Screening and identification of an antagonistic fungus strain against root rot disease in tea plants and its biological characteristics

doi:10.3969/j.issn.1004-1524.20240565

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (7): 1469-1480.DOI: 10.3969/j.issn.1004-1524.20240565

• Plant Protection • Previous Articles Next Articles

Screening and identification of an antagonistic fungus strain against root rot disease in tea plants and its biological characteristics

DENG Yulian¹^,²(), TAN Lin³, NIU Li¹^,², LU Qiancheng¹^,², ZHOU Linghong⁴, LI Guihua¹^,², HU Qiulong¹^,²^,^*()

1. Key Lab of Tea Science, Ministry of Education, Hunan Agricultural University, Changsha 410128, China
2. College of Horticulture, Hunan Agricultural University, Changsha 410128, China
3. College of Plant Protection, Hunan Agricultural University, Changsha 410128, China
4. Chenzhou Institute of Agricultural Science, Chenzhou 423001, Hunan, China

Received:2024-06-27 Online:2025-07-25 Published:2025-08-20

Abstract

Abstract:

In the present study, a fungal isolate with antagonistic effect on tea root rot pathogen was screened from the rhizosphere soil of tea plants infected by tea root rot pathogen through plate confrontation method. The isolated strain was identified by the morphological and molecular biology methods, and its biological characteristics were studied, as well as its antimicrobial spectrum, antimicrobial ability of metabolites and extracellular hydrolase activities. The results showed that the obtained strain FX2 was finally identified as Penicillium citrinum, which exhibited an inhibition rate of 72.98% against the pathogen Fusarium cugenangense of tea root rot. The strain FX2 was relatively insensitive to the carbon-nitrogen ratio. The suitable carbon source of this strain was lactose, the suitable nitrogen source was peptone or yeast extract, the optimal sulfate was MgSO₄ or K₂SO₄. The tolerance range of this strain to NaCl was 0-140 g·L^-1, the optimal pH value was 6, and the maximum withstand temperature was 60 ℃. The ultraviolet light irradiation exhibited certain inhibitory effect on the growth of this strain. The inhibition rate of this strain against other 8 plant pathogens (including Alternaria alternata, Fusarium graminearum, Exserohilum turcicum, Diaporthe citri, Fusarium oxysporum, Colletotrichum circinans, Colletotrichum camelliae, Pseudopestalotiopsis theae) were higher than 50%, indicating broad antibacterial spectrum. The inhibition rate of 5%, 10%, 15% and 20% sterile fermentation filtrate against root rot pathogen was 35.12%, 45.98%, 52.80% and 59.46%, respectively, and the inhibition rate of non-volatile metabolites and volatile metabolites against root rot pathogen was 71.73% and 22.14%, respectively. Besides, this strain also had the ability to secrete proteases, amylases, chitinases and cellulases. In conclusion, the isolated strain FX2 was a promising biocontrol resource for tea root rot.

Key words: tea tree, root rot, antagonistic fungi, inhibition rate, biological properties

CLC Number:

S432

DENG Yulian, TAN Lin, NIU Li, LU Qiancheng, ZHOU Linghong, LI Guihua, HU Qiulong. Screening and identification of an antagonistic fungus strain against root rot disease in tea plants and its biological characteristics[J]. Acta Agriculturae Zhejiangensis, 2025, 37(7): 1469-1480.

Figures/Tables 10

Fig.1 Inhibitory effect of strain FX2 against Fusarium cugenangense A, Control (CK); B, Treatment with strain FX2.

Fig.2 Effect of strain FX2 on hypha growth of Fusarium cugenangense A, Control (CK); B, Treatment with strain FX2.

Fig.3 Colony and morphological characteristics of strain FX2 A and B, C and D, E and F show the front and back of colony morphologies of strain FX2 cultured on OA、MEA、PDA medium at 28 ℃ for 3 days, respectively. G and H show the morphology of mycelium and spores of strain FX2 cultured on PDA medium, respectively.

Fig.4 Phylogenetic tree of strain FX2 based on ITS sequence Numbers at branch nodes present bootstrap value. The scale bar 0.01 represents sequence variance. “T” indicates standard strain.

Fig.5 Biological characteristics of strain FX2 CK represents the control treatment. Bars marked without the same letters indicate significant difference at P<0.05. The same as below.

Fig.6 Inhibition effect of strain FX2 against 8 plant pathogens

Table 1 Inhibition effect of sterile fermentation filtrate of strain FX2

无菌发酵滤液的体积分数 Volume fraction of sterile fermentation filtrate/%	菌落直径 Colony diameter/mm	抑制率 Inhibition rate/%
0	78.00±0.41 a	—
5	50.60±0.43 b	35.12±0.56 d
10	42.13±0.81 c	45.98±1.04 c
15	36.80±1.16 d	52.80±1.49 b
20	31.61±0.45 e	59.46±0.57 a

Fig.7 Inhibition effect of volatile metabolites of strain FX2 Left and right show control and treatment, respecitvley. The same as in Fig. 8.

Fig.8 Inhibition effect of non-volatile metabolites of strain FX2

Fig.9 Abilities of strain FX2 to produce extracellular hydrolase A, Protease; B, Amylase; C, Chitinase; D, Cellulase。

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Screening and identification of an antagonistic fungus strain against root rot disease in tea plants and its biological characteristics

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