Bioinformatics analysis of StUOXs gene family in potato

doi:10.3969/j.issn.1004-1524.2020.09.01

›› 2020, Vol. 32 ›› Issue (9): 1523-1532.DOI: 10.3969/j.issn.1004-1524.2020.09.01

• Crop Science • Previous Articles Next Articles

Bioinformatics analysis of StUOXs gene family in potato

LIANG Liqin^a,b, YANG Rui^a, GAO Gang^a,*

a. College of Life Sciences, Shanxi Normal University, Linfen 041004, China;
b. College of Arts and Sciences, Shanxi Normal University, Linfen 041004, China

Received:2020-03-14 Online:2020-09-25 Published:2020-10-10

Abstract

Abstract: Potato(Solanum tuberosum) is the third largest food crop in the world, and its yield and quality are adversely affected by biotic and abiotic stresses. Ubiquinone oxidase(UOX), as a mitochondrial terminal oxidase encoded by nuclear genes, plays an important role in the regulation of plant growth and stress response. In order to understand the response of StUOXs in potato disease resistance, five StUOXs genes were identified in potato genome by bioinformatics methods, and their physical and chemical properties, chromosome location, tertiary structure, evolutionary relationship, conserved domain, promoter element and expression profile, especially the expression patterns induced by Ralstonia solanacearum were analyzed. The results showed that the number of amino acids encoded by potato StUOXs genes was between 279 and 366, the molecular weight was distributed between 31 and 42 ku, and the subcellular locations were located in mitochondria. Phylogenetic analysis showed that potato StUOXs members were closely related to tomato and pepper UOXs members, but slightly farther than Arabidopsis thaliana and tobacco. FPKM data analysis showed that StUOXs were mainly induced by salt, hormone and biological stress in leaves, which corresponded to the cis-acting elements such as hormone response in its promoter. The qRT-PCR test after inoculation with Ralstonia solanacearum showed that StUOX4 was involved in the early response to the bacterial wilt pathogens.

Key words: potato, StUOXs, sequences characteristics, bioinformatics analysis

CLC Number:

S532

LIANG Liqin, YANG Rui, GAO Gang. Bioinformatics analysis of StUOXs gene family in potato[J]. , 2020, 32(9): 1523-1532.

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Bioinformatics analysis of StUOXs gene family in potato

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