Sequencing and analysis of several genes of a virulent duck enteritis virus strain ZJ2016

doi:10.3969/j.issn.1004-1524.2020.02.06

›› 2020, Vol. 32 ›› Issue (2): 226-233.DOI: 10.3969/j.issn.1004-1524.2020.02.06

• Animal Science • Previous Articles Next Articles

Sequencing and analysis of several genes of a virulent duck enteritis virus strain ZJ2016

ZHANG Hongli, HUANG Jing, LIU Xia, WU Yunhong, FENG Xiaoxiao, WU Xuejun, XU Hui^*

Zhejiang Animal Disease Control Center, Hangzhou 311199, China

Received:2019-08-27 Online:2020-02-25 Published:2020-03-13

Abstract

Abstract: In 2016, a duck enteritis virus (DEV) strain ZJ2016 was identified in a duck farm infected with DEV in Zhejiang province. The antigen and virulence-related genes of ZJ2016 strain sequencing analysis was conducted using second-generation sequencer. The nucleic acid and amino acid sequences of the major envelope glycoprotein genes gB, gC, gG, gH, gI, gK, gL, gM, gN and pathogenicity genes LORF11, UL2, UL12, UL41, UL47, US10 were analyzed and compared with those of the published viruses and vaccine strains (VAC strain, clone-03 strain). The results showed that the sequence of ZJ2016 strain envelope glycoprotein associated with vaccine immune protection antigen did not change significantly compared with that of vaccine strain. However, the gene structure and homology of LORF11, UL2, UL12, UL41, UL47 and US10 of ZJ2016 strain were significantly different from the amino acid sequence of vaccine strain, and the homology of other virulent strains in China, such as LH2011, CHv and CV, were higher than that of vaccine strain. The results showed that ZJ2016 had the genotype characteristics of the virulent strain.

Key words: duck enteritis, antigen, virulence-related genes, virulent strain, vaccine strain

CLC Number:

S852.65

ZHANG Hongli, HUANG Jing, LIU Xia, WU Yunhong, FENG Xiaoxiao, WU Xuejun, XU Hui. Sequencing and analysis of several genes of a virulent duck enteritis virus strain ZJ2016[J]. , 2020, 32(2): 226-233.

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Sequencing and analysis of several genes of a virulent duck enteritis virus strain ZJ2016

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