Abstract: The PCRSSCP was used to study the associations between immune traits(sheep red blood cell, avian influenza and newcastle disease)and polymorphic loci of the Mx gene in Shandong indigenous chicken populations(Luqin chicken, Shi qiza chicken, Langya chicken, Laiwu black chicken and Jining Bairi chicken). The fragment of Mx gene about 150 bp including intron 13 and exon 14 was amplified. The results showed that two single nucleotide polymorphisms were detected in the five indigenous chicken populations by PCRSSCP: 1 892 points of coding sequence of Mx gene G→A (Ser→Asn); 1 911 points G→A, which couldnt lead to amino acid mutation. Through the analysis on the association between the polymorphic loci of the exon 14 and the antibody response, the SNPs on the 1 892 points of coding sequence of Mx gene in Luqin chicken, Langya chicken and Laiwu black chicken was correlated to antibody response of H9 significantly(P＜0.05).

Key words: chicken, Mx gene, PCRSSCP, immune traitsMx