Screening and identification of antagonistic strains of wolfberry root rot

doi:10.3969/j.issn.1004-1524.2020.05.14

›› 2020, Vol. 32 ›› Issue (5): 858-865.DOI: 10.3969/j.issn.1004-1524.2020.05.14

• Plant Protection • Previous Articles Next Articles

Screening and identification of antagonistic strains of wolfberry root rot

ZHANG Xiaoyan, HE Jing^*, HOU Caixia, ZHANG Shuheng

College of Forestry, Gansu Agricultural University, Lanzhou 730070, China

Received:2019-11-08 Online:2020-05-25 Published:2020-05-29

Abstract

Abstract: Wolfberry diseases and insect pests are important factors affecting the development of wolfberry industry, among which the root rot of wolfberry is a common disease. In this study, healthy wolfberry rhizosphere soil samples were selected from seriously diseased wolfberry plantations and strains were isolated by conventional dilution method. Fusarium solani, the main pathogen of wolfberry root rot, was selected as the tested strain by plate confrontation method, and the antagonistic strain was identified by morphology and molecular biology. The results showed that 101 strains of bacteria, 52 strains of actinomycetes, 30 strains of mycetes and 27 strains of saccharomycetes were isolated from rhizosphere soil samples of healthy wolfberry. Two antagonistic strains, HYM-19 and HYC-7, were screened and identified as Sinorhizobium sp. and Metarhizium sp., respectively. The results provided a theoretical basis for the effective use of soil microorganisms to manage root rot of wolfberry.

Key words: wolfberry, root rot, antagonistic bacteria, screening, identification

CLC Number:

S436.639

ZHANG Xiaoyan, HE Jing, HOU Caixia, ZHANG Shuheng. Screening and identification of antagonistic strains of wolfberry root rot[J]. , 2020, 32(5): 858-865.

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Screening and identification of antagonistic strains of wolfberry root rot

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