Screening and identification of antagonistic strains of wolfberry root rot

doi:10.3969/j.issn.1004-1524.2020.05.14

›› 2020, Vol. 32 ›› Issue (5): 858-865.DOI: 10.3969/j.issn.1004-1524.2020.05.14

• Plant Protection • Previous Articles Next Articles

Screening and identification of antagonistic strains of wolfberry root rot

ZHANG Xiaoyan, HE Jing^*, HOU Caixia, ZHANG Shuheng

College of Forestry, Gansu Agricultural University, Lanzhou 730070, China

Received:2019-11-08 Online:2020-05-25 Published:2020-05-29

Abstract

Abstract: Wolfberry diseases and insect pests are important factors affecting the development of wolfberry industry, among which the root rot of wolfberry is a common disease. In this study, healthy wolfberry rhizosphere soil samples were selected from seriously diseased wolfberry plantations and strains were isolated by conventional dilution method. Fusarium solani, the main pathogen of wolfberry root rot, was selected as the tested strain by plate confrontation method, and the antagonistic strain was identified by morphology and molecular biology. The results showed that 101 strains of bacteria, 52 strains of actinomycetes, 30 strains of mycetes and 27 strains of saccharomycetes were isolated from rhizosphere soil samples of healthy wolfberry. Two antagonistic strains, HYM-19 and HYC-7, were screened and identified as Sinorhizobium sp. and Metarhizium sp., respectively. The results provided a theoretical basis for the effective use of soil microorganisms to manage root rot of wolfberry.

Key words: wolfberry, root rot, antagonistic bacteria, screening, identification

CLC Number:

S436.639

ZHANG Xiaoyan, HE Jing, HOU Caixia, ZHANG Shuheng. Screening and identification of antagonistic strains of wolfberry root rot[J]. , 2020, 32(5): 858-865.

References

[1] FUKUDA T, YOKOYAMA J, OHASHI H.Phylogeny and biogeography of the genus Lycium(Solanaceae): inferences from chloroplast DNA sequences[J]. Molecular Phylogenetics and Evolution, 2001, 19(2): 246-258.
[2] AMAGASE H, FARNSWORTH N R.A review of botanical characteristics, phytochemistry, clinical relevance in efficacy and safety of Lycium barbarum fruit (Goji)[J]. Food Research International, 2011, 44(7): 1702-1717.
[3] 郑国琦, 胡正海. 宁夏枸杞的生物学和化学成分的研究进展[J]. 中草药, 2008, 39(5): 796-800.
ZHENG G Q, HU Z H.Advances in research on biological and chemical constituents of Ningxia wolfberry[J]. Chinese Traditional and Herbal Drugs, 2008, 39(5): 796-800.(in Chinese)
[4] LUO Q, CAI Y Z, YAN J, et al.Hypoglycemic and hypolipidemic effects and antioxidant activity of fruit extracts from Lycium barbarum[J]. Life Sciences, 2004, 76(2): 137-149.
[5] AMAGASE H, SUN B X, BOREK C.Lycium barbarum(goji) juice improves in vivo antioxidant biomarkers in serum of healthy adults[J]. Nutrition Research, 2009, 29(1): 19-25.
[6] 申培增, 关参政. 几种杀螨剂对枸杞瘿螨的防治效果比较[J]. 经济林研究, 2013, 31(3): 140-142.
SHEN P Z, GUAN C Z.Comparison of effect of four kinds of acaricides against Aceria macrodonis[J]. Nonwood Forest Research, 2013, 31(3): 140-142.(in Chinese with English abstract)
[7] 朱会文. 甘肃省景电灌区枸杞根腐病的发生与防治[J]. 现代农业科技, 2018(5): 121.
ZHU H W.Occurrence and control of root rot of Lycium barbarum in Jingdian Irrigation Area of Gansu Province[J]. Modern Agricultural Science and Technology, 2018(5): 121.(in Chinese)
[8] 鲁占魁, 王国珍, 张丽荣, 等. 枸杞根腐病的发生及防治研究[J]. 植物保护学报, 1994, 21(3): 249-254.
LU Z K, WANG G Z, ZHANG L R, et al.Occurence and control of wolfberry root rot[J]. Journal of Plant Protection, 1994, 21(3): 249-254.(in Chinese with English abstract)
[9] 王国珍, 鲁占魁. 宁夏枸杞根腐病病原的研究[J]. 微生物学通报, 1994, 21(6): 330-332.
WANG G Z, LU Z K.Study on the pathogen of root rot of wolfberry in Ningxia[J]. Microbiology, 1994, 21(6): 330-332.(in Chinese)
[10] 李捷. 甘肃省枸杞根腐病病原及生理生化抗病机理研究[D]. 兰州: 甘肃农业大学, 2015.
LI J.The root rot pathogens of Lycium bararum in Gansu Province and physiological biochemical mechanism of resistance[D]. Lanzhou: Gansu Agricultural University, 2015.(in Chinese with English abstract)
[11] 刘淑娟. 景泰县草窝滩镇枸杞根腐病的发生及防治[J]. 防护林科技, 2009(3): 116-117.
LIU S J.Occurrence and prevention of root rot of Chinese wolfberry in Caowutan Town, Jingtai County[J]. Protection Forest Science and Technology, 2009(3): 116-117.(in Chinese)
[12] 杜红霞. 枸杞病虫害综合防治[J]. 新疆农业科技, 1999(4): 16.
DU H X.Comprehensive control of wolfberry pests and diseases[J]. Xinjiang Agricultural Science and Technology, 1999(4): 16.(in Chinese)
[13] 陈伶俐, 马洪福, 李亚娟, 等. 10种杀菌剂对枸杞根腐病菌的室内毒力测定[J]. 青海大学学报(自然科学版), 2015, 33(5): 10-13.
CHEN L L, MA H F, LI Y J, et al.Toxicity measurement of 10 fungicides on Fusarium causing wolf berry rot root[J]. Journal of Qinghai University(Natural Science Edition), 2015, 33(5): 10-13.(in Chinese with English abstract)
[14] 陈伟, 何静, 多甜甜, 等. 几种杀菌剂对枸杞根腐病菌的室内毒力测定[J]. 甘肃农业大学学报, 2017, 52(1): 109-113.
CHEN W, HE J, DUO T T, et al.Toxicity test on several fungicides against Chinese wolfberry root rot in vitro[J]. Journal of Gansu Agricultural University, 2017, 52(1): 109-113.(in Chinese with English abstract)
[15] DRUZHININA I S, KUBICEK C P, KOMON-ZELAZOWSKA M, et al.The Trichoderma harzianum demon: complex speciation history resulting in coexistence of hypothetical biological species, recent agamospecies and numerous relict lineages[J]. BMC Evolutionary Biology, 2010, 10(1): 94.
[16] TAYLOR J W, GEISER D M, BURT A, et al.The evolutionary biology and population genetics underlying fungal strain typing[J]. Clinical Microbiology Reviews, 1999, 12(1): 126-146.
[17] 薛应钰, 范万泽, 张树武, 等. 苹果树腐烂病菌拮抗放线菌的筛选、鉴定及防效[J]. 应用生态学报, 2016, 27(10): 3379-3386.
XUE Y Y, FAN W Z, ZHANG S W, et al.Screening, identification and biocontrol effect of antagonistic actinomycetes against the pathogen of Cytospora sp.for apple tree[J]. Chinese Journal of Applied Ecology, 2016, 27(10): 3379-3386.(in Chinese with English abstract)
[18] 李兴龙, 李彦忠. 土传病害生物防治研究进展[J]. 草业学报, 2015, 24(3): 204-212.
LI X L, LI Y Z.Research advances in biological control of soil-borne disease[J]. Acta Prataculturae Sinica, 2015, 24(3): 204-212.(in Chinese with English abstract)
[19] 杜士杰. 微生物源农药的应用现状及发展研究[J]. 黑龙江科技信息, 2016(25): 155.
DU S J.Application and development of microbial pesticide[J]. Heilongjiang Science and Technology Information, 2016(25): 155.(in Chinese)
[20] 张振华, 林江, 王文雅, 等. 火龙果溃疡病原菌拮抗菌株的筛选与生物防治效果初探[J]. 河南农业科学, 2019,48(8): 88-94.
ZHANG Z H, LIN J, WANG W Y, et al.Screening of antagonistic strains of pitaya ulcer pathogen and primary study on biocontrol effects[J]. Journal of Henan Agricultural Sciences, 2019,48(8): 88-94.
[21] 马锦锦. 枸杞根际促生细菌筛选、培养基优化及基因组测序[D]. 泰安: 山东农业大学, 2017.
MA J J.Screening, medium optimization and genome sequencing of PGRP from the rhizosphere of Lycium barbarum L.[D]. Tai'an: Shandong Agricultural University, 2017.(in Chinese with English abstract)
[22] 毛仪楠, 邓百万. 白芨病菌拮抗菌株的筛选及鉴定[J]. 江苏农业科学, 2019, 47(3): 103-106.
MAO Y N, DENG B W.Screening and identification of antagonistic strains of Bletilla striata pathogenic bacteria[J]. Jiangsu Agricultural Sciences, 2019, 47(3): 103-106.(in Chinese)
[23] 郝士海. 现代细菌学培养基和生化试验手册[M]. 北京:中国科学技术出版社, 1992.
[24] 陈伶俐. 柴达木地区枸杞根腐病病原菌生物学特性及药剂防治研究[D]. 西宁: 青海大学, 2015.
CHEN L L.Biological characteristics of pathogens causing wolf berry root rot and fungicides controlling experiment[D]. Xining: Qinghai University, 2015.(in Chinese with English abstract)
[25] 陈君, 张建文, 方琴, 等. 宁夏中宁枸杞炭疽病的发生与防治[J]. 基层中药杂志, 2002, 16(3): 41-42.
CHEN J, ZHANG J W, FANG Q, et al.Occurrence and control of anthracnose in Zhongning, Ningxia[J]. Primary Journal of Chinese Materia Medica, 2002, 16(3): 41-42.(in Chinese)
[26] 梅新兰, 赵青云, 谭石勇, 等. 辣椒疫病拮抗菌株筛选、鉴定及其防效[J]. 应用生态学报, 2010, 21(10): 2652-2658.
MEI X L, ZHAO Q Y, TAN S Y, et al.Screening, identification, and biocontrol effect of antagonistic bacteria against Phytophthora capsici[J]. Chinese Journal of Applied Ecology, 2010, 21(10): 2652-2658.(in Chinese with English abstract)
[27] ABANDA-NKPWATT D, KRIMM U, SCHREIBER L, et al.Dual antagonism of aldehydes and epiphytic bacteria from strawberry leaf surfaces against the pathogenic fungus Botrytis cinerea in vitro[J]. Biocontrol, 2006, 51(3): 279-291.
[28] PALANIYANDI S A, YANG S H, ZHANG L X, et al.Effects of actinobacteria on plant disease suppression and growth promotion[J]. Applied Microbiology and Biotechnology, 2013, 97(22): 9621-9636.
[29] 邱德文. 我国植物病害生物防治的现状及发展策略[J]. 植物保护, 2010, 36(4): 15-18.
QIU D W.Current status and development strategy for biological control of plant diseases in China[J]. Plant Protection, 2010, 36(4): 15-18.(in Chinese with English abstract)
[30] 刘明稀, 易自力, 赵运林, 等. 草坪病害生物防治研究进展[J]. 草业学报, 2004, 13(6): 1-7.
LIU M X, YI Z L, ZHAO Y L, et al.Research advances of biological control on turfgrass diseases[J]. Acta Pratacultural Science, 2004, 13(6): 1-7.(in Chinese with English abstract)
[31] ALABOUVETTE C, OLIVAIN C, STEINBERG C.Biological control of plant diseases: the European situation[J]. European Journal of Plant Pathology, 2006, 114(3): 329-341.
[32] 孙冬梅, 杨谦, 宋金柱. 黄绿木霉菌对大豆根腐病镰刀菌的拮抗作用[J]. 中国油料作物学报, 2005, 27(3): 58-61.
SUN D M, YANG Q, SONG J Z.Antagonism of Trichoderma aureoviride against Fusarium spp which causes soybean root rot[J]. Chinese Journal of Oil Crop Scieves, 2005, 27(3): 58-61.(in Chinese with English abstract)
[33] 勾长龙, 王雨琼, 孙朋, 等. 人参根腐病拮抗菌的筛选、鉴定及其抑菌活性[J]. 食品科学, 2015, 36(19): 143-147.
GOU C L, WANG Y Q, SUN P, et al.Screening and identification of an antagonistic strain against Fusarium solani and analysis of its antipathogenic activity[J]. Food Science, 2015, 36(19): 143-147.(in Chinese with English abstract)
[34] 黄铭慧. 大豆尖镰孢根腐病拮抗菌X2生防菌剂的研制与应用[D]. 哈尔滨: 东北农业大学, 2017.
HUANG M H.Research and application on antagonistic bacteria (X2) powder for suppressing soybean root rot induced by Fusarium oxysporum[D]. Harbin: Northeast Agricultural University, 2017.(in Chinese with English abstract)
[35] 齐永霞, 陈方新, 李增智. 金龟子绿僵菌对棉花枯萎病菌的拮抗作用研究[J]. 棉花学报, 2010, 22(6): 591-596.
QI Y X, CHEN F X, LI Z Z.Inhibitory mechanisms of Metarhizium anisopliae against the pathogens of Fusarium wilt of cotton[J]. Cotton Science, 2010, 22(6): 591-596.(in Chinese with English abstract)
[36] 齐永霞, 陈方新, 李增智. 绿僵菌对小麦纹枯病菌的抑制作用研究[J]. 激光生物学报, 2011, 20(6): 758-764.
QI Y X, CHEN F X, LI Z Z.Inhititory mechanisms of Metarhizium anisopliae against Rhizoctonia cerealis[J]. Acta Laser Biology Sinica, 2011, 20(6): 758-764.(in Chinese with English abstract)
[37] 杨帆, 刘春来, 王爽, 等. 一株平沙绿僵菌的鉴定及生防应用潜力评价[J]. 植物保护, 2018, 44(5): 199-205.
YANG F, LIU C L, WANG S, et al.Identification of Metarhizium pingshaense and assessing potentiality of bio-control application[J]. Plant Protection, 2018, 44(5): 199-205.(in Chinese with English abstract)
[38] 李云玲, 侯沁文, 刘瑞祥, 等. 草木樨中华根瘤菌CHW₁₀B溶磷特性及其对南方红豆杉的促生作用[J]. 林业科学研究, 2017, 30(5): 751-758.
LI Y L, HOU Q W, LIU R X, et al.Phosphate-solubilizing characteristics and growth-promoting effect of Sinorhizobium meliloti CHW10B on Taxus chinensis var. mairei[J]. Forest Research, 2017, 30(5): 751-758.(in Chinese with English abstract)
[39] 夏铁骑. 植物根际促生菌及其应用研究[J]. 济源职业技术学院学报, 2008, 7(3): 7-11.
XIA T Q.Research and application of plant growth-promoting rhizobacteria[J]. Journal of Jiyuan Vocational and Technical College, 2008, 7(3): 7-11.(in Chinese with English abstract)
[40] 徐琳, 石建锋. 分子生物学技术在根瘤菌遗传多样性研究中的应用[J]. 河西学院学报, 2011, 27(2): 78-84.
XU L, SHI J F.Application of molecular biology technology on rhizobial genetic diversity[J]. Journal of Hexi University, 2011, 27(2): 78-84.(in Chinese with English abstract)

Screening and identification of antagonistic strains of wolfberry root rot

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