Pathogenic diagnosis of synergistic infection of potato mosaic disease

doi:10.3969/j.issn.1004-1524.2018.01.13

›› 2018, Vol. 30 ›› Issue (1): 99-105.DOI: 10.3969/j.issn.1004-1524.2018.01.13

• Plant Protection • Previous Articles Next Articles

Pathogenic diagnosis of synergistic infection of potato mosaic disease

SONG Xijiao^{1, 2}, XIE Li^{1, 2}, XUAN Yuji³, WANG Fang⁴, YAN Chengqi¹, CHEN Jianping^{1, *}

1. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. Public Lab, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. Ningbo Yinzhou Seed Extention Station, Ningbo 315100, China;
4. Institute of Biotechnology, Ningbo Academy of Agricultural Sciences, Ningbo 315101, China

Received:2017-06-19 Online:2018-01-20 Published:2018-02-09

Abstract

Abstract: In 2015, an infected potato plant was found in Zhejiang Ningbo, which had the typical symptom of dwarf and mosaic leaf was thought to be a potato viral disease. Two kinds of linear virus particles with the typical length of 600-900 and 400 nm were observed under transmission electron microscopy by the negative staining of infected potato leaves. Ultra-thin section revealed that viral products were accumulated in the mesophyll cells. Except for observing the virus like particles in cytoplasm, two other kinds of inclusion bodies could also be observed in one cell including the cylindrical inclusion (CI) body and the laminated inclusion component (LIC), which was induced by two kinds of different viruses, CI induced by Potyvirus and LIC induced by Potexvirus, implying a doubly infected in this plant. To verify the pathogen, a molecular assay was performed by RT-PCR and sequencing, by using of the universal primers of Potyvirus and the specific primers of Potexvirus. At last, with the positive results of pathogenic diagnosis, we confirmed that this sick potato was induced by a synergistic infection with two viruses of potato virus X(PVX) and potato virus Y (PVY).

Key words: potato mosaic disease, potato virus X, potato virus Y, synergitic infection, pathogenic diagnosis

CLC Number:

Q336
S41-30

SONG Xijiao, XIE Li, XUAN Yuji, WANG Fang, YAN Chengqi, CHEN Jianping. Pathogenic diagnosis of synergistic infection of potato mosaic disease[J]. , 2018, 30(1): 99-105.

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Pathogenic diagnosis of synergistic infection of potato mosaic disease

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