Effects of root pruning on growth and expression of related genes in Poncirus trifoliata

doi:10.3969/j.issn.1004-1524.2021.02.10

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (2): 270-277.DOI: 10.3969/j.issn.1004-1524.2021.02.10

• Horticultural Science • Previous Articles Next Articles

Effects of root pruning on growth and expression of related genes in Poncirus trifoliata

HUANG Yongming(), SONG Fang, WANG Ce, YAO Jinglei, WANG Zhijing, HE Ligang, WU Liming, JIANG Yingchun^*()

Institute of Fruit and Tea, Hubei Academy of Agricultural Sciences, Wuhan 430064, China

Received:2020-05-18 Online:2021-02-25 Published:2021-02-25
Contact: JIANG Yingchun

Abstract

Abstract:

In this study, Poncirus trifoliata seedlings were chosen as the test materials. The effects of four different root pruning levels (no cutting, cutting off 1/3 taproot, cutting off 1/2 taproot and cutting off 2/3 taproot) on the plant growth, root architecture system and nutrient contents of Poncirus trifoliata seedlings were investigated by a repeated experiment for two years, and the expression of key genes involved in the lateral roots development was analyzed under root pruning treatments. The results showed that cutting off 1/2 taproot treatment enhanced the height, stem diameter, shoot and root fresh weights, and improved the root system architecture parameters (the number of lateral roots, the total root length, the root surface area and average length), and also increased the contents of K, P, Ca, B, Fe, Na and Zn in roots of Poncirus trifoliata seedlings. In addition, quantitative real-time PCR (qRT-PCR) revealed that the expression levels of lateral root development related genes HOX1 and CYP2 were induced, but IAA11 and IAA13 were reduced under root pruning treatments. Overall, the moderate root pruning might regulate the development of lateral roots and improve the root architecture through up-regulating of HOX1 and CYP2 genes, and down-regulating of IAA11 and IAA13 genes, thus leading to the improvement of nutrient absorption and growth of Poncirus trifoliata seedlings.

Key words: root pruning, Poncirus trifoliata, root system architecture, gene expression

CLC Number:

S666.9

HUANG Yongming, SONG Fang, WANG Ce, YAO Jinglei, WANG Zhijing, HE Ligang, WU Liming, JIANG Yingchun. Effects of root pruning on growth and expression of related genes in Poncirus trifoliata[J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 270-277.

Figures/Tables 6

References 36

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引物名称 Primer name	引物序列 Primer sequences(5'-3')
IAA11-F	TGTTCCACCAGCCAACAACT
IAA11-R	AGCCAAGAAACATGCCCCAT
IAA13-F	ACTGGGCTTGAGTCTTGGTG
IAA13-R	ACTAGGTCTCCTCCCAGCAG
CYP2-F	GTTCTTCGTCTGTACGGCCA
CYP2-R	GAGCTGGACCCCACTTTCTC
HOX1-F	AAGCCCAAGAAGCGGTATCC
HOX1-R	ATCAACGGTTGGCTCCCATT
eIF1α-F	CCCCAAAGAATCAGAAACCAT
eIF1α-R	TGCCGAGCATACGAAGGAC

引物名称 Primer name	引物序列 Primer sequences(5'-3')
IAA11-F	TGTTCCACCAGCCAACAACT
IAA11-R	AGCCAAGAAACATGCCCCAT
IAA13-F	ACTGGGCTTGAGTCTTGGTG
IAA13-R	ACTAGGTCTCCTCCCAGCAG
CYP2-F	GTTCTTCGTCTGTACGGCCA
CYP2-R	GAGCTGGACCCCACTTTCTC
HOX1-F	AAGCCCAAGAAGCGGTATCC
HOX1-R	ATCAACGGTTGGCTCCCATT
eIF1α-F	CCCCAAAGAATCAGAAACCAT
eIF1α-R	TGCCGAGCATACGAAGGAC

检测年份 Detection year	根系修剪处理 Root prunning treatments	株高 Plant height/cm	茎粗 Stem diameter/cm	鲜重Fresh weight/g
检测年份 Detection year	根系修剪处理 Root prunning treatments	株高 Plant height/cm	茎粗 Stem diameter/cm	地上部Shoot	根系Root
2018	RP₀	17.01±2.25 ab	0.28±0.03 ab	0.64±0.03 b	1.27±0.24 b
	RP₁	18.38±1.88 a	0.26±0.01 b	0.65±0.62 ab	1.11±0.91 ab
	RP₂	18.37±1.36 a	0.31±0.04 a	0.72±0.20 a	1.44±0.17 a
	RP₃	15.04±1.63 b	0.28±0.06 ab	0.48±0.09 b	0.84±0.10 c
2019	RP₀	16.15±2.15 bc	0.27±0.05 b	0.59±0.18 b	1.05±0.12 b
	RP₁	18.07±1.86 a	0.25±0.01 c	0.59±0.15 b	0.94±0.23 bc
	RP₂	18.23±1.45 a	0.31±0.04 a	0.65±0.18 a	1.15±0.13 a
	RP₃	15.48±1.80 c	0.28±0.02 ab	0.54±0.11 b	0.86±0.24 c

检测年份 Detection year	根系修剪处理 Root prunning treatments	株高 Plant height/cm	茎粗 Stem diameter/cm	鲜重Fresh weight/g
检测年份 Detection year	根系修剪处理 Root prunning treatments	株高 Plant height/cm	茎粗 Stem diameter/cm	地上部Shoot	根系Root
2018	RP₀	17.01±2.25 ab	0.28±0.03 ab	0.64±0.03 b	1.27±0.24 b
	RP₁	18.38±1.88 a	0.26±0.01 b	0.65±0.62 ab	1.11±0.91 ab
	RP₂	18.37±1.36 a	0.31±0.04 a	0.72±0.20 a	1.44±0.17 a
	RP₃	15.04±1.63 b	0.28±0.06 ab	0.48±0.09 b	0.84±0.10 c
2019	RP₀	16.15±2.15 bc	0.27±0.05 b	0.59±0.18 b	1.05±0.12 b
	RP₁	18.07±1.86 a	0.25±0.01 c	0.59±0.15 b	0.94±0.23 bc
	RP₂	18.23±1.45 a	0.31±0.04 a	0.65±0.18 a	1.15±0.13 a
	RP₃	15.48±1.80 c	0.28±0.02 ab	0.54±0.11 b	0.86±0.24 c

检测年份 Detection year	根系修剪处理 Root prunning treatments	每株侧根数 Lateral root number per plant	根系总长度 Total root length/cm	表面积 Surface area/cm²	平均长度 Average length/cm
2018	RP₀	148±18 b	254.37±49.02 b	21.35±3.08 b	1.14±0.19 a
	RP₁	164±21 b	235.59±38.41 b	20.06±2.08 b	1.21±0.0.9 a
	RP₂	250±19 a	355.54±28.34 a	33.86±3.53 a	1.31±0.08 a
	RP₃	181±23 b	202.63±16.26 b	16.83±1.39 b	1.06±0.13 a
2019	RP₀	135±11 b	255.20±6.27 b	20.67±0.69 b	1.15±0.12 b
	RP₁	155±10 b	230.02±11.16 b	20.05±0.41 b	1.18±0.10 b
	RP₂	237±23 a	368.73±14.86 a	35.43±2.16 a	1.32±0.06 a
	RP₃	179±16 ab	215.65±17.05 b	18.75±2.08 b	1.03±0.08 b

Effects of root pruning on growth and expression of related genes in Poncirus trifoliata

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检测年份 Detection year	根系修剪处理 Root pruning treatments	大量元素含量 Macro-element content/%			中量元素含量 Secondary element content/%		微量元素含量 Trace-element content/(mg·kg^-1)
检测年份 Detection year	根系修剪处理 Root pruning treatments	N	P	K	Ca	Mg	B	Fe	Na	Zn
2018	RP₀	1.72 ±0.51 c	0.50 ±0.07 b	1.46 ±0.27 c	3.96 ±0.37 c	1.25 ±0.04 c	25.37 ±3.96 c	82.68 ±3.92 c	73.51 ±21.81 c	8.39 ±4.17 c
	RP₁	2.16 ±0.26 ab	0.59 ±0.07 b	2.08 ±0.87 b	4.60 ±1.00 bc	1.42 ±0.18 bc	44.61 ±4.34 a	89.06 ±4.99 bc	90.38 ±13.75 b	10.19 ±7.00 bc
	RP₂	1.99 ±0.05 b	0.78 ±0.11 a	2.67 ±1.08 ab	7.17 ±0.34 a	1.61 ±0.21 b	41.57 ±1.92 ab	125.17 ±5.18 a	108.25 ±7.76 a	19.85 ±3.64 ab
	RP₃	2.26 ±0.15 a	0.72 ±0.04 ab	3.68 ±0.28 a	5.95 ±1.24 ab	1.94 ±0.05 a	26.85 ±1.96 bc	114.41 ±6.43 ab	85.91 ±4.99 bc	25.19 ±2.02 a
2019	RP₀	1.91 ±0.02 b	0.60 ±0.04 c	2.73 ±1.22 b	5.49 ±1.24 c	1.52 ±0.21 b	28.11 ±6.44 b	80.50 ±29.82 b	53.24 ±8.70 b	11.88 ±6.67 c
	RP₁	2.23 ±0.02 ab	0.82 ±0.05 a	3.07 ±0.19 ab	6.93 ±1.08 ab	1.68 ±0.37 ab	34.36 ±1.11 ab	87.34 ±15.41 b	64.35 ±15.65 b	14.85 ±8.46 bc
	RP₂	2.16 ±0.19 b	0.76 ±0.02 ab	3.11 ±0.67 a	7.09 ±0.44 a	1.70 ±0.10 ab	40.38 ±2.93 a	147.03 ±24.8 a	90.37 ±13.75 a	27.01 ±3.75 a
	RP₃	2.32 ±0.03 a	0.74 ±0.05 b	3.17 ±0.71 a	6.46 ±1.10 bc	1.80 ±0.18 a	28.72 ±3.66 b	98.57 ±12.81 b	79.38 ±3.51 b	25.18 ±2.02 ab

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