Effect of heat stress on gene expressions and signaling pathways of mammary gland in Chinese Holstein

doi:10.3969/j.issn.1004-1524.2020.05.03

›› 2020, Vol. 32 ›› Issue (5): 770-778.DOI: 10.3969/j.issn.1004-1524.2020.05.03

• Animal Science • Previous Articles Next Articles

Effect of heat stress on gene expressions and signaling pathways of mammary gland in Chinese Holstein

LI Qiuling¹, QI Ying¹, WANG Chen¹, ZHANG Yiming¹, WANG Xinyu¹, SHANG Xiaolan¹, JIA Yonghong¹, LI Meiru¹, CHU Mingxing²

1. Hebei Key Laboratory of Animal Diversity, College of Life Sciences, Langfang Normal University, Langfang Key Laboratory of Cell Engineering and Application, Langfang 065000, China;
2. Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2019-11-29 Online:2020-05-25 Published:2020-05-29

Abstract

Abstract: Heat stress caused great economic loss to dairy production in summer. To reveal the molecular mechanism of mammary gland response to heat stress, differentially expressed genes were studied to explore the effect of differentially expressed genes on transcription regulation of mammary tissues in dairy cows. Four heat stressed (August, Temperature humidity index, THI=83.8) and 4 non-heat stressed (March, THI=65.8) mammary glands of Chinese Holstein were sampled, sequenced by HiSeq2000 and analyzed by comprehensive bioinformatics. There were 96 differentially expressed genes (fold change>2) obtained, among which 46 genes were up-regulated and 50 genes were down-regulated. GO functional annotation results showed that there were 41, 38 and 36 genes in cell components, molecular functions, and biological processes, respectively (P<0.05). KEGG was enriched in 18 pathways (P<0.05), including 6 pathways related to diseases, 9 pathways related to metabolisms, immune response related cytokine-cytokine receptor interaction, and NOD-like receptor signaling pathway. The transcriptome of mammary gland in heat stressed and non-heat stressed Chinese Holstein were compared, and the related signaling pathways were analyzed, which may provide a theoretical basis for understanding the molecular mechanisms of heat stress in dairy cattle.

Key words: dairy cattle, heat stress, mammary gland, gene expression, signal pathway

CLC Number:

S823

LI Qiuling, QI Ying, WANG Chen, ZHANG Yiming, WANG Xinyu, SHANG Xiaolan, JIA Yonghong, LI Meiru, CHU Mingxing. Effect of heat stress on gene expressions and signaling pathways of mammary gland in Chinese Holstein[J]. , 2020, 32(5): 770-778.

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Effect of heat stress on gene expressions and signaling pathways of mammary gland in Chinese Holstein

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