Pathogen identification of bacterial fruit blotch of melon on sweet potato in Zhejiang Province

doi:10.3969/j.issn.1004-1524.2019.07.11

›› 2019, Vol. 31 ›› Issue (7): 1112-1118.DOI: 10.3969/j.issn.1004-1524.2019.07.11

• Plant Protection • Previous Articles Next Articles

Pathogen identification of bacterial fruit blotch of melon on sweet potato in Zhejiang Province

HONG Xianxian¹, WU Xiuqin¹, LUO Jinyan², WANG Yanli³, SUN Guocang³, AN Qianli¹, QIU Wen^1,*, LI Bin¹

1. Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture and Rural Affairs, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China;
2. Shanghai Agriculture Technology Extension & Service Center, Shanghai 201103, China;
3. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

Received:2018-12-13 Online:2019-07-25 Published:2019-08-07

Abstract

Abstract: Bacterial stem and root rot of sweet potato is an important quarantine disease. In the current study, four isolates of suspected diseased sweet potato induced a hypersensitive response on tobacco plants. These isolates were then identified into Acidovorax citrulli (formerly Acidovorax avenae subsp. citrulli) based on morphology, Gram's stain reaction, 16S rRNA sequence and pathogenicity analysis. Results here showed that the sequence homology of the novel isolates was as high as 99.00%-100.00% compared with A. citrulli, the bacterial pathogen of bacterial fruit blotch of cucurbits, which was the key quarantine pathogen of China. The current report indicated the importance of disease management for efficient controlling of bacterial fruit blotch of watermelon in Zhejiang Province.

Key words: sweet potato, Acidovorax citrulli, bacterial fruit blotch of watermelon, identification, quarantine

CLC Number:

S435.311

HONG Xianxian, WU Xiuqin, LUO Jinyan, WANG Yanli, SUN Guocang, AN Qianli, QIU Wen, LI Bin. Pathogen identification of bacterial fruit blotch of melon on sweet potato in Zhejiang Province[J]. , 2019, 31(7): 1112-1118.

References

[1] CRALL J M, SCHENCK N C .Bacterial fruit rot of watermelon in Florida[J].Plant Disease Reporter,1969,53: 74-75.
[2] 蔡学清, 黄月英, 杨建珍, 等. 福建省西瓜细菌性果斑病的病原鉴定[J]. 福建农林大学学报(自然科学版), 2005, 34(4): 434-437.
CAI X Q, HUANG Y Y, YANG J Z, et al.Pathogen identification of bacterial fruit blotch of watermelon in Fujian[J]. Journal of Fujian Agriculture and Forestry University(Natural Science Edition), 2005, 34(4): 434-437.(in Chinese with English abstract)
[3] 张敏荣, 余继华, 卢璐, 等. 台州新发现甘薯茎腐病[J]. 植物检疫, 2016, 30(3): 84-86.
ZHANG M R, YU J H, LU L, et al.Stem rot of sweet potato newly discovered in Taizhou[J]. Plant Quarantine, 2016, 30(3): 84-86.(in Chinese with English abstract)
[4] 徐玉恒, 张永涛, 陈香艳, 等. 春甘薯西瓜套作高效种植模式[J]. 山东农业科学, 2005, 37(3): 40.
XU Y H, ZHANG Y T, CHEN X Y, et al.High efficiency planting model of spring sweet potato watermelon intercropping[J]. Shandong Agricultural Sciences, 2005, 37(3): 40.(in Chinese)
[5] 张则林. 西瓜套种甘薯高效栽培模式[J]. 河北农业科技, 2007(3): 6.
ZHANG Z L.High-efficiency cultivation mode about interplanting of watermelon and sweet potato[J]. Modern Rural Science and Technology, 2007(3):6. (in Chinese)
[6] 明华. 甘薯西瓜套种技术[J]. 农村实用技术, 2010(5): 47.
MING H.Interplanting technique of sweet potato and watermelon[J]. Rural Practical Technology, 2010(5):47. (in Chinese)
[7] 赵廷昌, 孙福在, 王兵万, 等. 哈密瓜细菌性果斑病病原菌鉴定[J]. 植物病理学报, 2001, 31(4): 357-364.
ZHAO T C, SUN F Z, WANG B W, et al.Pathogen identification of Hami melon bacterial fruit blotch[J]. Acta Phytopathologica Sinica, 2001, 31(4): 357-364.(in Chinese with English abstract)
[8] 方中达. 植病研究方法[M]. 3版. 北京: 中国农业出版社, 1998.
[9] WALCOTT R R, GITAITIS R D.Detection of acidovorax avenae subsp. citrulli in watermelon seed using immunomagnetic separation and the polymerase chain reaction[J]. Plant Disease, 2000, 84(4): 470-474.
[10] 徐福寿, 王笑, 谢关林, 等. 免疫捕捉PCR法检测西瓜种子带细菌性果斑病菌[J]. 果树学报, 2008, 25(2): 215-218.
XU F S, WANG X, XIE G L, et al.Detection of Acidovorax avenae subsp. citrulli from seeds of watermelon by immuno-capture PCR[J]. Journal of Fruit Science, 2008, 25(2): 215-218.(in Chinese with English abstract)
[11] 冯建军, 许勇, 李健强, 等. 免疫凝聚试纸条和TaqMan探针实时荧光PCR检测西瓜细菌性果斑病菌比较研究[J]. 植物病理学报, 2006, 36(2): 102-108.
FENG J J, XU Y, LI J Q, et al.Comparison of immunoStrip and real-time fluorescent PCR (TaqMan) for detection of Acidovorax avenae subsp. citrulli, the causal agent of bacterial fruit blotch of watermelon[J]. Acta Phytopathologica Sinica, 2006, 36(2): 102-108.(in Chinese with English abstract)
[12] 陈新垲. 西瓜细菌性果斑病菌的PCR检测[D]. 福州: 福建农林大学, 2010.
CHEN X K.Detection the pathogen of bacterial fruit blotch of watermelon by polymerase chain reaction[D]. Fuzhou: Fujian Agriculture and Forestry University, 2010.(in Chinese with English abstract)
[13] 沈肖玲, 林钗, 钱俊婷, 等. 甘薯茎腐病症状及其病原鉴定[J]. 植物病理学报, 2018, 48(1): 25-34.
SHEN X L, LIN C, QIAN J T, et al.Characterization of stem and root rot symptoms of sweet potato and the causal pathogen of the disease[J]. Acta Phytopathologica Sinica, 2018, 48(1): 25-34.(in Chinese with English abstract)
[14] 邢东光. 西瓜细菌性果斑病发生与防治[J]. 北方园艺, 2002(3): 69.
XING D G.The occurance and prevention of bacterical fruit blotch of watermelon[J]. Northern Horticulture, 2002(3):69. (in Chinesse )
[15] 孟昭军, 郭卜, 吴连凯, 等. 西瓜、厚皮甜瓜果斑病防治进展[J]. 蔬菜, 2004(3): 22-23.
MENG Z J, GUO B, WU L K, et al.The progresses in prevention and treatment of spot disease of watermelon and thick-skinned melon[J]. Vegetables, 2004(3):22-23. (in Chinese)

Pathogen identification of bacterial fruit blotch of melon on sweet potato in Zhejiang Province

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YANG Mei, HU Xiaolan, SHEN Tao, TAN Kang, LIU Dailing, QIU Hongbo. Construction of single fragment substitution lines of maize 8th chromosome and sreening of resistant maize germplasm to gray leaf spot [J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 383-389.
[2]	XIE Ziyu, WANG Ke’er, ZHAO Wenliang, WEN Zuhui, CHEN Linrun, XU Lishan. Nutritional components and bioactivities of sweet potatoes with different flesh colors [J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 183-192.
[3]	LIU Lei1, WANG Xun, LUO Yi, LIANG Jiyuan, YAN Peiqi, LI Xinyi, LIU Wei, LIU Chenkai, LI Jiajia. Isolation, culture and identification of pigeon intestine epithelium cells [J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 239-247.
[4]	XIN Xiaoting, LIU Daqun, ZHANG Chengcheng, WU Min, CHEN Denggao, ZHANG Jianming. Screening, identification and application of high efficient nitrite degrading functional strains in Chinese characteristic fermented vegetables [J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 335-345.
[5]	CHEN Wenqiang, WANG Xiaofu, CHEN Xiaoyun, PENG Cheng, XU Junfeng, CAI Jian. Preliminary study on identification of Dendrobium officinale from Zhejiang based on ITS2 and SNP technology [J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 69-76.
[6]	XU Xuefen, NI Chunhui, LI Huixia, LI Huanyu, LI Wenhao, CHEN Yuan, HU Fangdi. Pathogen identification and indoor toxicity tests on root rot of Codonopsis pilosula [J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 96-103.
[7]	LIAO Chaomei, YOU Minfang, TAN Guanghui, LI Jiezhang, ZHANG Yiyu, QIN Yuanyu, LIU Ruoyu. Effect of variation of PRKCB gene intron 14 in Sansui ducks on eggshell quality [J]. , 2020, 32(9): 1574-1580.
[8]	ZHANG Xiaoyan, HE Jing, HOU Caixia, ZHANG Shuheng. Screening and identification of antagonistic strains of wolfberry root rot [J]. , 2020, 32(5): 858-865.
[9]	GUO Xuesong, TIAN Libo, SHANG Sang, ZOU Kaixi, CHEN Hongrong, LI Wanyu, YUE Xiaoqi. Isolation, identification and characterization of antagonistic actinomycetes A10 and A17 against Botryodiplodia theobromae [J]. , 2020, 32(3): 460-468.
[10]	WU Weicheng, DAI Jianbo, CAO Yan, XIA Qile, CHEN Jianbing, MENG Xianghe. Effects of physical modification on content, polysaccharide composition and structure of dietary fiber in sweet potato peels [J]. , 2020, 32(3): 490-498.
[11]	LI Xuqing, YAN Jianli, RUAN Songlin. Identification and biological characteristics of anthracnose pathogen on Tetrastigma hemsleyanum [J]. , 2020, 32(11): 2009-2019.
[12]	YUAN Xianyu, YANG Longbin, HE Zanzan, MAO Tianjiao, HE Changsheng, ZHAN Songhe, SUN Pei, WEI Jianzhong, LI Yu. Isolation and identification of pseudorabies virus and molecular characterization of its main virulence genes in Anhui [J]. , 2020, 32(1): 43-56.
[13]	YI Keke, YIN Wenqi, ZHOU Yuancheng, JIANG Jinzhen, ZHANG Baiyu, LI Zhongyin, YAN Qigui. Isolation and identification of 4 strains of porcine pseudorabies virus and analysis of main virulence genes [J]. , 2019, 31(9): 1429-1436.
[14]	YANG Yibin, AI Xiaohui, SONG Yi, DONG Jing, XU Ning, JIANG Lan. Preliminary study on hemolytic ascites disease of Pelteobagrus fulvidraco [J]. , 2019, 31(8): 1239-1248.
[15]	YU Mingtao, ZHANG Kefeng. Identification of soil hydraulic parameters based on HYDRUS-2D software and simulation of soil water movement under indirect subsurface drip irrigation [J]. , 2019, 31(3): 458-468.