Identification and control of anthracnose in vegetable soybean

doi:10.3969/j.issn.1004-1524.2022.12.11

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (12): 2682-2688.DOI: 10.3969/j.issn.1004-1524.2022.12.11

• Plant Protection • Previous Articles Next Articles

Identification and control of anthracnose in vegetable soybean

LIU Na(), FAN Qiaochu, ZHOU Jia, SONG Yajing, ZHANG Guwen, FENG Zhijuan, BO Yuanpeng, WANG Bin, GONG Yaming()

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2021-09-18 Online:2022-12-25 Published:2022-12-26
Contact: GONG Yaming

Abstract

Abstract:

Anthracnose disease is a common fungal disease of vegetable soybean, which forms black spots on the pod surface and affects the appearance and quality of fresh pod seriously. It has become one of economically important fungal diseases on vegetable soybean yield in China. To identify the pathogens causing vegetable soybean anthracnose in Zhejiang Province, we collected anthracnose disease samples and isolated the pathogens according to Koch's postulate. The pathogens were further identified based on morphological characteristics and rDNA-ITS sequence analysis in this study. Our results showed that two Colletotrichum species, Colletotrichum truncatum and Colletotrichum gloeosporioides, were the major pathogens causing vegetable soybean anthracnose in this region. To further guide the vegetable soybean anthracnose control using fungicides, we measured the sensitivity of representative strain C. truncatum Cts18 and C. Gloeosporioides Cts22 to commonly used fungicide carbendazim and tebuconazole. Results indicated that the concentration for 50% of maximal effect (EC₅₀) of carbendazim against C. truncatum Cts18 and C. gloeosporioides Cts22 were 2.13 μg·mL^-1 and 96.12 μg·mL^-1, respectively. The EC₅₀ of tebuconazole against Cts18 and Cts22 were 0.27 μg·mL^-1 and 0.63 μg·mL^-1, respectively. These results suggested that Cts18 was more sensitive than Cts22 towards the tested fungicides, and tebuconazole is a potential fungicide for managing vegetable soybean anthracnose in fields in this region.

Key words: vegetable soybean, anthracnose, pathogen identification, Colletotrichum truncatum, Colletotrichum gloeosporioides, fungicide sensitivity

CLC Number:

S643.7

LIU Na, FAN Qiaochu, ZHOU Jia, SONG Yajing, ZHANG Guwen, FENG Zhijuan, BO Yuanpeng, WANG Bin, GONG Yaming. Identification and control of anthracnose in vegetable soybean[J]. Acta Agriculturae Zhejiangensis, 2022, 34(12): 2682-2688.

Figures/Tables 7

References 20

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种 Species	菌株 Isolates	ITS序列长度 Length of ITS/bp	GenBank序列号 GenBank number
Colletotrichum truncatum	CF-2-1	558	MK611675.1
Colletotrichum capsici	GK17A	584	MK713419.1
Colletotrichum jasminigenum	Co-15-Coy	560	JQ658867.1
Colletotrichum dematium	IMI-080025	580	AB046608.1
Colletotrichum siamense	C-106	606	MG913611.1
Colletotrichum gloeosporioides	WZ-244	583	MN856366.1
Colletotrichum lindemuthianum	GY3	575	MF511774.1
Colletotrichum sichuanense	CMM2902	549	MF279904.1
Colletotrichum cliviae	AGMy0252	594	KX578803.1
Colletotrichum magnum	Cmj2	555	KT949410.1
Pseudevernia furfuracea	M1_H21	503	MN654498.1

种 Species	菌株 Isolates	ITS序列长度 Length of ITS/bp	GenBank序列号 GenBank number
Colletotrichum truncatum	CF-2-1	558	MK611675.1
Colletotrichum capsici	GK17A	584	MK713419.1
Colletotrichum jasminigenum	Co-15-Coy	560	JQ658867.1
Colletotrichum dematium	IMI-080025	580	AB046608.1
Colletotrichum siamense	C-106	606	MG913611.1
Colletotrichum gloeosporioides	WZ-244	583	MN856366.1
Colletotrichum lindemuthianum	GY3	575	MF511774.1
Colletotrichum sichuanense	CMM2902	549	MF279904.1
Colletotrichum cliviae	AGMy0252	594	KX578803.1
Colletotrichum magnum	Cmj2	555	KT949410.1
Pseudevernia furfuracea	M1_H21	503	MN654498.1

化学药剂 Fungicides categories	菌株 Strain	毒力回归方程 Regression equation	EC₅₀/ (μg· mL^-1)	r
多菌灵 Carbendazim	Cts18	Y=0.25+0.69x²	2.13	0.90
	Cts22	Y=2.85+1.39x²	96.12	0.91
戊唑醇 Tebutazolol	Cts18	Y=0.46+0.81x²	0.27	0.98
	Cts22	Y=0.28+1.40x²	0.63	0.97

化学药剂 Fungicides categories	菌株 Strain	毒力回归方程 Regression equation	EC₅₀/ (μg· mL^-1)	r
多菌灵 Carbendazim	Cts18	Y=0.25+0.69x²	2.13	0.90
	Cts22	Y=2.85+1.39x²	96.12	0.91
戊唑醇 Tebutazolol	Cts18	Y=0.46+0.81x²	0.27	0.98
	Cts22	Y=0.28+1.40x²	0.63	0.97

Identification and control of anthracnose in vegetable soybean

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