Transcriptome analysis on heat tolerance of Chilo suppressalis larvae

doi:10.3969/j.issn.1004-1524.2020.05.13

›› 2020, Vol. 32 ›› Issue (5): 849-857.DOI: 10.3969/j.issn.1004-1524.2020.05.13

• Plant Protection • Previous Articles Next Articles

Transcriptome analysis on heat tolerance of Chilo suppressalis larvae

ZHU Yu, LIU Yang

Wenzhou Vocational College of Science and Technology, Wenzhou 325006, China

Received:2019-10-21 Online:2020-05-25 Published:2020-05-29

Abstract

Abstract: Chilo suppressalis has strong adaptability to temperature. In high temperature environments, C. suppressalis larvae can produce antioxidant substances such as heat shock proteins, superoxide dismutase and catalase to eliminate the damage caused by oxidative stress. To reveal the effect of short-term high temperature on C. suppressalis larvae, a comparative transcriptome analysis between two sets of C. suppressalis larvae, treated at 38 ℃ and 26 ℃ for two hours respectively, was performed. The transcriptome results showed that the clean reads of all samples mapped to the reference genome accounted for more than 89%, the percentage of the sequences with unique mapped was higher than 82%, and the percentage of the sequences with multiple positions did not exceed 9%. Through differential expression analysis, 387 differentially expressed genes were obtained. Among them, heat shock protein and cytochrome P450 were up-regulated expression of genes related to the response to heat stress. KEGG enrichment analysis was performed on the up-regulated differentially expressed genes, and the four pathways in the protein processing in endoplasmic reticulum, longevity regulating pathway-multiple species, plant-pathogen interaction and antigen processing and presentation were significantly enriched. This study revealed the overall expression levels of genes involved in heat stress response in the larvae of C. suppressalis, and provided references for related experiments on heat stress response of C. suppressalis.

Key words: Chilo suppressalis, larvae, heat stress, transcriptome

CLC Number:

S433.4

ZHU Yu, LIU Yang. Transcriptome analysis on heat tolerance of Chilo suppressalis larvae[J]. , 2020, 32(5): 849-857.

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Transcriptome analysis on heat tolerance of Chilo suppressalis larvae

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