Pathogen identification and indoor toxicity tests on root rot of Codonopsis pilosula

doi:10.3969/j.issn.1004-1524.2021.01.12

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (1): 96-103.DOI: 10.3969/j.issn.1004-1524.2021.01.12

• Plant Protection • Previous Articles Next Articles

Pathogen identification and indoor toxicity tests on root rot of Codonopsis pilosula

XU Xuefen¹(), NI Chunhui¹, LI Huixia¹^,^*(), LI Huanyu¹, LI Wenhao¹, CHEN Yuan², HU Fangdi³

1. College of Plant Protection, Gansu Agricultural University, Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou 730070, China
2. College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China
3. College of Pharmacy, Lanzhou University, Lanzhou 730030, China

Received:2020-03-21 Online:2021-01-25 Published:2021-01-25
Contact: LI Huixia

Abstract

Abstract:

In order to identify the pathogenic fungi causing the root rot of Codonopsis pilosula in Weiyuan County of Gansu Province and screen the best fungicides to control the disease, the pathogen causing the root rot of Codonopsis pilosula was isolated and purified by tissue isolation and single spore isolation. The pathogen was identified by pathogenicity determination, colony morphological characteristics, and clustering analysis of coding transcription extension factor region (TEF-1α). The toxicity was determined by growth rate method. The results of pathogen identification showed that Fusarium solani and Fusarium acuminatum were the pathogens causing root rot of Codonopsis pilosula in Weiyuan County of Gansu Province. The results of virulence test showed that all the six fungicides had inhibitory effect on them. Among them, 25% myclobutanil EC and 50% carbendazim WP had strong control effect (EC₅₀<0.05 g·L ^-1), which could be used as the main fungicides to control the root rot of Codonopsis pilosula.

Key words: Codonopsis pilosula, root rot, Fusarium solani, Fusarium acuminatum, identification, fungicide

CLC Number:

S435.67

XU Xuefen, NI Chunhui, LI Huixia, LI Huanyu, LI Wenhao, CHEN Yuan, HU Fangdi. Pathogen identification and indoor toxicity tests on root rot of Codonopsis pilosula[J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 96-103.

Figures/Tables 8

References 23

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药剂名称 Reagent name	体积分数 Volume fraction/%	剂型 Dosage form	生产商 Manufacturer
腈菌唑 Myclobutanil	25	乳油 EC	山东曹达化工有限公司Shandong Caoda Chemical Co., Ltd
多菌灵 Carbendazim	50	可湿性粉剂WP	山东鑫星农药有限公司Shandong Xinxing Pesticide Co., Ltd
嘧菌酯 Azoxystrobin	25	悬浮剂SC	盐城利民农化有限公司Yancheng Limin Agrochemical Co., Ltd
代森锰锌 Mancozeb	80	可湿性粉剂WP	济南农化工有限公司Jinan Agricultural Chemical Co., Ltd
中生菌素 Mesosporin	3	可湿性粉剂WP	福建凯立生物制品有限公司Fujian Kaili Biological Products Co., Ltd
福美双 Thiram	50	可湿性粉剂WP	济南农化工有限公司Jinan Agricultural Chemical Co., Ltd

药剂名称 Reagent name	体积分数 Volume fraction/%	剂型 Dosage form	生产商 Manufacturer
腈菌唑 Myclobutanil	25	乳油 EC	山东曹达化工有限公司Shandong Caoda Chemical Co., Ltd
多菌灵 Carbendazim	50	可湿性粉剂WP	山东鑫星农药有限公司Shandong Xinxing Pesticide Co., Ltd
嘧菌酯 Azoxystrobin	25	悬浮剂SC	盐城利民农化有限公司Yancheng Limin Agrochemical Co., Ltd
代森锰锌 Mancozeb	80	可湿性粉剂WP	济南农化工有限公司Jinan Agricultural Chemical Co., Ltd
中生菌素 Mesosporin	3	可湿性粉剂WP	福建凯立生物制品有限公司Fujian Kaili Biological Products Co., Ltd
福美双 Thiram	50	可湿性粉剂WP	济南农化工有限公司Jinan Agricultural Chemical Co., Ltd

药剂名称Reagent name	质量浓度Mass concentration/(g·L^-1)
腈菌唑 Myclobutanil	0.100	0.125	0.167	0.250	0.500
多菌灵 Carbendazim	0.100	0.125	0.167	0.250	0.500
嘧菌酯 Azoxystrobin	0.333	0.417	0.556	0.833	1.667
代森锰锌 Mancozeb	0.400	0.500	0.667	1.000	2.000
中生菌素 Mesosporin	0.333	0.417	0.556	0.833	1.667
福美双 Thiram	0.400	0.500	0.667	1.000	2.000

药剂名称Reagent name	质量浓度Mass concentration/(g·L^-1)
腈菌唑 Myclobutanil	0.100	0.125	0.167	0.250	0.500
多菌灵 Carbendazim	0.100	0.125	0.167	0.250	0.500
嘧菌酯 Azoxystrobin	0.333	0.417	0.556	0.833	1.667
代森锰锌 Mancozeb	0.400	0.500	0.667	1.000	2.000
中生菌素 Mesosporin	0.333	0.417	0.556	0.833	1.667
福美双 Thiram	0.400	0.500	0.667	1.000	2.000

试剂名称 Reagent name	回归方程 Regression equation	相关系数 R²	EC₅₀/ (g·L^-1)
腈菌唑 Myclobutanil	y=0.515x+6.177	0.683	0.005 2
多菌灵 Carbendazim	y=3.743x+10.152	0.812	0.042 0
嘧菌酯 Azoxystrobin	y=0.287x+4.932	0.911	1.725 4
代森锰锌 Mancozeb	y=1.732x+5.589	0.853	0.457 0
中生菌素 Mesosporin	y=1.260x+4.858	0.881	1.296 3
福美双 Thiram	y=3.667x+7.008	0.721	0.283 4

Pathogen identification and indoor toxicity tests on root rot of Codonopsis pilosula

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药剂名称 Reagent name	回归方程 Regression equation	相关系数 R²	EC₅₀/ (g·L^-1)
腈菌唑 Myclobutanil	y=0.695x+6.071	0.937	0.028 7
多菌灵 Carbendazim	y=0.888x+7.226	0.981	0.003 1
嘧菌酯 Azoxystrobin	y=0.656x+6.213	0.898	0.014 2
代森锰锌 Mancozeb	y=4.832x+6.918	0.926	0.400 9
中生菌素 Mesosporin	y=0.759x+5.975	0.958	0.051 9
福美双 Thiram	y=0.421x+5.866	0.911	0.008 8

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