›› 2012, Vol. 24 ›› Issue (6): 0-1063.

• 论文 •    

Biocontrol potential of Caenorhabditis sp CNM strain discovered from the compost of Agaricus bisporus

LI Xingyue;LIU Qizhi*;YANG Daowei   

  1. Laboratory of Entomology and Nematology,College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-11-25 Published:2012-11-25

Abstract: One species of insect nematode (Caenorhabditis sp CNM), which can cause Galleria mellonella death within 4 days, was discovered in the compost of Agaricus bisporus through the Galleria bait method In order to discover the biologicalcontrol potential of CNM in the field application, we investigated its biology characteristics, especially the infectivity and antistress resistance The result of its infectivity to G mellonella showed that the larger of the dosage, the better of its infectivity LD50 of CNM to G mellonella was 46.6 IJs per larva, and the LC50 is 53.8 IJs·cm-2 at 48 h After 45 min, 20 h and 55 h, all of the nematodes died with water bathing of 42℃, 40℃ and 38℃ Therere still 46.0% of the nematodes alive after 12 h under 25℃, 50.5 % RH, and the infectivity decrease was 43.3% Besides, there were re 57.1% of nematode that can move for as far as 3.6 cm under 30℃ So Caenorhabditis sp CNM strain has the biocontrol potential with good infectivity, heat resistance and desiccation.

Key words: Agaricus bisporus, Caenorhabditis, biocontrol, infectivity, antistress resistance