Abstract: Cymbidium mosaic virus (CymMV) is an important virus infecting Orchidaceous plants severely In this study， according to the genomic sequence of CymMV in GenBank (Accession No AB197937)， the specific primers were designed for amplifying its TGB1 and TGB2 gene， respectively By reverse transcriptionpolymerase chain reaction (RTPCR)， the TGB1 and TGB2 genes were amplified from CymMVinfected Phalaenopsis leaves Both fragments were then inserted into pGEX4T3 vector for prokaryotic expression The constructed expression vectors were transformed into E coli BL21 that was induced by IPTG for expressing the proteins SDSPAGE and Western blot analysis confirmed that the expressed proteins were GSTfused CymMV TGB1 and TGB2 proteins Expression of TGB1 and TGB2 will help to prepare the antibodies for their further functional analysis.

Key words: Phalaenopsis, CymMV, prokaryotic expression, Western blot