Abstract: Prevention and control technology of pollution in industrial tissue culture had been carried out in Hangzhou and Ningbo， the main tissue culture industry production center in Zhejiang province Two medicines ethylicin and streptomycinterramycin had been screened out for their good control effects on common pollution microflora such as Pseudomonas delafieldii， Pseudomonas syringae， Bacillus cereus， Penicillum digitatum and Aspergillus niger， from 11 germicides and disinfectants And they showed safety to tissue culture seedling and easy to use Ethylicin could be added into culture medium before culture autoclaving for its high temperature resistance， while streptomycinterramycin could only be used in the sterilized culture medium to prevent the growth of contaminants The medicament also had a good disinfection effect for the tissue culture vessel or for the environment.

Key words: plant tissue culture, pollution control technology, ethylicin, streptomycinterramycin