Cloning and bioinformatics analysis of GH gene and its flanking region in Odontobutis potamophila

doi:10.3969/j.issn.1004-1524.2019.09.09

›› 2019, Vol. 31 ›› Issue (9): 1461-1470.DOI: 10.3969/j.issn.1004-1524.2019.09.09

• Animal Science • Previous Articles Next Articles

Cloning and bioinformatics analysis of GH gene and its flanking region in Odontobutis potamophila

LIU Jialin^1,2, LIU Shili^2,3,4, JIANG Wenping², CHENG Shun², CHI Meili², ZHENG Jianbo², JIA Yongyi², ZHAO Jinliang^3,4, YIN Shaowu^1,*, GU Zhimin²

1. School of Life Science, Nanjing Normal University, Nanjing 210000, China;
2. Key Laboratory of Healthy Freshwater Aquaculture of Ministry of Agriculture and Rural Development / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China;
3. Key Laboratory of Freshwater Aquaculture Germplasm Resources, Ministry of Agriculture and Rural Development, Shanghai Ocean University, Shanghai 201306, China;
4. Shanghai Aquaculture Engineering and Technology Research Center, Shanghai Ocean University, Shanghai 201306, China

Received:2019-03-07 Online:2019-09-25 Published:2019-10-11

Abstract

Abstract: The full-length sequence of the Odontobutis potamophila GH gene was successfully cloned by using long segment PCR, genome walking method and T-A cloning technology, which was submitted to GenBank to obtain the login number MH717101. The full-length of the GH gene of Odontobutis potamophila is 5 120 bp, the length of the 5'-end and 3'-end lateral sequence is 920 bp and 682 bp. It is predicted that the upstream region contains MF3, GAGA factor, hnf-3alpha, C/EBP, R2, GATA-1 and other transcriptional regulatory elements, respectively. The transcription unit is 3 518 bp long and contains 4 introns and 5 exons. 4 introns are 75, 333, 2 070 and 121 bp; 5 exons are 150, 131, 117, 138 and 373 bp, respectively. The third intron is 2 070 bp, the length was more than 4 times of the culter third intron. The open reading frame area (ORF) is 594 bp, encoding a protein polypeptide composed of 197 amino acid residues. Comparison of amino acid sequence homology between Odontobutis potamophila and Perciformes, Scorpaeniformes, Cypriniformes, Siluriformes with 13 fishes showed that the homology was the highest to Leucopsarion petersii, at 83.8%, and the homology to Ctenopharyngodon idella was the lowest, at 49.1%, the Odontobutis potamophila to show highly nonconservative at 180-197 amino acid sequence and the amino acid arrangement was completely different with the listed fish. The results of this study laid a molecular foundation for further study on expression, function and transcriptional regulation of GH gene in Odontobutis potamophila.

Key words: Odontobutis potamophila, growth hormone, clone, sequence analysis, bioinformatics

CLC Number:

S917

LIU Jialin, LIU Shili, JIANG Wenping, CHENG Shun, CHI Meili, ZHENG Jianbo, JIA Yongyi, ZHAO Jinliang, YIN Shaowu, GU Zhimin. Cloning and bioinformatics analysis of GH gene and its flanking region in Odontobutis potamophila[J]. , 2019, 31(9): 1461-1470.

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Cloning and bioinformatics analysis of GH gene and its flanking region in Odontobutis potamophila

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