Identification of tobacco anthracnose and isolation of antagonistic Streptomyces

doi:10.3969/j.issn.1004-1524.2019.08.15

›› 2019, Vol. 31 ›› Issue (8): 1337-1344.DOI: 10.3969/j.issn.1004-1524.2019.08.15

• Plant Protection • Previous Articles Next Articles

Identification of tobacco anthracnose and isolation of antagonistic Streptomyces

LI Jiye¹, WANG Jingjing¹, YANG Lu¹, PENG Lijuan^{2,3, *}

1. College of Agriculture, Guizhou University, Guiyang 550025, China;
2. Guizhou Key Laboratory for Tobacco Quality, Guiyang 550025, China;
3. College of Tobacco Science, Guizhou University, Guiyang 550025, China

Received:2019-01-22 Online:2019-08-25 Published:2019-08-30

Abstract

Abstract: A strain of tobacco anthracnose pathogen was isolated from Guizhou Province. In order to clarify the taxonomic status in the genus of Colletotrichum and investigate the suitable Streptomyces biocontrol strain, morphology and polygenic phylogenetic analysis were performed. The biological characteristics were studied and antagonistic Streptomyces were isolated. Results showed that the pathogen was identified as Colletotrichum orbiculare. The best carbon source and nitrogen source for growth was starch and peptone, but it couldn't use oxamic acid. The optimum temperature for growth was 25 ℃. Illumination and pH had little effect on colony diameter. Two strains of Streptomyces F18 and F35 with good antagonistic effects were isolated and preliminarily identified as Streptomyces albolongus and Streptomyces plumbiresistens.

Key words: tobacco, anthracnose, antagonistic, Streptomyces

CLC Number:

S435.72

LI Jiye, WANG Jingjing, YANG Lu, PENG Lijuan. Identification of tobacco anthracnose and isolation of antagonistic Streptomyces[J]. , 2019, 31(8): 1337-1344.

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Identification of tobacco anthracnose and isolation of antagonistic Streptomyces

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