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    Crop Science
    Effects of different sulfate soaking agents on seed germination and cadmium uptake of rice seedlings
    DONG Aiqin, LI Jianguo, YANG Tao, CHEN Yuanhua, XU Changxu, WAN Hui, PENG Zhiping, XIE Jie
    2021, 33(12):  2213-2223.  DOI: 10.3969/j.issn.1004-1524.2021.12.01
    Abstract ( 1150 )   HTML ( 1736750 )   PDF (2906KB) ( 1255 )  

    To study the effects of sulfate soaking on seed germination and cadmium (Cd) uptake of rice seedlings, rice variety Wufengyou 286 was selected as the test material, and FeSO4, ZnSO4, MnSO4 solutions with various concentrations were used as seed soaking agents in the sprouting and hydroponic experiment. The radicle length, plumule length, germination rate and contents of Cd, Mg, B, P, K in rice seedlings were determined. It was shown that the seed soaking with FeSO4 or MnSO4 solution could increase the germination rate of rice, promote the growth of radicle and plumule, and effectively reduce Cd content of rice seedlings. However, the seed soaking with ZnSO4 solution inhibited the growth of radicle and plumule, and increased Cd content of rice seedlings. The seed soaking with FeSO4 or MnSO4solution had no obvious inhibition on the uptake of Mg, B, P, K, and the seed soaking with FeSO4 solution even could promote B uptake of rice seedlings. In general, the seed soaking with FeSO4or MnSO4 solution had application potential for reducing Cd uptake of rice, and was worthy for further research.

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    Changes of nutritional quality and starch properties of different types of sweet potato roots during storage
    ZHAO Lin, YE Xiafang, DONG Wei, SHI Jiang, LUO Letan, LUO Guoquan
    2021, 33(12):  2224-2233.  DOI: 10.3969/j.issn.1004-1524.2021.12.02
    Abstract ( 856 )   HTML ( 98305 )   PDF (2048KB) ( 1242 )  

    The quality of sweet potato changes during storage. In order to study of the changes of sweet potato quality and starch characteristics during different storage periods, changes in nutritional quality and starch properties of starchy sweet potato ZH1042, fresh food sweet potato ZZ1-358 and control variety Xinxiang during storage were analyzed. The results showed that: changes in quality and starch characteristics of different types of sweet potato varieties were different with time extension. The starchy sweet potato had the highest water loss rate and decay rate during storage, while Xinxiang had the lowest decay rate during the entire storage period. The total amylase activity and β-amylase activity of the three sweet potato varieties (lines) showed a trend of first rising, then falling and then rising with the extension of storage time, and differences were significant. The starch content of ZH1042 decreased first and then increased. Soluble sugar content and protein content generally increased first and then decreased. Content of soluble sugar did not change significantly before and after the storage period, while protein content decreased significantly after 100 days of storage. Starch content of ZZ1-358 first decreased and then increased, soluble sugar content decreased, while the protein content first increased, then decreased and then increased. Starch content of Xinxiang increased significantly after storage for 100 days, soluble sugar content decreased with extension of storage time, and protein content showed a trend of first increase, then decrease and then increase with extension of storage time, the difference was significant. This showed that level of starch content in sweet potato directly affected content of soluble sugar and protein in sweet potato. During the storage period of 70-100 d, the starch extracted from different types of sweet potato materials had the highest purity. The highest viscosity value, final viscosity value, recovery value and hot pulp viscosity value would decrease significantly when the sweet potato was stored for 100 d. The starch content in the sweet potato could directly affecting soluble sugar and protein content, storage would change X-ray diffraction intensity of sweet potato starch but not its crystal structure. The storage time of most varieties could not exceed 130 d.

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    Identification and growth characteristics of Sanghuang in Jinzhai County
    CHENG Andong, WANG Benqin
    2021, 33(12):  2234-2244.  DOI: 10.3969/j.issn.1004-1524.2021.12.03
    Abstract ( 745 )   HTML ( 196607 )   PDF (21325KB) ( 1215 )  

    In this study, researchers conducted a research on the strains of Sanghuang planted by Yongfu Plantation Cooperation of Jinzhai Country in Dabie Mountain Area and identified the strain as Sanghuang vaninii by cloning and sequencing the ITS region of its 18S rDNA gene using modern molecular biology techniques. Screening of the basal medium for JS-1 showed that PDA medium was the most suitable for the growth of JS-1. The growth characteristics of JS-1 strain were determined, and the results showed that the mycelium could grow in the temperature range of 5-35 ℃, and the optimum growth temperature was 28 ℃. The mycelium could grow in the pH range of 4-10, while the pH value of 7 was the optimal for the growth of mycelium. By taking the mycelium growth as an indicator to screen the carbon and nitrogen sources, it was found that the medium had the fastest and dense mycelium growth when glucose was used as the carbon source. Thus, glucose was considered the most suitable carbon source. When potato juice was used as the nitrogen source of the medium, despite of the slightly slower growth rate than peptone and corn flour, the colony growth was the most luxuriant, with the earliest appearance of yellow-brown cork-like ring. Thus, potato juice was considered the most suitable nitrogen source for its growth.On the basis of glucose as carbon source and potato extract as nitrogen source, L9 (3 4) orthogonal test showed that the optimal growth conditions of JS-1 strain were glucose 20 g·L-1 and potato 100 g·L-1, pH 8 and culture temperature 30 ℃. The mycelium lethal temperature was 45 ℃.

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    Effect of selenium on nutritional and functional components of synnemata of Isaria cicadae
    YU Shijun, HE Lingyan, CHENG Ming, XU Xin, XU Xinyi, CHAI Xinyi, WANG Weikun
    2021, 33(12):  2245-2253.  DOI: 10.3969/j.issn.1004-1524.2021.12.04
    Abstract ( 684 )   HTML ( 11534338 )   PDF (964KB) ( 1070 )  

    The effect of selenium on the the nutrition and functional components in the synnemata of Isaria cicadae was explored by the selenium-enriched culture of synnemata of I. cicadae using sodium selenite as the source of selenium. And the nutritional value of the protein of synnemata of I. cicadae was evaluated. The results showed that the selenium amount of synnemata increased with the increase of sodium selenite in the culture media. When the concentration of sodium selenite was 100 mg·kg -1 in the medium, the selenium content in synnemata of I. cicadae reached 142.22 mg·kg -1. The contents of protein, fat, adenosine and mannitol in the selenium-enriched synnemata of I. cicadae were significantly higher than those of the control group. The amino acid ratio coefficient score (SRCAA) of protein of synnemata of I. cicadae, cultured in the media with 75 mg·kg -1 sodium selenite, was 52.60, which is higher than the control group. Cluster analysis indicated that, in general, the effect of sodium selenite on the nutritional and functional components of synnemata of I. cicadae were increased with the increase of sodium selenite in the culture media. It can be concluded that when sodium selenite was 75 mg·kg -1 in the media, it could not only increase the contents of protein, fat, adenosine and mannitol in synnemata of I. cicadae, but also increase its protein nutritional value.

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    Animal Science
    Establishment of in vitro culture system of pig spermatogonial stem cells
    ZHANG Mao, ZHAO Xin, CAI Gengyuan, YANG Huaqiang
    2021, 33(12):  2254-2263.  DOI: 10.3969/j.issn.1004-1524.2021.12.05
    Abstract ( 864 )   HTML ( 902 )   PDF (7355KB) ( 1050 )  

    Spermatogonial stem cells (SSCs) are the guarantee of spermatogenesis and fertility in male mammals. In vitro culture of spermatogonial stem cells not only provides materials for the study of spermatogenesis, but also helps to develop new methods of livestock conservation and animal genetic modification. In order to find out the better culture system of porcine spermatogonial stem cells in vitro, two step enzymatic method of collagenase Ⅳ-trypsin was used to digest the testis of 3-7 day Yorkshire piglets to obtain single cell suspension. And the spermatogonial stem cells were purified using differential adherent with different time programs. In addition, different cytokines were added to study the best culture system for the proliferation of spermatogonial stem cells. The results showed that the highest percentage of UCHL-1 positive germ cells was 18.59%±0.94%, and the result of cytokine addition showed that spermatogonial stem cells added with 20 ng·mL -1 GDNF, 10 ng·mL-1 IGF and 20 ng·mL-1 bFGF had the best effect of proliferation. Spermatogonial stem cells were cultured in DMEM/F12 with 1% FBS and the cytokines described above, taking Sertoli cells as feeder laye. After 15 days, a large number of spermatogonial stem cell colonies were observed. The proliferation of spermatogonial stem cells was confirmed by immunofluorescence, AKP staining and fluorescent quantitative PCR detcetion. In brief, we initially established the culture system of porcine spermatogonial stem cells in vitro, through which a large number of spermatogonial stem cells can be cultured in vitro, laying the foundation for subsequent research on spermatogonial stem cells.

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    Study on depositions of mineral elements and expression levels of key genes in different tissues of Liancheng white ducks
    BAI Hao, LI Xiaofan, ZHONG Li, SONG Qianqian, JIANG Yong, ZHANG Yang, WANG Zhixiu, XU Qi, CHANG Guobin, CHEN Guohong
    2021, 33(12):  2264-2274.  DOI: 10.3969/j.issn.1004-1524.2021.12.06
    Abstract ( 653 )   HTML ( 1441791 )   PDF (1097KB) ( 1229 )  

    This study was conducted to investigate the depositions of several mineral elements and the mRNA expression levels of mineral-related genes in different tissues of Liancheng white duck. The contents of Mg, K, Fe, Zn and Se in breast muscle, thigh muscle, liver, skin and tibia at 0, 21, 35, 49 and 63 days of age, respectively, were measured by atomic fluorescence spectrophotometer. The mRNA expression levels of mineral-related genes were detected by qRT-PCR. The results revealed that the depositions of Mg and K were basically the same in all tissues, showing a significant positive correlation (P<0.05). In view of the breast muscle, thigh muscle and liver, the contents of almost all the mineral elements reached the peak value (P<0.05) at the age of 49-63 days. The expression levels of Mg-related genes TRPM6 and TRPM7 were the highest at the age of 21 days (P<0.05); the expression levels of K-related genes ATP1A1 and ATP1B1 were the highest at birth (P<0.05); the expression of Fe-related gene FTH1 showed a significant decrease and then a significant increase with the change of age, which had the lowest expression at the age of 35 days (P<0.05); the expression levels of Zn-related genes ATP6 and ATP8 were the highest at 0 and 21 days, respectively (P<0.05); the expression levels of Se-related genes GPX1 and GPX4 were the highest at birth (P<0.05). In addition, there were negative correlations between the expression level of genes and the deposition of mineral elements in liver tissue but not significantly (P>0.05). Taken together, Mg and K could be used as joint indicators for the precise breeding of high quality strain of Liancheng white duck; 49-63 days can be used as the reference of the best marketing age.

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    Identification and pathological observation of Aeromonas veronii from hybrid sturgeon (Acipenser baerii×Acipenser schrenckii♂)
    YANG Chengnian, LI Fang, ZHU Chengke, TANG Zhengxian, YI Zilin, HAN Lulu, YANG Longjiang, PENG Xiaoqian, HE Die, LI Yang, REN Chaoying, LYU Guangjun
    2021, 33(12):  2275-2285.  DOI: 10.3969/j.issn.1004-1524.2021.12.07
    Abstract ( 790 )   HTML ( 11534335 )   PDF (4632KB) ( 1251 )  

    In order to find out the reason that caused death of hybrid sturgeon in Pengshui, Chongqing, a dominant strain named CQST-2 was obtained from liver of diseased hybrid sturgeon. Pathogen morphological observation, challenge test, physical and chemical characteristics of isolated bacteria, sequence analysis of 16S rRNA,gyrB, rpoB and cpn60 genes, drug test and pathological were carried out and investigated. The result indicated that the isolated strain was a gram-negative bacterium and challenge test symptoms infected with Aeromonas veronii were similar to the natural symptoms. Phylogenetic trees constructed by 16S rRNA, gyrB, rpoB and cpn60 gene sequences were the same as A. veronii, with similarity of 99.7%, 99.9%, 99.4% and 99.8%. The challenge test showed that the value of LD50 was 2.0×105 CFU·g-1 fish weight by intraperitoneal injection at (22±1)℃. Five virulent genes, including ompA, act, aer, hlyA and alt genes were screened by PCR assay. Pathological observation showed that the structure of liver cells was fuzzy, hepatic sinuses were dilated and congested. Most of intestinal villi were necrotic and exfoliated, and muscle layer was congested.Gills of infected fish were curved and epithelial cells were hyperplasia. Antibiotic resistances showed that the strain was sensitive to 18 drugs, including ofloxacin, polymyxinB, ciprofloxacin, enrofloxacin, and resistance to 9 drugs, including medications, guitamycin, penicillin, et al.This study provided that disease of hybrid sturgeon caused by A. veronii, which had strong pathogenicity and could cause multiple tissue lesions of hybrid sturgeon.

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    Isolation and biological characteristics of a pathogenic ST-251 Aeromonas hydrophila from Procypris Rabaudi (rock carp)
    CHEN Mengzhu, KANG Zhenya, GUO Xianghui, GENG Yi, BAI Minghuan, OUYANG Ping, CHEN Defang, HUANG Xiaoli, LAI Weimin
    2021, 33(12):  2286-2294.  DOI: 10.3969/j.issn.1004-1524.2021.12.08
    Abstract ( 737 )   HTML ( 20482 )   PDF (3232KB) ( 1256 )  

    To explore the causes of epidemic diseases characterized by body surface bleeding in a Procypris rabaudi farm in Yibin, the pathogen and its biological characteristics were identified by physiological and biochemical characteristics,research on the pathogenesis,16S rDNA, multilocus sequence typing (MLST) and Kirby-Bauer (K-B). A pathogenic bacterium (YYL) was isolated from liver and kidney of diseased fish, and identified as Aeromonas hydrophila.When the water temperature was (24±1) ℃, its median lethal dose (LD50) was 5.8 × 10 4 CFU·g-1 for rock carp weight of (12.6±0.8) g. Drug sensitivity test showed that the YYL was sensitive to many drugs, such as ceftazidime, gentamicin, doxycycline, enrofloxacin, florfenicol, but resistant to imipenem, roxithromycin, compound sulfamethoxazole, clindamycin. The isolated A. hydrophila belonged to ST-251 type by multilocus sequence typing (MLST), and carried virulence genes aerA, hlyA, aphA, act, ast. In this study, ST-251 A. hydrophila was isolated from the diseased rock carp characterized by body surface hemorrhage. This strain carried a variety of virulence factors and had strong pathogenicity. Therefore, it was necessary to strengthen the prevention and control of this pathogen infection in the cultivation of rock carp.

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    Optimization of Chinese medicine plant extract compounds with orthogonal design
    TAO Xin, WU Wanyun, SUN Yuqing, MEN Xiaoming, XU Ziwei
    2021, 33(12):  2295-2303.  DOI: 10.3969/j.issn.1004-1524.2021.12.09
    Abstract ( 561 )   HTML ( 24575 )   PDF (896KB) ( 1134 )  

    To prepare Chinese medicine plant extracts replacing feed antibiotics. Firstly, Grana Pericarpium, Cinnamomi Ramulus, Magnoliae officinalis cortex, Artemisiae annuae herba, Spatholobi caulis were sieved from 19 Chinese medicine plants as materials of extract compounds by examining antibacterial rate, inhibitory rateanti-inflammatory rate, and antioxidant IC50 value as evaluating index. Secondly, proportions of five materials were optimized by a L16 (4 5) orthogonal experiment,by range analysis of results, three extract compounds with the strongest antibacterial (Fab), anti-inflammatory (Fai) and antioxidant (Fao) activities were obtained. These material ratios of Magnoliae officinalis cortex, Spatholobi caulis, Artemisiae annuae herba, Cinnamomi Ramulus and Grana Pericarpium were 7∶13∶6∶9∶1 in Fab, 7∶13∶4∶1∶1 in Fai, and 3∶13∶4∶1∶7 in Fao, respectively. Finally, the verifying results showed that the minimum inhibitory concentration of Fab was 125 mg·mL-1, the anti-inflammatory IC50 value of Fai was 0.144 mg·mL-1, and the antioxidant IC50 value of Fao was 0.222 mg·mL-1. In summary, the present study obtained the best three Chinese medicine extract compounds with stronger antibacterial, anti-inflammatory and antioxidant effects, respectively.

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    Horticultural Science
    Construction of DNA fingerprint for 48 broccoli cultivars
    LIN Mengjie, WEN Huiping, XIAO Jianzhong, ZHENG Qiang
    2021, 33(12):  2304-2312.  DOI: 10.3969/j.issn.1004-1524.2021.12.10
    Abstract ( 1430 )   HTML ( 917506 )   PDF (2180KB) ( 1232 )  

    To screen a set of core SSR (simple sequence repeat) primers for identifying of broccoli cultivars, 12 genetically different broccoli varieties were chosen as screening materials, combined polyacrylamide gel electrophoresis and fluorescence capillary electrophoresis techniques, 20 pairs of core SSR primers were finally selected from a total of 945 pairs of Brassica SSR primers. Using these 20 pairs of core primers, 79 polymorphic alleles were amplified from 48 common broccoli cultivars, with 3.95 polymorphic alleles and 5.55 genotypes per pair of primer in average. The polymorphism information content (PIC) value ranged from 0.302 to 0.750, with an average of 0.547, and the heterozygosity ranged from 0.350 0 to 0.784 9, with an average value of 0.608 2. Each of the fingerprints of 48 broccoli cultivars constructed with the core SSR primers was unique and could be used to characterize one cultivar, which provided a scientific basis for broccoli variety identification and resource protection.

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    Identification of quantitative trait loci controlling radish root index by QTL-seq
    HU Tianhua, WEI Qingzhen, WANG Jinglei, WANG Wuhong, HU Haijiao, YAN Yaqin, BAO Chonglai
    2021, 33(12):  2313-2319.  DOI: 10.3969/j.issn.1004-1524.2021.12.11
    Abstract ( 654 )   HTML ( 16385 )   PDF (2499KB) ( 1113 )  

    In order to detect QTL loci and find the key genes related to radish root shape index, an F2 population was constructed by crossing the small-sized oblate spheroidal radish LLYH and big-sized long white radish CLA which had significant differences in root shape index. QTL mapping for root shape index traits was conducted using QTL-Seq method. Four QTL loci were detected, of which two major QTL loci rs2.1 and rs2.2 were located in the 21.66-26.03 Mb and 30.79-36.56 Mb regions on chromosome R2, respectively; two minor QTL loci rs4.1 and rs6.1 were located on chromosome R4 39.04-40.43 Mb and R6 11.53-13.40 Mb.These results laid a foundation for the development of molecular markers linked to root shape index and provided an important reference for fine mapping the regulatory genes of the root shape index.

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    Comprehensive evaluation of cherry tomato quality based on principal component analysis and cluster analysis
    ZHOU Yanchao, XUE Kun, GE Haiyan, CHEN Huoying, LIU Yang
    2021, 33(12):  2320-2329.  DOI: 10.3969/j.issn.1004-1524.2021.12.12
    Abstract ( 639 )   HTML ( 9219 )   PDF (1071KB) ( 1282 )  

    In order to explore the differences in quality between breeding materials and select suitable varieties, this experiment measured 14 quality indicators of 29 cherry tomatoes, using descriptive analysis, principal component analysis, cluster analysis and discriminant analysis to evaluate the quality of cherry tomatoes. The results showed that the coefficient of variation of lycopene was the largest, reaching 59%, and the coefficient of variation of transverse diameter was only 11%. The principal component analysis simplified 14 quality indicators into 5 factors, whose cumulative variance contribution rate reached 79.05%. Combined with cluster analysis, six indicators for determining the comprehensive quality of tomato were selected: single fruit weight, sugar-acid ratio, hardness, fruit shape index, VC and lycopene. Comprehensive principal component analysis was used to screen out the hybrid combination which had the higher comprehensive quality score. Cluster analysis divided the materials into 6 categories. This study had constructed the discriminant function of cherry tomato combined with discriminant analysis. The model cross-validation showed that the correct discriminant rate reached 79.3%. This study provided new ideas for the quality evaluation of cherry tomatoes.

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    Identification, artificial cultivation and nutritional analysis of wild Pholiota adiposa
    CHEN Runchen, WANG Yining, LIU Xiaowen, WANG Hongyan, DING Qiang, WANG Honglei
    2021, 33(12):  2330-2338.  DOI: 10.3969/j.issn.1004-1524.2021.12.13
    Abstract ( 571 )   HTML ( 167772163 )   PDF (7654KB) ( 1281 )  

    In order to enrich the cultivated varieties of edible fungi, a wild fungus was identified by morphological and molecular biological methods, biological characteristics of hyphae were studied, artificial cultivation experiments were carried out, and nutritional components of fruiting bodies were determined. The results showed that the fungus was identified as Pholiota adiposa. The optimal growth temperature was 25 ℃, the optimal pH value was 5.0, and the optimal carbon source, nitrogen source and inorganic salt were sucrose, soybean cake powder and MnSO4, respectively. Fruiting bodies were successfully cultivated from agricultural wastes such as sawdust and corncob, it took about 70 days from inoculation to harvest. Protein content, polysaccharide content, fat content and free amino acid content of fruit body were 19.94%, 5.14%, 2.32% and 2.84%, respectively.

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    Plant Protection
    Effect of seed soaking with exogenous calcium on activities of defense enzymes in wheat and detoxifying enzymes in Schizaphis graminum (Rondani)
    WANG Jun, WU Xiaobao, SONG Jia, DENG Qianqian, ZENG Guang, REN Mingjian, YE Mao
    2021, 33(12):  2339-2347.  DOI: 10.3969/j.issn.1004-1524.2021.12.14
    Abstract ( 691 )   HTML ( 9 )   PDF (856KB) ( 1121 )  

    In order to study the interaction mediated by exogenous calcium between Triticum aestivum L. and Schizaphis graminum(Rondani), wheat seeds were soaked with calcium chloride solution (CaCl2 treatment), while seeds soaked with distilled water were taken as control (CK). The changes of peroxidase (POD), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL) and β-1,3-glucanase (β-1,3-GA) activity in wheat leaves were detected at 0, 24, 48, 72 h after aphids infestation. Meanwhile, glutathione S-transferase (GSTs), carboxylesterase (CarE) and cytochrome P450 (CYP450) activities in aphids fed on wheat plant for 0, 24, 48, 72 h were tested as well. It was found that the aphids infestation significantly (P<0.05) induced POD, PAL and β-1,3-GA activities in wheat leaves, but PPO activity did not increase, while CaCl2 treatment further significantly (P<0.05) enhanced activities of POD, PAL and β-1,3-GA. Besides,PPO activity in wheat plants was significantly (P<0.05) higher under CaCl2 treatment than that under CK. Meanwhile, the activities of detoxification enzymes (GSTs, CarE and CYP450) in aphids fed on CaCl2-treated plants were significantly (P<0.05) increased as compared with those fed on control plants. Collectively, the present results indicated that the exogenous calcium could induce the activities of defensive enzymes in wheat plants, while aphids increased their detoxification enzymes activities to adapt the enhanced defense of wheat.

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    Environmental Science
    Responses of soil nutrients and microbial community to altitude in typical Pinus yunnanensis forest at rocky desertification region
    SUI Xiran, WANG Yan, LIU Yungen, ZHANG Yajie, WU Lifang
    2021, 33(12):  2348-2357.  DOI: 10.3969/j.issn.1004-1524.2021.12.15
    Abstract ( 580 )   HTML ( 319 )   PDF (1227KB) ( 1199 )  

    In the present study, typical Pinus yunnanensis forest at rocky desertification region in southwest China was selected as the study area, analysis of variance (ANOVA), redundancy analysis (RDA) and high-throughput sequencing methods were applied to explore the response of soil nutrients and bacterial community composition and diversity to different altitudes, which would be helpful for the rocky desertification control and ecosystem vegetation restoration in this area. It was shown that the contents of soil total carbon, total nitrogen and total phosphorus increased fist and decreased then with increasing altitude. The highest content of soil total potassium was at altitude of 1 900 m, yet the lowest was at 1 600 m.The highest content of soil available nitrogen and available phosphorus was both at altitude of 1 600 m, while the lowest was at 1 300 m and 1 900 m, respectively. The highest content of available potassium was at altitude of 1 900 m, yet the lowest was at 1 600 m.The indexes of Chao1, Shannon increased first and then decreased with the elevated altitude, while the Simpson index showed the opposite trend. The dominant phyla of microbial community were Proteus, Acidobacteria, Actinomycetes and Curvularia, which accounted for 33.37%, 24.40%, 19.82%, 12.06%, respectively.The relative abundance of Proteus and Acidobacteria increased first and then decreased with the elevated altitude, while Curvularia showed the opposite trend. The highest relative abundance of Actinomycetes was found at altitude of 1 600 m, yet there was no significant difference within other altitudes. At class level, the dominant microbial community were Alphaproteobacteria, Acidobacteria, Gammaproteobacteria, Deltaproteobacteria, which accounted for 18.46%, 12.72%, 7.88%, 5.13%, respectively. The relative abundance of Alphaproteobacteria and Gammaproteobacteria increased first and then decreased, while Acidobacteria exhibited the opposite.There was no significant difference in the relative abundance of Deltaproteobacteria within different altitudes. Soil pH, and contents of total carbon, total nitrogen, total phosphorus, available nitrogen and available phosphorus were significantly (P<0.05) correlated with the bacterial diversity, and were main driving factors of bacterial community composition.

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    Field-scale estimation of humic acid content based on airborne hyperspectral data
    GUO Han, XU Minxian, XU Feifei, LUO Ming, LU Zhou, ZHANG Xu
    2021, 33(12):  2358-2369.  DOI: 10.3969/j.issn.1004-1524.2021.12.16
    Abstract ( 587 )   HTML ( 3 )   PDF (8517KB) ( 1052 )  

    In the present study, the airborne hyperspectral data were selected as the data source, and the spectra in the study area were transformed by continuum removal(CR), inversion recovery(IR), logistic regression(LR), first derivative reflectance(FDR), second derivative reflectance(SDR), inversion first derivative reflectance(IFDR), logarithm first derivative reflectance(LFDR), inversion logarithm regression(ILR), respectively,to construct normalized difference spectral index (NDSI), and these constructed NDSI data were denoted as NDSI-CR, NDSI-IR, NDSI-LR, NDSI-FDR, NDSI-SDR, NDSI-IFDR, NDSI-LFDR, NDSI-ILR, respectively. The correlation between NDSI and humic acid content was analyzed to identify the characteristic spectra. On this basis, multiple linear regression (MLR), partial least squares (PLSR), back propagation neural network (BPNN) and support vector machine (SVM) models were introduced to construct prediction models. The coefficient of determination (R2), root mean squared error (RMSE) and ratio of performance-to-deviation (RPD) were used as model evaluation indexes to select the best modeling method for the estimation of humic acid content at the field scale. It was shown that NDSI-FDR, NDSI-SDR, NDSI-IFDR, NDSI-LFDR had a higher correlation with humic acid content. In 396-1 000 nm, there were three sensitive band intensive regions with the humic acid content, which were located in the coordinate regions of 480-550 nm and 510-570 nm, 730-790 nm and 740-800 nm, and 880-930 nm and 880-930 nm.For the established BPNN model based on NDSI-LFDR, its R 2 on the modeling set and validation set was 0.916 and 0.805, respectively, its RMSE on the modeling set and validation set was 0.799 and 1.107, respectively, and its RPD was 2.189, which could satisfy the requirement of field-scale estimation of humic acid content.

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    Effects of different water and fertilization on nutrient uptake, yield and quality of greenhouse muskmelon under drip irrigation condition
    YUE Wenjun, HE Wenxue, DING Chunmei, BAI Yu, ZHOU Yingjie, XI Hui
    2021, 33(12):  2370-2380.  DOI: 10.3969/j.issn.1004-1524.2021.12.17
    Abstract ( 739 )   HTML ( 45064 )   PDF (1241KB) ( 1166 )  

    Water and nitrogen are two important factors affecting crop growth. In facility agriculture, farmers blindly irrigate and fertilize for obtaining high yield, which leads to lots of problems such as reduced yield, decreased quality, soil salinization, and soil fertility destruction. Aiming at the problem of unreasonable irrigation and fertilization of muskmelon in greenhouse in Hangjiahu area of southeast China, effects of different water and nitrogen inputs on dry matter accumulation, nutrient absorption and distribution, yield and quality of melon in greenhouse were studied by drip fertilization under membrane. It can provide a theoretical basis for high quality and high yield of melon and efficient utilization of water and fertilizer in greenhouse. According to data from a mini-meteorological station inside the greenhouse, crop water requirement (ETc) was calculated by modified Penman-Monteith formula. There were 60% of ETc (W1), 80% of ETc (W2), 100% of ETc (W3) three water levels and 70 (N1), 105 (N2), 140 kg·hm-2 (N3) three nitrogen levels, setting up conventional furrow irrigation fertilization CK (ETc, 140 kg·hm-2) for comparison, a total of 10 treatments, application completely randomized block design of experiment. Compared with traditional furrow fertilization (CK), the net photosynthetic rate and chlorophyll content of melon leaves of treatment W3N3 under drip fertilization was increased by 52.3% and 39.0%, respectively. The dry matter increased by 40.96 g (39%) in the mature period. The accumulations of nitrogen, phosphorus and potassium of a whole plant increased by 75.40%, 88.20% and 67.08%, respectively. Yield of melon was increased by 6.78 t·hm-2, or 35.27%. Under drip irrigation, increasing irrigation and nitrogen application can significantly improve photosynthetic efficiency, increase dry matter accumulation and nutrient absorption, and thus increase yield. The principle component analysis method was used to evaluate the muskmelon quality, and the results showed that comprehensive principle component could represent 91.27% total quality index, the highest comprehensive evaluation was obtained in W2N2. The integrated drip irrigation and fertilizer technology of melon in greenhouse can achieve the purpose of improving quality and increasing yield as well as efficient utilization of water and fertilizer. Under the condition of drip irrigation and fertilization, the amount of nitrogen applied is N3 (140 kg·hm-2) and the amount of irrigation water is W3 (1.0 ETc), the amount of dry matter accumulation, nutrient absorption and yield of melon were the highest. When both yield and fertilizer utilization efficiency were pursued, W3N2 treatment could obtain higher yield and nitrogen partial productivity. When it came to melon quality and water use efficiency (WUE), W2N2 treatment maximized VC, soluble sugar, soluble solids and high WUE.

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    Food Science
    Study on alkaline deastringency of hickory kernel
    FAN Simin, MU Honglei, GAO Haiyan, CHEN Hangjun, FANG Xiangjun, WU Weijie
    2021, 33(12):  2381-2389.  DOI: 10.3969/j.issn.1004-1524.2021.12.18
    Abstract ( 736 )   HTML ( 458758 )   PDF (2018KB) ( 1108 )  

    In order to explore a suitable alkaline deastringency method for hickory kernel, based on the response value (consisting of both deastringency rate and sensory score), single-factor tests and response surface test were adopted to optimize its technical parameters. Besides, the effects of both the proposed alkaline deastringency method and the traditional boiling-water deastringency method on the contents of free phenol and bound phenol, peroxide value, acid value and iodine value were explored. It was shown that the optimized technical parameters were as follows: deastrinency temperature of 65 ℃, deastrinency time of 100 min, NaHCO3 mass fraction of 5%, solid-liquid ratio (g·mL-1) of 1∶15. Under this condition, the deastringency rate was 92.04%, sensory score was 9.2, and the response value was 75.47. Compared with the traditional boiling-water deastringency method, the proposed alkaline deastringency method could remove astringency from hickory kernel, retain more bound phenol, reduce the oxidization of fat, fatty acid and unsaturated fatty acid, and maintain the quality of hickory kernel.

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    Optimization of preparation process of walnut (Carya cathayensis Sarg.) oil gel
    ZHOU Qiang, HAN Yanchao, WU Weijie, DING Yuting, SHAO Ping, TONG Chuan, GAO Haiyan
    2021, 33(12):  2390-2396.  DOI: 10.3969/j.issn.1004-1524.2021.12.19
    Abstract ( 647 )   HTML ( 2 )   PDF (782KB) ( 1181 )  

    Walnut (Carya cathayensis Sarg.) oil is rich in oleic acid and linoleic acid, which can reduce blood lipid content, prevent cardiovascular diseases, and has anti-oxidation effects. However, it is extremely susceptible to environmental influences to oxidize unsaturated fatty acids. Grease gel can prevent its oxidation. The research used glyceryl monostearate as gelling agent and walnut oil as carrier oil to prepare walnut oil gel. The influence of different process conditions on walnut oil gel was studied. The results showed that the influence of various factors on the walnut oil gel was as follows: gelling agent addition amount>heating temperature>heating time. The process conditions of gelling agent addition of 12%, heating time of 30 min, and heating temperature of 80 ℃ were relatively good. The peroxide value of the grease gel prepared under all process conditions meets the regulations in GB 15196-2015.

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    Effect of inoculation of Schizosaccharomyces pombe on quality of Pinot Noir dry red wine
    GAO Pingping, WEN Huating, ZHAO Mei, WANG Jing
    2021, 33(12):  2397-2405.  DOI: 10.3969/j.issn.1004-1524.2021.12.20
    Abstract ( 671 )   HTML ( 4 )   PDF (1045KB) ( 1153 )  

    In order to improve the quality of Pinot Noir dry red wine, Pinot Noir grape was selected as raw material, single strain of Schizosaccharomyces pombe (S. pombe) was used to inoculate and fermentate in alcohol with commercial Saccharomyces cerevisiae(S. cerevisiae) simultaneously and sequentially, and S. cerevisiae alone was inoculated with alcoholic fermentation followed by malolactic fermentation as the control. The changes of malic acid and biogenic amines during the fermentation process were monitored. Headspace solid phase microextraction combined with gas chromatography-mass spectrometry was used to analyze the changes of volatile aroma substances, and the sensory evaluation was carried out. The results showed that the fermented residual sugar, alcohol content, total acid and volatile acid of S. pombe inoculated simultaneously and sequentially with S. cerevisiae all met the requirements of national standard GB15037-2006 “Wine” for dry red wine. Alcohol fermentation could be realized by S. pombe alone and simultaneously or sequentially with S. cerevisiae. Compared with the control group, the inoculation method in the treated group could significantly reduce the content of malic acid and histamine in wine, among which, the contents of malic acid and histamine in the wine sequentially by S. pombe and S. cerevisiae were significantly lower than those inoculated by S. pombe alone and simultaneously with S. cerevisiae. The sequential inoculation fermentation of S. pombe and S. cerevisiae can significantly enrich the alcohols, esters and terpenes in the wine, so that the wine has strong fruit aroma and floral fragrance. Sensory analysis showed that the sensory quality of S. pombe and S. cerevisiae wines sequentially was superior to that of other treatment groups. In summary, sequential inoculation fermentation of S. pombe and S. cerevisiae can increase the alcohol, ester and terpene substances in Pinot Noir dry red wine, reduce the content of malic acid and improve the taste of wine.

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    Study on black chokeberry juicing processing and its quality evaluation
    TANG Xiaoshu, HU Bo, CHEN Xuemei, ZHANG Baixi
    2021, 33(12):  2406-2414.  DOI: 10.3969/j.issn.1004-1524.2021.12.21
    Abstract ( 734 )   HTML ( 5120 )   PDF (1579KB) ( 1213 )  

    To optimize the process of juicing to prepare black chokeberry juices with strong stability, high antioxidant capacity and good sensory quality, the solid-liquid ratio and pectinase addition were optimized based on evaluation indicators of juice yield, soluble solid and precipitation rate. Total antioxidant activity, hydroxyl radical inhibition ability and DPPH scavenging ability of optimized juice were analyzed. Meanwhile, stability of black chokeberry juice under different storage conditions was studied in terms of color, content of total phenolics and anthocyanin, and antioxidant activity. Sensory evaluation of juice was carried out by fuzzy mathematics.The results reflected theoptimumprocess of black chokeberry juice was set as solid-liquid ratio of 1∶1 and addition of pectinase at 0.4%. Total antioxidant activity was 11.5 μmol·mL-1 and ability to inhibit hydroxyl radical was 17 610.3 U·mL-1 in black chokeberry juice. Content of vitamin C (VC) of black chokeberry juice was 10.00 mg·L-1.Contents of total phenolics and anthocyanin of black chokeberry juices were 6.8 mg·mL-1 and 0.7 mg·mL-1, respectively. And after being stored at 4 ℃ in dark for 120 d, juice was dark purple red, and contents of total phenolics, anthocyanins and antioxidant capacity showed no obvious decrease. The sensory evaluation was between excellent and good. The results showed that the prepared black chokeberry juice was of high antioxidant and stable as well as good sensory quality.

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    Antihypertensive active components in edible parts of different varieties of celeries and their in vitro angiotensin converting enzyme inhibitory activities
    MA Zijia, ZHANG Kaijie, LU Shengmin, XU Zhihao, ZHENG Meiyu, LIU Zhe, WANG Lu, YANG Ying
    2021, 33(12):  2415-2422.  DOI: 10.3969/j.issn.1004-1524.2021.12.22
    Abstract ( 1399 )   HTML ( 14 )   PDF (1133KB) ( 1073 )  

    Nineteen varieties of celeries, including Green stalk celery, White celery, Purple celery, etc. were taken as research materials. Water extracts of the edible parts (stems and leaves) of the celeries investigated were prepared under the same conditions(1∶30 g·mL-1in solid-liquid ratio, 2 h in extraction time and 60 ℃ in extraction temperature) to screen out the varieties and their edible parts with higher in vitro angiotensin converting enzyme (ACE) inhibitory activity, total phenols and total flavonoids contents. The results indicated that in vitro ACE inhibitory activities of celery leaves extracted with water were significantly higher than those with ethanol solution (P<0.05). The in vitro ACE inhibitory activities in leaf aqueous extracts of different celery varieties concentrated in 80%-100%; total flavonoids contents were focused on 200-250 mg·L -1, with highest in White-stalk celery leaf and lowest in White celery leaf; total polyphenols contents were focused on 130-150 mg·L-1, with highest in Purple celery leaf and lowest in Hollow stalk green celery. Correlation analysis indicated that in vitro ACE inhibitory activity was positively related with both of total flavonoids content and total polyphenols content, with their correlation coefficients being 0.3-0.6. Green stalk celery leaf had the lowest half inhibitory concentration (IC50) values of ACE (1.258 mg·mL -1), followed by Four season celery (1.368 mg·mL-1) and White celery leaf (1.829 mg·mL-1).

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    Agricultural Economy and Development
    Relationship within capital endowment, technology cognition and farmers’ low carbon use intention of cultivated land
    WU Yaqun, DONG Jie
    2021, 33(12):  2423-2434.  DOI: 10.3969/j.issn.1004-1524.2021.12.23
    Abstract ( 575 )   HTML ( 26881 )   PDF (1267KB) ( 1114 )  

    Low carbon utilization of cultivated land is of positive significance to the reduction of greenhouse gas emissions and sustainable and healthy development of agriculture. In the present study, the theoretical analysis model and research hypothesis of capital endowment and farmers’ cognition were established first. Based on the survey data of 347 households in Hubei Province, China, farmers’ capital endowment and cognition of low-carbon utilization of cultivated land were measured. On this basis, Logit model, intermediary effect model and adjustment effect test method were adopted to reveal the relationship within capital endowment, technology cognition and farmers’ low-carbon use intention of cultivated land. It was shown that the capital endowment not only had a significant (at 1% level) direct positive impact on low-carbon use intention of cultivated land, but also had a significant (at 1% level) indirect positive impact via the intermediary variable of farmers’ cognition of low-carbon use of cultivated land. Environmental literacy played a positive moderating role between farmers’ self-efficacy cognition and low carbon use intention of cultivated land. Therefore, it was necessary to improve the farmers’ capital endowment from aspects of economic capital, social capital and cultural capital, to improve farmers’ awareness of low-carbon utilization of cultivated land through various channels, and to strengthen the popularization of environmental protection related knowledge to improve farmers’ environmental literacy.

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    Impact of Internet development in rural area on total factor productivity of maize in China
    WANG Bo, ZHANG Yongqiang, GONG Siyu, DONG Quanyao, FU Xiaozhao
    2021, 33(12):  2435-2445.  DOI: 10.3969/j.issn.1004-1524.2021.12.24
    Abstract ( 686 )   HTML ( 1281 )   PDF (1193KB) ( 1130 )  

    In the present study, the DEA-Mamlquist index was introduced to estimate the total factor productivity of 17 major maize producing provinces (autonomous regions) in China from 2004 to 2019,and the simultaneous equations model was uesd to test the impact and reveal the mechanism of Internet development in rural area on the total factor productivity of maize. It was shown that the average annual growth rate of maize total factor productivity in 2004-2019 was 0.2%, which was mainly attributed to the technical progress. The Internet development in rural area has significantly (P<0.01) increased the total factor productivity of maize, which was mainly attributed to both the technical progress and technical efficiency. In different ecological areas, the Internet development in rural area has significant (P<0.01) promotion effect on the total factor productivity of maize, and the influence decreased as spring maize planting area in north China>summer maize planting area in Huang-Huai-Hai Plain>northwest irrigated maize area>southwest mountainous maize area. Accordingly, it was suggested to improve the construction of the rural Internet supporting infrastructure, take the advantage of the “connectivity economy” of the Internet, and diversify Internet technologies to promote maize total factor productivity in different ecological areas.

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    Review
    Research progress on genetic and regulatory mechanisms of sucrose in vegetable soybean seeds
    ZHANG Weimei, ZHANG Guwen, FENG Zhijuan, LIU Na, WANG Bin, BU Yuanpeng
    2021, 33(12):  2446-2456.  DOI: 10.3969/j.issn.1004-1524.2021.12.25
    Abstract ( 735 )   HTML ( 90118 )   PDF (1066KB) ( 1269 )  

    Sweetness is an important aspect of vegetable soybean, and it is one of the distinguishing characteristics of vegetable soybean from ordinary grain-type soybean. It is generally believed that the sweetness of vegetable soybean is mainly attributed to the soluble sugar content. Sucrose accounts for 71% of the total soluble sugar, which is the key factor determining the sweetness and taste quality score of vegetable soybean. Understanding the genetic and regulatory mechanism of sucrose in vegetable soybean seeds is of great significance for accelerating the genetic improvement of taste quality and enhancing the competitiveness of Chinese vegetable soybean in the international market. This article reviewed the research progress on the metabolism and regulation of sucrose in vegetable soybean grains, mainly on the genetic mechanism of soybean sucrose content, the regulation mechanism of key enzymes in the process of sucrose metabolism and the cloning of their homologous genes. Finally, the future research directions and goals of vegetable soybean sucrose content were prospected in order to provide reference for improving the eating quality of vegetable soybean.

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