Abstract:

In this study, researchers conducted a research on the strains of Sanghuang planted by Yongfu Plantation Cooperation of Jinzhai Country in Dabie Mountain Area and identified the strain as Sanghuang vaninii by cloning and sequencing the ITS region of its 18S rDNA gene using modern molecular biology techniques. Screening of the basal medium for JS-1 showed that PDA medium was the most suitable for the growth of JS-1. The growth characteristics of JS-1 strain were determined, and the results showed that the mycelium could grow in the temperature range of 5-35 ℃, and the optimum growth temperature was 28 ℃. The mycelium could grow in the pH range of 4-10, while the pH value of 7 was the optimal for the growth of mycelium. By taking the mycelium growth as an indicator to screen the carbon and nitrogen sources, it was found that the medium had the fastest and dense mycelium growth when glucose was used as the carbon source. Thus, glucose was considered the most suitable carbon source. When potato juice was used as the nitrogen source of the medium, despite of the slightly slower growth rate than peptone and corn flour, the colony growth was the most luxuriant, with the earliest appearance of yellow-brown cork-like ring. Thus, potato juice was considered the most suitable nitrogen source for its growth.On the basis of glucose as carbon source and potato extract as nitrogen source, L₉ (3 ⁴) orthogonal test showed that the optimal growth conditions of JS-1 strain were glucose 20 g·L^-1 and potato 100 g·L^-1, pH 8 and culture temperature 30 ℃. The mycelium lethal temperature was 45 ℃.

Key words: Sanghuang, species identification, medium, growth conditions