Identification and expression analysis of polyamine oxidase (PAO) gene family in apple

doi:10.3969/j.issn.1004-1524.2020.02.10

›› 2020, Vol. 32 ›› Issue (2): 262-273.DOI: 10.3969/j.issn.1004-1524.2020.02.10

• Horticultural Science • Previous Articles Next Articles

Identification and expression analysis of polyamine oxidase (PAO) gene family in apple

QIN Ling¹, ZHANG Xin¹, RONG Chunxiao¹, MO Chuanyuan¹, FAN Lu¹, YAN Jie¹, MENG Ying², ZHANG Manrang^1,*

1. College of Horticulture, Northwest A&F University, Yangling 712100, China;
2. Forestry Bureau of Luonan County, Luonan 726100, China

Received:2019-07-11 Online:2020-02-25 Published:2020-03-13

Abstract

Abstract: From the genomic database of Golden Delicious, eight family genes encoding polyamine oxidase (PAO) (named as MdPAO1-8) were identified in apples using the BLAST website for physicochemical properties, phylogenetic, protein structure, analysis of gene structure and promoter elements. The results showed that the molecular weight of MdPAO protein ranged from 54.053 to 64.813 ku, and the isoelectric point ranged from 5.16 to 6.02. According to the phylogenetic tree analysis, MdPAO is divided into three subfamilies (Ⅰ,Ⅲ, Ⅳ), and members of the same subfamily share similar protein structure, gene structure and protein conserved motif distribution. The expression of 8 MdPAO genes in different tissues and organs was significantly different using the GEO database. The expression of 8 MdPAO genes in different tissues was determined by real-time quantitative PCR using Nagafu No.2. The results showed that MdPAO3, MdPAO4, MdPAO5/6 and MdPAO8 were highly espressed in flowers. MdPAO1/2 and MdPAO7 had higher expression levels in stems and fruits. Exogenous spermidine treatment showed that the transcriptional level of MdPAO significantly increased except MdPAO3, indicating that MdPAO plays an important role in the PA metabolic pathway.

Key words: apple, polyamine oxidase (PAO) gene family, bioinformatics, gene expression

CLC Number:

S661.1

QIN Ling, ZHANG Xin, RONG Chunxiao, MO Chuanyuan, FAN Lu, YAN Jie, MENG Ying, ZHANG Manrang. Identification and expression analysis of polyamine oxidase (PAO) gene family in apple[J]. , 2020, 32(2): 262-273.

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Identification and expression analysis of polyamine oxidase (PAO) gene family in apple

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