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    Crop Science
    Comparison of fitting models of light response curve of potato under different light quality
    XU Jianmin, SHI Hedi, SHI Peihua, ZHANG Zeyang, XU Zhigang
    2020, 32(5):  753-761.  DOI: 10.3969/j.issn.1004-1524.2020.05.01
    Abstract ( 495 )   HTML ( 370 )   PDF (1316KB) ( 1725 )  
    In order to study the photosynthetic capacity, light response curve and applicability of data fitting models of potatoes under different light quality treatments, the test was conducted with potato plantlets grown in pot under blue light (B), red light (R), B∶R=1∶3(B1R3), B∶R=1∶5(B1R5), B∶R=1∶7(B1R7) light spectrum to observe photosynthetic pigments, photosynthesis and light response curve of potato. The exponential model, rectangular hyperbolic model, non-rectangular hyperbolic model, Ye modified and modified exponential model were used to fitting light response curve, calculating photosynthetic parameters of potato under different light quality treatments. The results showed that the photosynthetic pigment content and photosynthetic capacity under the B1R7 treatment were the highest among the five treatments, the Ye modified and modified exponential model were better than the exponential model, rectangular hyperbolic model, non-rectangular hyperbolic model, especially in fitting Pnmax, LSP and Rd. The light compensation point, light saturation point and maximum net photosynthetic rate of potato under B1R7 treatment were the highest among the five treatments. The light quality combination of B1R7 could enhance photosynthesis of potato, and Ye modified and modified exponential model were more suitable for fitting potato light response curve.
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    Animal Science
    Prokaryotic expression and purification of PEDV N protein and analysis of B cell antigen epitope
    GUO Fucheng, JIN Li, SU Qiang, SU Yuxin, LI Fanglin, CHEN Shien, MA Xiaoxia
    2020, 32(5):  762-769.  DOI: 10.3969/j.issn.1004-1524.2020.05.02
    Abstract ( 642 )   HTML ( 3 )   PDF (1782KB) ( 1676 )  
    The aim of this study was to clone and construct the porcine epidemic diarrhea virus (PEDV) N gene and to predict the epitope properties of its B cells. The N gene of PEDV was amplified by PCR and cloned into the prokaryotic expression vector pET-28a(+).The positive clone was obtained by enzyme digestion, and the positive clone was expressed in the expression strain E. coli BL21 (DE3). High purity expressed proteins were obtained by optimizing expression conditions and purification conditions. The 3D structure of PEDV-N protein was established by bioinformatics homology modeling software Swiss-Pdb Viewer and the secondary structure, antigenicity, hydrophilicity and surface probability of PEDV-N protein were predicted according to bioinformatics software DNA-Star, and the antigen epitopes of its B cells were predicted by comprehensive analysis. It is predicted that there were 13 B cell dominant epitopes in the amino acid sequence of PEDV-N protein. The research results will further provide a theoretical basis for the application of PEDV-N protein expression products in vitro and the development of genetically engineered vaccines.
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    Effect of heat stress on gene expressions and signaling pathways of mammary gland in Chinese Holstein
    LI Qiuling, QI Ying, WANG Chen, ZHANG Yiming, WANG Xinyu, SHANG Xiaolan, JIA Yonghong, LI Meiru, CHU Mingxing
    2020, 32(5):  770-778.  DOI: 10.3969/j.issn.1004-1524.2020.05.03
    Abstract ( 846 )   HTML ( 3 )   PDF (2094KB) ( 1720 )  
    Heat stress caused great economic loss to dairy production in summer. To reveal the molecular mechanism of mammary gland response to heat stress, differentially expressed genes were studied to explore the effect of differentially expressed genes on transcription regulation of mammary tissues in dairy cows. Four heat stressed (August, Temperature humidity index, THI=83.8) and 4 non-heat stressed (March, THI=65.8) mammary glands of Chinese Holstein were sampled, sequenced by HiSeq2000 and analyzed by comprehensive bioinformatics. There were 96 differentially expressed genes (fold change>2) obtained, among which 46 genes were up-regulated and 50 genes were down-regulated. GO functional annotation results showed that there were 41, 38 and 36 genes in cell components, molecular functions, and biological processes, respectively (P<0.05). KEGG was enriched in 18 pathways (P<0.05), including 6 pathways related to diseases, 9 pathways related to metabolisms, immune response related cytokine-cytokine receptor interaction, and NOD-like receptor signaling pathway. The transcriptome of mammary gland in heat stressed and non-heat stressed Chinese Holstein were compared, and the related signaling pathways were analyzed, which may provide a theoretical basis for understanding the molecular mechanisms of heat stress in dairy cattle.
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    Prokaryotic expression of porcine type Ⅲ interferon and studying on its antiviral activity
    CHEN Yunlu, SHAN Ying, LUO Hao, XU Jidong, ZHAO Lingyan, FANG Weihuan, LI Xiaoliang
    2020, 32(5):  779-788.  DOI: 10.3969/j.issn.1004-1524.2020.05.04
    Abstract ( 978 )   HTML ( 4 )   PDF (3543KB) ( 1648 )  
    Normally, type III interferon (IFN-λ) was highly expressed in intestinal mucosa and other mucosal systems, with relatively stronger broad-spectrum antiviral ability and immune regulating ability. Porcine epidemic diarrhea virus (PEDV) and porcine deltacorona virus (PDCoV) were two major intestinal pathogens causing diarrhea in piglets, which hindered the swine industry severely. In this study, the recombinant plasmid containing PoIFN-λ3 fragment was constructed. The recombinant plasmid was then transformed into E.coli Rosaetta cells for recombinant expression. Analyzed by SDS-PAGE and western blotting, the results showed that the recombinant PoIFN-λ3 (27 ku) was successfully expressed in the inclusion bodies of E. coli cells. After denaturation, purification and renaturation, the active PoIFN-λ3 recombinant protein was obtained and was used to treat IPEC-J2 cells. Challenged by recombinant PoIFN-λ3, the immune-stimulating genes (ISGs), such as ISG-15, OAS1, Mx-1, IFIT1, IFITM1 and IFITM3 were all up-regulated significantly and reached to the peak (P<0.001) at 12 h post challenge. For PEDV and PDCoV infection in IPEC-J2, the virus replication was detected after pretreatment, simultaneous treatment and post-infection treatment of recombinant PoIFN-λ3. Compared with the control, the viral copy numbers of both PEDV and PDCoV were decreased significantly (P<0.05) after treatment of recombinant PoIFN-λ3. The above results indicated that the purified and renatured PoIFN-λ3 recombinant protein could have good biological activity. The recombinant PoIFN-λ3 can induce high expression of different ISGs in IPEC-J2, and can also inhibit PEDV and PDCoV replication in the host cells. In summary, our study provided a basis for preventing and treating viral diarrhea in piglets.
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    Prokaryotic expression and phylogenetic analysis of ORF2 gene of goose astrovirus
    PU Lusha, SU Shibo, CHEN Xiaohan, ZHAO Lili, CHEN Hongyan
    2020, 32(5):  789-797.  DOI: 10.3969/j.issn.1004-1524.2020.05.05
    Abstract ( 811 )   HTML ( 5 )   PDF (1481KB) ( 1679 )  
    The goose astrovirus (GoAstV) ORF2 gene was amplified by RT-PCR, and the amplified product was cloned into the prokaryotic expression vector pET32a expressed in E.coli BL21. SDS-PAGE analysis showed that the ORF2 protein with the size of 106 ku was obtained after the induction of recombinant bacteria, which was consistent with the theoretical value. The polyclonal antibody was prepared by immunizing mice with the induced protein that was purified by Ni column affinity chromatography and the antibody titer was determined. Western blotting results showed that ORF2 protein could be recognized by mouse anti GoAstv positive serum. Sequence analysis showed that the nucleotide similarity between the virus and other astrovirus of goose origin were between 37.8% and 99.3%. The HLJ-1801 isolate was located in the same branch with the eight published strains of astrovirus of goose origin, indicating that it belonged to a new type of astrovirus. In this study, the GoAstV ORF2 protein was successfully and efficiently expressed using a prokaryotic expression system, and the fusion protein existed as an inclusion body. The purified ORF2 recombinant protein and mouse anti goastv polyclonal antibody were successfully obtained, which laid a foundation for further research on the diagnostic reagent of ORF2 protein for astrovirus infection.
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    Effects of microRNA-499 on lipid metabolism-related gene expression in Pelodiscus sinensis
    LU Yi, GAO Youling, WANG Shuitao, HE Shengsheng
    2020, 32(5):  798-803.  DOI: 10.3969/j.issn.1004-1524.2020.05.06
    Abstract ( 657 )   HTML ( 3 )   PDF (1147KB) ( 1699 )  
    In this study, a specific inhibitor antagomirs of microRNA-499(miR-499) was injected to reveal the regulatory effect of microRNA-499 on lipid metabolism related genes of Pelodiscus sinensis. Fifteen healthy juvenile Pelodiscus sinensis were selected and randomly divided into control group, saline group and miR-499 antagomirs group, respectively. After 3 days of continuous injection at the same time, triglyceride and total cholesterol in serum and liver were determined, and the expression levels of miR-499 and lipid metabolism related genes in liver were determined by real-time quantitative PCR. The results showed that the injection of miR-499 antagomirs increased the expression levels of thyroid hormone response protein gene(THRSP) and sodium/iodine co-transporter gene(NIS), and decreased the expression level of carnitine palmitoyl transferase 1 gene(CPT1) compared with the control group and the saline group (P<0.05). The injection of miR-499 antagomirs affected the expression level of lipid metabolism genes, and miR-499 was related to fat production and fatty acid β-oxidation of Pelodiscus sinensis.
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    Horticultural Science
    Inhibition of phosphorus deficiency on cadmium accumulation in Arabidopsis thaliana
    ZHU Senlin, MEI Zhong, XING Chenghua
    2020, 32(5):  804-809.  DOI: 10.3969/j.issn.1004-1524.2020.05.07
    Abstract ( 716 )   HTML ( 3 )   PDF (1045KB) ( 1617 )  
    Wild-type Arabidopsis thaliana Col-0 and iron transporter deletion mutant irt1 were used as experimental materials to study the mechanism of phosphorus deficiency. The following tests were applied: phosphorus-free plus cadmium-free treatment (-P-Cd), phosphorus-free plus cadmium treatment (-P+Cd), normal phosphorus supply without cadmium treatment (+P-Cd) and normal phosphorus supply plus cadmium treatment (+P+Cd). The results showed that under the condition of 40 μmol·L-1 cadmium treatment, phosphorus deficiency treatment significantly raised relative leaf chlorophyll content and dry weight of wild-type Arabidopsis thaliana Col-0, and attenuated the toxicity of cadmium to wild-type Arabidopsis thaliana Col-0 compared with normal phosphorus supply. At the same time, phosphorus deficiency treatment reduced the cadmium content in Col-0 plants and inhibited the expression of iron transporter 1 (IRT1) in roots. Phosphorus deficiency treatment in the irt1 mutant also reduced cadmium content in plants, but the reduction was significantly lower than the results obtained for Col-0. Therefore, IRT1 gene played a role in inhibiting cadmium uptake and relieving cadmium toxicity.
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    Genetic diversity analysis of Sabia parviflora based on chloroplast gene psbA-trnH sequence
    YAN Fulin, WANG Bo, WEN Di, XU Wenfen, SUN Qingwen, WEI Shenghua
    2020, 32(5):  810-815.  DOI: 10.3969/j.issn.1004-1524.2020.05.08
    Abstract ( 777 )   HTML ( 3 )   PDF (1043KB) ( 1661 )  
    System evolution relationship, genetic diversity and molecular identification method of the germplasm resources of Sabia parviflora from different regions of Guizhou were analyzed based on the sequence of psbA-trnH chloroplast gene. The psbA-trnH sequences of 13 Sabia parviflora samples from 4 counties and districts in Guizhou were amplified and sequenced by PCR. Codon Code Aligner 8.0.2 software was applied for collation and splicing, and population genetic distance, haplotype number and diversity were analyzed to construct phylogenetic tree. The length of psbA-trnH sequence of 13 chloroplast genes of Sabia parviflora was 469-488 bp, with 37 polymorphism sites, 14 reduced information sites, 23 indigenous sites, and 3 inserted/deleted fragments. The variation range of genetic distance between different populations was 0-0.085 9, and the average genetic distance was 0.025 3, with 4 haplotypes, haplotype (gene) diversity (Hd) of 0.679. In Fu and Li's D* test, Fu and Li's F* test, and Tajima's D test, P values were all greater than 0.10, without statistical significance. In conclusion, the evolution rate of Sabia parviflora was not consistent, and the genetic diversity was rich. This study enriched the research data of Sabia parviflora and its genera, and provided a certain reference for the classification, identification, systematic evolution and molecular marker development of Sabia parviflora.
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    Cadmium tolerance mechanism of Solanum nigrum L. based on components and enzyme activity changes in apoplast
    WANG Xiaoming, CHEN Ailing, DONG Lu, SHI Yujun, ZHENG Liuchang, ZHAO Lianhui, ZHANG Ruiqi, ZHANG Fenqin
    2020, 32(5):  816-823.  DOI: 10.3969/j.issn.1004-1524.2020.05.09
    Abstract ( 576 )   HTML ( 3 )   PDF (2565KB) ( 1581 )  
    To investigate the cadmium tolerance mechanism of Solanum nigrum L., a substrate culture experiment was conducted to study the effects of 50 μmol·L-1 Cd (CdCl2·2.5H2O) treatment on the contents of pectin and lignin, the activities of polygalacturonase (PG), cellulase (Cx), NADH-peroxidase (NADH-POD), guaiacol peroxidase (GPOD) and coniferylalcohol peroxidase (CAPX) in cell wall, and the content of hydrogen peroxide (H2O2) and the activities of superoxide dismutase (SOD), catalase (CAT), ascorbic acid oxidase (APX) in apoplast sap. The results showed that after 50 μmol·L-1 Cd treatment for 120 days, compared with the control, the length of shoot and root were inhibited, and the biomass of root was decreased (P<0.05), but the leaf area, chlorophyll content (SPAD), net photosynthetic rate (Pn) and intercellular CO2 concentration (Ci) showed no significant difference (P>0.05). In the cell wall of leaves, the activity of NADH-POD, GPOD and CAPX and the pectin content were all significantly increased (P<0.05), the PG activity was decreased (P<0.05), and Cx activity and lignin content showed no significant difference (P>0.05). In the apoplast sap of leaves, H2O2 content increased significantly (P<0.05), and the activity and isozyme band of GPOD and SOD weren't significantly influenced (P>0.05), but the activities of APX and CAT increased (P<0.05) with more isozyme band number and enhanced isozyme band, respectively. In conclusion, the pectin, H2O2-producing NADH-POD and H2O2-consuming GPOD in cell wall, and H2O2-consuming APX and CAT in apoplast sap might play major roles in Cd tolerance of Solanum nigrum L.
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    Analysis on genetic relationship of mango (Mangifera indica L.) germplasms by CDDP markers
    ZHANG Yu, ZHANG Ji, RONG Tao, OU Kewei, HUANG Guodi, SONG Hongxia
    2020, 32(5):  824-830.  DOI: 10.3969/j.issn.1004-1524.2020.05.10
    Abstract ( 691 )   HTML ( 1 )   PDF (1214KB) ( 1744 )  
    To analyze the genetic diversity of 31 mango varieties and provide theoretical basis for the identification of mango germplasm resources and molecular assisted breeding, the genetic diversity of 31 mango varieties preserved in Guangxi Subtropical Crops Research Institute was analyzed by conserved DNA-derived polymorphism(CDDP) molecular marker technique. Eight CDDP primers were selected for PCR amplification of mango species tested, electrophoresis, cluster analysis and principal component analysis. There were 107 clear bands amplified from 31 mango varieties, with number of polymorphic bands (NPB) of 100 and polymorphism rate (PPB) of 93.57%. On average, 13 bands and 12 polymorphism bands were amplified from each primer. The average allele number (Na), effective allele number (Ne), Nei's gene diversity (H) and Shannon information index (I) of each locus were 1.847 1, 1.636 8, 0.410 8 and 0.84, respectively, indicating that there was rich genetic diversity among mango species. The range of similarity coefficients between mango species was 0.620 6-0.920 8. UPGMA clustering results showed that mango could be divided into 7 categories at the genetic similarity coefficient of 0.690 6. The results of principal component analysis were basically consistent with the results of cluster analysis, and the classification results were not closely related to the mango origin. The CDDP primer obtained by screening had a high polymorphism detection rate on mango germplasm resources, which was suitable for resource identification and relationship analysis.
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    Plant Protection
    Identification of antagonistic bacteria Bacillus velezensis JK3 against anthracnose of strawberry and its antipathogenic activity
    FENG Jiangpeng, QIU Liping, LIANG Xiuyan, CHEN Bixiu, XIA Haiyang, PENG Chunlong, ZHONG Yongjun
    2020, 32(5):  831-839.  DOI: 10.3969/j.issn.1004-1524.2020.05.11
    Abstract ( 652 )   HTML ( 9 )   PDF (2218KB) ( 1729 )  
    A bacterial isolate JK3, which was isolated from compost, showed stronger antagonistic activity against Colletotrichum gloeosporioides causing strawberry anthracnose in dual culture assay. The isolate JK3 was identified as Bacillus velezensis by physiological and biochemical characterization, 16S rDNA and rpoB gene sequence analysis. The inhibitory effects of JK3 fermented liquid on C. gloeosporioides were evaluated with the detached strawberry leaves. JK3 fermented liquid showed significant protection on strawberry anthracnose but poor therapeutic effect. More than 95% control efficiency was achieved by spraying JK3 fermented liquid 24 h before inoculation with the spore suspension of C. gloeosporioides or simultaneous inoculation. Only 11.5% control efficiency was resulted by spraying JK3 fermented liquid 24 h after inoculation with the pathogen. The antagonistic activity of the fermented liquid was stable to different pH values and proteases, but sensitive to high temperature. Thus implied that active component was not related to proteins. It has been reported that most isolates of Bacillus spp. produce active lipopeptides. The existences of lipopeptide biosynthetic genes in JK3 were examined by PCR amplification with proper primer pairs and DNA sequencing. Results showed that the JK3 contained srfAA, bymB, fenD, bacA and ituC genes. The above data indicated that B. velezensis JK3 had a potential to be developed as biocontrol agent for controlling strawberry anthracnose caused by C. gloeosporioides.
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    Screening, identification of Phytophthora infestans and its antagonistic bacterial strain
    LIU Ziying, YUAN Bin, XIAO Huamei, WU Yongfei, LIU Xiaolin, HU Xiangfei
    2020, 32(5):  840-848.  DOI: 10.3969/j.issn.1004-1524.2020.05.12
    Abstract ( 947 )   HTML ( 8 )   PDF (3755KB) ( 1635 )  
    Phytophthora infestans was identified from potato via tissue isolation method and the antibacterial effects of three antagonistic bacterium to it were compared in order to get the potential one. They were isolated by coating method and identified by morphology and molecular biology method. The inhibitory effects of Phytophthora infestans were studied including the release of zoospore, germination of resting spore and germination of sporangium. The preventive effect of the bacterial solution was also analyzed. The results showed that a PLB-2 Phytophthora infestans and a WZ-502 Brachybacterium sp. of potato were separated and identified. The strain WZ-502 had significant inhibitory effect on the release of zoospores, direct germination of sporangium and germination of resting spores. The strain WZ-502 (concentration was 3.65×107 CFU·mL-1) at dilutions grade of 10-2-10-3 was effective, and the rates of sporangium germination and the release of Phytophthora infestans were both below 40% when the dilution was 10-3. The strain WZ-502 had a good antibacterial effect on Phytophthora infestans.
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    Transcriptome analysis on heat tolerance of Chilo suppressalis larvae
    ZHU Yu, LIU Yang
    2020, 32(5):  849-857.  DOI: 10.3969/j.issn.1004-1524.2020.05.13
    Abstract ( 770 )   HTML ( 5 )   PDF (1807KB) ( 1454 )  
    Chilo suppressalis has strong adaptability to temperature. In high temperature environments, C. suppressalis larvae can produce antioxidant substances such as heat shock proteins, superoxide dismutase and catalase to eliminate the damage caused by oxidative stress. To reveal the effect of short-term high temperature on C. suppressalis larvae, a comparative transcriptome analysis between two sets of C. suppressalis larvae, treated at 38 ℃ and 26 ℃ for two hours respectively, was performed. The transcriptome results showed that the clean reads of all samples mapped to the reference genome accounted for more than 89%, the percentage of the sequences with unique mapped was higher than 82%, and the percentage of the sequences with multiple positions did not exceed 9%. Through differential expression analysis, 387 differentially expressed genes were obtained. Among them, heat shock protein and cytochrome P450 were up-regulated expression of genes related to the response to heat stress. KEGG enrichment analysis was performed on the up-regulated differentially expressed genes, and the four pathways in the protein processing in endoplasmic reticulum, longevity regulating pathway-multiple species, plant-pathogen interaction and antigen processing and presentation were significantly enriched. This study revealed the overall expression levels of genes involved in heat stress response in the larvae of C. suppressalis, and provided references for related experiments on heat stress response of C. suppressalis.
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    Screening and identification of antagonistic strains of wolfberry root rot
    ZHANG Xiaoyan, HE Jing, HOU Caixia, ZHANG Shuheng
    2020, 32(5):  858-865.  DOI: 10.3969/j.issn.1004-1524.2020.05.14
    Abstract ( 795 )   HTML ( 11 )   PDF (2498KB) ( 1529 )  
    Wolfberry diseases and insect pests are important factors affecting the development of wolfberry industry, among which the root rot of wolfberry is a common disease. In this study, healthy wolfberry rhizosphere soil samples were selected from seriously diseased wolfberry plantations and strains were isolated by conventional dilution method. Fusarium solani, the main pathogen of wolfberry root rot, was selected as the tested strain by plate confrontation method, and the antagonistic strain was identified by morphology and molecular biology. The results showed that 101 strains of bacteria, 52 strains of actinomycetes, 30 strains of mycetes and 27 strains of saccharomycetes were isolated from rhizosphere soil samples of healthy wolfberry. Two antagonistic strains, HYM-19 and HYC-7, were screened and identified as Sinorhizobium sp. and Metarhizium sp., respectively. The results provided a theoretical basis for the effective use of soil microorganisms to manage root rot of wolfberry.
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    Environmental Science
    Analysis into influencing factors of cultivated land productivity in Gao'an City based on structural equation model
    LI Zilin, HAN Yi, GUO Xi, GUO Jiaxin, LIN Wenlu
    2020, 32(5):  866-877.  DOI: 10.3969/j.issn.1004-1524.2020.05.15
    Abstract ( 569 )   HTML ( 3 )   PDF (3002KB) ( 1574 )  
    In the present study, Gao'an City was selected as the research area. Based on 147 monitoring points, with cultivated land productivity, soil nutrients, farmland water conservancy, geological characters and soil properties as latent variables in the model, the structural equation modeling (SEM) was adopted to construct the influencing factors model of cultivated land productivity, and the main factors affecting the cultivated land productivity in Gao'an were discussed. The results showed that soil nutrients, farmland water conservancy and soil properties were the main influencing factors affecting cultivated land productivity, with direct or indirect positive effects. The degree of direct influence was ranked as soil nutrient (0.55)>soil properties (0.26)>farmland water conservancy (0.23). Geological characters had no direct impact on cultivated land productivity, but had indirectly impact by affecting soil nutrients. Slope was the only variable with negative effect on cultivated land productivity. The cultivated land productivity in Gao'an City was affected by many factors. Thus, it was recommended to achieve coordinated and sustainable regional food production by improving water conservancy facilities, strengthening the construction of farmland quality, as well as improving the quality of agricultural products to build the healthy productivity of cultivated land.
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    Vertical distribution of soil organic carbon and dissolved organic carbon in reddish paddy soil under long-term green manure utilization
    XIA Wenjian, QIN Wenjing, LIU Jia, CHEN Xiaofen, ZHANG Lifang, CAO Weidong, XU Changxu, CHEN Jingrui
    2020, 32(5):  878-885.  DOI: 10.3969/j.issn.1004-1524.2020.05.16
    Abstract ( 1337 )   HTML ( 2 )   PDF (1080KB) ( 1735 )  
    The objective of this study was to evaluate the effects of long-term application of green manure on the distribution of soil organic carbon (SOC) and dissolved organic carbon (DOC) in reddish paddy soil. Soil samples were collected from the 8 years' long-term experiments located in Fengcheng City, Jiangxi Province. Four treatments were carried out in the experiment: CK (no fertilizer), F (only chemical fertilizers), M (only green manure), F+M (green manure plus chemical fertilizers). The green manure was Chinese milk vetch (Astragalus sinicus L.). The vertical distribution of SOC and DOC in soil layers and the quantitative relationship of SOC and DOC were investigated. The results showed that, the variation of SOC and DOC contents with soil depths could be described by S-function in early rice and late rice season. The determination coefficients of function equations were 0.928-0.966 and 0.876-0.975, which reached significant level at P<0.01. The application of chemical fertilizers or green manure (treatment F, M, and F+M) affected SOC and DOC contents in the topsoil (0-20 cm). There was no significant difference in SOC and DOC in deep soil (>20 cm) within different treatments. Green manure treatments (F+M and M) significantly (P<0.05) increased SOC and DOC in topsoil, while chemical fertilizers treatment (F) only significantly (P<0.05) increased the SOC content in topsoil. Compared with CK, the contents of SOC in topsoil treated with M, F and F+M in early rice season were significantly (P<0.05) increased by 51.1%, 80.9%, 92.8%, respectively, and the contents of DOC in topsoil treated with M and F+M in early rice season were significantly (P<0.05) increased by 12.9% and 46.2%, respectively; while the contents of SOC and DOC in topsoil treated with M, F and F+M in late rice season were significantly (P<0.05) increased by 66.6%, 81.3%, 81.2% and 37.1%, 10.8%, 45.2%, respectively. The DOC content of topsoil increased linearly with SOC, and the increase ratio of DOC with SOC in late rice season was higher than that in early rice season. The long-term application of chemical fertilizers reduced DOC/SOC, yet green manure could mitigate DOC/SOC decrease. Green manure could not only increase soil organic carbon, but also benefit the stability of soil organic carbon ecological function.
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    Biosystems Engineering
    Tea plant growth monitoring based on time series cruise images
    LIU Lianzhong, LI Mengjie, NING Jingming
    2020, 32(5):  886-896.  DOI: 10.3969/j.issn.1004-1524.2020.05.17
    Abstract ( 577 )   HTML ( 3 )   PDF (4644KB) ( 1625 )  
    The growth of tea plant is easily affected by many factors, such as soil conditions, diseases and insect pests, pruning and picking, it is necessary to monitor the growth of tea plant. However, manpower monitoring is time-consuming, laborious and inefficient. In order to solve this problem, a tea plant growth image monitoring system was designed in this paper, which could monitor tea plant in real time. It was helpful to find out the abnormal situation of tea plant growth in time, and take effective measures to reduce economic losses and improve the quality and yield of tea. In the proposed design, a zoom camera was set up at suitable position, and the tea plantation was divided into several monitoring regions. The video flow of the monitoring region was obtained by zoom and rotation of the camera to accomplish real-time tea plant monitoring. Images of each monitoring region were captured in sequence and saved into image database of tea plant growth according to the monitoring region and time. By analyzing the image database, growth information such as the germination of tea buds, occurrence process of diseases, nutrition, and environmental information such as light, weather change, was obtained to understand the growth of tea plant in a more comprehensive way. Nitrogen diagnose method was also proposed to get real-time nutrition of tea plant. Through regression analysis between color parameters and nitrogen content, a fast monitoring model of nitrogen content of tea plant was obtained, with mean relative error less than 6%. The design provided a fast, simple and economical mean to obtain growth information from tea plant and other crops.
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    Optimization of wireless sensor network for water quality monitoring based on improved cuckoo search algorithm
    HU Jian, HU Fengjun, ZHANG Hong, ZHU Ying
    2020, 32(5):  897-903.  DOI: 10.3969/j.issn.1004-1524.2020.05.18
    Abstract ( 450 )   HTML ( 2 )   PDF (2331KB) ( 1406 )  
    In order to solve the problem of uneven distribution of random deployment of traditional sensor networks, cuckoo search algorithm (CS) was proposed to optimize node deployment. In order to improve the global optimization performance of CS algorithm and enhance the deployment optimization ability of sensor nodes, inspired by gradient descent with momentum, root mean square algorithm and Adam optimization algorithm, three improved algorithms, namely Momentum-CS, RMSprop-CS and Adam-CS, were proposed to optimize and adjust the step size control and elimination probability in CS algorithm. Taking network coverage as the optimization target, three algorithms were used to optimize the deployment of water quality monitoring wireless sensor nodes in water area with length and width of 100 meters. Simulation results showed that the Adam-CS algorithm could achieve a higher network coverage of 90.35% in fewer iterations, which was of practical significance for guiding the deployment of wireless sensor nodes in water environment monitoring.
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    Improvement of methane production potential by post-treatment of vinegar residue with Phanerochaete chrysosporium
    LI Qian, XU Zhiyang, RUAN Wenquan
    2020, 32(5):  904-911.  DOI: 10.3969/j.issn.1004-1524.2020.05.19
    Abstract ( 506 )   HTML ( 2 )   PDF (2029KB) ( 1410 )  
    Delignification is the key to improve the anaerobic digestion performance of vinegar residue. In this study, vinegar residue after anaerobic fermentation was used as substrate. The delignification was realized by post-treatment of vinegar residue with Phanerochaete chrysosporium, so as to deeply improve the methane production potential. The degradation and methane production potential of vinegar residue were investigated under different post-treatments (inoculation amount and post-treatment time). It was shown that increasing the inoculum amount and prolonging the post-treatment time could promote the secretion of lignocellulase, so as to obtain higher degradation effect. When the inoculation amount was 9 pieces and the post-treatment time was 16 days (experimental group T9-16), the substrate degradation efficiency was the highest. The degradation rates of total solids and violate solids were 8.96% and 9.91%, respectively. And the degradation rates of cellulose, hemicellulose and lignin reached 7.84%, 23.72% and 11.84%, respectively. Further experiments on the methane production potential showed that the biodegradability of vinegar residue was improved effectively by the post-treatment with P. chrysosporium. The optimal methane production was achieved in the experimental group T6-16 with 6 pieces of inoculation amount and 16 days of post-treatment time. The methane production per unit substrate (based on violate solids) of T6-16 reached up to 246.8 mL·g-1, which was 2.82 times of that in the control group (vinegar residue without post-treatment). This study confirmed the feasibility of post-treatment with P. chrysosporium in promoting the anaerobic digestion efficiency of vinegar residue.
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    Agricultural Economy and Development
    Inter-provincial correlation of agricultural carbon emission from perspectives of space, economy and technology
    HE Yanqiu, XU Jie, ZHU Siyu, CHEN Rou, DAI Xiaowen
    2020, 32(5):  912-922.  DOI: 10.3969/j.issn.1004-1524.2020.05.20
    Abstract ( 472 )   HTML ( 2 )   PDF (1102KB) ( 1460 )  
    Research on inter-provincial correlation of agricultural carbon emissions could lay foundation for the regional synergies, and benefit the realization of dual goals of green agriculture development and carbon emission reduction. According to the basic framework of the national greenhouse gas emissions inventory guidelines, agricultural carbon emissions were measured comprehensively in five sectors, namely, agricultural energy, farmland use, ruminant feeding, crop planting and agricultural waste. Regional correlation of carbon emissions were verified by spatial statistical analysis, and the possible reasons were explored from the perspectives of spatial adjacency, economic dependence and technological association by nonparametric QAP regression. Finally, countermeasures were proposed including optimizing energy layout, improving energy efficiency; focusing on the diffusion effect of emission reduction technologies; formulating differentiated emission reduction measures by sub-region and sub-sector; establishing “four principles, three cores” regional synergistic emission reduction mechanism.
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    Spatial and temporal analysis into effect of cultivated land use in grain production in Fujian Province
    HUANG Jindong
    2020, 32(5):  923-930.  DOI: 10.3969/j.issn.1004-1524.2020.05.21
    Abstract ( 536 )   HTML ( 2 )   PDF (1187KB) ( 1515 )  
    The present study took Fujian Province as the research area, and analyzed the grain production and cultivated land use at both province and city levels. The LMDI model was used to decompose the contribution of cultivated land use factors to grain production, and the spatial and temporal analysis of cultivated land use effect was carried out. The results showed that the grain output in Fujian Province was decreased by about 2.4% in 2009-2016, and the grain farming ratio dropped by 8.8%. The decline of grain farming ratio was the main cause of reduction of grain production. From 2009 to 2016, only Longyan and Sanming achieved grain production growth among the cities in Fujian Province. The per capita arable land area of Xiamen's agricultural labor force experienced the largest decline. The grain yield per hectare in Longyan and Zhangzhou increased. Quanzhou's agricultural labor force fell the most. And the grain farming ratio in all cities in Fujian Province declined. Structure effect was the main reason for the reduction of grain production in Fujian, and the factors affecting the change of grain production in different cities were different.
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    Technological innovation empowered by ownership structure optimization:an empirical study based on panel data of Zhejiang agricultural leading enterprises
    YU Bin, WANG Weifang
    2020, 32(5):  931-940.  DOI: 10.3969/j.issn.1004-1524.2020.05.22
    Abstract ( 746 )   HTML ( 2128896025 )   PDF (1019KB) ( 1723 )  
    Technological innovation has been considered as the key element for the sustainable and healthy development of Chinese economy in the second half. The improvement of technological innovation level of agricultural leading enterprises will help to realize high-quality development in agriculture and rural revitalization. Based on the theory of corporate governance, the survey of agricultural leading enterprises and the data of listed agricultural companies since 2012 in Zhejiang Province, the present study mainly discussed the effect of ownership concentration degree, ownership balance degree, enterprise nature, managerial ownership and institutional investor ownership on the technological innovation of enterprises by total sample and grouped samples regression analysis. It was shown that the relationship between ownership concentration degree and technological innovation was “N” type. The institutional investor ownership had a negative effect on technological innovation, and the relationships within ownership balance degree, enterprise nature, managerial ownership and technological innovation were dependent on the ownership concentration. It was expected to improve the ownership structure, promote the improvement of corporate governance mechanism, build a favorable institutional environment to empower enterprise technological innovation.
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