Screening, identification of Phytophthora infestans and its antagonistic bacterial strain

doi:10.3969/j.issn.1004-1524.2020.05.12

›› 2020, Vol. 32 ›› Issue (5): 840-848.DOI: 10.3969/j.issn.1004-1524.2020.05.12

• Plant Protection • Previous Articles Next Articles

Screening, identification of Phytophthora infestans and its antagonistic bacterial strain

LIU Ziying¹, YUAN Bin², XIAO Huamei², WU Yongfei¹, LIU Xiaolin^2,*, HU Xiangfei¹

1. School of Chemical and Biological Engineering, Yichun University, Yichun 336000, China;
2. College of Life Science and Resources Environment, Yichun University, Yichun 336000, China

Received:2020-01-16 Online:2020-05-25 Published:2020-05-29

Abstract

Abstract: Phytophthora infestans was identified from potato via tissue isolation method and the antibacterial effects of three antagonistic bacterium to it were compared in order to get the potential one. They were isolated by coating method and identified by morphology and molecular biology method. The inhibitory effects of Phytophthora infestans were studied including the release of zoospore, germination of resting spore and germination of sporangium. The preventive effect of the bacterial solution was also analyzed. The results showed that a PLB-2 Phytophthora infestans and a WZ-502 Brachybacterium sp. of potato were separated and identified. The strain WZ-502 had significant inhibitory effect on the release of zoospores, direct germination of sporangium and germination of resting spores. The strain WZ-502 (concentration was 3.65×10⁷ CFU·mL^-1) at dilutions grade of 10^-2-10^-3 was effective, and the rates of sporangium germination and the release of Phytophthora infestans were both below 40% when the dilution was 10^-3. The strain WZ-502 had a good antibacterial effect on Phytophthora infestans.

Key words: potato, Phytophthora infestans, antagonistic bacteria, zoospores release, sporangium germination

CLC Number:

S435.3

LIU Ziying, YUAN Bin, XIAO Huamei, WU Yongfei, LIU Xiaolin, HU Xiangfei. Screening, identification of Phytophthora infestans and its antagonistic bacterial strain[J]. , 2020, 32(5): 840-848.

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Screening, identification of Phytophthora infestans and its antagonistic bacterial strain

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