Effects of stocking modes on growth traits and muscle nutritional composition of Macrobrachium nipponense

doi:10.3969/j.issn.1004-1524.20221043

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (2): 254-263.DOI: 10.3969/j.issn.1004-1524.20221043

• Animal Science • Previous Articles Next Articles

Effects of stocking modes on growth traits and muscle nutritional composition of Macrobrachium nipponense

TANG Jinyu¹^,²(), QIN Baoli², YE Jianyong², DAI Yangxin³

1. Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. Suqian Institute of Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Suqian 223800, China
3. Institute of Fisheries Research, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China

Received:2022-07-15 Online:2024-02-25 Published:2024-03-05

Abstract

Abstract:

In the present study, 3 types of stocking modes were introduced as treatments, namely, T1, coculture of oriental river prawn (Macrobrachium nipponense) with Chinese mitten crab (Eriocheir sinensis) and some economic fish species; T2, coculture of M.nipponense with dark sleeper Odontobutis obscurus; T3, coculture of M.nipponense with bighead carp Aristichthys nobilis. Their influences on the growth traits and muscle nutritional composition of M. nipponense was evaluated. Results showed that the total length, carapace length, body length and wet weight of M. nipponense were significantly (P<0.05) different among treatments. The varations in contents of crude protein, crude lipid, ash and moisture in the muscle of M. nipponense were 16.54%-17.97%, 1.86%-2.25%, 0.95%-1.12% and 78.69%-80.56%, respectively, of which the ash content was significantly different. A total of 17 amino acids, including 7 essential amino acids were identified, and the essential amino acid index (EAAI) was 70.88-73.13. The contents of alanine were significantly different among treatments. A total of 16 fatty acids were found, and the fatty acid composition and the contents of C18:2 n-6 and polyunsaturated fatty acid were significantly affected by stocking modes. Nine mineral elements, including Na, K, Ca, Mg, Fe, Cu, Zn, Mn and Cr were monitored and the contents of Na, Mn, Fe, Zn were significantly different among treatments. These findings indicated that the alteration of food availability and environmental factors caused by stocking modes could affect the growth traits and muscle nutritional values. Thus, it is necessary to maintain these nutritive substances after changing the aquaculture modes. By comparison, it was concluded that coculture with Chinese mitten crab and some economic fish species could improve the delicious taste, nutritional value and healthcare values of the cultured prawn, which could be served as a promising mode in the further freshwater aquaculture practice.

Key words: Macrobrachium nipponense, stocking mode, growth traits, muscle nutritional composition, nutritional value

CLC Number:

S966.12

TANG Jinyu, QIN Baoli, YE Jianyong, DAI Yangxin. Effects of stocking modes on growth traits and muscle nutritional composition of Macrobrachium nipponense[J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 254-263.

Figures/Tables 6

References 35

[1]	2023中国渔业统计年鉴[M]. 北京: 中国农业出版社, 2023.
[2]	唐金玉. 鱼蚌综合养殖池塘养殖模式优化的研究[D]. 杭州: 浙江大学, 2016.
	TANG J Y. The studies for optimizing the mode of integrated culture of freshwater pearl mussel and fishes in ponds[D]. Hangzhou: Zhejiang University, 2016. (in Chinese with English abstract)
[3]	王岩. 海水池塘养殖模式优化: 概念、原理与方法[J]. 水产学报, 2004, 28(5): 568-572.
	WANG Y. Optimization of culture model in seawater pond: concepts, principles and methods[J]. Journal of Fisheries of China, 2004, 28(5): 568-572. (in Chinese with English abstract)
[4]	TANG J Y, DAI Y X, WANG Y, et al. Improvement of fish and pearl yields and nutrient utilization efficiency through fish-mussel integration and feed supplementation[J]. Aquaculture, 2015, 448: 321-326.
[5]	唐金玉, 王岩, 任岗, 等. 摄食配合饲料的鱼密度和EM菌对蚌鱼综合养殖系统浮游植物的影响[J]. 水产学报, 2019, 43(6): 1438-1448.
	TANG J Y, WANG Y, REN G, et al. Effects of stocking density of the fishes fed formulated feed and EM product on phytoplankton community in a mussel-fish integrated system[J]. Journal of Fisheries of China, 2019, 43(6): 1438-1448. (in Chinese with English abstract)
[6]	戴杨鑫, 王岩, 唐金玉, 等. 不同混养鱼类和投喂方式对鱼蚌综合养殖水体化学特征的影响[J]. 中国水产科学, 2013, 20(2): 351-360.
	DAI Y X, WANG Y, TANG J Y, et al. Effects of co-cultured fish and feed supplement on water chemistry characteristics of freshwater pearl mussel farming[J]. Journal of Fishery Sciences of China, 2013, 20(2): 351-360. (in Chinese with English abstract)
[7]	HOU D W, HUANG Z J, ZENG S Z, et al. Environmental factors shape water microbial community structure and function in shrimp cultural enclosure ecosystems[J]. Frontiers in Microbiology, 2017, 8: 2359.
[8]	王志铮, 赵晶, 杨磊, 等. 三种养殖模式下日本鳗鲡(Anguilla japonica)养成品血清生化指标和脏器消化酶、抗氧化酶活力的差异[J]. 海洋与湖沼, 2013, 44(2): 403-408.
	WANG Z Z, ZHAO J, YANG L, et al. Variations of serum biochemical indices, viscera digestive enzymes activity and antioxidant enzyme activity of three cultured population of Anguilla japonica[J]. Oceanologia et Limnologia Sinica, 2013, 44(2): 403-408. (in Chinese with English abstract)
[9]	马玲巧, 亓成龙, 曹静静, 等. 水库网箱和池塘养殖斑点叉尾鮰肌肉营养成分和品质的比较分析[J]. 水产学报, 2014, 38(4): 531-536.
	MA L Q, QI C L, CAO J J, et al. Comparative study on muscle texture profile and nutritional value of channel catfish (Ictalurus punctatus) reared in ponds and reservoir cages[J]. Journal of Fisheries of China, 2014, 38(4): 531-536. (in Chinese with English abstract)
[10]	高露姣, 黄艳青, 夏连军, 等. 不同养殖模式下红鳍东方鲀的品质比较[J]. 水产学报, 2011, 35(11): 1668-1676.
	GAO L J, HUANG Y Q, XIA L J, et al. Comparison of flesh quality of farmed Fugu, Takifugu rubripes from different culture models[J]. Journal of Fisheries of China, 2011, 35(11): 1668-1676. (in Chinese with English abstract)
[11]	万金娟, 陈友明, 邵俊杰, 等. 盱眙地区不同养殖模式下克氏原螯虾肌肉品质的比较分析[J]. 动物营养学报, 2020, 32(2): 965-972.
	WAN J J, CHEN Y M, SHAO J J, et al. Comparative analysis on muscle quality of red swamp crayfish(Procambarus clarkia)cultured under different aquaculture modes in Xuyi region[J]. Chinese Journal of Animal Nutrition, 2020, 32(2): 965-972. (in Chinese with English abstract)
[12]	程辉辉, 谢从新, 李大鹏, 等. 种青养鱼模式下的草鱼肌肉营养成分和品质特性[J]. 水产学报, 2016, 40(7): 1050-1059.
	CHENG H H, XIE C X, LI D P, et al. The study of muscular nutritional components and fish quality of grass carp(Ctenopharyngodon idella) in ecological model of cultivating grass carp with grass[J]. Journal of Fisheries of China, 2016, 40(7): 1050-1059. (in Chinese with English abstract)
[13]	HUANG Y J, ZHANG N N, FAN W J, et al. Soybean and cottonseed meals are good candidates for fishmeal replacement in the diet of juvenile Macrobrachium nipponense[J]. Aquaculture International, 2018, 26(1): 309-324.
[14]	孙盛明, 戈贤平, 苏艳莉, 等. 池塘投喂膨化饲料主养模式对鳙形体指标、肌肉营养成分和品质特性的影响[J]. 动物营养学报, 2020, 32(5): 2379-2386.
	SUN S M, GE X P, SU Y L, et al. Effects of pond mono-culture pattern with expanded feed on morphological indices, muscular nutrient composition and quality characteristic of bighead carp(aristichthys nobilis)[J]. Chinese Journal of Animal Nutrition, 2020, 32(5): 2379-2386. (in Chinese with English abstract)
[15]	FU H T, JIANG S F, XIONG Y W. Current status and prospects of farming the giant river prawn (Macrobrachium rosenbergii) and the oriental river prawn (Macrobrachium nipponense) in China[J]. Aquaculture Research, 2012, 43(7): 993-998.
[16]	MIAO W M, GE X P. Freshwater prawn culture in China: an overview[J]. Journal of Aquaculture Asia, 2002, 7(1): 9-12.
[17]	唐金玉, 覃宝利, 叶建勇, 等. 江苏泗阳青虾养殖中期水体的理化环境和浮游植物[J]. 渔业现代化, 2019, 46(3): 41-51.
	TANG J Y, QIN B L, YE J Y, et al. Physicochemical environment and phytoplankton in Macrobrachium nipponense mid-cultured waters in Siyang City, Jiangsu Province[J]. Fishery Modernization, 2019, 46(3): 41-51. (in Chinese with English abstract)
[18]	唐金玉, 叶建勇, 戴杨鑫, 等. 江苏日本沼虾4个野生种群肌肉营养成分比较[J]. 水生生物学报, 2021, 45(4): 801-808.
	TANG J Y, YE J Y, DAI Y X, et al. Comparison of muscle nutritional compositions of four wild oriental river prawn Macrobrachium nipponense populations in Jiangsu Province[J]. Acta Hydrobiologica Sinica, 2021, 45(4): 801-808. (in Chinese with English abstract)
[19]	殷艳慧, 蒋万胜, 潘晓赋, 等. 水产养殖鱼类生长性状研究进展[J]. 中国水产科学, 2020, 27(4): 463-484.
	YIN Y H, JIANG W S, PAN X F, et al. Recent progress in growth trait of aquaculture fish[J]. Journal of Fishery Sciences of China, 2020, 27(4): 463-484. (in Chinese with English abstract)
[20]	IRWIN S, O’HALLORAN J, FITZGERALD R D. Stocking density, growth and growth variation in juvenile turbot, Scophthalmus maximus(Rafinesque)[J]. Aquaculture, 1999, 178(1/2): 77-88.
[21]	倪娟, 赵晓勤, 陈立侨, 等. 日本沼虾4种群肌肉营养品质的比较[J]. 中国水产科学, 2003, 10(3): 212-215.
	NI J, ZHAO X Q, CHEN L Q, et al. A comparison of nutritional quality in muscle of Macrobrachium nipponensis from four populations[J]. Journal of Fishery Sciences of China, 2003, 10(3): 212-215. (in Chinese with English abstract)
[22]	吴滟, 龚永生, 熊贻伟, 等. 杂交青虾“太湖1号”肌肉营养成分分析[J]. 淡水渔业, 2011, 41(1): 73-77.
	WU Y, GONG Y S, XIONG Y W, et al. Evaluation of nutritive quality and nutritional composition of muscle in hybrid oriental river prawns named “Taihu No.1”[J]. Freshwater Fisheries, 2011, 41(1): 73-77. (in Chinese with English abstract)
[23]	刘肖莲, 姜巨峰, 吴会民, 等. 滨海型盐碱水域杂交青虾“太湖1号”肌肉营养成分分析[J]. 渔业现代化, 2017, 44(5): 67-72.
	LIU X L, JIANG J F, WU H M, et al. Analysis of nutritional components in the muscle of hybrid oriental river prawn “Taihu No.1” cultured in coastal saline-alkaline pond[J]. Fishery Modernization, 2017, 44(5): 67-72. (in Chinese with English abstract)
[24]	范武江, 苏明, 李雪松, 等. 日本沼虾淀山湖野生群体和养殖群体肌肉营养成分分析[J]. 水产科技情报, 2019, 46(4): 181-186.
	FAN W J, SU M, LI X S, et al. Analysis of nutritive components in muscle of Macrobrachium nipponense between the wild and cultured populations in Dianshan Lake[J]. Fisheries Science & Technology Information, 2019, 46(4): 181-186. (in Chinese with English abstract)
[25]	唐雪, 徐钢春, 徐跑, 等. 野生与养殖刀鲚肌肉营养成分的比较分析[J]. 动物营养学报, 2011, 23(3): 514-520.
	TANG X, XU G C, XU P, et al. A comparison of muscle nutrient composition between wild and cultured Coilia nasus[J]. Chinese Journal of Animal Nutrition, 2011, 23(3): 514-520. (in Chinese with English abstract)
[26]	吴丹, 江敏, 吴昊, 等. 大棚养殖和露天养殖模式下不同生长阶段凡纳滨对虾肌肉营养成分比较[J]. 上海海洋大学学报, 2019, 28(4): 491-500.
	WU D, JIANG M, WU H, et al. Comparison of nutritional component of greenhouse cultured and outdoor cultured Litopenaeus vannamei in different growth stages[J]. Journal of Shanghai Ocean University, 2019, 28(4): 491-500. (in Chinese with English abstract)
[27]	韩晓磊, 王浩, 冯一凡, 等. 不同养殖模式下加州鲈营养成分差异分析[J]. 淡水渔业, 2020, 50(3): 87-93.
	HAN X L, WANG H, FENG Y F, et al. Comparative study on nutritional components of Micropterus salmoides under different aquaculture modes[J]. Freshwater Fisheries, 2020, 50(3): 87-93. (in Chinese with English abstract)
[28]	ZHANG N N, MA Q Q, FAN W J, et al. Effects of the dietary protein to energy ratio on growth, feed utilization and body composition in Macrobrachium nipponense[J]. Aquaculture Nutrition, 2017, 23(2): 313-321.
[29]	张升利, 张欣, 孙向军, 等. 投喂不同饵料对吉富罗非鱼肌肉营养成分的影响[J]. 农业生物技术学报, 2013, 21(10): 1210-1220.
	ZHANG S L, ZHANG X, SUN X J, et al. Effects of different diets on nutritive components in the muscle of genetic improvement of farmed Tilapia[J]. Journal of Agricultural Biotechnology, 2013, 21(10): 1210-1220. (in Chinese with English abstract)
[30]	施永海, 张根玉, 刘永士, 等. 野生及养殖哈氏仿对虾肌肉营养成分的分析与比较[J]. 水产学报, 2013, 37(5): 768-776.
	SHI Y H, ZHANG G Y, LIU Y S, et al. Comparison of muscle nutrient composition between wild and cultured sword prawn (Parapenaeopsis hardwickii)[J]. Journal of Fisheries of China, 2013, 37(5): 768-776. (in Chinese with English abstract)
[31]	LAVAJOO F, AMROLLAHI BIUKI N, KHANIPOUR A, et al. An invasive shrimp species, Macrobrachium nipponense, in anzali wetland demonstrated a potential source for commercial fishing[J]. Journal of Aquatic Food Product Technology, 2018, 27(9): 975-985.
[32]	LUO N, DING Z L, KONG Y Q, et al. An evaluation of increasing linolenic acid level in the diet of Macrobrachium nipponense: lipid deposition, fatty acid composition and expression of lipid metabolism-related genes[J]. Aquaculture Nutrition, 2018, 24(2): 758-767.
[33]	CAHU C, SALEN P, DE LORGERIL M. Farmed and wild fish in the prevention of cardiovascular diseases: assessing possible differences in lipid nutritional values[J]. Nutrition, Metabolism and Cardiovascular Diseases, 2004, 14(1): 34-41.
[34]	TSAPE K, SINANOGLOU V J, MINIADIS-MEIMAROGLOU S. Comparative analysis of the fatty acid and sterol profiles of widely consumed Mediterranean crustacean species[J]. Food Chemistry, 2010, 122(1): 292-299.
[35]	AYAS D, OZOGUL Y, YAZGAN H. The effects of season on fat and fatty acids contents of shrimp and prawn species[J]. European Journal of Lipid Science and Technology, 2013, 115(3): 356-362.

性别 Gender	处理 Treatment	全长 Total length/cm	头胸甲长 Carapace length/cm	体长 Body length/cm	体重 Wet weight/g
雄Male	T1	5.19±0.16 c	0.63±0.03 c	4.55±0.14 c	1.58±0.15 c
	T2	6.71±0.16 b	0.81±0.04 b	5.90±0.15 b	4.54±0.25 b
	T3	7.68±0.15 a	1.07±0.04 a	6.62±0.12 a	5.47±0.25 a
雌Female	T1	5.08±0.06 c	0.54±0.02 c	4.54±0.06 c	1.87±0.07 c
	T2	6.55±0.10 a	0.73±0.03 a	5.82±0.09 a	5.16±0.19 a
	T3	5.70±0.11 b	0.66±0.03 b	5.04±0.11 b	2.89±0.20 b

性别 Gender	处理 Treatment	全长 Total length/cm	头胸甲长 Carapace length/cm	体长 Body length/cm	体重 Wet weight/g
雄Male	T1	5.19±0.16 c	0.63±0.03 c	4.55±0.14 c	1.58±0.15 c
	T2	6.71±0.16 b	0.81±0.04 b	5.90±0.15 b	4.54±0.25 b
	T3	7.68±0.15 a	1.07±0.04 a	6.62±0.12 a	5.47±0.25 a
雌Female	T1	5.08±0.06 c	0.54±0.02 c	4.54±0.06 c	1.87±0.07 c
	T2	6.55±0.10 a	0.73±0.03 a	5.82±0.09 a	5.16±0.19 a
	T3	5.70±0.11 b	0.66±0.03 b	5.04±0.11 b	2.89±0.20 b

处理 Treatment	粗蛋白含量 Crude protein content	粗脂肪含量 Crude lipid content	粗灰分含量 Ash content	水分含量 Moisture content
T1	17.94±0.42	2.25±0.01	1.12±0.01 a	78.69±0.41
T2	17.97±0.43	2.19±0.16	0.95±0.03 b	78.89±0.82
T3	16.54±0.24	1.86±0.11	1.04±0.01 ab	80.56±1.13

处理 Treatment	粗蛋白含量 Crude protein content	粗脂肪含量 Crude lipid content	粗灰分含量 Ash content	水分含量 Moisture content
T1	17.94±0.42	2.25±0.01	1.12±0.01 a	78.69±0.41
T2	17.97±0.43	2.19±0.16	0.95±0.03 b	78.89±0.82
T3	16.54±0.24	1.86±0.11	1.04±0.01 ab	80.56±1.13

指标 Index	各处理下的含量Content under different treatments
指标 Index	T1	T2	T3
赖氨酸Lysine	7.42±0.23	7.37±0.16	7.22±0.03
苯丙氨酸Phenylalanine	3.55±0.05	3.53±0.07	3.44±0.05
苏氨酸Threonine	3.25±0.04	3.22±0.07	3.15±0.01
异亮氨酸Isoleucine	3.87±0.06	3.82±0.07	3.74±0.04
亮氨酸Leucine	6.44±0.13	6.36±0.14	6.20±0.01
缬氨酸Valine	3.74±0.06	3.78±0.08	3.67±0.01
蛋氨酸Methionine	2.54±0.03	2.48±0.05	2.51±0.03
组氨酸Histidine	1.87±0.10	1.96±0.06	1.97±0.01
精氨酸Arginine^△	5.50±0.20	6.10±0.16	6.29±0.38
天冬氨酸Aspartic acid^*	8.66±0.27	8.69±0.27	8.41±0.02
丙氨酸Alanine^*△	5.85±0.00 a	5.82±0.02 a	5.59±0.00 b
谷氨酸Glutamate^*	13.56±0.27	13.21±0.30	12.81±0.11
甘氨酸Glycine^*△	5.17±0.67	4.59±0.04	4.55±0.32
丝氨酸Serine	3.13±0.07	3.15±0.10	3.10±0.01
半胱氨酸Cysteine^△	1.58±0.17	1.34±0.09	1.42±0.07
脯氨酸Proline	2.94±0.03	3.32±0.10	3.05±0.15
酪氨酸Tyrosine	3.05±0.11	3.12±0.06	3.03±0.00
总氨基酸(TAA)含量Total amino acids (TAA) content	82.13±2.48	81.85±1.86	80.14±0.39
必需氨基酸(EAA)总量Essential amino acids (EAA) content	28.28±0.57	28.08±0.58	27.41±0.13
非必需氨基酸(NEAA)总量Non-essential amino acids (NEAA) content	46.47±1.61	45.72±1.06	44.47±0.63
鲜味氨基酸(DAA)总量Delicious amino acids (DAA) content	33.24±1.20	32.31±0.64	31.36±0.42
虾味氨基酸(SAA)总量Shrimp flavor amino acids (SAA) content	18.10±1.03	17.85±0.31	17.85±0.02
(EAA/TAA)/%	34.45±0.34	34.30±0.07	34.20±0.00
(EAA/NEAA)/%	60.89±0.88	61.41±0.15	61.64±0.59

Effects of stocking modes on growth traits and muscle nutritional composition of Macrobrachium nipponense

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氨基酸 Amino acid	FAO模式评分 Score based on the FAO model/ (mg·g^-1)	鸡蛋蛋白模式评分 Score based on the egg model/ (mg·g^-1)	各处理的氨基酸评分(AAS) Amino acid score (AAS) of treatments			各处理的化学评分(CS) Chemical score (CS) of treatments
氨基酸 Amino acid	FAO模式评分 Score based on the FAO model/ (mg·g^-1)	鸡蛋蛋白模式评分 Score based on the egg model/ (mg·g^-1)	T1	T2	T3	T1	T2	T3
异亮氨酸 Isoleucine	2.50	3.31	0.97 ±0.02	0.95 ±0.02	0.93 ±0.01	0.73 ±0.01	0.72 ±0.01	0.71 ±0.01
亮氨酸 Leucine	4.40	5.34	0.92 ±0.02	0.90 ±0.02	0.88 ±0.00	0.75 ±0.02	0.74 ±0.02	0.73 ±0.00
赖氨酸 Lysine	3.40	4.41	1.36 ±0.04	1.35 ±0.03	1.33 ±0.01	1.05 ±0.03	1.04 ±0.02	1.02 ±0.00
苏氨酸 Threonine	2.50	2.92	0.81 ±0.01	0.80 ±0.02	0.79 ±0.00	0.70 ±0.01	0.69 ±0.01	0.67 ±0.00
缬氨酸 Valine	3.10	4.10	0.75 ±0.01	0.76 ±0.02	0.74 ±0.00	0.57 ±0.01	0.58 ±0.01	0.56 ±0.00
蛋氨酸-半胱氨酸 Methionine-cysteine	2.20	3.86	1.17 ±0.06	1.09 ±0.04	1.12 ±0.01	0.67 ±0.03	0.62 ±0.02	0.64 ±0.01
苯丙氨酸-酪氨酸 Phenylalanine-tyrosine	3.80	5.65	1.09 ±0.03	1.09 ±0.02	1.06 ±0.01	0.73 ±0.02	0.73 ±0.01	0.72 ±0.01

指标 Index	各处理的含量Content under treatments
指标 Index	T1	T2	T3
C14:0/%	2.35±0.08	1.61±0.08	3.52±0.62
C16:0/%	24.25±0.60	19.24±0.29	24.16±2.37
C18:0/%	9.56±0.45	8.85±0.49	9.36±0.29
C20:0/%	—	—	0.34±0.03
C22:0/%	14.09±2.24	13.83±1.18	14.33±1.16
C24:0/%	1.27±0.02	1.12±0.04	1.67±0.21
SFA/%	51.51±2.19	44.65±0.37	53.21±2.08
C14:1/%	—	—	0.48±0.07
C16:1/%	5.60±0.21	3.53±0.53	5.71±0.01
C18:1/%	15.58±3.20	17.93±1.33	18.00±2.45
C20:1/%	0.54±0.04	0.68±0.11	0.52±0.03
MUFA/%	18.64±2.67	22.13±1.75	21.62±0.20
C18:2 n-6/%	11.13±0.77 a	10.50±0.84 a	4.78±0.15 b
C18:3 n-3/%	3.62±0.21	2.83±0.23	3.75±0.71
C20:2 n-6/%	0.98±0.04	0.91±0.03	0.83±0.09
C20:4 n-6/%	0.56±0.10	0.46±0.06	0.41±0.03
C20:5 n-3(EPA)/%	9.82±0.91	8.63±1.36	8.63±1.14
C22:6 n-3(DHA)/%	4.55±1.28	3.46±0.56	4.56±0.61
PUFA/%	29.90±0.87 a	26.79±0.76 a	22.10±0.52 b
EPA+DHA/%	14.37±2.19	12.09±1.92	13.19±1.75
Σn-3 PUFAs/%	17.99±2.40	14.91±1.69	16.94±1.04
Σn-6 PUFAs/%	11.90±1.53 a	11.87±0.93 a	5.16±0.53 b
总脂肪酸含量Total fatty acids content/%	98.05±3.22	93.57±0.62	96.93±1.37
Σn-3 PUFAs/Σn-6 PUFAs	1.56±0.40	1.28±0.24	3.34±0.54
AI	0.70±0.07	0.53±0.00	0.88±0.13
TI	0.51±0.03	0.47±0.04	0.55±0.08

处理 Treatment	K含量 K content	Na含量 Na content	Ca含量 Ca content	Mg含量 Mg content	Mn含量 Mn content	Zn含量 Zn content	Fe含量 Fe content	Cr含量 Cr content	Cu含量 Cu content
T1	13 255.3 ±215.5	6 520.4 ±10.1 a	4 835.7 ±197.5	1 307.4 ±89.6	9.15 ±0.05 a	56.55 ±1.55 b	33.15 ±1.75 ab	0.029 ±0.009	16.32 ±0.45
T2	14 570.2 ±160.3	6 375.2 ±105.5 ab	4 584.4 ±219.7	1 239.3 ±28.1	5.39 ±0.05 b	63.26 ±0.05 a	43.63 ±0.91 a	0.034 ±0.007	13.34 ±1.78
T3	15 804.8 ±475.7	5 315.2 ±15.2 b	4 648.3 ±189.2	1 176.5 ±18.9	3.27 ±0.53 c	63.27 ±1.21 a	18.12 ±4.53 b	0.018 ±0.005	15.25 0.21

[1]	PENG Jiacheng, WU Yue, XU Jiehao, XIA Meiwen, QI Tianpeng, XU Haisheng. Cloning of paxillin gene from Macrobrachium nipponense and effect of cadmium stress on its expression [J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 247-253.
[2]	WANG Fei, HAN Yujie, FANG Yi, XIANG Hai, CHANG Xiao, ZHONG Rongzhen. Evaluation on nutritional value of Flammulina filiformis cultivation substrate with different cultivation time [J]. Acta Agriculturae Zhejiangensis, 2024, 36(1): 75-83.
[3]	DONG Zhihao, CHEN Yu, HUANG Gaoxiang, BAI Junyan, LI Jingyun, ZHAO Shujuan, LEI Ying, WANG Xinle, HU Qihang, FAN Zhengyu. Association analysis of VIPR-1 gene polymorphism and early growth traits in egg quail [J]. Acta Agriculturae Zhejiangensis, 2021, 33(8): 1393-1401.
[4]	YU Shijun, HE Lingyan, CHENG Ming, XU Xin, XU Xinyi, CHAI Xinyi, WANG Weikun. Effect of selenium on nutritional and functional components of synnemata of Isaria cicadae [J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2245-2253.
[5]	WU Yi-fei, SUN Hong, LI Yuan-cheng, WANG Xin, LIU Yong, YAO Xiao-hong, TANG Jiang-wu. Effects of microbial solid-state fermentation on nutritional value of feeds [J]. , 2016, 28(12): 2014-2020.