Pathogen identification of root rot of Curcuma wenyujin

doi:10.3969/j.issn.1004-1524.20230797

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (5): 1086-1093.DOI: 10.3969/j.issn.1004-1524.20230797

• Plant Protection • Previous Articles Next Articles

Pathogen identification of root rot of Curcuma wenyujin

LI Yani^a(), CHEN Weiliang^a^,^*(), MAO Bizeng^b^,^*()

a. Institute of Biotechnology, Key Lab of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province; b. The Rural Development Academy, Zhejiang University, Hangzhou 310058, China

Received:2023-06-27 Online:2024-05-25 Published:2024-05-29

Abstract

Abstract:

At main production areas of Curcuma wenyujin in Taoshan Town, Rui’an City, Zhejiang Province, large-scale lodging of C. wenyujin and severe rot of their underground tubers occurred in some fields. To identify the pathogen that causes the disease, the bacteria strain JF1 was isolated from the rotten tuber of C. wenyujin by using LB and TTC (2,3,5-triphenyltetrazolium chloride) medium. The pathogenicity of isolated strain JF1 was confirmed by using three inoculation methods, including root irrigation, bud infection and rhizome infection. The taxonomic status of pathogenic bacteria was identified according to the morphological characteristics, physiological and biochemical tests, phylogenetic analysis of multiple gene sequences (16S rDNA, rpoB, gyrB). The results showed that the pathogenic bacteria causing root rot of C. wenyujin was Enterobacter mori.

Key words: Curcuma wenyujin, root rot, pathogen identification, Enterobacter mori

CLC Number:

S435.672

LI Yani, CHEN Weiliang, MAO Bizeng. Pathogen identification of root rot of Curcuma wenyujin[J]. Acta Agriculturae Zhejiangensis, 2024, 36(5): 1086-1093.

Figures/Tables 7

References 18

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基因 Gene	引物名称 Primer	引物序列 Primer sequence(5'→3')	片段大小 Fragment size/bp
16S rDNA	27F	AGAGTTTGATCCTGGCTCAG	1 500
	1492R	TACGGCTACCTTGTTACGACTT
rpoB	CM7	AACCAGTTCCGCGTTGGCCTGG	1 009
	CM31	CCTGAACAACACGCTCGGA
gyrB	UP-1	GAAGTCATCATGACCGTTCTGCAYGCNGGNGGNAARTTYGA	1 200
	UP-2r	AGCAGGGTACGGATGTGCGAGCCRTCNACRTCNGCRTCNGTCAT

基因 Gene	引物名称 Primer	引物序列 Primer sequence(5'→3')	片段大小 Fragment size/bp
16S rDNA	27F	AGAGTTTGATCCTGGCTCAG	1 500
	1492R	TACGGCTACCTTGTTACGACTT
rpoB	CM7	AACCAGTTCCGCGTTGGCCTGG	1 009
	CM31	CCTGAACAACACGCTCGGA
gyrB	UP-1	GAAGTCATCATGACCGTTCTGCAYGCNGGNGGNAARTTYGA	1 200
	UP-2r	AGCAGGGTACGGATGTGCGAGCCRTCNACRTCNGCRTCNGTCAT

菌株 Strain	GenBank登录号GenBank accession No.
菌株 Strain	16S rDNA	rpoB	gyrB
Enterobacter mori LMG 25706 Enterobacter quasiroggenkampii WCHECL1060	MG846019 KY979139	EU579858 KY979141	JX424992 KY979140
Enterobacter roggenkampii DSM 16690	OP818082	CP017184	CP017184
Enterobacter chengduensis WCHECl-C4	NR179167	KY979144	KY979143
Enterobacter wuhouensis WCHEs120002	MK567957	MK613102	MK613100
Enterobacter chuandaensis 090028	MK049966	MK056243	MK056241
Enterobacter sichuanensis WCHECL1597	MG832788	MG832790	MG832789
Enterobacter bugandensis EB-247	MK603177	LT992502	LT992502
Enterobacter quasihormaechei WCHEs120003	NR180451	MK613106	MK613104
Enterobacter kobei DSM 13645	LT547823	CP017181	CP017181
Enterobacter hormaechei ATCC 49162	OP818074	AJ543724	AY370840
Enterobacter asburiae ATCC 35953	OP818065	AJ543727	CP011863
Enterobacter ludwigii EN-119	MK603179	CP017279	CP017279
Enterobacter cancerogenus LMG 2693	NR116756	AJ566943	FJ617354
Enterobacter oligotrophicus CCA6	OP818079	AP019007	AP019007
Enterobacter cloacae ATCC 13047	NR118568	AJ543726	AB084011
Enterobacter huaxiensis 090008	NR180236	MK056242	MK056248
Enterobacter xiangfangensis 10-17	NR126208	HF679054	CP017183

菌株 Strain	GenBank登录号GenBank accession No.
菌株 Strain	16S rDNA	rpoB	gyrB
Enterobacter mori LMG 25706 Enterobacter quasiroggenkampii WCHECL1060	MG846019 KY979139	EU579858 KY979141	JX424992 KY979140
Enterobacter roggenkampii DSM 16690	OP818082	CP017184	CP017184
Enterobacter chengduensis WCHECl-C4	NR179167	KY979144	KY979143
Enterobacter wuhouensis WCHEs120002	MK567957	MK613102	MK613100
Enterobacter chuandaensis 090028	MK049966	MK056243	MK056241
Enterobacter sichuanensis WCHECL1597	MG832788	MG832790	MG832789
Enterobacter bugandensis EB-247	MK603177	LT992502	LT992502
Enterobacter quasihormaechei WCHEs120003	NR180451	MK613106	MK613104
Enterobacter kobei DSM 13645	LT547823	CP017181	CP017181
Enterobacter hormaechei ATCC 49162	OP818074	AJ543724	AY370840
Enterobacter asburiae ATCC 35953	OP818065	AJ543727	CP011863
Enterobacter ludwigii EN-119	MK603179	CP017279	CP017279
Enterobacter cancerogenus LMG 2693	NR116756	AJ566943	FJ617354
Enterobacter oligotrophicus CCA6	OP818079	AP019007	AP019007
Enterobacter cloacae ATCC 13047	NR118568	AJ543726	AB084011
Enterobacter huaxiensis 090008	NR180236	MK056242	MK056248
Enterobacter xiangfangensis 10-17	NR126208	HF679054	CP017183

编号 Number	特性 Property	结果 Result	分离菌株JF1 Isolated strain JF1	模式菌株E. mori Type strain E. mori	阴性对照 Negative control
1	流动性Motility	穿刺线周围呈雾状Foggy puncture line	+	+	-
2	鸟氨酸脱羧酶Ornithine decarboxylase	紫色Purple	+	+	-
3	赖氨酸脱羧酶Lysine decarboxylase	黄色Yellow	-	-	-
4	甲基红试验Methyl red test	不变色Unchanged	-	-	-
5	V-P试验Voges-Proskauer test	红色Red	+	+	-
6	西蒙氏枸橼酸盐Simmons citrate	蓝色Blue	+	+	-
7	硫化氢生成H₂S production	不变色Unchanged	-	-	-
8	尿素酶Urease	黄色Yellow	-	-	-
9	色氨酸Tryptophan	不变色Unchanged	-	-	-
10	苯丙氨酸Phenylalanine	不变色Unchanged	-	-	-
11	甘露醇Mannitol	黄色Yellow	+	+	-
12	肌醇Inositol	绿色Green	-	-	-
13	山梨醇Sorbitol	黄色Yellow	+	+	-
14	蜜二糖Melibiose	黄色Yellow	+	+	-
15	核糖醇Ribitol	绿色Green	-	-	-
16	棉子糖Raffinose	黄色Yellow	+	+	-

Pathogen identification of root rot of Curcuma wenyujin

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