Effect of sublethal dosage of fenvalerate to growth and development of the silkworm, Bombyx mori

doi:10.3969/j.issn.1004-1524.20230268

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (2): 264-271.DOI: 10.3969/j.issn.1004-1524.20230268

• Animal Science • Previous Articles Next Articles

Effect of sublethal dosage of fenvalerate to growth and development of the silkworm, Bombyx mori

YU Ruixian¹^,²(), HU Xiuqing¹^,², LIU Xinju¹^,², TANG Tao¹^,², WU Min¹^,², WU Shenggan¹^,², ZHAO Xueping¹^,²^,^*()

1. Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. State Key Laboratory for Managing Biotic and Chemical Threats to Quality and Safety of Agro-Products, Hangzhou 310021, China

Received:2023-03-06 Online:2024-02-25 Published:2024-03-05

Abstract

Abstract:

In order to provide reference for choosing pesticides in controlling pests in agro-mulberry mixed planting areas, the acute toxicity and developmental toxicity of fenvalerate to silkworm were investigated by leaf dipping method. The results of acute toxicity showed that the 96 h-LC₅₀ for the 2nd instar larvae to fenvalerate was 0.251-0.570 mg·L^-1, belonging to high and extreme level toxicity. The results of developmental toxicity showed that after the treatments at the sublethal dosages (0.062 8, 0.020 9, 0.006 98, 0.002 33 and 0.000 775 mg·L^-1) of fenvalerate, the development duration and body weight of molting larvae were affected, and the influence gradually became less abvious with instar. The rate of pupation and dead cocoons of 0.002 33 and 0.000 775 mg·L^-1 fenvalerate treatment groups were significantly affected (P<0.05), which significantly affected the individual growth and development. The cocoon shell weight of 0.062 8, 0.020 9 and 0.006 98 mg·L^-1 fenvalerate treatment groups were significantly lower than that of the control group (P<0.05). Results showed that fenvalerate had high acute and chronic toxic effects on silkworms. Therefore, when using fenvalerate in agro-mulberry mixed planting areas, mulberry pollution from droplet drift or other agricultural activities should be avoided.

Key words: fenvalerate, Bombyx mori, sublethal effect, growth and developmental toxicity

CLC Number:

S482.35

YU Ruixian, HU Xiuqing, LIU Xinju, TANG Tao, WU Min, WU Shenggan, ZHAO Xueping. Effect of sublethal dosage of fenvalerate to growth and development of the silkworm, Bombyx mori[J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 264-271.

Figures/Tables 5

References 43

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家蚕品种 Silkworm variety	时间 Time/h	毒力回归曲线 Toxicity regressive equation	LC₅₀/(mg·L^-1)	95%可置信限 95% FI/(mg·L^-1)	R²
芙蓉Furong	72	Y=5.807+6.249X	0.743	0.687~0.828	0.896
	96	Y=7.137+8.757X	0.570	0.469~0.667	0.905
湘辉Xianghui	72	Y=10.974+13.038X	0.348	0.315~0.411	0.893
	96	Y=11.415+13.085X	0.323	0.299~0.366	0.918
皓月Haoyue	72	Y=8.406+6.491X	0.299	0.257~0.331	0.958
	96	Y=8.745+6.239X	0.251	0.179~0.299	0.916

家蚕品种 Silkworm variety	时间 Time/h	毒力回归曲线 Toxicity regressive equation	LC₅₀/(mg·L^-1)	95%可置信限 95% FI/(mg·L^-1)	R²
芙蓉Furong	72	Y=5.807+6.249X	0.743	0.687~0.828	0.896
	96	Y=7.137+8.757X	0.570	0.469~0.667	0.905
湘辉Xianghui	72	Y=10.974+13.038X	0.348	0.315~0.411	0.893
	96	Y=11.415+13.085X	0.323	0.299~0.366	0.918
皓月Haoyue	72	Y=8.406+6.491X	0.299	0.257~0.331	0.958
	96	Y=8.745+6.239X	0.251	0.179~0.299	0.916

氰戊菊酯浓度 Fenvalerate concentration/(mg·L^-1)	幼虫历期 Larval duration/h
氰戊菊酯浓度 Fenvalerate concentration/(mg·L^-1)	2龄2nd instar	3龄3rd instar	4龄4th instar	5龄5th instar
0.062 8	75.33±0.63 c	87.67±2.41 ab	100.07±3.01 ab	173.63±2.13 a
0.020 9	76.67±0.93 c	85.80±4.17 ab	101.73±1.40 a	180.37±3.20 a
0.006 98	79.83±4.49 bc	83.30±3.72 b	101.00±1.23 a	176.53±6.65 a
0.002 33	77.90±2.77 c	85.73±1.42 ab	100.03±1.72 ab	172.80±1.04 a
0.000 775	76.97±1.69 c	90.57±1.35 a	96.37±0.894 b	173.97±2.86 a
0(空白Control)	85.70±3.12 a	83.17±3.55 b	103.13±2.67 a	172.87±4.08 a
0(助剂Adjuvant)	83.30±1.99 ab	83.80±1.77 b	100.43±1.14 a	176.30±4.09 a

氰戊菊酯浓度 Fenvalerate concentration/(mg·L^-1)	幼虫历期 Larval duration/h
氰戊菊酯浓度 Fenvalerate concentration/(mg·L^-1)	2龄2nd instar	3龄3rd instar	4龄4th instar	5龄5th instar
0.062 8	75.33±0.63 c	87.67±2.41 ab	100.07±3.01 ab	173.63±2.13 a
0.020 9	76.67±0.93 c	85.80±4.17 ab	101.73±1.40 a	180.37±3.20 a
0.006 98	79.83±4.49 bc	83.30±3.72 b	101.00±1.23 a	176.53±6.65 a
0.002 33	77.90±2.77 c	85.73±1.42 ab	100.03±1.72 ab	172.80±1.04 a
0.000 775	76.97±1.69 c	90.57±1.35 a	96.37±0.894 b	173.97±2.86 a
0(空白Control)	85.70±3.12 a	83.17±3.55 b	103.13±2.67 a	172.87±4.08 a
0(助剂Adjuvant)	83.30±1.99 ab	83.80±1.77 b	100.43±1.14 a	176.30±4.09 a

氰戊菊酯浓度 Fenvalerate concentration/ (mg·L^-1)	全茧量 Cocoon weight/g	茧层量 Cocoon shell weight/g	蛹重 Pupa weight/g	幼虫死亡率 Larvae mortality/%	结茧率 Cocooning rate/%	化蛹率 Pupation rate/%	死笼率 Rate of dead cocoon/%
0.062 8	1.660±0.044 1 a	0.335±0.005 8 b	1.316±0.025 1 a	4.44 a	95.56 a	83.76 bc	16.24 bc
0.020 9	1.656±0.014 9 a	0.335±0.008 1 b	1.311±0.030 1 a	2.22 a	97.78 a	88.73 abc	11.27 bcd
0.006 98	1.687±0.025 3 a	0.339±0.013 1 b	1.339±0.020 7 a	6.67 a	93.33 a	88.39 abc	11.61 bcd
0.002 33	1.727±0.078 2 a	0.355±0.016 5 ab	1.362±0.055 5 a	4.44 a	95.56 a	79.21 c	18.41 b
0.000 775	1.691±0.037 1 a	0.356±0.014 4 ab	1.325±0.038 7 a	4.44 a	95.56 a	64.92 d	35.08 a
0(空白Control)	1.760±0.016 6 a	0.366±0.002 6 a	1.387±0.020 1 a	2.22 a	97.78 a	95.56 a	4.44 d
0(助剂Adjuvant)	1.703±0.146 4 a	0.363±0.010 0 a	1.339±0.153 9 a	2.22 a	97.78 a	93.17 ab	6.83 cd

Effect of sublethal dosage of fenvalerate to growth and development of the silkworm, Bombyx mori

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