Effect of selenium on nutritional and functional components of synnemata of Isaria cicadae

doi:10.3969/j.issn.1004-1524.2021.12.04

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (12): 2245-2253.DOI: 10.3969/j.issn.1004-1524.2021.12.04

• Crop Science • Previous Articles Next Articles

Effect of selenium on nutritional and functional components of synnemata of Isaria cicadae

YU Shijun¹(), HE Lingyan¹, CHENG Ming¹, XU Xin¹, XU Xinyi¹, CHAI Xinyi¹, WANG Weikun²

1. School of Biology and Food Engineering, Chuzhou University, Chuzhou 239000, China
2. General Biosystem (Anhui) Co., Ltd., Chuzhou 239000, China

Received:2020-08-18 Online:2021-12-25 Published:2022-01-10

Abstract

Abstract:

The effect of selenium on the the nutrition and functional components in the synnemata of Isaria cicadae was explored by the selenium-enriched culture of synnemata of I. cicadae using sodium selenite as the source of selenium. And the nutritional value of the protein of synnemata of I. cicadae was evaluated. The results showed that the selenium amount of synnemata increased with the increase of sodium selenite in the culture media. When the concentration of sodium selenite was 100 mg·kg ^-1 in the medium, the selenium content in synnemata of I. cicadae reached 142.22 mg·kg ^-1. The contents of protein, fat, adenosine and mannitol in the selenium-enriched synnemata of I. cicadae were significantly higher than those of the control group. The amino acid ratio coefficient score (SRCAA) of protein of synnemata of I. cicadae, cultured in the media with 75 mg·kg ^-1 sodium selenite, was 52.60, which is higher than the control group. Cluster analysis indicated that, in general, the effect of sodium selenite on the nutritional and functional components of synnemata of I. cicadae were increased with the increase of sodium selenite in the culture media. It can be concluded that when sodium selenite was 75 mg·kg ^-1 in the media, it could not only increase the contents of protein, fat, adenosine and mannitol in synnemata of I. cicadae, but also increase its protein nutritional value.

Key words: Isaria cicadae, synnemata, selenium, nutritional value, functional components

CLC Number:

S567

YU Shijun, HE Lingyan, CHENG Ming, XU Xin, XU Xinyi, CHAI Xinyi, WANG Weikun. Effect of selenium on nutritional and functional components of synnemata of Isaria cicadae[J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2245-2253.

Figures/Tables 6

References 47

[1]	LI I C, LIN S, TSAI Y T, et al. Cordyceps cicadae mycelia and its active compound HEA exert beneficial effects on blood glucose in type 2 diabetic db/db mice[J]. Journal of the Science of Food and Agriculture, 2019, 99(2): 606-612. DOI PMID
[2]	LI L, ZHANG T, LI C R, et al. Potential therapeutic effects of Cordyceps cicadae and Paecilomyces cicadae on adenine-induced chronic renal failure in rats and their phytochemical analysis[J]. Drug Design, Development and Therapy, 2019, 13: 103-117.
[3]	XIE H Q, LI X T, CHEN Y J, et al. Ethanolic extract of Cordyceps cicadae exerts antitumor effect on human gastric cancer SGC-7901 cells by inducing apoptosis, cell cycle arrest and endoplasmic Reticulum stress[J]. Journal of Ethnopharmacology, 2019, 231: 230-240. DOI URL
[4]	ZHU Y L, YU X F, GE Q, et al. Antioxidant and anti-aging activities of polysaccharides from Cordyceps cicadae[J]. International Journal of Biological Macromolecules, 2020, 157: 394-400. DOI URL
[5]	SHARMA S K, GAUTAM N. Chemical composition and antioxidant and antibacterial activities of cultured mycelia of four clavicipitaceous mushrooms (ascomycetes) from the Indian Himalayas[J]. International Journal of Medicinal Mushrooms, 2017, 19(1): 45-54. DOI URL
[6]	ZHANG Y, WU Y T, ZHENG W, et al. The antibacterial activity and antibacterial mechanism of a polysaccharide from Cordyceps cicadae[J]. Journal of Functional Foods, 2017, 38: 273-279. DOI URL
[7]	WENG S C, CHOU C J, LIN L C, et al. Immunomodulatory functions of extracts from the Chinese medicinal fungus Cordyceps cicadae[J]. Journal of Ethnopharmacology, 2002, 83(1/2): 79-85. DOI URL
[8]	ZENG W B, YU H, GE F, et al. Distribution of nucleosides in populations of Cordyceps cicadae[J]. Molecules (Basel, Switzerland), 2014, 19(5): 6123-6141. DOI URL
[9]	LIU K B, WANG F, LIU G J, et al. Effect of environmental conditions on synnema formation and nucleoside production in Cicada flower, Isaria cicadae(ascomycetes)[J]. International Journal of Medicinal Mushrooms, 2019, 21(1): 59-69. DOI URL
[10]	ZHU R, ZHENG R, DENG Y Y, et al. Ergosterol peroxide from Cordyceps cicadae ameliorates TGF-β1-induced activation of kidney fibroblasts[J]. Phytomedicine, 2014, 21(3): 372-378. DOI URL
[11]	HUR H. Chemical ingredients of Cordyceps militaris[J]. Mycobiology, 2008, 36(4): 233. DOI URL
[12]	BROWN K M, ARTHUR J R. Selenium, selenoproteins and human health: a review[J]. Public Health Nutrition, 2001, 4(2B): 593-599. DOI URL
[13]	RAYMAN M P. Selenoproteins and human health: Insights from epidemiological data[J]. Biochimica et Biophysica Acta (BBA): General Subjects, 2009, 1790(11): 1533-1540.
[14]	HARIHARAN S, DHARMARAJ S. Selenium and selenoproteins: it’s role in regulation of inflammation[J]. Inflammopharmacology, 2020, 28(3): 667-695. DOI URL
[15]	RAYMAN M P. Selenium and human health[J]. The Lancet, 2012, 379(9822): 1256-1268. DOI URL
[16]	KURŠVIETIENĖ L, MONGIRDIENĖ A, BERNATONIENĖ J, et al. Selenium anticancer properties and impact on cellular redox status[J]. Antioxidants, 2020, 9(1): 80. DOI URL
[17]	ALLAN C B, LACOURCIERE G M, STADTMAN T C. Responsiveness of selenoproteins to dietary selenium[J]. Annual Review of Nutrition, 1999, 19(1): 1-16. DOI URL
[18]	LIPINSKI B. Sodium selenite as an anticancer agent[J]. Anti-Cancer Agents in Medicinal Chemistry, 2017, 17(5): 658-661. DOI URL
[19]	LOSCALZO J. Keshan disease, selenium deficiency, and the selenoproteome[J]. The New England Journal of Medicine, 2014, 370(18): 1756-1760. DOI URL
[20]	HARTIKAINEN H. Biogeochemistry of selenium and its impact on food chain quality and human health[J]. Journal of Trace Elements in Medicine and Biology, 2005, 18(4): 309-318. DOI URL
[21]	LI C S. Selenium deficiency and endemic heart failure in China: a case study of biogeochemistry for human health[J]. Ambio, 2007, 36(1): 90-93. DOI URL
[22]	DINH Q T, CUI Z W, HUANG J, et al. Selenium distribution in the Chinese environment and its relationship with human health: a review[J]. Environment International, 2018, 112: 294-309. DOI URL
[23]	DUNTAS L H, BENVENGA S. Selenium: an element for life[J]. Endocrine, 2015, 48(3): 756-775. DOI URL
[24]	COMBS G F. Selenium in global food systems[J]. The British Journal of Nutrition, 2001, 85(5): 517-547. DOI URL
[25]	KIELISZEK M. Selenium-fascinating microelement, properties and sources in food[J]. Molecules, 2019, 24(7): 1298. DOI URL
[26]	WIESNER-REINHOLD M, SCHREINER M, BALDERMANN S, et al. Mechanisms of selenium enrichment and measurement in brassicaceous vegetables, and their application to human health[J]. Frontiers in Plant Science, 2017, 8: 1365. DOI URL
[27]	ZHAO L, ZHAO G H, ZHAO Z D, et al. Selenium distribution in a Se-enriched mushroom species of the genus Ganoderma[J]. Journal of Agricultural and Food Chemistry, 2004, 52(12): 3954-3959. DOI URL
[28]	FALANDYSZ J. Selenium in edible mushrooms[J]. Journal of Environmental Science and Health: Part C, 2008, 26(3): 256-299.
[29]	柴新义, 倪瓒鹏, 于士军, 等. 黑木耳菌丝体液体发酵富硒条件优化及其多糖抗氧化活性[J]. 浙江农业学报, 2017, 29(11): 1903-1911.
	CHAI X Y, NI Z P, YU S J, et al. Optimization of Se-rich liquid fermentation conditions and antioxidant activity of polysaccharide from Auricularia auricula mycelia[J]. Acta Agriculturae Zhejiangensis, 2017, 29(11): 1903-1911.(in Chinese with English abstract)
[30]	于田田, 钱和. 生物富硒对蛹虫草菌丝体化学成分的影响[J]. 食品科技, 2006, 31(1): 133-135.
	YU T T, QIAN H. Effect of selenium on the chemical components of Cordyceps militaris[J]. Food Science and Technology, 2006, 31(1): 133-135.(in Chinese with English abstract)
[31]	HU T, LIANG Y, ZHAO G S, et al. Selenium biofortification and antioxidant activity in Cordyceps militaris supplied with selenate, selenite, or selenomethionine[J]. Biological Trace Element Research, 2019, 187(2): 553-561. DOI URL
[32]	王新风, 杨芳, 刘圣师, 等. 富硒平菇蛋白测定与氨基酸成分分析[J]. 食品科学, 2008, 29(12): 610-613.
	WANG X F, YANG F, LIU S S, et al. Determination of soluble protein and analysis of amino acid compositions of selenium(Se)-accumulated Pleurotus ostreatus[J]. Food Science, 2008, 29(12): 610-613.(in Chinese with English abstract)
[33]	陈秀明, 张子皓, 李双, 等. 杜马斯燃烧定氮法在食品检测中的应用[J]. 理化检验:化学分册, 2015, 51(11): 1617-1618.
	CHEN X M, ZHANG Z H, LI S, et al. Application of Dumas combustion nitrogen determination method in food detection[J]. Physical Testing and Chemical Analysis: Part B Chemical Analysis), 2015, 51(11): 1617-1618.(in Chinese)
[34]	尚红霞, 梁晨, 易娟. 高效液相色谱法同时测定虫草素和腺苷[J]. 分析科学学报, 2011, 27(6): 776-778.
	SHANG H X, LIANG C, YI J. Simultaneous dertermation of cordycepin and adenosine by high performance liquid chromatography[J]. Journal of Analytical Science, 2011, 27(6): 776-778. (in Chinese with English abstract)
[35]	李颜, 史薇娜, 唐庆九, 等. 比色法测定冬虫夏草和蛹虫草中多糖和甘露醇的含量[J]. 食用菌学报, 2007, 14(3): 53-57.
	LI Y, SHI W N, TANG Q J, et al. Determination of polysaccharides and mannitol in Cordyceps sinensis and Cordyceps militaris using colorimetric methods[J]. Acta Edulis Fungi, 2007, 14(3): 53-57.(in Chinese with English abstract)
[36]	刘文芳, 肖美. 石墨炉原子吸收分光光度法测定硒[J]. 江西化工, 2007(1): 94-95.
	LIU W F, XIAO M. Determination of selenium by graphite furnace atomic absorption spectrophotometry[J]. Jiangxi Chemical Industry, 2007(1): 94-95.(in Chinese)
[37]	SCHISLER L C, CHANG S T, QUIMIO T H. Tropical mushrooms: biological nature and cultivation methods[J]. Mycologia, 1984, 76(2): 383. DOI URL
[38]	朱圣陶, 吴坤. 蛋白质营养价值评价: 氨基酸比值系数法[J]. 营养学报, 1988, 10(2): 187-190.
	ZHU S T, WU K. Nutritional evaluation of protein: ratio coefficient of amino acid[J]. Acta Nutrimenta Sinica, 1988, 10(2): 187-190.(in Chinese with English abstract)
[39]	Food Policy and Nutrition Div, FAO, ESN. Amino-acid content of foods and biological data on proteins[J]. Annals of Internal Medicine, 1970, 73(6): 1059.
[40]	刘文玲, 冮洁, 解彬, 等. 富硒香菇硒分布和蛋白质营养价值的评价[J]. 食品与发酵工业, 2019, 45(2): 101-106.
	LIU W L, GANG J, XIE B, et al. The distribution of selenium in Lentinus edodes and evaluation of its nutrient value of protein[J]. Food and Fermentation Industries, 2019, 45(2): 101-106.(in Chinese with English abstract)
[41]	DONG J Z, LEI C, AI X R, et al. Selenium enrichment on Cordyceps militaris link and analysis on its main active components[J]. Applied Biochemistry and Biotechnology, 2012, 166(5): 1215-1224. DOI URL
[42]	杨月欣, 王光亚, 潘兴昌. 中国食物成分表[M]. 北京: 北京大学医学出版社, 2002.
[43]	HAWKINS R, VIÑA J. How glutamate is managed by the blood: brain barrier[J]. Biology, 2016, 5(4): 37. DOI URL
[44]	SLOMINSKI A, ZMIJEWSKI M A, PAWELEK J. L-tyrosine and L-dihydroxyphenylalanine as hormone-like regulators of melanocyte functions[J]. Pigment Cell & Melanoma Research, 2012, 25(1): 14-27.
[45]	FERNSTROM J D, FERNSTROM M H. Tyrosine, phenylalanine, and catecholamine synjournal and function in the brain[J]. The Journal of Nutrition, 2007, 137(6 Suppl 1): 1539S-1547S. DOI URL
[46]	DONG W J, HU R S, CHU Z, et al. Effect of different drying techniques on bioactive components, fatty acid composition, and volatile profile of robusta coffee beans[J]. Food Chemistry, 2017, 234: 121-130. DOI URL
[47]	孟泽彬, 文庭池, 姜金仲. 富硒虫草的研究进展[J]. 微生物学通报, 2014, 41(11): 2339-2348.
	MENG Z B, WEN T C, JIANG J Z. Review on selenium-enriched Cordyceps[J]. Microbiology China, 2014, 41(11): 2339-2348.(in Chinese with English abstract)

成分含量 Component content	对照组 Control	Na₂SeO₃/(mg·kg^-1)
成分含量 Component content	对照组 Control	50	75	100
硒含量Selenium content/(mg·kg^-1)	0.18±0.03 d	83.62±3.74 c	115.61±4.89 b	142.22±5.41 a
腺苷Adenosine/(mg·g^-1)	1.18±0.05 b	1.31±0.06 a	1.42±0.07 a	1.41±0.04 a
甘露醇Mannitol/(mg·g^-1)	53.15±5.25 c	52.11±5.30 c	66.56±5.87 b	78.63±4.97 a
脂肪Fat/%	3.24±0.49 c	3.52±0.51 c	5.56±0.83 b	6.68±0.43 a
多糖Polysaccharide/%	15.16±1.94 a	15.77±3.08 a	11.17±0.16 b	8.93±0.69 b
蛋白质Protein/%	29.78±0.54 d	34.47±0.68 b	31.57±0.29 c	35.92±0.75 a

成分含量 Component content	对照组 Control	Na₂SeO₃/(mg·kg^-1)
成分含量 Component content	对照组 Control	50	75	100
硒含量Selenium content/(mg·kg^-1)	0.18±0.03 d	83.62±3.74 c	115.61±4.89 b	142.22±5.41 a
腺苷Adenosine/(mg·g^-1)	1.18±0.05 b	1.31±0.06 a	1.42±0.07 a	1.41±0.04 a
甘露醇Mannitol/(mg·g^-1)	53.15±5.25 c	52.11±5.30 c	66.56±5.87 b	78.63±4.97 a
脂肪Fat/%	3.24±0.49 c	3.52±0.51 c	5.56±0.83 b	6.68±0.43 a
多糖Polysaccharide/%	15.16±1.94 a	15.77±3.08 a	11.17±0.16 b	8.93±0.69 b
蛋白质Protein/%	29.78±0.54 d	34.47±0.68 b	31.57±0.29 c	35.92±0.75 a

氨基酸种类 Amino type	对照组 Control	Na₂SeO₃/(mg·kg^-1)
氨基酸种类 Amino type	对照组 Control	50	75	100
天冬氨酸Asp	1.12	1.09	1.74	1.75
苏氨酸Thr^*	1.35	1.46	1.24	1.38
丝氨酸Ser	1.10	1.22	1.08	1.15
谷氨酸Glu	3.37	2.74	2.23	2.38
甘氨酸Gly	0.89	1.05	1.04	1.14
丙氨酸Ala	1.42	1.60	1.54	1.58
半胱氨酸Cys^*	0.46	0.51	0.34	0.43
缬氨酸Val^*	0.98	0.95	0.95	0.96
蛋氨酸Met^*	1.47	1.82	1.36	1.79
异亮氨酸Ile^*	0.75	0.75	0.78	0.79
亮氨酸Leu^*	1.31	1.25	1.32	1.33
酪氨酸Tyr	1.96	1.80	1.74	1.97
苯丙氨酸Phe^*	0.96	0.94	0.96	1.01
赖氨酸Lys^*	1.01	0.94	0.92	1.03
组氨酸His	0.46	0.44	0.45	0.46
精氨酸Arg	1.14	1.22	1.16	1.30
脯氨酸Pro	1.77	1.41	1.46	1.51
必需氨基酸EAA	7.81	8.11	7.52	8.29
总氨基酸TAA	21.49	21.18	20.29	21.96
EAA/TAA	36.35	38.27	37.08	37.76

氨基酸种类 Amino type	对照组 Control	Na₂SeO₃/(mg·kg^-1)
氨基酸种类 Amino type	对照组 Control	50	75	100
天冬氨酸Asp	1.12	1.09	1.74	1.75
苏氨酸Thr^*	1.35	1.46	1.24	1.38
丝氨酸Ser	1.10	1.22	1.08	1.15
谷氨酸Glu	3.37	2.74	2.23	2.38
甘氨酸Gly	0.89	1.05	1.04	1.14
丙氨酸Ala	1.42	1.60	1.54	1.58
半胱氨酸Cys^*	0.46	0.51	0.34	0.43
缬氨酸Val^*	0.98	0.95	0.95	0.96
蛋氨酸Met^*	1.47	1.82	1.36	1.79
异亮氨酸Ile^*	0.75	0.75	0.78	0.79
亮氨酸Leu^*	1.31	1.25	1.32	1.33
酪氨酸Tyr	1.96	1.80	1.74	1.97
苯丙氨酸Phe^*	0.96	0.94	0.96	1.01
赖氨酸Lys^*	1.01	0.94	0.92	1.03
组氨酸His	0.46	0.44	0.45	0.46
精氨酸Arg	1.14	1.22	1.16	1.30
脯氨酸Pro	1.77	1.41	1.46	1.51
必需氨基酸EAA	7.81	8.11	7.52	8.29
总氨基酸TAA	21.49	21.18	20.29	21.96
EAA/TAA	36.35	38.27	37.08	37.76

氨基酸 Amino acid/(mg·g^-1)	鸡蛋模式 Egg mode	FAO/WHO模式 FAO/WHO mode	对照组 Control	Na₂SeO₃/(mg·kg^-1)
氨基酸 Amino acid/(mg·g^-1)	鸡蛋模式 Egg mode	FAO/WHO模式 FAO/WHO mode	对照组 Control	50	75	100
异亮氨酸Ile	54	40	25.16	21.77	24.77	22.06
亮氨酸Leu	86	70	43.95	36.34	41.83	37.14
赖氨酸Lys	70	55	33.87	27.20	29.12	28.61
蛋氨酸+半胱氨酸Met+Cys	57	35	64.78	67.59	53.90	67.75
苯丙氨酸+酪氨酸Phe+Tyr	93	60	98.10	79.60	85.46	83.19
苏氨酸Thr	47	40	45.22	42.28	39.14	38.33
缬氨酸Val	66	50	32.74	27.54	30.01	26.80
总必需氨基酸TEAA	473	350	343.82	302.33	304.22	297.88

Effect of selenium on nutritional and functional components of synnemata of Isaria cicadae

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处理 Treatment		必需氨基酸指数 EAAI	生物价 BV	营养指数 NI
对照组Control		73.01	67.88	21.74
Na₂SeO₃/	50	65.33	59.51	22.52
(mg·kg^-1)	75	64.47	58.57	20.35
	100	63.55	57.57	22.83

氨基酸 Amino acid	对照组 Control		Na₂SeO₃/(mg·kg^-1)
	对照组 Control		50		75		100
	RAA	RCAA	RAA	RCAA	RAA	RCAA	RAA	RCAA
异亮氨酸Ile	0.63	0.62	0.54	0.59	0.62	0.69	0.55	0.62
亮氨酸Leu	0.63	0.62	0.52	0.57	0.60	0.67	0.53	0.59
赖氨酸Lys	0.62	0.60	0.49	0.54	0.53	0.59	0.52	0.58
蛋氨酸+半胱氨酸Met+Cys	1.85	1.81	1.93	2.10	1.54	1.71	1.76	1.98
苯丙氨酸+酪氨酸Phe+Tyr	1.63	1.60	1.33	1.45	1.42	1.59	1.39	1.55
苏氨酸Thr	1.13	1.11	1.06	1.15	0.98	1.09	0.96	1.07
缬氨酸Val	0.65	0.64	0.55	0.60	0.60	0.67	0.54	0.60
均数Average value	1.02	1.00	0.92	1.00	0.90	1.00	0.89	1.00
SRCAA	48.09		39.89		52.60		43.58

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[5]	YANG Haitao, ZHANG Jing, MA Hongyan, YANG Yan, DING Dongxia, LYU Jian, XIE Jianming. Effects of different selenium sources on quality and nutrient absorption of pakchoi [J]. Acta Agriculturae Zhejiangensis, 2022, 34(10): 2199-2208.
[6]	LIANG Le, LIU Juan, LI Xiaomei, LIAO Jichao, LI Huanxiu, TANG Yi. Effects of intercropping with different genotypes of cherry tomato on fruit quality and selenium content [J]. Acta Agriculturae Zhejiangensis, 2021, 33(10): 1870-1878.
[7]	HUANG Kewen, LI Keqiang, LIU Ji, SUI Liyun, LIU Lei, WANG Ting, ZHENG Yangxia, LIN Lijin, LIAO Ming'an. Effects of different rootstock combinations on physiological characteristics and selenium accumulation of Nasturtium officinale cuttings [J]. , 2020, 32(3): 447-454.
[8]	LI Le, TIAN Minjiao, GAO Yanming, LI Jianshe. Effect of selenium fertilizer on growth and mineral element accumulation of tomato in substrate culture [J]. , 2020, 32(2): 253-261.
[9]	LIU Kang, HU Chengxiao, CAI Miaomiao, GAO Lin, ZHANG Mengjiao, BA Lei, YANG Dandan, WANG Xu, ZHAO Xiaohu. Effect of selenium on spectral characteristics of chromium interaction with bovine serum albumin [J]. , 2020, 32(1): 149-159.
[10]	XING Chaogang, YUAN Jingqun, LI Shimin. Determination of trace selenium in agricultural products with microwave digestion-dynamic reaction cell inductively coupled plasma mass spectrometry [J]. , 2018, 30(8): 1414-1419.
[11]	JIN Erhui, ZHOU Jinxing, REN Man, Hu Qianqian, JIN Guangming, LI Shenghe. Combined effects of yeast selenium and boron on structure of immune organs and immune function of broilers [J]. , 2017, 29(11): 1783-1795.
[12]	CHAI Xinyi, NI Zanpeng, YU Shijun, ZHANG Weiwei, YIN Peifeng. Optimization of Se-rich liquid fermentation conditions and antioxidant activity of polysaccharide from Auricularia auricula mycelia [J]. , 2017, 29(11): 1903-1911.
[13]	MING Jia-jia, HU Cheng-xiao, ZHAO Xiao-hu, ZHENG Ya-wei, LIU Xin-wei, ZHAO Ze, ZHAO Yuan-yuan, JIA Wei. Content and migration characteristic of mineral element in rape with application of selenium [J]. , 2016, 28(9): 1564-1571.
[14]	WU Yi-fei, SUN Hong, LI Yuan-cheng, WANG Xin, LIU Yong, YAO Xiao-hong, TANG Jiang-wu. Effects of microbial solid-state fermentation on nutritional value of feeds [J]. , 2016, 28(12): 2014-2020.
[15]	YUAN Ju-hong, HU Mian-hao. Elements distribution and their correlation analysis in Coleus blumei Benth under selenium treatments [J]. , 2016, 28(12): 2098-2108.