Identification of Lycium barbarum root rot disease pathogens and biocontrol funguses against root rot disease

doi:10.3969/j.issn.1004-1524.20231082

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (10): 2283-2297.DOI: 10.3969/j.issn.1004-1524.20231082

• Plant Protection • Previous Articles Next Articles

Identification of Lycium barbarum root rot disease pathogens and biocontrol funguses against root rot disease

JI Songyan¹(), SHAO Changqi¹, QI Wenkang¹, HE Yuhui¹, ZHANG Xin¹^,², WANG Cuiping¹^,³^,⁴^,^*()

1. School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China
2. Innovation Team for Genetic Improvement of Economic Forests, North Minzu University, Yinchuan 750021, China
3. State Key Laboratory of Efficient Production of Forest Resources, Yinchuan 750004, China
4. Ningxia Grape and Wine Technology Innovation Center, Yinchuan 750021, China

Received:2023-09-08 Online:2024-10-25 Published:2024-10-30

Abstract

Abstract:

Root rot disease of Lycium barbarum is a common soil-borne disease that occurs in L. barbarum cultivation areas. It is caused by a complex infection of multiple pathogenic fungi and is currently primarily controlled through chemical means. The aim of this study is to explore the feasibility of rapid screening of highly effective, broad-spectrum and practical biocontrol fungi with disease prevention and growth promotion effects, and identify the main pathogenic bacteria of L. barbarum root rot in Ningxia region and screen excellent antagonistic strains. Using the diseased root of Ningqi No.1 L. barbarum and the rhizosphere soil as materials, pathogenic funguses and rhizosphere microorganisms were isolated and purified using tissue separation method; the inhibitory effect of rhizosphere microorganisms on isolated pathogenic funguses was determined using the confrontation method; the antagonistic fungi was identified based on colony culture, morphological characteristics, and rDNA-ITS sequence. The results showed that the main strains isolated from the diseased root tissues of L. barbarum were Fusarium oxysporum, Fusarium solani, Fusarium dimrum, Fusarium equiseti, Fusarium petroliphilum, and Mortierella alpina, with frequencies of 29.5%, 16.8%, 13.7%, 8.9%, 8.9%, and 7.4%, respectively. Aspergillus terreus and Trichoderma longibrachiatum have obvious inhibition effect on the main pathogenic funguses, with the inhibition rate of more than 37%, which can effectively adjust the microbial flora of the root zone of L. barbarum and promote the restoration and balance of soil micro-ecology.

Key words: Lycium barbarum, root rot disease, pathogen, biocontrol funguses, Fusarium oxysporum, Aspergillus terreus, Trichoderma longibrachiatum

CLC Number:

S436.65

JI Songyan, SHAO Changqi, QI Wenkang, HE Yuhui, ZHANG Xin, WANG Cuiping. Identification of Lycium barbarum root rot disease pathogens and biocontrol funguses against root rot disease[J]. Acta Agriculturae Zhejiangensis, 2024, 36(10): 2283-2297.

Figures/Tables 11

References 37

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编号 No.	菌株 Strain	分离数 Number	分离率 Rate/%
Ⅰ	N. rubicola	9	4.7
Ⅱ	A. niger	7	3.7
Ⅲ	F. dimerum	26	13.7
Ⅳ	F. falciforme	6	3.2
Ⅴ	F. solani	32	16.8
Ⅵ	M. alpina	14	7.4
Ⅶ	F. oxysporum	56	29.5
Ⅷ	F. equiseti	17	8.9
Ⅸ	F. petroliphilum	17	8.9
Ⅹ	A. carneus	5	2.6
ⅩⅠ	S. lignicola	1	0.5

编号 No.	菌株 Strain	分离数 Number	分离率 Rate/%
Ⅰ	N. rubicola	9	4.7
Ⅱ	A. niger	7	3.7
Ⅲ	F. dimerum	26	13.7
Ⅳ	F. falciforme	6	3.2
Ⅴ	F. solani	32	16.8
Ⅵ	M. alpina	14	7.4
Ⅶ	F. oxysporum	56	29.5
Ⅷ	F. equiseti	17	8.9
Ⅸ	F. petroliphilum	17	8.9
Ⅹ	A. carneus	5	2.6
ⅩⅠ	S. lignicola	1	0.5

致病菌 Pathogenic fungi	发病情况 Incidence	致病性 Pathogenicity
B9(N. rubicola)	3条根被侵染变为棕色,根上产生少量黄色液滴 Three roots were infected and turned brown, with a small amount of yellow droplets produced on the roots	++
N7(A. niger)	12条根全被侵染变为黑色,且大量腐烂成产生黄色液滴 All 12 roots were infected and turned black, and a large number of them decayed into yellow droplets	++++
A1(F. dimerum)	无明显效果No significant effect
A4(F. falciforme)	无明显效果No significant effect
A7(F. solani)	12条根全被侵染变为棕色,且产生少量黄色液滴 All 12 roots were infected and turned brown, with a small amount of yellow droplets produced	+++
A8(M. alpina)	2条根被侵染变为棕色,根上产生少量黄色液滴 Two roots were infected and turned brown, with a small amount of yellow droplets produced on the roots	++
B2c(F. oxysporum)	12条根全被侵染变为棕色,且大量腐烂成产生黄色液滴 All 12 roots were infected and turned brown, with a large amount of decay resulting in the production of yellow droplets	++++
B2d(F. equiseti)	4条根被侵染变为棕色,根周围产生大量黄色液滴,根上长出黑色斑点 Four roots were infected and turned brown, with a large number of yellow droplets produced around the roots and black spots growing on the roots	+++
B2(F. solani)	12条根全被侵染变褐色,且根周围产生大量黄色液滴 All 12 roots were infected and turned brown, and a large number of yellow droplets were produced around the roots	++++
B4(A. carneus)	12条根全被侵染变褐色,少量变为黑色,且部分腐烂成黄色液滴 All 12 roots were infected and turned brown, with a small amount turning black, and some rotting into yellow droplets	++++
对照Control	12 条根全部保持正常浅黄色All 12 roots remain normal and light yellow in color

致病菌 Pathogenic fungi	发病情况 Incidence	致病性 Pathogenicity
B9(N. rubicola)	3条根被侵染变为棕色,根上产生少量黄色液滴 Three roots were infected and turned brown, with a small amount of yellow droplets produced on the roots	++
N7(A. niger)	12条根全被侵染变为黑色,且大量腐烂成产生黄色液滴 All 12 roots were infected and turned black, and a large number of them decayed into yellow droplets	++++
A1(F. dimerum)	无明显效果No significant effect
A4(F. falciforme)	无明显效果No significant effect
A7(F. solani)	12条根全被侵染变为棕色,且产生少量黄色液滴 All 12 roots were infected and turned brown, with a small amount of yellow droplets produced	+++
A8(M. alpina)	2条根被侵染变为棕色,根上产生少量黄色液滴 Two roots were infected and turned brown, with a small amount of yellow droplets produced on the roots	++
B2c(F. oxysporum)	12条根全被侵染变为棕色,且大量腐烂成产生黄色液滴 All 12 roots were infected and turned brown, with a large amount of decay resulting in the production of yellow droplets	++++
B2d(F. equiseti)	4条根被侵染变为棕色,根周围产生大量黄色液滴,根上长出黑色斑点 Four roots were infected and turned brown, with a large number of yellow droplets produced around the roots and black spots growing on the roots	+++
B2(F. solani)	12条根全被侵染变褐色,且根周围产生大量黄色液滴 All 12 roots were infected and turned brown, and a large number of yellow droplets were produced around the roots	++++
B4(A. carneus)	12条根全被侵染变褐色,少量变为黑色,且部分腐烂成黄色液滴 All 12 roots were infected and turned brown, with a small amount turning black, and some rotting into yellow droplets	++++
对照Control	12 条根全部保持正常浅黄色All 12 roots remain normal and light yellow in color

菌株 Strain	生长抑制率Percentage of growth inhibition
菌株 Strain	B9	A1	A7	B2c	B4
PDA-2	50.53±1.53	47.53±2.46	37.87±0.92	57.23±1.79	51.67±1.26
B1	45.58±1.53	37.93±1.33	36.12±1.20	42.29±1.79	51.11±1.40

Identification of Lycium barbarum root rot disease pathogens and biocontrol funguses against root rot disease

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