Effect of selenium on nutritional and functional components of synnemata of Isaria cicadae

doi:10.3969/j.issn.1004-1524.2021.12.04

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (12): 2245-2253.DOI: 10.3969/j.issn.1004-1524.2021.12.04

• Crop Science • Previous Articles Next Articles

Effect of selenium on nutritional and functional components of synnemata of Isaria cicadae

YU Shijun¹(), HE Lingyan¹, CHENG Ming¹, XU Xin¹, XU Xinyi¹, CHAI Xinyi¹, WANG Weikun²

1. School of Biology and Food Engineering, Chuzhou University, Chuzhou 239000, China
2. General Biosystem (Anhui) Co., Ltd., Chuzhou 239000, China

Received:2020-08-18 Online:2021-12-25 Published:2022-01-10

Abstract

Abstract:

The effect of selenium on the the nutrition and functional components in the synnemata of Isaria cicadae was explored by the selenium-enriched culture of synnemata of I. cicadae using sodium selenite as the source of selenium. And the nutritional value of the protein of synnemata of I. cicadae was evaluated. The results showed that the selenium amount of synnemata increased with the increase of sodium selenite in the culture media. When the concentration of sodium selenite was 100 mg·kg ^-1 in the medium, the selenium content in synnemata of I. cicadae reached 142.22 mg·kg ^-1. The contents of protein, fat, adenosine and mannitol in the selenium-enriched synnemata of I. cicadae were significantly higher than those of the control group. The amino acid ratio coefficient score (SRCAA) of protein of synnemata of I. cicadae, cultured in the media with 75 mg·kg ^-1 sodium selenite, was 52.60, which is higher than the control group. Cluster analysis indicated that, in general, the effect of sodium selenite on the nutritional and functional components of synnemata of I. cicadae were increased with the increase of sodium selenite in the culture media. It can be concluded that when sodium selenite was 75 mg·kg ^-1 in the media, it could not only increase the contents of protein, fat, adenosine and mannitol in synnemata of I. cicadae, but also increase its protein nutritional value.

Key words: Isaria cicadae, synnemata, selenium, nutritional value, functional components

CLC Number:

S567

YU Shijun, HE Lingyan, CHENG Ming, XU Xin, XU Xinyi, CHAI Xinyi, WANG Weikun. Effect of selenium on nutritional and functional components of synnemata of Isaria cicadae[J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2245-2253.

Figures/Tables 6

References 47

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成分含量 Component content	对照组 Control	Na₂SeO₃/(mg·kg^-1)
成分含量 Component content	对照组 Control	50	75	100
硒含量Selenium content/(mg·kg^-1)	0.18±0.03 d	83.62±3.74 c	115.61±4.89 b	142.22±5.41 a
腺苷Adenosine/(mg·g^-1)	1.18±0.05 b	1.31±0.06 a	1.42±0.07 a	1.41±0.04 a
甘露醇Mannitol/(mg·g^-1)	53.15±5.25 c	52.11±5.30 c	66.56±5.87 b	78.63±4.97 a
脂肪Fat/%	3.24±0.49 c	3.52±0.51 c	5.56±0.83 b	6.68±0.43 a
多糖Polysaccharide/%	15.16±1.94 a	15.77±3.08 a	11.17±0.16 b	8.93±0.69 b
蛋白质Protein/%	29.78±0.54 d	34.47±0.68 b	31.57±0.29 c	35.92±0.75 a

成分含量 Component content	对照组 Control	Na₂SeO₃/(mg·kg^-1)
成分含量 Component content	对照组 Control	50	75	100
硒含量Selenium content/(mg·kg^-1)	0.18±0.03 d	83.62±3.74 c	115.61±4.89 b	142.22±5.41 a
腺苷Adenosine/(mg·g^-1)	1.18±0.05 b	1.31±0.06 a	1.42±0.07 a	1.41±0.04 a
甘露醇Mannitol/(mg·g^-1)	53.15±5.25 c	52.11±5.30 c	66.56±5.87 b	78.63±4.97 a
脂肪Fat/%	3.24±0.49 c	3.52±0.51 c	5.56±0.83 b	6.68±0.43 a
多糖Polysaccharide/%	15.16±1.94 a	15.77±3.08 a	11.17±0.16 b	8.93±0.69 b
蛋白质Protein/%	29.78±0.54 d	34.47±0.68 b	31.57±0.29 c	35.92±0.75 a

氨基酸种类 Amino type	对照组 Control	Na₂SeO₃/(mg·kg^-1)
氨基酸种类 Amino type	对照组 Control	50	75	100
天冬氨酸Asp	1.12	1.09	1.74	1.75
苏氨酸Thr^*	1.35	1.46	1.24	1.38
丝氨酸Ser	1.10	1.22	1.08	1.15
谷氨酸Glu	3.37	2.74	2.23	2.38
甘氨酸Gly	0.89	1.05	1.04	1.14
丙氨酸Ala	1.42	1.60	1.54	1.58
半胱氨酸Cys^*	0.46	0.51	0.34	0.43
缬氨酸Val^*	0.98	0.95	0.95	0.96
蛋氨酸Met^*	1.47	1.82	1.36	1.79
异亮氨酸Ile^*	0.75	0.75	0.78	0.79
亮氨酸Leu^*	1.31	1.25	1.32	1.33
酪氨酸Tyr	1.96	1.80	1.74	1.97
苯丙氨酸Phe^*	0.96	0.94	0.96	1.01
赖氨酸Lys^*	1.01	0.94	0.92	1.03
组氨酸His	0.46	0.44	0.45	0.46
精氨酸Arg	1.14	1.22	1.16	1.30
脯氨酸Pro	1.77	1.41	1.46	1.51
必需氨基酸EAA	7.81	8.11	7.52	8.29
总氨基酸TAA	21.49	21.18	20.29	21.96
EAA/TAA	36.35	38.27	37.08	37.76

氨基酸种类 Amino type	对照组 Control	Na₂SeO₃/(mg·kg^-1)
氨基酸种类 Amino type	对照组 Control	50	75	100
天冬氨酸Asp	1.12	1.09	1.74	1.75
苏氨酸Thr^*	1.35	1.46	1.24	1.38
丝氨酸Ser	1.10	1.22	1.08	1.15
谷氨酸Glu	3.37	2.74	2.23	2.38
甘氨酸Gly	0.89	1.05	1.04	1.14
丙氨酸Ala	1.42	1.60	1.54	1.58
半胱氨酸Cys^*	0.46	0.51	0.34	0.43
缬氨酸Val^*	0.98	0.95	0.95	0.96
蛋氨酸Met^*	1.47	1.82	1.36	1.79
异亮氨酸Ile^*	0.75	0.75	0.78	0.79
亮氨酸Leu^*	1.31	1.25	1.32	1.33
酪氨酸Tyr	1.96	1.80	1.74	1.97
苯丙氨酸Phe^*	0.96	0.94	0.96	1.01
赖氨酸Lys^*	1.01	0.94	0.92	1.03
组氨酸His	0.46	0.44	0.45	0.46
精氨酸Arg	1.14	1.22	1.16	1.30
脯氨酸Pro	1.77	1.41	1.46	1.51
必需氨基酸EAA	7.81	8.11	7.52	8.29
总氨基酸TAA	21.49	21.18	20.29	21.96
EAA/TAA	36.35	38.27	37.08	37.76

氨基酸 Amino acid/(mg·g^-1)	鸡蛋模式 Egg mode	FAO/WHO模式 FAO/WHO mode	对照组 Control	Na₂SeO₃/(mg·kg^-1)
氨基酸 Amino acid/(mg·g^-1)	鸡蛋模式 Egg mode	FAO/WHO模式 FAO/WHO mode	对照组 Control	50	75	100
异亮氨酸Ile	54	40	25.16	21.77	24.77	22.06
亮氨酸Leu	86	70	43.95	36.34	41.83	37.14
赖氨酸Lys	70	55	33.87	27.20	29.12	28.61
蛋氨酸+半胱氨酸Met+Cys	57	35	64.78	67.59	53.90	67.75
苯丙氨酸+酪氨酸Phe+Tyr	93	60	98.10	79.60	85.46	83.19
苏氨酸Thr	47	40	45.22	42.28	39.14	38.33
缬氨酸Val	66	50	32.74	27.54	30.01	26.80
总必需氨基酸TEAA	473	350	343.82	302.33	304.22	297.88

Effect of selenium on nutritional and functional components of synnemata of Isaria cicadae

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处理 Treatment		必需氨基酸指数 EAAI	生物价 BV	营养指数 NI
对照组Control		73.01	67.88	21.74
Na₂SeO₃/	50	65.33	59.51	22.52
(mg·kg^-1)	75	64.47	58.57	20.35
	100	63.55	57.57	22.83

氨基酸 Amino acid	对照组 Control		Na₂SeO₃/(mg·kg^-1)
	对照组 Control		50		75		100
	RAA	RCAA	RAA	RCAA	RAA	RCAA	RAA	RCAA
异亮氨酸Ile	0.63	0.62	0.54	0.59	0.62	0.69	0.55	0.62
亮氨酸Leu	0.63	0.62	0.52	0.57	0.60	0.67	0.53	0.59
赖氨酸Lys	0.62	0.60	0.49	0.54	0.53	0.59	0.52	0.58
蛋氨酸+半胱氨酸Met+Cys	1.85	1.81	1.93	2.10	1.54	1.71	1.76	1.98
苯丙氨酸+酪氨酸Phe+Tyr	1.63	1.60	1.33	1.45	1.42	1.59	1.39	1.55
苏氨酸Thr	1.13	1.11	1.06	1.15	0.98	1.09	0.96	1.07
缬氨酸Val	0.65	0.64	0.55	0.60	0.60	0.67	0.54	0.60
均数Average value	1.02	1.00	0.92	1.00	0.90	1.00	0.89	1.00
SRCAA	48.09		39.89		52.60		43.58

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