Analysis of differential expression of TLR4-MyD88 independent pathway genes under resistin-induced bovine alveolar macrophages

doi:10.3969/j.issn.1004-1524.2019.03.05

›› 2019, Vol. 31 ›› Issue (3): 379-383.DOI: 10.3969/j.issn.1004-1524.2019.03.05

• Animal Science • Previous Articles Next Articles

Analysis of differential expression of TLR4-MyD88 independent pathway genes under resistin-induced bovine alveolar macrophages

YANG Mingxian, ZUO Zhicai^*, LI Bi, WANG Yu

Key Laboratory of Animal Disease and Human Health of Sichuan Province, Key Laboratory of Environmental Hazard and Animal Disease of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received:2018-06-12 Online:2019-03-25 Published:2019-04-08

Abstract

Abstract: In order to explore the mechanism of bovine resistin through TLR4-MyD88 independent signal pathway in bovine alveolar macrophages, bovine alveolar macrophages were exposed to resistin(100 ng·mL^-1) for 0, 1.5, 3.0, 6.0, 12.0, 24.0 h, respectively. The relative mRNA expression of TLR4-MyD88 independent signal pathway genes (TLR4, TRAM, NF-κB) were detected by qRT-PCR, the expression of proinflammatory cytokine IL-6, 1L-1β and TNF-α were tested by ELISA methods. The results showed that resistin(100 ng·mL^-1) sharply upregulated the expression of TLR4-MyD88 independent signal pathway genes at 6.0 h(P<0.01). The expression of related genes reached the highest at 12.0 h. The expression of proinflammatory cytokines IL-6, IL-1β and TNF-α significantly increased at 1.5 h (P<0.01). Through the signal transmission, a large number of proinflammatory cytokines were released, causing pulmonary inflammation.

Key words: resistin, bovine alveolar macrophages, TLR4-MyD88 inpendent signal pathway, proinflammatory cytokine

CLC Number:

S852
R563.1

YANG Mingxian, ZUO Zhicai, LI Bi, WANG Yu. Analysis of differential expression of TLR4-MyD88 independent pathway genes under resistin-induced bovine alveolar macrophages[J]. , 2019, 31(3): 379-383.

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Analysis of differential expression of TLR4-MyD88 independent pathway genes under resistin-induced bovine alveolar macrophages

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