Effects of soy isoflavones on expression of IL-2, IL-4, TNF-α and INF-γ in male rats' spleen

doi:10.3969/j.issn.1004-1524.2017.09.06

›› 2017, Vol. 29 ›› Issue (9): 1458-1464.DOI: 10.3969/j.issn.1004-1524.2017.09.06

• Animal Science • Previous Articles Next Articles

Effects of soy isoflavones on expression of IL-2, IL-4, TNF-α and INF-γ in male rats' spleen

LI Like¹, LUO Qihui^{1, 2}, HUANG Chao¹, CHEN Xiaolin¹, CHEN Ping¹, LI Yifan¹, LIU Wentao^{1, 2}, CHEN Zhengli^{1, 2, *}

1. Laboratory of Animal Disease Model,College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received:2017-02-23 Online:2017-09-20 Published:2017-09-27

Abstract

Abstract: In the present study, effects of different dose of soy isoflavones (SIF) on splenic immune function in male rats were studied and mechanism was explained. Forty rats aged 6 weeks were randomly divided four groups fed with SIF 0 (control group),50 (low dose group),250 (medium dose group), 500 (high dose group) mg·kg^-1 body weight. Body weight were recorded every week, and at the end of experiment the rats' spleens were separated and weighed. The expression levels of IL-2, IL-4, TNF-α and INF-γ in rats spleen were detected by immunohistochemistry SABC method. The results showed that compared with control group, there were no significantly differences in spleen index among the groups. Splenic corpuscles in middle and high group were smaller than those in other groups with the lymphocytes number significantly decreased in red pulp. The expression of IL-2 in low dose group was significantly decreased, but rapidly increased in middle and high dose group with a dose-dependent. However, the expression levels of IL-4, TNF-α and INF-γ were all significantly reduced after treated by isoflavones. All above,even though high dose isoflavones can increase IL-2 level,the expressions of IL-4, TNF-α and INF-γ were inhibited with the dose of isoflavones increasing, which indicated SIF had potential immunosuppression to male rats and appeared dosage difference.

Key words: soy isoflavone, spleen, immune function, rats

CLC Number:

LI Like, LUO Qihui, HUANG Chao, CHEN Xiaolin, CHEN Ping, LI Yifan, LIU Wentao, CHEN Zhengli. Effects of soy isoflavones on expression of IL-2, IL-4, TNF-α and INF-γ in male rats' spleen[J]. , 2017, 29(9): 1458-1464.

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Effects of soy isoflavones on expression of IL-2, IL-4, TNF-α and INF-γ in male rats' spleen

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