›› 2017, Vol. 29 ›› Issue (9): 1465-1473.DOI: 10.3969/j.issn.1004-1524.2017.09.07

• Animal Science • Previous Articles     Next Articles

ERIC-PCR typing and virulence-related genes detection of Campylobacter jejuni isolated from poultry in some areas of Sichuan Province

WANG Han, ZHANG Bo, MO Yaxia, LUO Zhengzhong, JIANG Sixun, SUN Chengliang, CAO Suizhong, YAO Xueping*   

  1. College of Veterinary Medicine, Sichuan Agricultural University, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China
  • Received:2017-03-15 Online:2017-09-20 Published:2017-09-27

Abstract: In order to investigate the genetic diversity and virulence related genes of Campylobacter jejuni in some areas of Sichuan, the samples of the cecum and anal swab collected from the chickens, ducks and quails from Chengdu, Ya'an and Meishan were isolated and identified for C. jejuni, and the ERIC-PCR typing and seventeen virulence-related genes were detected and analyzed. The results of isolation showed a 10.23% separation rate (48/469), and the isolation rate from chicken was higher than that from quail(P<0.05). ERIC-PCR cluster analysis showed that forty-eight strains of C. jejuni could be divided into six clusters, and there were sixteen species and thirty-one genotypes. The isolates from each farm were clustered and distributed in some species or cluster, while some isolates showed cross distribution in different regions and different farms. The carrying rate of fifteen virulence related genes was above 50%, and the carrying rate of virulence related genes of quail was higher than other animals. The results suggested that the C. jejuni isolates from poultry of Sichuan area possessed high carrying rate and high genetic diversity.

Key words: poultry, Campylobacter jejuni, ERIC-PCR typing, virulence-related genes

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