment length polymorphism （AFLP） tags were used to investigate the genetic diversity at the DNA level. Twelve pairs of selective amplification primers were screened out to amplify the bands， which were used as original matrix and analyzed the similarity between different Angelica dahurica germplasm by NTSYS\|PC software calculation and constructed the phylogenetic tree. The results indicated that the similarity of the 50 Angelica dahurica germplasm resources all more than 70%， whose overall phylogenetic relationships were relatively close. The germplasm ZYS002039 and ZYS002045 showed larger difference in genetic background from the others． Related Articles | Metrics

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Evaluation on compatibility of interspecific cross between several roses, Old Blush and modern roses WANG Zefan;HAN Qian;LIN Yiyan;CUI Jiaopeng;LIU Hengxing;ZHAO Shiwei;ZHAO Huien;* 2012, 24(6):  0-1032.  Abstract ( 583 )   PDF (1618KB) ( 1195 )   The hybridization experiment was carried out using Rosa primula, Rosa xanthina f. spontanea, Rosa chinensis cv. Old Blush and Rosa laxa as male parents, while modern roses in the fields as female parents. According to early breeding work, there were 22 hybridizations with affinity as follows: Eyeopener× Rosa primula, Berk Shire× Rosa xanthina f. spontanea, Sally Holmes× Rosa xanthina f. spontanea, Partidge× Rosa xanthina f. spontanea, Partidge× Rosa primula, Partidge×Old Blush, Partidge× Rosa laxa, Cambridgeshire× Rosa xanthina f. spontanea, Robin Redbreast× Rosa primula,Summer Sunrise× Rosa primula,Tess of The Durbervilles× Rosa primula, Petal Pushers× Rosa primula, Laura Ford ×Rosa xanthina f. spontanea,Jill Dando ×Rosa xanthina f. spontanea,Jill Dando ×Rosa primula,Twenty\|fifth ×Rosa primula,Phea Sant ×Rosa primula,Grouse ×Rosa xanthina f. spontanea,Grouse×Rosa primula,Summer Sunset× Rosa xanthina f. spontanea,Anna Zinkeisen×Rosa xanthina f. spontanea,Anna Zinkeisen× Rosa laxa． Related Articles | Metrics

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Investigation and classification of Chimonanthus praecox cultivars in central and eastern Henan LU Jianguo;WANG Jianmei*;RONG Juan 2012, 24(6):  0-1039.  Abstract ( 500 )   PDF (1230KB) ( 1004 )   According to the flower characters，the cultivars of Chimonanthus praecox were investigated and classified in central and eastern Henan， China. The results showed that 156 cultivars in Henan province were divided into three groups according to the purple speckles of inner tepals including Ch.praecox Concolor Group， Ch.praecox Intermedius Group and Ch.praecox Patens Group and identification key of the cultivars has been compiled. Related Articles | Metrics

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Comparison of the RNA isolation methods for Pleurotus ferulae Lenzi hyphostroma WU Zhiyan;YE Jingyun;CHENG Shan;YAN Xunyou* 2012, 24(6):  0-1044.  Abstract ( 460 )   PDF (1273KB) ( 1005 )   Total RNA of Pleurotus ferulae Lenzi was extracted by single-step method, STE method and guanidinium isothiocyanate method, whose isolation effects were compared. The results showed that it was difficult to extract total RNA from Pleurotus ferulae Lenzi by single\|step method. Total RNA could be extracted by guanidinium isothiocyanate method, but RNA extracted was immingled by some protein and was not enough for molecular biology experiment. Single\|step method and guanidiniu isothiocyanate method were not suitable for RNA extraction from Pleurotus ferulae Lenzi because of its high amylase content. Total RNA extracted by STE method had high quality, which is the best method of total RNA isolation from Pleurotus ferulae Lenzi. The quality of total RNA was analyzed with agarose gel electrophoresis and nucleic acid and ultraviolet spectrophotometer. Three bright bands were observed in agarose gel electrophoresis as 28S, 18S and 5S. The band of 28S was twice wider than that of 18S, and the value of OD260/OD280 was 18 to 20.These results demonstrated that the quality and purity of RNA obtained by the method of STE could meet the demands of molecular biology experiment． Related Articles | Metrics

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Cloning and prokaryotic expression of Cymbidium mosaic virus TGB1 and TGB2 gene DENGYanfu;CHEN Zhijuan;CHENG Xiaofei;ZHANG Pengcheng;HU Feng;SHI Nongnong* 2012, 24(6):  0-1049.  Abstract ( 520 )   PDF (1312KB) ( 1049 )   Cymbidium mosaic virus (CymMV) is an important virus infecting Orchidaceous plants severely In this study， according to the genomic sequence of CymMV in GenBank (Accession No AB197937)， the specific primers were designed for amplifying its TGB1 and TGB2 gene， respectively By reverse transcriptionpolymerase chain reaction (RTPCR)， the TGB1 and TGB2 genes were amplified from CymMVinfected Phalaenopsis leaves Both fragments were then inserted into pGEX4T3 vector for prokaryotic expression The constructed expression vectors were transformed into E coli BL21 that was induced by IPTG for expressing the proteins SDSPAGE and Western blot analysis confirmed that the expressed proteins were GSTfused CymMV TGB1 and TGB2 proteins Expression of TGB1 and TGB2 will help to prepare the antibodies for their further functional analysis. Related Articles | Metrics

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Study on the occurrence regularity and prediction model of underground pest adults in Hangzhou district WANG Daoze;HONG Wenying;WU Yanjun;WANG Aijuan;WEI Jiqian 2012, 24(6):  0-1057.  Abstract ( 516 )   PDF (1361KB) ( 1041 )   For improving forecasting and sustained control damage level， light trap numbers and field dynamic of underground pests (black cutworm， mole cricket and scarab beetle) in Hangzhou district during the years of 2005 to 2011 were investigated The results showed that different pests had obvious differences in population dynamic The black cutworm， Agrotis ypsilon， had several damage peaks (late May， late June and late July etc)， and the moth amount of the prophase was relatively high The mole cricket， Gryllotalpa africana， had two damage peaks (late May to early July， early September to middlelate October) The scarab beetle， Anomala corpulenta， had one damage peak (late May to late June) There were periodic changes in total numbers of underground insect pests among years， and the peak period was the year of 2005， 2007 to 2009 and 2011， respectively On this basis， temperature， humidity， precipitation， illumination etc were used as forecasting factors， using the method of stepwise regression， nineteen factors with significant correlation were screened， and occurrence amount forecasting models and occurrence period forecasting models of the three insects were established By using accuracy degree judge model for verification， the score values of occurrence amount and occurrence period forecasting models of the three underground pests were more than 58 and 70， which indicated that the historical coincident rate and prediction accuracy of established forecasting models were good. Related Articles | Metrics

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Biocontrol potential of Caenorhabditis sp CNM strain discovered from the compost of Agaricus bisporus LI Xingyue;LIU Qizhi*;YANG Daowei 2012, 24(6):  0-1063.  Abstract ( 507 )   PDF (1349KB) ( 988 )   One species of insect nematode (Caenorhabditis sp CNM)， which can cause Galleria mellonella death within 4 days， was discovered in the compost of Agaricus bisporus through the Galleria bait method In order to discover the biologicalcontrol potential of CNM in the field application， we investigated its biology characteristics， especially the infectivity and antistress resistance The result of its infectivity to G mellonella showed that the larger of the dosage， the better of its infectivity LD50 of CNM to G mellonella was 46.6 IJs per larva， and the LC50 is 53.8 IJs·cm-2 at 48 h After 45 min， 20 h and 55 h， all of the nematodes died with water bathing of 42℃， 40℃ and 38℃ Therere still 46.0% of the nematodes alive after 12 h under 25℃， 50.5 % RH， and the infectivity decrease was 43.3% Besides， there were re 57.1% of nematode that can move for as far as 3.6 cm under 30℃ So Caenorhabditis sp CNM strain has the biocontrol potential with good infectivity， heat resistance and desiccation. Related Articles | Metrics

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Pathogen identification of anthracnose of Photinia serrulata and lab screening of fungicides ZHANG Huili;XYU Ying;WANG Jiangling;LI Yuxiang;GU Jianfeng 2012, 24(6):  0-1068.  Abstract ( 560 )   PDF (1283KB) ( 1044 )   Colletotrichum sp isolated from Photinia serrulata was identified as Colletotrichum gloeosporioides based on its morphological and molecular characteristics The antifungal activities of 14 fungicides against C gloeosporioides were determined by mycelium growth rate method Four of these fungicides were primarily screened， and their toxic effects on Cgloeosporioides were tested， respectively The results showed that 25％ Prochloraz EC exhibited significant inhibition activity at concentration of 000004 μg·mL-1，then is Prochloraz EC (450 g·L-1) at concentration of 0.00005 μg·mL-1，Prochloraz EW (450 g·L-1) at concentration of 0.00018 μg·mL-1， and 50% prochlorazmanganese WP at concentraion of 0.2554 μg·mL-1. Related Articles | Metrics

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Authentication of Kuznets curve on agricultural nonpoint source pollution in Zhejiang Province ZHAO Liange;XYU Jianfen;WANG Xueyuan 2012, 24(6):  0-1085.  Abstract ( 456 )   PDF (1186KB) ( 1082 )   Based on the data of agricultural nonpoint source pollution emissions and economic development indicators from 1995 to 2010 in Zhejiang Province， we used EKC model to verify whether the relationship between Zhejiang agricultural nonpoint source pollution and economic development showed inverted Ushape The results showed that the curve which represents the relationship between Zhejiang agricultural nonpoint source pollution emissions and the real GDP per capita was a typical inverted Ushape curve At present， it is the turning period of Zhejiang agricultural nonpoint source pollution and the pollution emission will reduce as the economy developed However， since EKC is just a description of the future trend of environmental quality， not a forecast There are still many problems in EKC theory， such as econometric model problem， metrics problem and so on Thus its wrong to believe the EKC hypothesis without thinking Environmental quality will not improve automatically； the agricultural environment of Zhejiang province is still facing triple pressure of rapid economic growth， environmental quality improvement and environmental risk prevention We can improve environment by carrying out economic and environmental policy， improving peoples quality and using technology. Related Articles | Metrics

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Feeding preference of Cnaphalocrocis medinalis larvae on artificial diets with different nitrogen and sugar contents GUO Wenqing;YANG Yajun;*;XYU Hongxing;ZHENG Xyusong;LYU Zhongxian;* 2012, 24(6):  0-1073.  Abstract ( 537 )   PDF (1227KB) ( 1074 )   Cnaphalocrocis medinalis is an important rice insect pest in China， whose mass rearing in laboratory is too difficult to limit its research This study reported the feeding preference of C medinalis larvae to various artificial diets containing different contents of nitrogen and soluble sugar The results showed that newlyhatched larvae preferred artificial diet with higher sugar Nitrogen content in diet showed no significant effect on the selectivity of newlyhatched larvae in total，but the selectivity was affected by nitrogen at 2 h after infestation and the proportion of newlyhatched larvae on the diet with 0.92% nitrogen was 36% in total； and after injection for 48 h the number of larvae on diet with 1.16% nitrogen was higher than others Sugar content could affect the selectivity of 3rd instar larvae Nitrogen content also affected the numbers of 3rd instar larvae on the different diets， and 3rd instar larvae preferred diet with 1.53% nitrogen The 5th instar larvae liked to choose diet with high sugar (27.22%) The amount of nitrogen showed no obvious effect on choosing different nitrogen artificial diets of 5th instar larvae In total， soluble sugar plays an important role in the feeding preference of C medinalis larvae， while nitrogen only affects the activity of 3rdinstar larvae in feeding preference. Related Articles | Metrics

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Prevention and control technology of pollution in industrial tissue culture FANG Li;WANG Yiting;LYU Yongping;Kumar;CHEN Jianping;WANG Hanrong;* 2012, 24(6):  0-1078.  Abstract ( 610 )   PDF (1144KB) ( 1085 )   Prevention and control technology of pollution in industrial tissue culture had been carried out in Hangzhou and Ningbo， the main tissue culture industry production center in Zhejiang province Two medicines ethylicin and streptomycinterramycin had been screened out for their good control effects on common pollution microflora such as Pseudomonas delafieldii， Pseudomonas syringae， Bacillus cereus， Penicillum digitatum and Aspergillus niger， from 11 germicides and disinfectants And they showed safety to tissue culture seedling and easy to use Ethylicin could be added into culture medium before culture autoclaving for its high temperature resistance， while streptomycinterramycin could only be used in the sterilized culture medium to prevent the growth of contaminants The medicament also had a good disinfection effect for the tissue culture vessel or for the environment. Related Articles | Metrics

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Countermeasures research on introduction of agricultural technology based on the view of late-developing advantage SUN Yongpeng;ZHU Fuyun;WU Weicheng;* 2012, 24(6):  0-1149.  Abstract ( 521 )   PDF (1137KB) ( 956 )   China has obtained some remarkable progress in introduction of foreign advanced agricultural technology，but the low level of efficiency and results are also prominent，it did not form real late-developing advantages after a long time introduction. The paper has studied the main problems in the introduction of foreign advanced agricultural technology including the undefined main bodies and methods， the imperfect selection mechanism， the outdated supporting mechanism and idea etc. We also proposed some countermeasures in the following aspects as to improve the policy environment， strengthen the team construction and accelerate the innovation of mechanism system． Related Articles | Metrics

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Comparison of the modified metagenomic DNA extraction methods of microorganisms from salt lake soils MIAO Liyun;ZHANG Peng;* 2012, 24(6):  0-1090.  Abstract ( 481 )   PDF (1312KB) ( 1030 )   In order to construct the metagenomic library of microorganisms from salt lake soils in Yuncheng， the microbial metagenomic DNA extraction method was explored. Six of plant genomic DNA extraction methods were modified to extract the microbial metagenomic DNA. The yield and quality of the metagenomic DNA was detected by ultraviolet spectrophotometry， gel electrophoresis， restriction endonuclease analysis and PCR amplification， and the results showed that the modified method 3 was suitable for extracting the microbial metagenomic DNA from salt lake soils in Yuncheng. For the microbial metagenomic DNA extracted by the modified method 3， the value of OD260/280 was 1.883， the concentration and yield was 18.1 ng·μL-1 and 72.4 ng·g-1 respectively， and the length was about 23 kb with a clear stripe on the agarose gel. Moreover， the metagenomic DNA was completely digested with EcoRⅠand also could be used for PCR amplification. Related Articles | Metrics

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Effect of different fertilization on yield and quality of bamboo shoots of Dendrocalamopsis oldhami in southern Zhejiang WANG Yueying;XIA Haitao;LI Xiaowen;JIN Chuan;LI Keen;LIN Feng 2012, 24(6):  0-1101.  Abstract ( 457 )   PDF (1165KB) ( 1023 )   Six kinds of fertilizer combination were used to study effect of fertilization on yield and quality of bamboo shoots of Dendrocalamopsis oldhami The results showed that different fertilization had significant influence on yield and quality of bamboo shoots of Dendrocalamopsis oldhami The best effect was found when organicinorganic compound fertilizer was applied in spring and inorganic formula fertilizer was applied in summer Compared with no fertilizer， the yield and quality of bamboo shoots increased by 68.00% and 70.24% respectively， and were 27.3% and 26.5% higher than compound fertilizer， respectively Fertilization twice in spring and summer was more conducive to shoot emergence than fertilization once in spring Fertilization significantly improved nutritional contents， amino acid contents and quality of shoots The results of comprehensive analysis showed that organicinorganic compound fertilizer was applied 4.8 kg each clump in spring， and inorganic formula fertilizer(carbamide 0.4 kg， calcium superphosphate 0.59 kg， K2SO4 0.44 kg， each clump) was applied in summer， which was the best for improving yield and quality of shoots. Related Articles | Metrics

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The anaerobic biodegradation of hexachloroethane under sulfate\|reducing conditions in paddy soils JIN Caixia;ZHAO Baozhen;LI Mingliang;XUE Wanxin;WU Chunyan;*;CHEN Wanqiu 2012, 24(6):  0-1095.  Abstract ( 410 )   PDF (1339KB) ( 1174 )   The degradation of hexachloroethane （HCE） in the paddy soil and the effect of sulfate on sulfate\|reducing bacteria were investigated through simulating different environmental conditions. Results showed that the degradation rate of HCE increased with the increasing initial HCE concentration in the soil， However， the degradation rate of HCE decreased when it reached a certain quality concentration （≥60 mg·kg-1）. The degradation activity of sulfate\|reducing bacteria （SRB） was enhanced and the HCE degradation rate was speeded up with the increasing soil sulfate. But the growth of sulfate\|reducing bacteria and the degradation of HCE were restrained when sulfate was excessive（≥60 mg·kg-1）. The soil pH and Eh values were optimal for the growth of sulfate\|reducing bacteria after 21 days with addition of sulfate and HCE， and then the rate of sulfate degration also reached the maximum. The result showed the degradation of HCE was accelerated through controlling the soil pH and Eh values or adding proper concentration of sulfate to soil in order to reduce the risk of HCE in soil. Related Articles | Metrics

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Study on the temporal and spatial dynamic changes of land use and driving forces analysis of Mongolia Plateau in recent 30 years ZHOU Xiyin;SHI Huading;*;WANG Xiuru;MENG Fanhao 2012, 24(6):  0-1110.  Abstract ( 589 )   PDF (2070KB) ( 1590 )   With the aid of the 3S technology， the land use change data during 1970s and 2005 in Mongolian Plateau were obtained. The spatial and temporal characteristics of the Mongolian Plateau land use change in the past 30 years was revealed by analyzing the changing process. The results showed that the total area of grassland was significantly reduced， a large area of bare land increased， urban and rural construction land expanded significantly， the area of cultivated land， forest land and water increased in Mongolian Plateau in recent 30 years. Grassland degradation and desertification became the significant spatial and temporal characteristics of landuse change in Mongolian Plateau， and the situation of Inner Mongolia was more serious than Mongolia. The forestry ecological engineering in Inner Mongolia played a positive role in protecting the ecological environment. Population， policy， economic and climate change were the main driving forces of the land use dynamic change in Mongolian Plateau． Related Articles | Metrics

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Study on the processing conditions and quality of the whole fruit fermented bayberry wine XING Jianrong;YANG Ying;XIA Qi\le;FANG Xiangjun;CHEN Jianbing;LU Shengmin* 2012, 24(6):  0-1116.  Abstract ( 497 )   PDF (1168KB) ( 1141 )   Bayberry Myrica rubra sieb. et Zucc. cv. Lanxi Muye fruits were used as raw material to study the processing of red bayberry wine fermented by whole fruit. Orthogonal test was used to determine the optimal fermentation conditions and the results indicated that fermentation temperature was 24℃， yeast was added in 015% of the fruit weight， sugar addition amount was 14%， and natural pigment was added with 03%. Quality was also evaluated instrumentally for the whole fruit fermented wine. The physical and chemical items determined of the obtained wine are excellent and follow the quality standard of red bayberry fermented wine. The wine has a good smell of bayberry fruit and typical fermented wine with its color in dark red， appearance in clear and transparent， taste in soft and refreshing and flavor in unique. The product contains 14 kinds of amino acids with the total amounts reaching 830 mg·L-1. Seventeen kinds of main aroma components were detected in the four\|month aging wine， which included 4 kinds of esters， accounting for 29.78% of the ingredients identified， 9 kinds of alcohols， representing 47.51% of the total components and the other 4 flavor substances such as ketone， furan，etc． Related Articles | Metrics

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Method study on the identification of the adulterated camellia oil by gas chromatography-mass spectrometry CHAI Zhenlin;YANG Liu;ZHU Jieli;WU Cuirong;DOU Peiming 2012, 24(6):  0-1121.  Abstract ( 645 )   PDF (1179KB) ( 1081 )   The fatty acid composition of the common edible vegetable oil was analyzed,and the significant changed characteristic components in adulterated camellia oil was also determined. By setting different proportions of adulterating contents and using the method of gas chromatography\|mass spectrometry (GC\|MS),the regression equation was established between the characteristic components contents after adulterated of palm oil, rapeseed oil, cotton seed oil, peanut oil and soybean oil in camellia oil and the adulterated amount. The correlation coefficient was greater than 099,and the overall accuracy was 101%. This method can be used for the qualitative identification and quantitative detection of adulterated camellia oil． Related Articles | Metrics

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Quantitative analysis of structural changes on peasants income and its effect in the underdeveloped areas HUNAG Yanxian 2012, 24(6):  0-1153.  Abstract ( 407 )   PDF (1423KB) ( 1064 )   Peasants income increase is the core of the “three agricultural problems” The structure of peasants income changes with the increase of peasants income， and the structural changes of peasants income can reflect the quantitative change of peasants income The paper analyzed the process of the structural changes in the peasants income from different sources of income， and revealed the relationship between the structural changes of peasants income and peasants income increase by structure change analysis and contribution analysis. Related Articles | Metrics

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Review of technical barriers to trade (TBT) on bee products in China SUN Caixia;ZHANG Zhiheng*;TANG Tao;YUAN Yuwei;YANG Guiling;ZHENG Weiran 2012, 24(6):  0-1133.  Abstract ( 456 )   PDF (1149KB) ( 1102 )   In comparison with developed countries， Chinese quality regulations of bee products were different in pesticide and veterinary management， heavy metals， microorganism， grades and specifications The differences were the reasons of some technical barriers to trade in international bee product commerce On the analysis of WTO/TBTSPS notification， the regulations for quality and safety， especially on antibiotics， are the key detention reasons of bee products exported to developed countries Good measures should be taken in production process， including right pesticide application， guarantee measures of the quality and safety， and thus to promote the bee industry develop well. Related Articles | Metrics

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Optimization of cellulase production by Aspergillus niger separated from tobacco and its separation and purification FAN Jianqiang;ZHOU Bin;SONG Jizhen;WANG Jinhua;*;CHEN Yiqiang;WANG Yongze 2012, 24(6):  0-1128.  Abstract ( 468 )   PDF (1434KB) ( 1237 )   In order to improve tobacco taste， decrease the smoking pungent taste， increase the fragrance and improve the smoking quality， a mold strain Aspergillus niger possessing relatively higher cellulase enzymatic activity was isolated from tobacco leaves The PlackettBurman design was used to choose the most important factors， then， these factors were optimized by RSM method， and the purification of enzyme was investigated The results indicated that the optimum carbon source and nitrogen source were 1.4% corn cob and 0.4% NH4NO3， the optimum original pH was 5.6 and the optimum cultural temperature was 30.4℃ Under these conditions， the specific enzyme activity was 40.02 U·mL-1 The crude cellulase was isolated and purified by means of ammonium sulfate precipitation， followed by chromatography on Sephadex G100 The specific activity of the cellulase increased to 3.5 fold of the source enzyme with an activity recovery of 32.6%. Related Articles | Metrics

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Analysis of food production pattern in Jiangxi Province LIU Pengfa;CHEN Saidi;MA Yongjun;*;ZOU Jiajia 2012, 24(6):  0-1139.  Abstract ( 482 )   PDF (1487KB) ( 1142 )   According to the statistical data of grain production， with the index model such as time series， coefficient of variation， geography relation ratio and location quotient model， this article discussed the grain production and development and the change characteristics of the regional pattern in Jiangxi province The regional advantages of eight kinds of grain crops in Jiangxi province was analyzed by scale advantage index， efficient advantage index and comprehensive advantage index It showed that grain production in Jiangxi Province experienced a process pattern of risingstablerising during 1978 to 2010 The discrete degree and regional difference increased， but the geography relation ratio of grainyield & population number declined， and Jian， Yichun，Fuzhou and etc became the center of grain production The significant difference exists in different production areas in Jiangxi At last the suggestion for adjustment of grain crops pattern was proposed. Related Articles | Metrics

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Construction of modern agriculture geographic information system in Zhejiang and its effects DONG Yueyong;GUAN Xiaofeng;TAO Zhongliang;LYU Xiaonan;REN Zhouqiao 2012, 24(6):  0-1145.  Abstract ( 510 )   PDF (1335KB) ( 1420 )   In order to fully play the role of agricultural information in modern agricultural production and operation as science and technology demonstration and support， effectively strengthen the scientific management of food production function area， modern agricultural garden and standard farmland (hereafter referred to as “two areas and one farmland”)， the construction of modern agriculture geographic information system in Zhejiang was carried out This paper mainly introduced the content， technical protocol， progress and effect of the modern agriculture geographic information system At present， the first period of software development and the experimental system construction in 9 districts were almost completed and achieved some results in guaranteeing the scientific decision of government department， realizing the information management of agricultural production factors， promoting service ability of agricultural department， regulating the administrative management activities， reducing the administrative operation and maintenance costs and so on， and soma functions including the production factor management， agricultural project supervision， realtime monitoring scheduling and network science decision were preliminary accomplished. Related Articles | Metrics

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Empirical research on the relationship between financial interrelations ratio and optimization of marine industry structure in China YAO Jianfeng;YANG Deli* 2012, 24(6):  0-1158.  Abstract ( 464 )   PDF (1158KB) ( 1100 )   With the sustainable and rapid development of marine economy， the marine industry structure has been rationalized and highly optimized in recent decades Whether the marine industry structure is rationalized or not is of vital importance to the marine economy of a country Although the marine economy of China has made great strides in development， there still exists many factors which restrict further optimization and upgrading of marine industry structure Finance is one of the most important influencing factors Financial interrelations ratio and the value of marine industry structure were taken as research objects， the conclusion that financial support does Granger cause optimization of marine industrystructure was made by Granger causality test It was suggested that the government should construct a favorable financial environment， fully played the role of financial support for the development of marine industry structure， and actively led private capital to the second and third marine industry， in order to promote the optimization and upgrading of marine industry structure. Related Articles | Metrics

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Estimation on land consolidation potential of rural residential area in Cheng County WANG Qi;ZHANG Renzhi*;CHEN Ying 2012, 24(6):  0-1163.  Abstract ( 459 )   PDF (1146KB) ( 1154 )   The purpose of this paper is to estimate the potential of rural residential land consolidation of Cheng County which can provide references for the work and the special plan of rural residential and consolidation Standard method of construction land per capita， multifactor comprehensive assessment and AHP were employed The results showed that the theoretical potential and the realistic potential value of rural residential land consolidation in this region were 2261.66 hm2 and 603.69 hm2， and the comprehensive correction coefficients were ranged from 0.1009 to 0.4175 Due to various constraints， the corrected value accounted for 2669% of the theoretical value of the potential of rural residential land consolidation It was necessary to act according to these results to improve land consolidation efficiency. Related Articles | Metrics

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Pollution of livestock and poultry feces and countermeasures GUO Dongsheng;PENG Xiaolan;GONG Qunhui;XIA Weifu 2012, 24(6):  0-1170.  Abstract ( 494 )   PDF (1171KB) ( 2162 )   Along with the development of livestock industry to the intensive, large-scale and specialized direction, heavy metal elements, pathogenic microbes, nitrogen, phosphorus, ammonia, hydrogen sulfide, methane and toxic and harmful gas in livestock and poultry manure caused serious environmental pollution and harm to water, air and soil environment, which was an urgent important issue. This paper analyzed the pollution and harm of livestock and poultry manure in the view of current situation of poultry pollution, discussed the reduction, harmlessness and recycling utilization measures and methods of the livestock and poultry manure． Related Articles | Metrics