Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (7): 1459-1468.DOI: 10.3969/j.issn.1004-1524.20240589
• Horticultural Science • Previous Articles Next Articles
TAN Yali1(), GAO Mengxiang1, LI Xiaojie2,*(
), ZHOU Yingjie2, XIONG Jian2, ZENG Ziqi2, LI Xiao2, YANG Hua3
Received:
2024-07-04
Online:
2025-07-25
Published:
2025-08-20
CLC Number:
TAN Yali, GAO Mengxiang, LI Xiaojie, ZHOU Yingjie, XIONG Jian, ZENG Ziqi, LI Xiao, YANG Hua. Quality of mainly cultivated walnuts in Hubei Province of China at different harvest periods[J]. Acta Agriculturae Zhejiangensis, 2025, 37(7): 1459-1468.
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URL: http://www.zjnyxb.cn/EN/10.3969/j.issn.1004-1524.20240589
Fig.1 Transverse and longitudinal diameters of walnuts at different harvest periods The absence of identical capital letters on the same column indicates significant differences between different varieties during the same harvesting period(P<0.05). The absence of identical lowercase letters on the same colored column indicates significant differences between different harvest periods for the same variety(P<0.05). Fig. 2-Fig. 5 are the same.
氨基酸种类 Types of amino acid | 不同采收期含量Contents at different harvest periods | |||||||
---|---|---|---|---|---|---|---|---|
秭林一号Zilin No. 1 | 清香Qingxiang | |||||||
Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅰ | Ⅱ | Ⅲ | Ⅳ | |
天冬氨酸Aspartic acid | 34.8±0.6 a | 25.1±0.4 b | 23.1±0.3 c | 25.0±0.1 b | 24.1±0.8 a | 10.6±0.3 c | 15.1±1.0 b | 15.8±0.7 b |
苏氨酸*Threonine | 8.7±0.2 a | 7.6±0.8 ab | 6.7±0.5 b | 6.8±0.7 b | 8.0±0.8 a | 4.1±0.4 c | 5.8±1.1 b | 5.9±1.1 b |
丝氨酸Serine | 12.4±0.2 a | 11.4±1.6 a | 10.8±0.1 a | 11.1±1.1 a | 10.6±2.9 a | 6.0±0.4 b | 9.2±1.7 ab | 9.4±2.0 ab |
谷氨酸Glutamate | 67.1±0.9 a | 47.0±0.6 b | 43.6±0.3 c | 38.7±0.3 d | 40.0±0.4 a | 20.0±0.4 c | 30.4±0.3 b | 31.2±1.4 b |
甘氨酸Glycine | 12.6±0.2 a | 10.2±0.3 b | 10.3±0.5 b | 9.7±1.4 b | 9.0±2.3 a | 5.6±0.3 b | 8.9±1.0 a | 8.7±2.1 ab |
丙氨酸Alanine | 10.2±0.9 a | 10.0±0.9 a | 10.2±0.5 a | 9.6±0.9 a | 8.9±2.2 a | 5.8±0.8 a | 8.6±0.6 a | 8.4±2.2 a |
胱氨酸Cystine | 1.8±0.1 a | 1.9±0.4 a | 1.0±0.2 b | 0.7±0.3 b | 2.2±0.3 a | 1.2±0.4 b | 0.8±0.2 b | 1.1±0.8 b |
缬氨酸*Valine | 10.7±0.8a | 10.7±0.8 a | 10.2±0.3 a | 10.7±0.8 a | 10.5±2.2 a | 5.7±0.2 b | 8.6±0.6 a | 8.4±0.7 a |
异亮氨酸*Isoleucine | 8.9±0.3 a | 8.2±0.6 ab | 8.5±0.4 ab | 7.6±0.7 b | 9.3±1.7 a | 4.4±0.2 c | 6.9±0.5 b | 6.4±1.4 b |
亮氨酸*Leucine | 29.0±0.2 a | 17.1±0.6 b | 15.5±0.3 c | 15.3±0.2 c | 20.9±0.5 a | 9.6±0.2 d | 12.8±0.2 b | 10.4±0.5 c |
酪氨酸Tyrosine | 12.3±0.2 a | 2.5±1.6 d | 8.1±0.1 b | 5.3±0.4 c | 9.4±0.4 a | 5.6±0.7 b | 7.6±0.3 ab | 4.7±0.7 b |
苯丙氨酸*Phenylalanine | 10.1±0.4 a | 10.2±0.7 a | 10.2±0.3 a | 10.2±0.7 a | 10.5±1.7 b | 5.8±0.7 d | 14.0±0.9 a | 8.4±0.8 c |
赖氨酸*Lysine | 6.5±0.2 a | 6.7±0.4 a | 5.4±0.2 b | 5.9±0.4 b | 8.0±0.3 a | 3.6±0.3 d | 6.7±0.3 b | 5.3±1.0 c |
组氨酸Histidine | 6.4±0.2 a | 5.4±0.5 b | 4.3±0.2 c | 4.9±0.6 bc | 5.7±1.1 a | 2.7±0.2 c | 5.0±0.4 ab | 4.3±0.8 b |
精氨酸Arginine | 50.2±0.4 a | 29.6±0.6 c | 28.3±0.5 d | 38.7±0.4 b | 30.1±0.4 a | 16.2±0.3 d | 24.2±0.3 c | 27.1±0.9 b |
脯氨酸Proline | 10.1±0.2 b | 8.5±0.4 c | 6.8±0.1 d | 14.2±0.8 a | 7.7±0.4 b | 4.4±0.3 c | 6.8±0.7 b | 11.4±2.5 a |
Table 1 Composition and contents of amino acids of walnuts at different harvest periods g·kg-1
氨基酸种类 Types of amino acid | 不同采收期含量Contents at different harvest periods | |||||||
---|---|---|---|---|---|---|---|---|
秭林一号Zilin No. 1 | 清香Qingxiang | |||||||
Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅰ | Ⅱ | Ⅲ | Ⅳ | |
天冬氨酸Aspartic acid | 34.8±0.6 a | 25.1±0.4 b | 23.1±0.3 c | 25.0±0.1 b | 24.1±0.8 a | 10.6±0.3 c | 15.1±1.0 b | 15.8±0.7 b |
苏氨酸*Threonine | 8.7±0.2 a | 7.6±0.8 ab | 6.7±0.5 b | 6.8±0.7 b | 8.0±0.8 a | 4.1±0.4 c | 5.8±1.1 b | 5.9±1.1 b |
丝氨酸Serine | 12.4±0.2 a | 11.4±1.6 a | 10.8±0.1 a | 11.1±1.1 a | 10.6±2.9 a | 6.0±0.4 b | 9.2±1.7 ab | 9.4±2.0 ab |
谷氨酸Glutamate | 67.1±0.9 a | 47.0±0.6 b | 43.6±0.3 c | 38.7±0.3 d | 40.0±0.4 a | 20.0±0.4 c | 30.4±0.3 b | 31.2±1.4 b |
甘氨酸Glycine | 12.6±0.2 a | 10.2±0.3 b | 10.3±0.5 b | 9.7±1.4 b | 9.0±2.3 a | 5.6±0.3 b | 8.9±1.0 a | 8.7±2.1 ab |
丙氨酸Alanine | 10.2±0.9 a | 10.0±0.9 a | 10.2±0.5 a | 9.6±0.9 a | 8.9±2.2 a | 5.8±0.8 a | 8.6±0.6 a | 8.4±2.2 a |
胱氨酸Cystine | 1.8±0.1 a | 1.9±0.4 a | 1.0±0.2 b | 0.7±0.3 b | 2.2±0.3 a | 1.2±0.4 b | 0.8±0.2 b | 1.1±0.8 b |
缬氨酸*Valine | 10.7±0.8a | 10.7±0.8 a | 10.2±0.3 a | 10.7±0.8 a | 10.5±2.2 a | 5.7±0.2 b | 8.6±0.6 a | 8.4±0.7 a |
异亮氨酸*Isoleucine | 8.9±0.3 a | 8.2±0.6 ab | 8.5±0.4 ab | 7.6±0.7 b | 9.3±1.7 a | 4.4±0.2 c | 6.9±0.5 b | 6.4±1.4 b |
亮氨酸*Leucine | 29.0±0.2 a | 17.1±0.6 b | 15.5±0.3 c | 15.3±0.2 c | 20.9±0.5 a | 9.6±0.2 d | 12.8±0.2 b | 10.4±0.5 c |
酪氨酸Tyrosine | 12.3±0.2 a | 2.5±1.6 d | 8.1±0.1 b | 5.3±0.4 c | 9.4±0.4 a | 5.6±0.7 b | 7.6±0.3 ab | 4.7±0.7 b |
苯丙氨酸*Phenylalanine | 10.1±0.4 a | 10.2±0.7 a | 10.2±0.3 a | 10.2±0.7 a | 10.5±1.7 b | 5.8±0.7 d | 14.0±0.9 a | 8.4±0.8 c |
赖氨酸*Lysine | 6.5±0.2 a | 6.7±0.4 a | 5.4±0.2 b | 5.9±0.4 b | 8.0±0.3 a | 3.6±0.3 d | 6.7±0.3 b | 5.3±1.0 c |
组氨酸Histidine | 6.4±0.2 a | 5.4±0.5 b | 4.3±0.2 c | 4.9±0.6 bc | 5.7±1.1 a | 2.7±0.2 c | 5.0±0.4 ab | 4.3±0.8 b |
精氨酸Arginine | 50.2±0.4 a | 29.6±0.6 c | 28.3±0.5 d | 38.7±0.4 b | 30.1±0.4 a | 16.2±0.3 d | 24.2±0.3 c | 27.1±0.9 b |
脯氨酸Proline | 10.1±0.2 b | 8.5±0.4 c | 6.8±0.1 d | 14.2±0.8 a | 7.7±0.4 b | 4.4±0.3 c | 6.8±0.7 b | 11.4±2.5 a |
指标 | TD | LD | KY | GFD | MC | FAT | PRO | TAA | Glu | Arg | Asp | Ca |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Index | ||||||||||||
LD | 0.935** | |||||||||||
KY | 0.557** | 0.629** | ||||||||||
GFD | 0.597** | 0.651** | 0.832** | |||||||||
MC | -0.394 | -0.472* | -0.796** | -0.795** | ||||||||
FAT | 0.420* | 0.442* | 0.560** | 0.790** | -0.844** | |||||||
PRO | -0.009 | -0.099 | -0.550** | -0.526** | 0.857** | -0.721** | ||||||
TAA | 0.145 | 0.130 | -0.276 | -0.208 | 0.648** | -0.554** | 0.840** | |||||
Glu | 0.145 | 0.100 | -0.273 | -0.326 | 0.700** | -0.680** | 0.887** | 0.956** | ||||
Arg | 0.263 | 0.225 | -0.141 | -0.032 | 0.546** | -0.458* | 0.808** | 0.937** | 0.893** | |||
Asp | 0.193 | 0.149 | -0.323 | -0.271 | 0.679** | 0.557** | 0.892** | 0.970** | 0.964** | 0.925** | ||
Ca | -0.265 | -0.340 | -0.659** | -0.650** | 0.964** | -0.838** | 0.911** | 0.768** | 0.814** | 0.714** | 0.785** | |
Zn | 0.269 | 0.220 | -0.337 | -0.257 | 0.649** | -0.496* | 0.906** | 0.916** | 0.917** | 0.897** | 0.968** | 0.755** |
Table 2 Correlation between each quality indicator of walnuts
指标 | TD | LD | KY | GFD | MC | FAT | PRO | TAA | Glu | Arg | Asp | Ca |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Index | ||||||||||||
LD | 0.935** | |||||||||||
KY | 0.557** | 0.629** | ||||||||||
GFD | 0.597** | 0.651** | 0.832** | |||||||||
MC | -0.394 | -0.472* | -0.796** | -0.795** | ||||||||
FAT | 0.420* | 0.442* | 0.560** | 0.790** | -0.844** | |||||||
PRO | -0.009 | -0.099 | -0.550** | -0.526** | 0.857** | -0.721** | ||||||
TAA | 0.145 | 0.130 | -0.276 | -0.208 | 0.648** | -0.554** | 0.840** | |||||
Glu | 0.145 | 0.100 | -0.273 | -0.326 | 0.700** | -0.680** | 0.887** | 0.956** | ||||
Arg | 0.263 | 0.225 | -0.141 | -0.032 | 0.546** | -0.458* | 0.808** | 0.937** | 0.893** | |||
Asp | 0.193 | 0.149 | -0.323 | -0.271 | 0.679** | 0.557** | 0.892** | 0.970** | 0.964** | 0.925** | ||
Ca | -0.265 | -0.340 | -0.659** | -0.650** | 0.964** | -0.838** | 0.911** | 0.768** | 0.814** | 0.714** | 0.785** | |
Zn | 0.269 | 0.220 | -0.337 | -0.257 | 0.649** | -0.496* | 0.906** | 0.916** | 0.917** | 0.897** | 0.968** | 0.755** |
变量 Variable | 主成分1 PC1 | 主成分2 PC2 |
---|---|---|
蛋白质含量Protein content | 0.98 | -0.08 |
谷氨酸含量Glutamate content | 0.96 | 0.15 |
天冬氨酸含量Aspartic acid content | 0.96 | 0.26 |
总氨基酸含量Total amino acid content | 0.95 | 0.28 |
锌含量Zinc content | 0.93 | 0.29 |
钙含量Calcium content | 0.92 | -0.34 |
精氨酸含量Arginine content | 0.89 | 0.37 |
含水率Moisture content | 0.84 | -0.50 |
脂肪含量Fat content | -0.75 | 0.58 |
特征值Eigenvalue | 7.487 | 1.096 |
方差贡献率Variance contribution rate/% | 83.184 | 12.172 |
累计贡献率Cumulative contribution rate/% | 83.184 | 95.356 |
Table 3 Variance eigenvalues, contribution rates, and load matrix values of principal components
变量 Variable | 主成分1 PC1 | 主成分2 PC2 |
---|---|---|
蛋白质含量Protein content | 0.98 | -0.08 |
谷氨酸含量Glutamate content | 0.96 | 0.15 |
天冬氨酸含量Aspartic acid content | 0.96 | 0.26 |
总氨基酸含量Total amino acid content | 0.95 | 0.28 |
锌含量Zinc content | 0.93 | 0.29 |
钙含量Calcium content | 0.92 | -0.34 |
精氨酸含量Arginine content | 0.89 | 0.37 |
含水率Moisture content | 0.84 | -0.50 |
脂肪含量Fat content | -0.75 | 0.58 |
特征值Eigenvalue | 7.487 | 1.096 |
方差贡献率Variance contribution rate/% | 83.184 | 12.172 |
累计贡献率Cumulative contribution rate/% | 83.184 | 95.356 |
品种 Variety | 采收期 Harvest period | X1 含水率 Moisture content | X2 脂肪含量 Fat content | X3 蛋白质含量 Protein content | X4 总氨基酸含量 Total amino acid content | X5 谷氨酸含量 Glutamate content | X6 精氨酸含量 Arginine content | X7 天冬氨酸含量 Aspartic acid content | X8 钙含量 Calcium content | X9 锌含量 Zinc content |
---|---|---|---|---|---|---|---|---|---|---|
秭林一号 | Ⅰ | -0.33 | 0.39 | -0.06 | 0.10 | 0.28 | -0.22 | 0.19 | -0.24 | 0.43 |
Zilin No. 1 | Ⅱ | 0.23 | -0.58 | 0.34 | 0.27 | 0.52 | -0.10 | 0.44 | -0.05 | 0.26 |
Ⅲ | -0.82 | 1.22 | 0.04 | 0.32 | -0.07 | 0.80 | 0.43 | -0.59 | 0.66 | |
Ⅳ | 1.78 | -2.00 | 1.97 | 1.81 | 1.96 | 1.94 | 1.73 | 2.11 | 1.67 | |
清香 | Ⅰ | -1.11 | -0.09 | -0.52 | 0.32 | 0.01 | -0.04 | 0.31 | 0.81 | 0.21 |
Qingxiang | Ⅱ | -0.10 | -0.05 | -0.69 | 1.68 | -1.41 | -1.42 | 1.46 | -0.40 | -1.19 |
Ⅲ | -0.87 | 0.10 | 1.12 | -0.52 | -0.67 | -0.63 | 0.87 | 0.83 | -1.09 | |
Ⅳ | 0.99 | 1.00 | 1.01 | 0.60 | 0.61 | 0.34 | 0.77 | -0.81 | -0.95 |
Table 4 Standardized values of each index
品种 Variety | 采收期 Harvest period | X1 含水率 Moisture content | X2 脂肪含量 Fat content | X3 蛋白质含量 Protein content | X4 总氨基酸含量 Total amino acid content | X5 谷氨酸含量 Glutamate content | X6 精氨酸含量 Arginine content | X7 天冬氨酸含量 Aspartic acid content | X8 钙含量 Calcium content | X9 锌含量 Zinc content |
---|---|---|---|---|---|---|---|---|---|---|
秭林一号 | Ⅰ | -0.33 | 0.39 | -0.06 | 0.10 | 0.28 | -0.22 | 0.19 | -0.24 | 0.43 |
Zilin No. 1 | Ⅱ | 0.23 | -0.58 | 0.34 | 0.27 | 0.52 | -0.10 | 0.44 | -0.05 | 0.26 |
Ⅲ | -0.82 | 1.22 | 0.04 | 0.32 | -0.07 | 0.80 | 0.43 | -0.59 | 0.66 | |
Ⅳ | 1.78 | -2.00 | 1.97 | 1.81 | 1.96 | 1.94 | 1.73 | 2.11 | 1.67 | |
清香 | Ⅰ | -1.11 | -0.09 | -0.52 | 0.32 | 0.01 | -0.04 | 0.31 | 0.81 | 0.21 |
Qingxiang | Ⅱ | -0.10 | -0.05 | -0.69 | 1.68 | -1.41 | -1.42 | 1.46 | -0.40 | -1.19 |
Ⅲ | -0.87 | 0.10 | 1.12 | -0.52 | -0.67 | -0.63 | 0.87 | 0.83 | -1.09 | |
Ⅳ | 0.99 | 1.00 | 1.01 | 0.60 | 0.61 | 0.34 | 0.77 | -0.81 | -0.95 |
品种 Variety | 采收期 Harvest period | F1 | F2 | F | 排名 Ranking |
---|---|---|---|---|---|
秭林一号 | Ⅰ | -0.08 | 0.66 | 0.02 | 4 |
Zilin No. 1 | Ⅱ | 0.29 | 0.56 | 0.31 | 3 |
Ⅲ | 0.28 | 0.84 | 0.34 | 2 | |
Ⅳ | 3.32 | 0.99 | 2.89 | 1 | |
清香 | Ⅰ | -0.22 | -0.11 | -0.20 | 4 |
Qingxiang | Ⅱ | 0.01 | 0.40 | 0.05 | 3 |
Ⅲ | 0.33 | -0.45 | 0.22 | 2 | |
Ⅳ | 1.86 | 0.66 | 1.63 | 1 |
Table 5 Comprehensive score and ranking of Zilin No.1 and Qingxiang Walnut at different harvest periods
品种 Variety | 采收期 Harvest period | F1 | F2 | F | 排名 Ranking |
---|---|---|---|---|---|
秭林一号 | Ⅰ | -0.08 | 0.66 | 0.02 | 4 |
Zilin No. 1 | Ⅱ | 0.29 | 0.56 | 0.31 | 3 |
Ⅲ | 0.28 | 0.84 | 0.34 | 2 | |
Ⅳ | 3.32 | 0.99 | 2.89 | 1 | |
清香 | Ⅰ | -0.22 | -0.11 | -0.20 | 4 |
Qingxiang | Ⅱ | 0.01 | 0.40 | 0.05 | 3 |
Ⅲ | 0.33 | -0.45 | 0.22 | 2 | |
Ⅳ | 1.86 | 0.66 | 1.63 | 1 |
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